(Circular) (Six-base) MAPSORT of: pF1KB9385.seq Check: 7163 from: 1 to: 4085 pF1KB9385 4085 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AccI GT'mk_AC Cuts at: 1123 1123 Size: 4085 Acc65I G'GTAC_C Cuts at: 929 1101 929 Size: 172 3913 AclI AA'CG_TT Cuts at: 3925 3925 Size: 4085 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 942 1049 1910 2342 3089 3357 942 Size: 107 861 432 747 268 1670 Fragments arranged by size: 1670 861 747 432 268 107 AflIII A'CryG_T Cuts at: 215 1437 2541 3518 215 Size: 1222 1104 977 782 Fragments arranged by size: 1222 1104 977 782 AgeI A'CCGG_T Cuts at: 112 2473 112 Size: 2361 1724 AleI CACnn'nnGTG Cuts at: 220 220 Size: 4085 AlwNI CAG_nnn'CTG Cuts at: 195 544 1390 2957 195 Size: 349 846 1567 1323 Fragments arranged by size: 1567 1323 846 349 ApaLI G'TGCA_C Cuts at: 2855 2855 Size: 4085 ApoI r'AATT_y Cuts at: 1089 3438 3694 1089 Size: 2349 256 1480 Fragments arranged by size: 2349 1480 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4085 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4085 AvaI C'yCGr_G Cuts at: 145 1105 145 Size: 960 3125 BamHI G'GATC_C Cuts at: 477 891 1110 477 Size: 414 219 3452 Fragments arranged by size: 3452 414 219 BanI G'GyrC_C Cuts at: 277 929 1101 1515 1763 1798 277 Size: 652 172 414 248 35 2564 Fragments arranged by size: 2564 652 414 248 172 35 BanII G_rGCy'C Cuts at: 1099 2129 1099 Size: 1030 3055 BbeI G_GCGC'C Cuts at: 281 1519 1767 281 Size: 1238 248 2599 Fragments arranged by size: 2599 1238 248 BciVI GTATCCnnnnn_n' Cuts at: 1983 2744 4085 1983 Size: 761 1341 1983 Fragments arranged by size: 1983 1341 761 BclI T'GATC_A Cuts at: 366 1605 366 Size: 1239 2846 BfrBI ATG'CAT Cuts at: 351 351 Size: 4085 BglII A'GATC_T Cuts at: 1600 3637 1600 Size: 2037 2048 BlpI GC'TnA_GC Cuts at: 1197 1197 Size: 4085 Bme1580I G_kGCm'C Cuts at: 1710 1803 2859 1710 Size: 93 1056 2936 Fragments arranged by size: 2936 1056 93 BmgBI CAC'GTC Cuts at: 218 218 Size: 4085 BmrI ACTGGGnnnn_n' Cuts at: 183 808 1297 1378 1713 3857 183 Size: 625 489 81 335 2144 411 Fragments arranged by size: 2144 625 489 411 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2452 2452 Size: 4085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 339 566 339 Size: 227 3858 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 244 1261 2140 2632 2930 244 Size: 1017 879 492 298 1399 Fragments arranged by size: 1399 1017 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 542 3789 542 Size: 3247 838 BsaAI yAC'GTr Cuts at: 615 1438 615 Size: 823 3262 BsaBI GATnn'nnATC Cuts at: 6 1599 3636 6 Size: 1593 2037 455 Fragments arranged by size: 2037 1593 455 BsaHI Gr'CG_yC Cuts at: 278 1516 1764 2466 3962 278 Size: 1238 248 702 1496 401 Fragments arranged by size: 1496 1238 702 401 248 BsaWI w'CCGG_w Cuts at: 112 1274 1795 2473 2747 2894 3305 112 Size: 1162 521 678 274 147 411 892 Fragments arranged by size: 1162 892 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 150 195 150 Size: 45 4040 BseYI C'CCAG_C Cuts at: 499 1510 2268 2845 3341 499 Size: 1011 758 577 496 1243 Fragments arranged by size: 1243 1011 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 637 637 Size: 4085 BsiEI CG_ry'CG Cuts at: 104 1627 1673 2881 104 Size: 1523 46 1208 1308 Fragments arranged by size: 1523 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1099 1877 2067 2859 1099 Size: 778 190 792 2325 Fragments arranged by size: 2325 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 232 232 Size: 4085 Bsp1286I G_dGCh'C Cuts at: 1099 1710 1803 1877 2067 2129 2859 1099 Size: 611 93 74 190 62 730 2325 Fragments arranged by size: 2325 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3305 3305 Size: 4085 BspHI T'CATG_A Cuts at: 3261 3261 Size: 4085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1135 1349 1651 2032 1135 Size: 214 302 381 3188 Fragments arranged by size: 3188 381 302 214 BsrBI CCG'CTC Cuts at: 50 2377 2431 3915 4080 50 Size: 2327 54 1484 165 55 Fragments arranged by size: 2327 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1997 1997 Size: 4085 BsrFI r'CCGG_y Cuts at: 112 240 274 551 2083 2264 2473 112 Size: 128 34 277 1532 181 209 1724 Fragments arranged by size: 1724 1532 277 209 181 128 34 BssSI C'ACGA_G Cuts at: 936 2356 2714 936 Size: 1420 358 2307 Fragments arranged by size: 2307 1420 358 BstAPI GCAn_nnn'nTGC Cuts at: 952 1462 952 Size: 510 3575 BstBI TT'CG_AA Cuts at: 2446 2446 Size: 4085 BstYI r'GATC_y Cuts at: 1 477 891 1110 1577 1600 1935 2181 3193 Size: 476 414 219 467 23 335 246 1012 Cuts at: 3193 3279 3291 3637 1 Size: 86 12 346 449 Fragments arranged by size: 1012 476 467 449 414 346 335 246 219 86 23 12 Bsu36I CC'TnA_GG Cuts at: 743 743 Size: 4085 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 927 2035 2216 2379 927 Size: 1108 181 163 2633 Fragments arranged by size: 2633 1108 181 163 BtsI GCAGTG_nn' Cuts at: 1328 1328 Size: 4085 DraI TTT'AAA Cuts at: 1084 1084 Size: 4085 DrdI GACnn_nn'nnGTC Cuts at: 1791 2649 1791 Size: 858 3227 EaeI y'GGCC_r Cuts at: 242 1670 1844 2235 2262 3607 242 Size: 1428 174 391 27 1345 720 Fragments arranged by size: 1428 1345 720 391 174 27 EagI C'GGCC_G Cuts at: 1670 1670 Size: 4085 EarI CTCTTCn'nnn_ Cuts at: 2108 2318 2108 Size: 210 3875 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2293 2603 2749 3895 2293 Size: 310 146 1146 2483 Fragments arranged by size: 2483 1146 310 146 EcoICRI GAG'CTC Cuts at: 1097 1097 Size: 4085 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 339 566 942 1049 1910 2342 3089 3357 339 Size: 227 376 107 861 432 747 268 1067 Fragments arranged by size: 1067 861 747 432 376 268 227 107 EcoO109I rG'GnC_Cy Cuts at: 386 503 1224 386 Size: 117 721 3247 Fragments arranged by size: 3247 721 117 EcoRI G'AATT_C Cuts at: 1089 1089 Size: 4085 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 394 783 2104 394 Size: 389 1321 2375 Fragments arranged by size: 2375 1321 389 FspI TGC'GCA Cuts at: 1866 1866 Size: 4085 HaeII r_GCGC'y Cuts at: 281 1519 1767 2789 3768 281 Size: 1238 248 1022 979 598 Fragments arranged by size: 1238 1022 979 598 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 142 174 495 527 1927 1957 1959 1989 142 Size: 32 321 32 1400 30 2 30 2238 Fragments arranged by size: 2238 1400 321 32 32 30 30 2 HincII GTy'rAC Cuts at: 1124 1124 Size: 4085 KasI G'GCGC_C Cuts at: 277 1515 1763 277 Size: 1238 248 2599 Fragments arranged by size: 2599 1238 248 KpnI G_GTAC'C Cuts at: 933 1105 933 Size: 172 3913 MfeI C'AATT_G Cuts at: 340 340 Size: 4085 MluI A'CGCG_T Cuts at: 3518 3518 Size: 4085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 331 2756 2940 331 Size: 2425 184 1476 Fragments arranged by size: 2425 1476 184 MscI TGG'CCA Cuts at: 1846 1846 Size: 4085 MslI CAynn'nnrTG Cuts at: 220 2074 2201 220 Size: 1854 127 2104 Fragments arranged by size: 2104 1854 127 MspA1I CmG'CkG Cuts at: 1196 1510 1870 2883 3128 1196 Size: 314 360 1013 245 2153 Fragments arranged by size: 2153 1013 360 314 245 NaeI GCC'GGC Cuts at: 242 2266 242 Size: 2024 2061 NarI GG'CG_CC Cuts at: 278 1516 1764 278 Size: 1238 248 2599 Fragments arranged by size: 2599 1238 248 NgoMIV G'CCGG_C Cuts at: 240 2264 240 Size: 2024 2061 NsiI A_TGCA'T Cuts at: 353 353 Size: 4085 NspI r_CATG'y Cuts at: 316 1138 1638 2545 316 Size: 822 500 907 1856 Fragments arranged by size: 1856 907 822 500 PciI A'CATG_T Cuts at: 2541 2541 Size: 4085 PfoI T'CCnGG_A Cuts at: 284 284 Size: 4085 PmeI GTTT'AAAC Cuts at: 1084 1084 Size: 4085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3224 3256 3224 Size: 32 4053 PpuMI rG'GwC_Cy Cuts at: 386 503 386 Size: 117 3968 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 595 627 595 Size: 32 4053 PstI C_TGCA'G Cuts at: 1132 1817 1132 Size: 685 3400 PvuI CG_AT'CG Cuts at: 104 104 Size: 4085 PvuII CAG'CTG Cuts at: 1510 1870 1510 Size: 360 3725 RsrII CG'GwC_CG Cuts at: 2280 2280 Size: 4085 SacI G_AGCT'C Cuts at: 1099 1099 Size: 4085 SalI G'TCGA_C Cuts at: 1122 1122 Size: 4085 SanDI GG'GwC_CC Cuts at: 386 386 Size: 4085 SapI GCTCTTCn'nnn_ Cuts at: 2108 2318 2108 Size: 210 3875 SbfI CC_TGCA'GG Cuts at: 1132 1132 Size: 4085 ScaI AGT'ACT Cuts at: 3602 3602 Size: 4085 SfcI C'TryA_G Cuts at: 33 1128 1813 2806 2997 33 Size: 1095 685 993 191 1121 Fragments arranged by size: 1121 1095 993 685 191 SfoI GGC'GCC Cuts at: 279 1517 1765 279 Size: 1238 248 2599 Fragments arranged by size: 2599 1238 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4085 SgrAI Cr'CCGG_yG Cuts at: 274 274 Size: 4085 SmaI CCC'GGG Cuts at: 1107 1107 Size: 4085 SmlI C'TyrA_G Cuts at: 259 1240 2155 2647 2909 259 Size: 981 915 492 262 1435 Fragments arranged by size: 1435 981 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4085 SphI G_CATG'C Cuts at: 316 1138 1638 316 Size: 822 500 2763 Fragments arranged by size: 2763 822 500 SspI AAT'ATT Cuts at: 798 885 798 Size: 87 3998 StyI C'CwwG_G Cuts at: 1076 1219 1573 1076 Size: 143 354 3588 Fragments arranged by size: 3588 354 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1668 1801 2260 2469 1668 Size: 133 459 209 3284 Fragments arranged by size: 3284 459 209 133 TatI w'GTAC_w Cuts at: 3600 3600 Size: 4085 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1008 1040 3602 3634 1008 Size: 32 2562 32 1459 Fragments arranged by size: 2562 1459 32 32 Tth111I GACn'n_nGTC Cuts at: 1882 1882 Size: 4085 XbaI T'CTAG_A Cuts at: 1116 1116 Size: 4085 XcmI CCAnnnn_n'nnnnTGG Cuts at: 314 1580 314 Size: 1266 2819 XmaI C'CCGG_G Cuts at: 1105 1105 Size: 4085 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI PfoI PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI NotI NruI PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI SacII SexAI SfiI SnaBI SrfI StuI SwaI XhoI XmnI ZraI