Restriction map for
pF1KB9394
(Circular) (Six-base) MAPSORT of: pF1KB9394.seq Check: 3425 from: 1 to: 4277
pF1KB9394 4277 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 297 665 297
Size: 368 3909
AccI GT'mk_AC
Cuts at: 1315 1315
Size: 4277
Acc65I G'GTAC_C
Cuts at: 718 1293 718
Size: 575 3702
AclI AA'CG_TT
Cuts at: 752 4117 752
Size: 3365 912
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 2102 2534 3281 3549 437
Size: 1665 432 747 268 1165
Fragments arranged by size:
1665 1165 747 432 268
AflIII A'CryG_T
Cuts at: 1629 2733 3710 1629
Size: 1104 977 2196
Fragments arranged by size:
2196 1104 977
AgeI A'CCGG_T
Cuts at: 2665 2665
Size: 4277
AlwNI CAG_nnn'CTG
Cuts at: 684 1582 3149 684
Size: 898 1567 1812
Fragments arranged by size:
1812 1567 898
ApaI G_GGCC'C
Cuts at: 478 478
Size: 4277
ApaLI G'TGCA_C
Cuts at: 3047 3047
Size: 4277
ApoI r'AATT_y
Cuts at: 1281 3630 3886 1281
Size: 2349 256 1672
Fragments arranged by size:
2349 1672 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4277
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
AvaI C'yCGr_G
Cuts at: 1246 1297 1246
Size: 51 4226
BamHI G'GATC_C
Cuts at: 1302 1302
Size: 4277
BanI G'GyrC_C
Cuts at: 210 718 1293 1707 1955 1990 210
Size: 508 575 414 248 35 2497
Fragments arranged by size:
2497 575 508 414 248 35
BanII G_rGCy'C
Cuts at: 478 1291 2321 478
Size: 813 1030 2434
Fragments arranged by size:
2434 1030 813
BbeI G_GCGC'C
Cuts at: 214 1711 1959 214
Size: 1497 248 2532
Fragments arranged by size:
2532 1497 248
BbsI GAAGACnn'nnnn_
Cuts at: 425 425
Size: 4277
BbvCI CC'TCA_GC
Cuts at: 1138 1138
Size: 4277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 295 329 90
Size: 34 171 34 4038
Fragments arranged by size:
4038 171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2175 2936 4277 2175
Size: 761 1341 2175
Fragments arranged by size:
2175 1341 761
BclI T'GATC_A
Cuts at: 1797 1797
Size: 4277
BglII A'GATC_T
Cuts at: 1792 3829 1792
Size: 2037 2240
BlpI GC'TnA_GC
Cuts at: 1389 1389
Size: 4277
Bme1580I G_kGCm'C
Cuts at: 478 1902 1995 3051 478
Size: 1424 93 1056 1704
Fragments arranged by size:
1704 1424 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 634 1489 1570 1905 4049 634
Size: 855 81 335 2144 862
Fragments arranged by size:
2144 862 855 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 4277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 655
Size: 4277
Bpu10I CC'TnA_GC
Cuts at: 293 1138 1256 293
Size: 845 118 3314
Fragments arranged by size:
3314 845 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1453 2332 2824 3122 1453
Size: 879 492 298 2608
Fragments arranged by size:
2608 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3981 3981
Size: 4277
BsaAI yAC'GTr
Cuts at: 1630 1630
Size: 4277
BsaBI GATnn'nnATC
Cuts at: 6 607 812 1791 3828 6
Size: 601 205 979 2037 455
Fragments arranged by size:
2037 979 601 455 205
BsaHI Gr'CG_yC
Cuts at: 211 1211 1708 1956 2658 4154 211
Size: 1000 497 248 702 1496 334
Fragments arranged by size:
1496 1000 702 497 334 248
BsaWI w'CCGG_w
Cuts at: 617 822 1466 1987 2665 2939 3086 3497 617
Size: 205 644 521 678 274 147 411 1397
Fragments arranged by size:
1397 678 644 521 411 274 205 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1118 1118
Size: 4277
BseYI C'CCAG_C
Cuts at: 1702 2460 3037 3533 1702
Size: 758 577 496 2446
Fragments arranged by size:
2446 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1819 1865 3073 104
Size: 1715 46 1208 1308
Fragments arranged by size:
1715 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 294 635 1291 2069 2259 3051 294
Size: 341 656 778 190 792 1520
Fragments arranged by size:
1520 792 778 656 341 190
BsmI GAATG_Cn'
Cuts at: 257 1038 257
Size: 781 3496
Bsp1286I G_dGCh'C
Cuts at: 294 478 635 1291 1902 1995 2069 2259 2321
Size: 184 157 656 611 93 74 190 62
Cuts at: 2321 3051 294
Size: 730 1520
Fragments arranged by size:
1520 730 656 611 190 184 157 93
74 62
BspEI T'CCGG_A
Cuts at: 617 3497 617
Size: 2880 1397
BspHI T'CATG_A
Cuts at: 239 519 804 3453 239
Size: 280 285 2649 1063
Fragments arranged by size:
2649 1063 285 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 297 454 665 1327 1541 1843 2224 297
Size: 157 211 662 214 302 381 2350
Fragments arranged by size:
2350 662 381 302 214 211 157
BsrBI CCG'CTC
Cuts at: 50 146 2569 2623 4107 4272 50
Size: 96 2423 54 1484 165 55
Fragments arranged by size:
2423 1484 165 96 55 54
BsrDI GCAATG_nn'
Cuts at: 776 2189 776
Size: 1413 2864
BsrFI r'CCGG_y
Cuts at: 1052 2275 2456 2665 1052
Size: 1223 181 209 2664
Fragments arranged by size:
2664 1223 209 181
BssHII G'CGCG_C
Cuts at: 111 111
Size: 4277
BssSI C'ACGA_G
Cuts at: 628 2548 2906 628
Size: 1920 358 1999
Fragments arranged by size:
1999 1920 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1654 1654
Size: 4277
BstBI TT'CG_AA
Cuts at: 2638 2638
Size: 4277
BstXI CCAn_nnnn'nTGG
Cuts at: 1120 1120
Size: 4277
BstYI r'GATC_y
Cuts at: 1 1302 1769 1792 2127 2373 3385 3471 3483
Size: 1301 467 23 335 246 1012 86 12
Cuts at: 3483 3829 1
Size: 346 449
Fragments arranged by size:
1301 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2227 2408 2571 2227
Size: 181 163 3933
Fragments arranged by size:
3933 181 163
BtsI GCAGTG_nn'
Cuts at: 348 1520 348
Size: 1172 3105
DraI TTT'AAA
Cuts at: 1276 1276
Size: 4277
DrdI GACnn_nn'nnGTC
Cuts at: 1983 2841 1983
Size: 858 3419
EaeI y'GGCC_r
Cuts at: 1054 1862 2036 2427 2454 3799 1054
Size: 808 174 391 27 1345 1532
Fragments arranged by size:
1532 1345 808 391 174 27
EagI C'GGCC_G
Cuts at: 1862 1862
Size: 4277
EarI CTCTTCn'nnn_
Cuts at: 169 241 532 2300 2510 169
Size: 72 291 1768 210 1936
Fragments arranged by size:
1936 1768 291 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 941 2485 2795 2941 4087 941
Size: 1544 310 146 1146 1131
Fragments arranged by size:
1544 1146 1131 310 146
EcoICRI GAG'CTC
Cuts at: 1289 1289
Size: 4277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 655 2102 2534 3281 3549 437
Size: 218 1447 432 747 268 1165
Fragments arranged by size:
1447 1165 747 432 268 218
EcoO109I rG'GnC_Cy
Cuts at: 474 1416 474
Size: 942 3335
EcoRI G'AATT_C
Cuts at: 1281 1281
Size: 4277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 479 2296 479
Size: 1817 2460
FspI TGC'GCA
Cuts at: 2058 2058
Size: 4277
HaeII r_GCGC'y
Cuts at: 214 678 684 1711 1959 2981 3960 214
Size: 464 6 1027 248 1022 979 531
Fragments arranged by size:
1027 1022 979 531 464 248 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1094 1126 2119 2149 2151 2181 1094
Size: 32 993 30 2 30 3190
Fragments arranged by size:
3190 993 32 30 30 2
HincII GTy'rAC
Cuts at: 1236 1316 1236
Size: 80 4197
KasI G'GCGC_C
Cuts at: 210 1707 1955 210
Size: 1497 248 2532
Fragments arranged by size:
2532 1497 248
KpnI G_GTAC'C
Cuts at: 722 1297 722
Size: 575 3702
MluI A'CGCG_T
Cuts at: 3710 3710
Size: 4277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2948 3132 2948
Size: 184 4093
MscI TGG'CCA
Cuts at: 2038 2038
Size: 4277
MslI CAynn'nnrTG
Cuts at: 490 2266 2393 490
Size: 1776 127 2374
Fragments arranged by size:
2374 1776 127
MspA1I CmG'CkG
Cuts at: 1388 1702 2062 3075 3320 1388
Size: 314 360 1013 245 2345
Fragments arranged by size:
2345 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1054 2458 1054
Size: 1404 2873
NarI GG'CG_CC
Cuts at: 211 1708 1956 211
Size: 1497 248 2532
Fragments arranged by size:
2532 1497 248
NgoMIV G'CCGG_C
Cuts at: 1052 2456 1052
Size: 1404 2873
NspI r_CATG'y
Cuts at: 585 1330 1830 2737 585
Size: 745 500 907 2125
Fragments arranged by size:
2125 907 745 500
PciI A'CATG_T
Cuts at: 2733 2733
Size: 4277
PfoI T'CCnGG_A
Cuts at: 266 266
Size: 4277
PmeI GTTT'AAAC
Cuts at: 1276 1276
Size: 4277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 4245
PspOMI G'GGCC_C
Cuts at: 474 474
Size: 4277
PstI C_TGCA'G
Cuts at: 689 1324 2009 689
Size: 635 685 2957
Fragments arranged by size:
2957 685 635
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4277
PvuII CAG'CTG
Cuts at: 1702 2062 1702
Size: 360 3917
RsrII CG'GwC_CG
Cuts at: 2472 2472
Size: 4277
SacI G_AGCT'C
Cuts at: 1291 1291
Size: 4277
SalI G'TCGA_C
Cuts at: 1314 1314
Size: 4277
SapI GCTCTTCn'nnn_
Cuts at: 169 2300 2510 169
Size: 2131 210 1936
Fragments arranged by size:
2131 1936 210
SbfI CC_TGCA'GG
Cuts at: 1324 1324
Size: 4277
ScaI AGT'ACT
Cuts at: 3794 3794
Size: 4277
SfcI C'TryA_G
Cuts at: 33 685 1320 2005 2998 3189 33
Size: 652 635 685 993 191 1121
Fragments arranged by size:
1121 993 685 652 635 191
SfoI GGC'GCC
Cuts at: 212 1709 1957 212
Size: 1497 248 2532
Fragments arranged by size:
2532 1497 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
SmaI CCC'GGG
Cuts at: 1299 1299
Size: 4277
SmlI C'TyrA_G
Cuts at: 1432 2347 2839 3101 1432
Size: 915 492 262 2608
Fragments arranged by size:
2608 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4277
SphI G_CATG'C
Cuts at: 585 1330 1830 585
Size: 745 500 3032
Fragments arranged by size:
3032 745 500
StyI C'CwwG_G
Cuts at: 252 1411 1765 252
Size: 1159 354 2764
Fragments arranged by size:
2764 1159 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1860 1993 2452 2661 1860
Size: 133 459 209 3476
Fragments arranged by size:
3476 459 209 133
TatI w'GTAC_w
Cuts at: 194 1026 1076 3792 194
Size: 832 50 2716 679
Fragments arranged by size:
2716 832 679 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 327 359 3794 3826 327
Size: 32 3435 32 778
Fragments arranged by size:
3435 778 32 32
Tth111I GACn'n_nGTC
Cuts at: 2074 2074
Size: 4277
XbaI T'CTAG_A
Cuts at: 1308 1308
Size: 4277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1772 1772
Size: 4277
XmaI C'CCGG_G
Cuts at: 1297 1297
Size: 4277
XmnI GAAnn'nnTTC
Cuts at: 953 953
Size: 4277
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmBI BsrGI BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI