Restriction map for
pF1KB9403
(Circular) (Six-base) MAPSORT of: pF1KB9403.seq Check: 1072 from: 1 to: 6929
pF1KB9403 6929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3885 3885
Size: 6929
AatII G_ACGT'C
Cuts at: 2186 2186
Size: 6929
AccI GT'mk_AC
Cuts at: 3967 3967
Size: 6929
Acc65I G'GTAC_C
Cuts at: 1340 3140 3716 3945 1340
Size: 1800 576 229 4324
Fragments arranged by size:
4324 1800 576 229
AclI AA'CG_TT
Cuts at: 6769 6769
Size: 6929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 876 994 1642 2104 2170 2464 2491 2577 2806
Size: 118 648 462 66 294 27 86 229
Cuts at: 2806 4754 5186 5539 6201 876
Size: 1948 432 353 662 1604
Fragments arranged by size:
1948 1604 662 648 462 432 353 294
229 118 86 66 27
AfeI AGC'GCT
Cuts at: 827 2037 3661 827
Size: 1210 1624 4095
Fragments arranged by size:
4095 1624 1210
AflIII A'CryG_T
Cuts at: 4281 6081 6362 4281
Size: 1800 281 4848
Fragments arranged by size:
4848 1800 281
AleI CACnn'nnGTG
Cuts at: 2965 2965
Size: 6929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 754 1792 754
Size: 1038 5891
AlwNI CAG_nnn'CTG
Cuts at: 957 2481 3150 3501 3916 4234 5672 957
Size: 1524 669 351 415 318 1438 2214
Fragments arranged by size:
2214 1524 1438 669 415 351 318
ApaI G_GGCC'C
Cuts at: 1431 3130 1431
Size: 1699 5230
ApaLI G'TGCA_C
Cuts at: 808 5767 808
Size: 4959 1970
ApoI r'AATT_y
Cuts at: 394 2597 3933 6282 6538 394
Size: 2203 1336 2349 256 785
Fragments arranged by size:
2349 2203 1336 785 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6929
AvaI C'yCGr_G
Cuts at: 249 724 1548 3949 249
Size: 475 824 2401 3229
Fragments arranged by size:
3229 2401 824 475
AvrII C'CTAG_G
Cuts at: 3114 3114
Size: 6929
BamHI G'GATC_C
Cuts at: 3954 3954
Size: 6929
BanI G'GyrC_C
Cuts at: 310 1340 1660 1785 2637 3140 3716 3945 4359
Size: 1030 320 125 852 503 576 229 414
Cuts at: 4359 4607 4642 310
Size: 248 35 2597
Fragments arranged by size:
2597 1030 852 576 503 414 320 248
229 125 35
BanII G_rGCy'C
Cuts at: 242 856 1431 1558 2250 2399 3130 3167 3943
Size: 614 575 127 692 149 731 37 776
Cuts at: 3943 4973 242
Size: 1030 2198
Fragments arranged by size:
2198 1030 776 731 692 614 575 149
127 37
BbeI G_GCGC'C
Cuts at: 314 4363 4611 314
Size: 4049 248 2632
Fragments arranged by size:
4049 2632 248
BbsI GAAGACnn'nnnn_
Cuts at: 174 1444 1842 2240 174
Size: 1270 398 398 4863
Fragments arranged by size:
4863 1270 398 398
BbvCI CC'TCA_GC
Cuts at: 2800 3088 3436 2800
Size: 288 348 6293
Fragments arranged by size:
6293 348 288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 201 235 1201 1235 3729 3763 201
Size: 34 966 34 2494 34 3367
Fragments arranged by size:
3367 2494 966 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4827 5883 6929 4827
Size: 1056 1046 4827
Fragments arranged by size:
4827 1056 1046
BclI T'GATC_A
Cuts at: 4449 4449
Size: 6929
BglI GCCn_nnn'nGGC
Cuts at: 112 173 1253 3874 112
Size: 61 1080 2621 3167
Fragments arranged by size:
3167 2621 1080 61
BglII A'GATC_T
Cuts at: 2538 3456 4444 6481 2538
Size: 918 988 2037 2986
Fragments arranged by size:
2986 2037 988 918
BlpI GC'TnA_GC
Cuts at: 2393 4041 2393
Size: 1648 5281
Bme1580I G_kGCm'C
Cuts at: 812 1431 1790 1956 3130 4554 4647 5771 812
Size: 619 359 166 1174 1424 93 1124 1970
Fragments arranged by size:
1970 1424 1174 1124 619 359 166 93
BmgBI CAC'GTC
Cuts at: 3179 3179
Size: 6929
BmrI ACTGGGnnnn_n'
Cuts at: 1428 2036 2699 3403 4141 4222 4557 6701 1428
Size: 608 663 704 738 81 335 2144 1656
Fragments arranged by size:
2144 1656 738 704 663 608 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 5296 2413
Size: 2883 4046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 523 1183 2062 2116 2206 2305 2385 2758
Size: 158 660 879 54 90 99 80 373
Cuts at: 2758 3172 3361 3473 3610 365
Size: 414 189 112 137 3684
Fragments arranged by size:
3684 879 660 414 373 189 158 137
112 99 90 80 54
Bpu10I CC'TnA_GC
Cuts at: 143 2800 3074 3088 3436 3656 143
Size: 2657 274 14 348 220 3416
Fragments arranged by size:
3416 2657 348 274 220 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2119 2962 3581 3718 4105 4984 5698 5996 2119
Size: 843 619 137 387 879 714 298 3052
Fragments arranged by size:
3052 879 843 714 619 387 298 137
BsaI GGTCTCn'nnnn_
Cuts at: 560 1199 2312 2999 3238 6633 560
Size: 639 1113 687 239 3395 856
Fragments arranged by size:
3395 1113 856 687 639 239
BsaAI yAC'GTr
Cuts at: 750 2772 3666 4282 750
Size: 2022 894 616 3397
Fragments arranged by size:
3397 2022 894 616
BsaBI GATnn'nnATC
Cuts at: 6 4443 6480 6
Size: 4437 2037 455
Fragments arranged by size:
4437 2037 455
BsaHI Gr'CG_yC
Cuts at: 311 2183 4360 4608 5310 6806 311
Size: 1872 2177 248 702 1496 434
Fragments arranged by size:
2177 1872 1496 702 434 248
BsaWI w'CCGG_w
Cuts at: 1178 1902 2622 3224 4118 4639 5317 5728 5875
Size: 724 720 602 894 521 678 411 147
Cuts at: 5875 6149 1178
Size: 274 1958
Fragments arranged by size:
1958 894 724 720 678 602 521 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1981 2011 3040 3070 3192 3222 3277 3307 1981
Size: 30 1029 30 122 30 55 30 5603
Fragments arranged by size:
5603 1029 122 55 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 304 319 1228 1548 1926 1929 3346 3553 3715
Size: 15 909 320 378 3 1417 207 162
Cuts at: 3715 304
Size: 3518
Fragments arranged by size:
3518 1417 909 378 320 207 162 15
3
BseYI C'CCAG_C
Cuts at: 284 484 742 1932 2327 2756 2795 3015 3104
Size: 200 258 1190 395 429 39 220 89
Cuts at: 3104 3691 3881 4354 5112 5777 6185 284
Size: 587 190 473 758 665 408 1028
Fragments arranged by size:
1190 1028 758 665 587 473 429 408
395 258 220 200 190 89 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 604 932 3347 3580 192
Size: 412 328 2415 233 3541
Fragments arranged by size:
3541 2415 412 328 233
BsiEI CG_ry'CG
Cuts at: 104 125 967 3058 3445 4471 4517 5747 104
Size: 21 842 2091 387 1026 46 1230 1286
Fragments arranged by size:
2091 1286 1230 1026 842 387 46 21
BsiHKAI G_wGCw'C
Cuts at: 812 1418 1537 2399 3943 4721 4911 5771 812
Size: 606 119 862 1544 778 190 860 1970
Fragments arranged by size:
1970 1544 862 860 778 606 190 119
BsmI GAATG_Cn'
Cuts at: 349 1298 349
Size: 949 5980
Bsp1286I G_dGCh'C
Cuts at: 242 812 856 1418 1431 1537 1558 1790 1956
Size: 570 44 562 13 106 21 232 166
Cuts at: 1956 2250 2399 3130 3167 3943 4554 4647 4721
Size: 294 149 731 37 776 611 93 74
Cuts at: 4721 4911 4973 5771 242
Size: 190 62 798 1400
Fragments arranged by size:
1400 798 776 731 611 570 562 294
232 190 166 149 106 93 74 62
44 37 21 13
BspEI T'CCGG_A
Cuts at: 2622 3224 2622
Size: 602 6327
BspHI T'CATG_A
Cuts at: 1038 5361 1038
Size: 4323 2606
BspMI ACCTGCnnnn'nnnn_
Cuts at: 281 1586 3128 3500 3885 3979 4193 4495 4876
Size: 1305 1542 372 385 94 214 302 381
Cuts at: 4876 281
Size: 2334
Fragments arranged by size:
2334 1542 1305 385 381 372 302 214
94
BsrBI CCG'CTC
Cuts at: 50 266 999 1793 3447 5221 5275 6759 6924
Size: 216 733 794 1654 1774 54 1484 165
Cuts at: 6924 50
Size: 55
Fragments arranged by size:
1774 1654 1484 794 733 216 165 55
54
BsrDI GCAATG_nn'
Cuts at: 3203 4841 3203
Size: 1638 5291
BsrFI r'CCGG_y
Cuts at: 3440 4927 5108 3440
Size: 1487 181 5261
Fragments arranged by size:
5261 1487 181
BssSI C'ACGA_G
Cuts at: 5200 5908 5200
Size: 708 6221
BstAPI GCAn_nnn'nTGC
Cuts at: 2361 2481 3567 4306 2361
Size: 120 1086 739 4984
Fragments arranged by size:
4984 1086 739 120
BstBI TT'CG_AA
Cuts at: 5290 5290
Size: 6929
BstXI CCAn_nnnn'nTGG
Cuts at: 556 1486 2418 556
Size: 930 932 5067
Fragments arranged by size:
5067 932 930
BstYI r'GATC_y
Cuts at: 1 2106 2538 3456 3675 3734 3954 4421 4444
Size: 2105 432 918 219 59 220 467 23
Cuts at: 4444 4779 5025 5331 5343 5429 6481 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
2105 1052 918 467 449 432 335 306
246 220 219 86 59 23 12
Bsu36I CC'TnA_GG
Cuts at: 770 3783 770
Size: 3013 3916
BtgI C'CryG_G
Cuts at: 107 279 479 599 2009 107
Size: 172 200 120 1410 5027
Fragments arranged by size:
5027 1410 200 172 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4879 5060 5223 4879
Size: 181 163 6585
Fragments arranged by size:
6585 181 163
BtsI GCAGTG_nn'
Cuts at: 2561 3028 3917 4172 2561
Size: 467 889 255 5318
Fragments arranged by size:
5318 889 467 255
DraI TTT'AAA
Cuts at: 3928 3928
Size: 6929
DraIII CAC_nnn'GTG
Cuts at: 1667 1667
Size: 6929
DrdI GACnn_nn'nnGTC
Cuts at: 2182 4635 5979 2182
Size: 2453 1344 3132
Fragments arranged by size:
3132 2453 1344
EaeI y'GGCC_r
Cuts at: 174 1047 1089 1488 1864 3442 4514 4688 5079
Size: 873 42 399 376 1578 1072 174 391
Cuts at: 5079 5106 6451 174
Size: 27 1345 652
Fragments arranged by size:
1578 1345 1072 873 652 399 391 376
174 42 27
EagI C'GGCC_G
Cuts at: 3442 4514 3442
Size: 1072 5857
EarI CTCTTCn'nnn_
Cuts at: 404 556 970 1529 1631 2493 2755 3677 4952
Size: 152 414 559 102 862 262 922 1275
Cuts at: 4952 5162 404
Size: 210 2171
Fragments arranged by size:
2171 1275 922 862 559 414 262 210
152 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5137 5879 6025 6739 5137
Size: 742 146 714 5327
Fragments arranged by size:
5327 742 714 146
EcoICRI GAG'CTC
Cuts at: 2397 3941 2397
Size: 1544 5385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 523 876 994 1183 1642 2062 2104 2116
Size: 158 353 118 189 459 420 42 12
Cuts at: 2116 2170 2206 2305 2385 2464 2491 2577 2758
Size: 54 36 99 80 79 27 86 181
Cuts at: 2758 2806 3172 3361 3473 3610 4754 5186 5539
Size: 48 366 189 112 137 1144 432 353
Cuts at: 5539 6201 365
Size: 662 1093
Fragments arranged by size:
1144 1093 662 459 432 420 366 353
353 189 189 181 158 137 118 112
99 86 80 79 54 48 42 36
27 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1832 2134 1832
Size: 302 6627
EcoO109I rG'GnC_Cy
Cuts at: 881 1057 1155 1427 1483 1837 2127 2217 2373
Size: 176 98 272 56 354 290 90 156
Cuts at: 2373 2876 2973 3127 3366 4068 881
Size: 503 97 154 239 702 3742
Fragments arranged by size:
3742 702 503 354 290 272 239 176
156 154 98 97 90 56
EcoRI G'AATT_C
Cuts at: 394 2597 3933 394
Size: 2203 1336 3390
Fragments arranged by size:
3390 2203 1336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3276 3297 4948 3276
Size: 21 1651 5257
Fragments arranged by size:
5257 1651 21
FspI TGC'GCA
Cuts at: 1601 4710 1601
Size: 3109 3820
HaeII r_GCGC'y
Cuts at: 314 829 1644 1695 1726 2039 3291 3663 4363
Size: 515 815 51 31 313 1252 372 700
Cuts at: 4363 4611 5841 6612 314
Size: 248 1230 771 631
Fragments arranged by size:
1252 1230 815 771 700 631 515 372
313 248 51 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 509 541 815 847 1062 1094 1458
Size: 32 341 32 274 32 215 32 364
Cuts at: 1458 1490 1981 2013 3040 3072 3448 3480 3631
Size: 32 491 32 1027 32 376 32 151
Cuts at: 3631 3663 4771 4801 4803 4833 136
Size: 32 1108 30 2 30 2232
Fragments arranged by size:
2232 1108 1027 491 376 364 341 274
215 151 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 246 1107 3968 246
Size: 861 2861 3207
Fragments arranged by size:
3207 2861 861
KasI G'GCGC_C
Cuts at: 310 4359 4607 310
Size: 4049 248 2632
Fragments arranged by size:
4049 2632 248
KpnI G_GTAC'C
Cuts at: 1344 3144 3720 3949 1344
Size: 1800 576 229 4324
Fragments arranged by size:
4324 1800 576 229
MluI A'CGCG_T
Cuts at: 6362 6362
Size: 6929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2429 3563 5688 5872 2429
Size: 1134 2125 184 3486
Fragments arranged by size:
3486 2125 1134 184
MscI TGG'CCA
Cuts at: 1091 1490 4690 1091
Size: 399 3200 3330
Fragments arranged by size:
3330 3200 399
MslI CAynn'nnrTG
Cuts at: 817 1043 1655 1801 2416 2965 4918 5045 817
Size: 226 612 146 615 549 1953 127 2701
Fragments arranged by size:
2701 1953 615 612 549 226 146 127
MspA1I CmG'CkG
Cuts at: 206 281 850 1347 2082 2469 2760 2799 3092
Size: 75 569 497 735 387 291 39 293
Cuts at: 3092 3339 3825 4040 4354 4714 5498 5743 206
Size: 247 486 215 314 360 784 245 1392
Fragments arranged by size:
1392 784 735 569 497 486 387 360
314 293 291 247 245 215 75 39
NaeI GCC'GGC
Cuts at: 3442 5110 3442
Size: 1668 5261
NarI GG'CG_CC
Cuts at: 311 4360 4608 311
Size: 4049 248 2632
Fragments arranged by size:
4049 2632 248
NcoI C'CATG_G
Cuts at: 107 2009 107
Size: 1902 5027
NdeI CA'TA_TG
Cuts at: 425 1308 425
Size: 883 6046
NgoMIV G'CCGG_C
Cuts at: 3440 5108 3440
Size: 1668 5261
NspI r_CATG'y
Cuts at: 1779 3765 3982 4482 6085 1779
Size: 1986 217 500 1603 2623
Fragments arranged by size:
2623 1986 1603 500 217
PciI A'CATG_T
Cuts at: 6081 6081
Size: 6929
PflMI CCAn_nnn'nTGG
Cuts at: 2229 2334 2229
Size: 105 6824
PfoI T'CCnGG_A
Cuts at: 2222 2513 2222
Size: 291 6638
PmeI GTTT'AAAC
Cuts at: 3928 3928
Size: 6929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5375 5407 5375
Size: 32 6897
PpuMI rG'GwC_Cy
Cuts at: 881 1057 1155 1483 1837 2127 2217 2373 881
Size: 176 98 328 354 290 90 156 5437
Fragments arranged by size:
5437 354 328 290 176 156 98 90
PspOMI G'GGCC_C
Cuts at: 1427 3126 1427
Size: 1699 5230
PstI C_TGCA'G
Cuts at: 2369 2486 2568 3497 3710 3830 3976 4661 2369
Size: 117 82 929 213 120 146 685 4637
Fragments arranged by size:
4637 929 685 213 146 120 117 82
PvuI CG_AT'CG
Cuts at: 104 967 104
Size: 863 6066
PvuII CAG'CTG
Cuts at: 2082 2469 2760 2799 3339 3825 4354 4714 2082
Size: 387 291 39 540 486 529 360 4297
Fragments arranged by size:
4297 540 529 486 387 360 291 39
RsrII CG'GwC_CG
Cuts at: 5124 5124
Size: 6929
SacI G_AGCT'C
Cuts at: 2399 3943 2399
Size: 1544 5385
SacII CC_GC'GG
Cuts at: 282 282
Size: 6929
SalI G'TCGA_C
Cuts at: 3966 3966
Size: 6929
SanDI GG'GwC_CC
Cuts at: 1057 1155 1057
Size: 98 6831
SapI GCTCTTCn'nnn_
Cuts at: 404 1631 2493 3677 4952 5162 404
Size: 1227 862 1184 1275 210 2171
Fragments arranged by size:
2171 1275 1227 1184 862 210
SbfI CC_TGCA'GG
Cuts at: 2486 3976 2486
Size: 1490 5439
ScaI AGT'ACT
Cuts at: 6446 6446
Size: 6929
SfcI C'TryA_G
Cuts at: 33 2365 2482 2564 2895 3493 3706 3772 3826
Size: 2332 117 82 331 598 213 66 54
Cuts at: 3826 3972 4657 5625 5816 33
Size: 146 685 968 191 1146
Fragments arranged by size:
2332 1146 968 685 598 331 213 191
146 117 82 66 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 1253 3874 1253
Size: 2621 4308
SfoI GGC'GCC
Cuts at: 312 4361 4609 312
Size: 4049 248 2632
Fragments arranged by size:
4049 2632 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6929
SmaI CCC'GGG
Cuts at: 3951 3951
Size: 6929
SmlI C'TyrA_G
Cuts at: 2134 2977 3596 3697 4084 4999 5713 5975 2134
Size: 843 619 101 387 915 714 262 3088
Fragments arranged by size:
3088 915 843 714 619 387 262 101
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6929
SphI G_CATG'C
Cuts at: 1779 3982 4482 1779
Size: 2203 500 4226
Fragments arranged by size:
4226 2203 500
SspI AAT'ATT
Cuts at: 793 793
Size: 6929
StuI AGG'CCT
Cuts at: 2570 3877 2570
Size: 1307 5622
StyI C'CwwG_G
Cuts at: 107 1457 1734 1843 1945 2009 3114 3281 3399
Size: 1350 277 109 102 64 1105 167 118
Cuts at: 3399 4063 4417 107
Size: 664 354 2619
Fragments arranged by size:
2619 1350 1105 664 354 277 167 118
109 102 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 919 1622 2006 2804 3705 4512 4645 5104 5313
Size: 703 384 798 901 807 133 459 209
Cuts at: 5313 919
Size: 2535
Fragments arranged by size:
2535 901 807 798 703 459 384 209
133
TatI w'GTAC_w
Cuts at: 3293 6444 3293
Size: 3151 3778
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1015 1047 3192 3224 6446 6478 1015
Size: 32 2145 32 3222 32 1466
Fragments arranged by size:
3222 2145 1466 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1102 2215 2377 4726 1102
Size: 1113 162 2349 3305
Fragments arranged by size:
3305 2349 1113 162
XbaI T'CTAG_A
Cuts at: 3960 3960
Size: 6929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 597 2007 4424 597
Size: 1410 2417 3102
Fragments arranged by size:
3102 2417 1410
XmaI C'CCGG_G
Cuts at: 3949 3949
Size: 6929
XmnI GAAnn'nnTTC
Cuts at: 398 398
Size: 6929
ZraI GAC'GTC
Cuts at: 2184 2184
Size: 6929
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI AscI BaeI BfrBI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
PsrI SexAI SgrAI SnaBI SrfI SwaI XhoI