Restriction map for
pF1KB9397
(Circular) (Six-base) MAPSORT of: pF1KB9397.seq Check: 5135 from: 1 to: 5063
pF1KB9397 5063 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 927 927
Size: 5063
AccI GT'mk_AC
Cuts at: 2101 2101
Size: 5063
Acc65I G'GTAC_C
Cuts at: 2079 2079
Size: 5063
AclI AA'CG_TT
Cuts at: 4903 4903
Size: 5063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 335 399 490 2888 3320 3673 4335 311
Size: 24 64 91 2398 432 353 662 1039
Fragments arranged by size:
2398 1039 662 432 353 91 64 24
AflIII A'CryG_T
Cuts at: 1271 2415 4215 4496 1271
Size: 1144 1800 281 1838
Fragments arranged by size:
1838 1800 1144 281
AlwNI CAG_nnn'CTG
Cuts at: 298 2368 3806 298
Size: 2070 1438 1555
Fragments arranged by size:
2070 1555 1438
ApaLI G'TGCA_C
Cuts at: 3901 3901
Size: 5063
ApoI r'AATT_y
Cuts at: 1009 1326 2067 4416 4672 1009
Size: 317 741 2349 256 1400
Fragments arranged by size:
2349 1400 741 317 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5063
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5063
AvaI C'yCGr_G
Cuts at: 929 1214 2083 929
Size: 285 869 3909
Fragments arranged by size:
3909 869 285
AvrII C'CTAG_G
Cuts at: 1966 1966
Size: 5063
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 502 535 502
Size: 33 5030
BamHI G'GATC_C
Cuts at: 1838 2088 1838
Size: 250 4813
BanI G'GyrC_C
Cuts at: 658 2079 2493 2741 2776 658
Size: 1421 414 248 35 2945
Fragments arranged by size:
2945 1421 414 248 35
BanII G_rGCy'C
Cuts at: 875 2077 3107 875
Size: 1202 1030 2831
Fragments arranged by size:
2831 1202 1030
BbeI G_GCGC'C
Cuts at: 2497 2745 2497
Size: 248 4815
BbsI GAAGACnn'nnnn_
Cuts at: 464 550 566 464
Size: 86 16 4961
Fragments arranged by size:
4961 86 16
BciVI GTATCCnnnnn_n'
Cuts at: 805 2961 4017 5063 805
Size: 2156 1056 1046 805
Fragments arranged by size:
2156 1056 1046 805
BclI T'GATC_A
Cuts at: 695 2583 695
Size: 1888 3175
BglI GCCn_nnn'nGGC
Cuts at: 378 378
Size: 5063
BglII A'GATC_T
Cuts at: 910 1562 2578 4615 910
Size: 652 1016 2037 1358
Fragments arranged by size:
2037 1358 1016 652
BlpI GC'TnA_GC
Cuts at: 1817 2175 1817
Size: 358 4705
Bme1580I G_kGCm'C
Cuts at: 663 2688 2781 3905 663
Size: 2025 93 1124 1821
Fragments arranged by size:
2025 1821 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1844 2275 2356 2691 4835 1844
Size: 431 81 335 2144 2072
Fragments arranged by size:
2144 2072 431 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1082 1706 3430 1082
Size: 624 1724 2715
Fragments arranged by size:
2715 1724 624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1879 1879
Size: 5063
Bpu10I CC'TnA_GC
Cuts at: 1796 1796
Size: 5063
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1077 1684 2012 2239 3118 3832 4130 1077
Size: 607 328 227 879 714 298 2010
Fragments arranged by size:
2010 879 714 607 328 298 227
BsaI GGTCTCn'nnnn_
Cuts at: 277 4767 277
Size: 4490 573
BsaAI yAC'GTr
Cuts at: 2416 2416
Size: 5063
BsaBI GATnn'nnATC
Cuts at: 6 1151 2577 4614 6
Size: 1145 1426 2037 455
Fragments arranged by size:
2037 1426 1145 455
BsaHI Gr'CG_yC
Cuts at: 112 1045 2494 2742 3444 4940 112
Size: 933 1449 248 702 1496 235
Fragments arranged by size:
1496 1449 933 702 248 235
BsaWI w'CCGG_w
Cuts at: 1841 2252 2773 3451 3862 4009 4283 1841
Size: 411 521 678 411 147 274 2621
Fragments arranged by size:
2621 678 521 411 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2002 2032 2002
Size: 30 5033
BseYI C'CCAG_C
Cuts at: 2488 3246 3911 4319 2488
Size: 758 665 408 3232
Fragments arranged by size:
3232 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 905 905
Size: 5063
BsiEI CG_ry'CG
Cuts at: 104 2605 2651 3881 104
Size: 2501 46 1230 1286
Fragments arranged by size:
2501 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1960 2077 2855 3045 3905 1960
Size: 117 778 190 860 3118
Fragments arranged by size:
3118 860 778 190 117
BsmI GAATG_Cn'
Cuts at: 257 257
Size: 5063
BsmBI CGTCTCn'nnnn_
Cuts at: 1967 1967
Size: 5063
Bsp1286I G_dGCh'C
Cuts at: 663 875 1960 2077 2688 2781 2855 3045 3107
Size: 212 1085 117 611 93 74 190 62
Cuts at: 3107 3905 663
Size: 798 1821
Fragments arranged by size:
1821 1085 798 611 212 190 117 93
74 62
BspEI T'CCGG_A
Cuts at: 1841 1841
Size: 5063
BspHI T'CATG_A
Cuts at: 3495 3495
Size: 5063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 306 927 2113 2327 2629 3010 306
Size: 621 1186 214 302 381 2359
Fragments arranged by size:
2359 1186 621 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1869 3355 3409 4893 5058 50
Size: 1819 1486 54 1484 165 55
Fragments arranged by size:
1819 1486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 190 1562 2975 190
Size: 1372 1413 2278
Fragments arranged by size:
2278 1413 1372
BsrFI r'CCGG_y
Cuts at: 3061 3242 3061
Size: 181 4882
BssSI C'ACGA_G
Cuts at: 1693 3334 4042 1693
Size: 1641 708 2714
Fragments arranged by size:
2714 1641 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1788 2440 1788
Size: 652 4411
BstBI TT'CG_AA
Cuts at: 3424 3424
Size: 5063
BstYI r'GATC_y
Cuts at: 1 910 1347 1562 1838 2088 2555 2578 2913
Size: 909 437 215 276 250 467 23 335
Cuts at: 2913 3159 3465 3477 3563 4615 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 909 467 449 437 335 306 276
250 246 215 86 23 12
BtgI C'CryG_G
Cuts at: 107 1933 107
Size: 1826 3237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 995 3013 3194 3357 995
Size: 2018 181 163 2701
Fragments arranged by size:
2701 2018 181 163
BtsI GCAGTG_nn'
Cuts at: 1413 2306 1413
Size: 893 4170
DraI TTT'AAA
Cuts at: 2062 2062
Size: 5063
DraIII CAC_nnn'GTG
Cuts at: 1388 1388
Size: 5063
DrdI GACnn_nn'nnGTC
Cuts at: 568 2769 4113 568
Size: 2201 1344 1518
Fragments arranged by size:
2201 1518 1344
EaeI y'GGCC_r
Cuts at: 826 1880 2648 2822 3213 3240 4585 826
Size: 1054 768 174 391 27 1345 1304
Fragments arranged by size:
1345 1304 1054 768 391 174 27
EagI C'GGCC_G
Cuts at: 2648 2648
Size: 5063
EarI CTCTTCn'nnn_
Cuts at: 348 1624 1742 3086 3296 348
Size: 1276 118 1344 210 2115
Fragments arranged by size:
2115 1344 1276 210 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 157 3271 4013 4159 4873 157
Size: 3114 742 146 714 347
Fragments arranged by size:
3114 742 714 347 146
EcoICRI GAG'CTC
Cuts at: 2075 2075
Size: 5063
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 335 399 490 1879 2888 3320 3673 4335
Size: 24 64 91 1389 1009 432 353 662
Cuts at: 4335 311
Size: 1039
Fragments arranged by size:
1389 1039 1009 662 432 353 91 64
24
EcoNI CCTnn'n_nnAGG
Cuts at: 1965 1965
Size: 5063
EcoO109I rG'GnC_Cy
Cuts at: 295 2202 295
Size: 1907 3156
EcoRI G'AATT_C
Cuts at: 2067 2067
Size: 5063
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 929 2053 3082 929
Size: 1124 1029 2910
Fragments arranged by size:
2910 1124 1029
FspI TGC'GCA
Cuts at: 2844 2844
Size: 5063
HaeII r_GCGC'y
Cuts at: 2497 2745 3975 4746 2497
Size: 248 1230 771 2814
Fragments arranged by size:
2814 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2905 2935 2937 2967 2905
Size: 30 2 30 5001
Fragments arranged by size:
5001 30 30 2
HincII GTy'rAC
Cuts at: 1265 2102 1265
Size: 837 4226
HindIII A'AGCT_T
Cuts at: 1225 1225
Size: 5063
HpaI GTT'AAC
Cuts at: 1265 1265
Size: 5063
KasI G'GCGC_C
Cuts at: 2493 2741 2493
Size: 248 4815
KpnI G_GTAC'C
Cuts at: 2083 2083
Size: 5063
MluI A'CGCG_T
Cuts at: 4496 4496
Size: 5063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 453 920 1388 3822 4006 140
Size: 313 467 468 2434 184 1197
Fragments arranged by size:
2434 1197 468 467 313 184
MscI TGG'CCA
Cuts at: 828 1882 2824 828
Size: 1054 942 3067
Fragments arranged by size:
3067 1054 942
MslI CAynn'nnrTG
Cuts at: 656 1250 3052 3179 656
Size: 594 1802 127 2540
Fragments arranged by size:
2540 1802 594 127
MspA1I CmG'CkG
Cuts at: 720 2174 2488 2848 3632 3877 720
Size: 1454 314 360 784 245 1906
Fragments arranged by size:
1906 1454 784 360 314 245
NaeI GCC'GGC
Cuts at: 3244 3244
Size: 5063
NarI GG'CG_CC
Cuts at: 2494 2742 2494
Size: 248 4815
NcoI C'CATG_G
Cuts at: 107 1933 107
Size: 1826 3237
NdeI CA'TA_TG
Cuts at: 965 965
Size: 5063
NgoMIV G'CCGG_C
Cuts at: 3242 3242
Size: 5063
NspI r_CATG'y
Cuts at: 158 2116 2616 4219 158
Size: 1958 500 1603 1002
Fragments arranged by size:
1958 1603 1002 500
PciI A'CATG_T
Cuts at: 4215 4215
Size: 5063
PmeI GTTT'AAAC
Cuts at: 2062 2062
Size: 5063
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 5031
PpuMI rG'GwC_Cy
Cuts at: 295 295
Size: 5063
PsiI TTA'TAA
Cuts at: 1333 1333
Size: 5063
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 494 526 494
Size: 32 5031
PstI C_TGCA'G
Cuts at: 668 2110 2795 668
Size: 1442 685 2936
Fragments arranged by size:
2936 1442 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5063
PvuII CAG'CTG
Cuts at: 2488 2848 2488
Size: 360 4703
RsrII CG'GwC_CG
Cuts at: 3258 3258
Size: 5063
SacI G_AGCT'C
Cuts at: 2077 2077
Size: 5063
SalI G'TCGA_C
Cuts at: 2100 2100
Size: 5063
SapI GCTCTTCn'nnn_
Cuts at: 3086 3296 3086
Size: 210 4853
SbfI CC_TGCA'GG
Cuts at: 668 2110 668
Size: 1442 3621
ScaI AGT'ACT
Cuts at: 753 4580 753
Size: 3827 1236
SfcI C'TryA_G
Cuts at: 33 664 839 1004 2106 2791 3759 3950 33
Size: 631 175 165 1102 685 968 191 1146
Fragments arranged by size:
1146 1102 968 685 631 191 175 165
SfoI GGC'GCC
Cuts at: 2495 2743 2495
Size: 248 4815
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5063
SmaI CCC'GGG
Cuts at: 931 2085 931
Size: 1154 3909
SmlI C'TyrA_G
Cuts at: 1056 1663 2027 2218 3133 3847 4109 1056
Size: 607 364 191 915 714 262 2010
Fragments arranged by size:
2010 915 714 607 364 262 191
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5063
SphI G_CATG'C
Cuts at: 2116 2616 2116
Size: 500 4563
StuI AGG'CCT
Cuts at: 1198 1648 1198
Size: 450 4613
StyI C'CwwG_G
Cuts at: 107 1933 1966 2197 2551 107
Size: 1826 33 231 354 2619
Fragments arranged by size:
2619 1826 354 231 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 718 2646 2779 3238 3447 718
Size: 1928 133 459 209 2334
Fragments arranged by size:
2334 1928 459 209 133
TatI w'GTAC_w
Cuts at: 157 466 725 751 1943 4578 157
Size: 309 259 26 1192 2635 642
Fragments arranged by size:
2635 1192 642 309 259 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1836 1868 4580 4612 1836
Size: 32 2712 32 2287
Fragments arranged by size:
2712 2287 32 32
Tth111I GACn'n_nGTC
Cuts at: 2860 2860
Size: 5063
XbaI T'CTAG_A
Cuts at: 492 2094 492
Size: 1602 3461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2558 2558
Size: 5063
XmaI C'CCGG_G
Cuts at: 929 2083 929
Size: 1154 3909
XmnI GAAnn'nnTTC
Cuts at: 912 1715 912
Size: 803 4260
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PmeI PpiI PpuMI PsiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI BbvCI BcgI BfrBI BmgBI BmtI BseRI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI MfeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI