Restriction map for
pF1KB9400
(Circular) (Six-base) MAPSORT of: pF1KB9400.seq Check: 6714 from: 1 to: 5636
pF1KB9400 5636 bp
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 252 252
Size: 5636
AccI GT'mk_AC
Cuts at: 1355 2453 2674 1355
Size: 1098 221 4317
Fragments arranged by size:
4317 1098 221
Acc65I G'GTAC_C
Cuts at: 404 2103 2652 404
Size: 1699 549 3388
Fragments arranged by size:
3388 1699 549
AclI AA'CG_TT
Cuts at: 5476 5476
Size: 5636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2382 3461 3893 4640 4908 1129
Size: 1253 1079 432 747 268 1857
Fragments arranged by size:
1857 1253 1079 747 432 268
AfeI AGC'GCT
Cuts at: 2239 2239
Size: 5636
AflIII A'CryG_T
Cuts at: 2988 4092 5069 2988
Size: 1104 977 3555
Fragments arranged by size:
3555 1104 977
AgeI A'CCGG_T
Cuts at: 4024 4024
Size: 5636
AleI CACnn'nnGTG
Cuts at: 916 1039 1777 1891 916
Size: 123 738 114 4661
Fragments arranged by size:
4661 738 123 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2485 2485
Size: 5636
AlwNI CAG_nnn'CTG
Cuts at: 789 1317 1607 2941 4508 789
Size: 528 290 1334 1567 1917
Fragments arranged by size:
1917 1567 1334 528 290
ApaI G_GGCC'C
Cuts at: 1020 1020
Size: 5636
ApaLI G'TGCA_C
Cuts at: 2265 4406 2265
Size: 2141 3495
ApoI r'AATT_y
Cuts at: 2640 4989 5245 2640
Size: 2349 256 3031
Fragments arranged by size:
3031 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5636
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5636
AvaI C'yCGr_G
Cuts at: 277 1045 1133 2656 277
Size: 768 88 1523 3257
Fragments arranged by size:
3257 1523 768 88
AvrII C'CTAG_G
Cuts at: 1153 1153
Size: 5636
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2087 2120 2087
Size: 33 5603
BamHI G'GATC_C
Cuts at: 1049 2661 1049
Size: 1612 4024
BanI G'GyrC_C
Cuts at: 404 597 1041 1581 1619 1740 1877 2103 2652
Size: 193 444 540 38 121 137 226 549
Cuts at: 2652 3066 3314 3349 404
Size: 414 248 35 2691
Fragments arranged by size:
2691 549 540 444 414 248 226 193
137 121 38 35
BanII G_rGCy'C
Cuts at: 494 589 1020 1174 1939 2650 3680 494
Size: 95 431 154 765 711 1030 2450
Fragments arranged by size:
2450 1030 765 711 431 154 95
BbeI G_GCGC'C
Cuts at: 3070 3318 3070
Size: 248 5388
BbsI GAAGACnn'nnnn_
Cuts at: 182 182
Size: 5636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2499 2533 90
Size: 34 2375 34 3193
Fragments arranged by size:
3193 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 662 3534 4295 5636 662
Size: 2872 761 1341 662
Fragments arranged by size:
2872 1341 761 662
BclI T'GATC_A
Cuts at: 2159 3156 2159
Size: 997 4639
BglI GCCn_nnn'nGGC
Cuts at: 134 1169 1967 134
Size: 1035 798 3803
Fragments arranged by size:
3803 1035 798
BglII A'GATC_T
Cuts at: 3151 5188 3151
Size: 2037 3599
BlpI GC'TnA_GC
Cuts at: 583 634 2166 2497 2748 583
Size: 51 1532 331 251 3471
Fragments arranged by size:
3471 1532 331 251 51
Bme1580I G_kGCm'C
Cuts at: 602 1020 1046 1622 1670 1743 2269 3261 3354
Size: 418 26 576 48 73 526 992 93
Cuts at: 3354 4410 602
Size: 1056 1828
Fragments arranged by size:
1828 1056 992 576 526 418 93 73
48 26
BmrI ACTGGGnnnn_n'
Cuts at: 1046 2848 2929 3264 5408 1046
Size: 1802 81 335 2144 1274
Fragments arranged by size:
2144 1802 1274 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 238 523 1459 1821 2295 4003 238
Size: 285 936 362 474 1708 1871
Fragments arranged by size:
1871 1708 936 474 362 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1008 1352 1508 811
Size: 197 344 156 4939
Fragments arranged by size:
4939 344 197 156
Bpu10I CC'TnA_GC
Cuts at: 2506 2506
Size: 5636
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2281 2812 3691 4183 4481 2281
Size: 531 879 492 298 3436
Fragments arranged by size:
3436 879 531 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 494 691 1526 1540 2266 5340 494
Size: 197 835 14 726 3074 790
Fragments arranged by size:
3074 835 790 726 197 14
BsaAI yAC'GTr
Cuts at: 2547 2989 2547
Size: 442 5194
BsaBI GATnn'nnATC
Cuts at: 6 3150 5187 6
Size: 3144 2037 455
Fragments arranged by size:
3144 2037 455
BsaHI Gr'CG_yC
Cuts at: 3067 3315 4017 5513 3067
Size: 248 702 1496 3190
Fragments arranged by size:
3190 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2825 3346 4024 4298 4445 4856 2825
Size: 521 678 274 147 411 3605
Fragments arranged by size:
3605 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1156 1186 1506 1536 1156
Size: 30 320 30 5256
Fragments arranged by size:
5256 320 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 2308 373
Size: 1935 3701
BseYI C'CCAG_C
Cuts at: 161 1071 1678 1932 2174 2491 3061 3819 4396
Size: 910 607 254 242 317 570 758 577
Cuts at: 4396 4892 161
Size: 496 905
Fragments arranged by size:
910 905 758 607 577 570 496 317
254 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 802 1200 102
Size: 700 398 4538
Fragments arranged by size:
4538 700 398
BsiEI CG_ry'CG
Cuts at: 104 3178 3224 4432 104
Size: 3074 46 1208 1308
Fragments arranged by size:
3074 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 464 494 1802 2269 2585 2650 3428 3618 4410
Size: 30 1308 467 316 65 778 190 792
Cuts at: 4410 464
Size: 1690
Fragments arranged by size:
1690 1308 792 778 467 316 190 65
30
BsmI GAATG_Cn'
Cuts at: 1210 1210
Size: 5636
BsmBI CGTCTCn'nnnn_
Cuts at: 622 622
Size: 5636
Bsp1286I G_dGCh'C
Cuts at: 464 494 589 602 1020 1046 1174 1622 1670
Size: 30 95 13 418 26 128 448 48
Cuts at: 1670 1743 1802 1939 2269 2585 2650 3261 3354
Size: 73 59 137 330 316 65 611 93
Cuts at: 3354 3428 3618 3680 4410 464
Size: 74 190 62 730 1690
Fragments arranged by size:
1690 730 611 448 418 330 316 190
137 128 95 93 74 73 65 62
59 48 30 26 13
BspEI T'CCGG_A
Cuts at: 4856 4856
Size: 5636
BspHI T'CATG_A
Cuts at: 4812 4812
Size: 5636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 252 1768 1939 2477 2686 2900 3202 3583 252
Size: 1516 171 538 209 214 302 381 2305
Fragments arranged by size:
2305 1516 538 381 302 214 209 171
BsrBI CCG'CTC
Cuts at: 50 3928 3982 5466 5631 50
Size: 3878 54 1484 165 55
Fragments arranged by size:
3878 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 821 3548 821
Size: 2727 2909
BsrFI r'CCGG_y
Cuts at: 935 3634 3815 4024 935
Size: 2699 181 209 2547
Fragments arranged by size:
2699 2547 209 181
BssSI C'ACGA_G
Cuts at: 2610 3907 4265 2610
Size: 1297 358 3981
Fragments arranged by size:
3981 1297 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1205 3013 1205
Size: 1808 3828
BstBI TT'CG_AA
Cuts at: 3997 3997
Size: 5636
BstEII G'GTnAC_C
Cuts at: 282 282
Size: 5636
BstXI CCAn_nnnn'nTGG
Cuts at: 805 918 1060 1723 2143 2283 2487 805
Size: 113 142 663 420 140 204 3954
Fragments arranged by size:
3954 663 420 204 142 140 113
BstYI r'GATC_y
Cuts at: 1 737 815 1049 1379 1437 2661 3128 3151
Size: 736 78 234 330 58 1224 467 23
Cuts at: 3151 3486 3732 4744 4830 4842 5188 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1224 1012 736 467 449 346 335 330
246 234 86 78 58 23 12
Bsu36I CC'TnA_GG
Cuts at: 1745 1745
Size: 5636
BtgI C'CryG_G
Cuts at: 374 701 965 1299 2136 2441 374
Size: 327 264 334 837 305 3569
Fragments arranged by size:
3569 837 334 327 305 264
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 878 3586 3767 3930 878
Size: 2708 181 163 2584
Fragments arranged by size:
2708 2584 181 163
BtsI GCAGTG_nn'
Cuts at: 539 762 883 1647 2215 2879 539
Size: 223 121 764 568 664 3296
Fragments arranged by size:
3296 764 664 568 223 121
DraI TTT'AAA
Cuts at: 2635 2635
Size: 5636
DraIII CAC_nnn'GTG
Cuts at: 1389 1389
Size: 5636
DrdI GACnn_nn'nnGTC
Cuts at: 3342 4200 3342
Size: 858 4778
EaeI y'GGCC_r
Cuts at: 617 944 1079 2444 2477 3221 3395 3786 3813
Size: 327 135 1365 33 744 174 391 27
Cuts at: 3813 5158 617
Size: 1345 1095
Fragments arranged by size:
1365 1345 1095 744 391 327 174 135
33 27
EagI C'GGCC_G
Cuts at: 3221 3221
Size: 5636
EarI CTCTTCn'nnn_
Cuts at: 337 1084 1727 2413 3659 3869 337
Size: 747 643 686 1246 210 2104
Fragments arranged by size:
2104 1246 747 686 643 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3844 4154 4300 5446 3844
Size: 310 146 1146 4034
Fragments arranged by size:
4034 1146 310 146
EcoICRI GAG'CTC
Cuts at: 492 2648 492
Size: 2156 3480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1008 1129 1352 1508 2382 3461 3893 4640
Size: 197 121 223 156 874 1079 432 747
Cuts at: 4640 4908 811
Size: 268 1539
Fragments arranged by size:
1539 1079 874 747 432 268 223 197
156 121
EcoNI CCTnn'n_nnAGG
Cuts at: 334 898 2353 334
Size: 564 1455 3617
Fragments arranged by size:
3617 1455 564
EcoO109I rG'GnC_Cy
Cuts at: 1017 1149 1682 2013 2775 1017
Size: 132 533 331 762 3878
Fragments arranged by size:
3878 762 533 331 132
EcoRI G'AATT_C
Cuts at: 2640 2640
Size: 5636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 520 1509 3655 520
Size: 989 2146 2501
Fragments arranged by size:
2501 2146 989
FspI TGC'GCA
Cuts at: 667 3417 667
Size: 2750 2886
HaeII r_GCGC'y
Cuts at: 2241 2619 3070 3318 4340 5319 2241
Size: 378 451 248 1022 979 2558
Fragments arranged by size:
2558 1022 979 451 378 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3478 3508 3510 3540 3478
Size: 30 2 30 5574
Fragments arranged by size:
5574 30 30 2
HincII GTy'rAC
Cuts at: 2675 2675
Size: 5636
HindIII A'AGCT_T
Cuts at: 1211 1211
Size: 5636
KasI G'GCGC_C
Cuts at: 3066 3314 3066
Size: 248 5388
KpnI G_GTAC'C
Cuts at: 408 2107 2656 408
Size: 1699 549 3388
Fragments arranged by size:
3388 1699 549
MluI A'CGCG_T
Cuts at: 5069 5069
Size: 5636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2094 4307 4491 2094
Size: 2213 184 3239
Fragments arranged by size:
3239 2213 184
MscI TGG'CCA
Cuts at: 946 1081 3397 946
Size: 135 2316 3185
Fragments arranged by size:
3185 2316 135
MslI CAynn'nnrTG
Cuts at: 916 1039 1063 1777 1891 3625 3752 916
Size: 123 24 714 114 1734 127 2800
Fragments arranged by size:
2800 1734 714 127 123 114 24
MspA1I CmG'CkG
Cuts at: 411 2170 2178 2513 2747 3061 3421 4434 4679
Size: 1759 8 335 234 314 360 1013 245
Cuts at: 4679 411
Size: 1368
Fragments arranged by size:
1759 1368 1013 360 335 314 245 234
8
NaeI GCC'GGC
Cuts at: 937 3817 937
Size: 2880 2756
NarI GG'CG_CC
Cuts at: 3067 3315 3067
Size: 248 5388
NcoI C'CATG_G
Cuts at: 701 1299 701
Size: 598 5038
NgoMIV G'CCGG_C
Cuts at: 935 3815 935
Size: 2880 2756
NspI r_CATG'y
Cuts at: 2689 3189 4096 2689
Size: 500 907 4229
Fragments arranged by size:
4229 907 500
PasI CC'CwG_GG
Cuts at: 399 2283 399
Size: 1884 3752
PciI A'CATG_T
Cuts at: 4092 4092
Size: 5636
PflMI CCAn_nnn'nTGG
Cuts at: 1317 1629 1317
Size: 312 5324
PfoI T'CCnGG_A
Cuts at: 2422 2422
Size: 5636
PmeI GTTT'AAAC
Cuts at: 2635 2635
Size: 5636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4775 4807 4775
Size: 32 5604
PpuMI rG'GwC_Cy
Cuts at: 1682 1682
Size: 5636
PspOMI G'GGCC_C
Cuts at: 1016 1016
Size: 5636
PstI C_TGCA'G
Cuts at: 143 263 719 1201 2344 2683 3368 143
Size: 120 456 482 1143 339 685 2411
Fragments arranged by size:
2411 1143 685 482 456 339 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5636
PvuII CAG'CTG
Cuts at: 411 2178 3061 3421 411
Size: 1767 883 360 2626
Fragments arranged by size:
2626 1767 883 360
RsrII CG'GwC_CG
Cuts at: 3831 3831
Size: 5636
SacI G_AGCT'C
Cuts at: 494 2650 494
Size: 2156 3480
SalI G'TCGA_C
Cuts at: 2673 2673
Size: 5636
SapI GCTCTTCn'nnn_
Cuts at: 2413 3659 3869 2413
Size: 1246 210 4180
Fragments arranged by size:
4180 1246 210
SbfI CC_TGCA'GG
Cuts at: 2683 2683
Size: 5636
ScaI AGT'ACT
Cuts at: 5153 5153
Size: 5636
SexAI A'CCwGG_T
Cuts at: 689 1577 689
Size: 888 4748
SfcI C'TryA_G
Cuts at: 33 139 259 715 1197 1707 2340 2679 3364
Size: 106 120 456 482 510 633 339 685
Cuts at: 3364 4357 4548 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 633 510 482 456 339
191 120 106
SfoI GGC'GCC
Cuts at: 3068 3316 3068
Size: 248 5388
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5636
SmaI CCC'GGG
Cuts at: 1047 1135 2658 1047
Size: 88 1523 4025
Fragments arranged by size:
4025 1523 88
SmlI C'TyrA_G
Cuts at: 2260 2791 3706 4198 4460 2260
Size: 531 915 492 262 3436
Fragments arranged by size:
3436 915 531 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5636
SphI G_CATG'C
Cuts at: 2689 3189 2689
Size: 500 5136
SrfI GCCC'GGGC
Cuts at: 1135 1135
Size: 5636
StuI AGG'CCT
Cuts at: 233 1158 1992 2593 233
Size: 925 834 601 3276
Fragments arranged by size:
3276 925 834 601
StyI C'CwwG_G
Cuts at: 495 701 1153 1299 2025 2099 2770 3124 495
Size: 206 452 146 726 74 671 354 3007
Fragments arranged by size:
3007 726 671 452 354 206 146 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1800 3219 3352 3811 4020 1800
Size: 1419 133 459 209 3416
Fragments arranged by size:
3416 1419 459 209 133
TatI w'GTAC_w
Cuts at: 5151 5151
Size: 5636
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1156 1188 1506 1538 5153 5185 1156
Size: 32 318 32 3615 32 1607
Fragments arranged by size:
3615 1607 318 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3433 3433
Size: 5636
XbaI T'CTAG_A
Cuts at: 2667 2667
Size: 5636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 396 939 3131 396
Size: 543 2192 2901
Fragments arranged by size:
2901 2192 543
XmaI C'CCGG_G
Cuts at: 1045 1133 2656 1045
Size: 88 1523 4025
Fragments arranged by size:
4025 1523 88
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI BbvCI BfrBI BmgBI
BmtI BsiWI BsrGI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SspI SwaI XhoI XmnI
ZraI