Restriction map for
pF1KB9405
(Circular) (Six-base) MAPSORT of: pF1KB9405.seq Check: 399 from: 1 to: 4625
pF1KB9405 4625 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1663 1663
Size: 4625
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 2450 2882 3629 3897 776
Size: 1674 432 747 268 1504
Fragments arranged by size:
1674 1504 747 432 268
AflIII A'CryG_T
Cuts at: 1977 3081 4058 1977
Size: 1104 977 2544
Fragments arranged by size:
2544 1104 977
AgeI A'CCGG_T
Cuts at: 3013 3013
Size: 4625
AhdI GACnn_n'nnGTC
Cuts at: 1566 1566
Size: 4625
AlwNI CAG_nnn'CTG
Cuts at: 448 1038 1930 3497 448
Size: 590 892 1567 1576
Fragments arranged by size:
1576 1567 892 590
ApaI G_GGCC'C
Cuts at: 193 193
Size: 4625
ApaLI G'TGCA_C
Cuts at: 271 3395 271
Size: 3124 1501
ApoI r'AATT_y
Cuts at: 842 1122 1629 3978 4234 842
Size: 280 507 2349 256 1233
Fragments arranged by size:
2349 1233 507 280 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 115 205 1210 1286 1645 115
Size: 90 1005 76 359 3095
Fragments arranged by size:
3095 1005 359 90 76
BamHI G'GATC_C
Cuts at: 111 1650 111
Size: 1539 3086
BanI G'GyrC_C
Cuts at: 216 237 246 1273 1641 2055 2303 2338 216
Size: 21 9 1027 368 414 248 35 2503
Fragments arranged by size:
2503 1027 414 368 248 35 21 9
BanII G_rGCy'C
Cuts at: 193 410 459 483 976 1639 2669 193
Size: 217 49 24 493 663 1030 2149
Fragments arranged by size:
2149 1030 663 493 217 49 24
BbeI G_GCGC'C
Cuts at: 241 2059 2307 241
Size: 1818 248 2559
Fragments arranged by size:
2559 1818 248
BbsI GAAGACnn'nnnn_
Cuts at: 587 1196 587
Size: 609 4016
BciVI GTATCCnnnnn_n'
Cuts at: 1371 2523 3284 4625 1371
Size: 1152 761 1341 1371
Fragments arranged by size:
1371 1341 1152 761
BclI T'GATC_A
Cuts at: 2145 2145
Size: 4625
BglI GCCn_nnn'nGGC
Cuts at: 366 366
Size: 4625
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 578 1737 578
Size: 1159 3466
Bme1580I G_kGCm'C
Cuts at: 193 275 350 362 2250 2343 3399 193
Size: 82 75 12 1888 93 1056 1419
Fragments arranged by size:
1888 1419 1056 93 82 75 12
BmgBI CAC'GTC
Cuts at: 276 276
Size: 4625
BmrI ACTGGGnnnn_n'
Cuts at: 1837 1918 2253 4397 1837
Size: 81 335 2144 2065
Fragments arranged by size:
2144 2065 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 476 563 923 1192 322
Size: 154 87 360 269 3755
Fragments arranged by size:
3755 360 269 154 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1801 2680 3172 3470 1801
Size: 879 492 298 2956
Fragments arranged by size:
2956 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 286 4329 286
Size: 4043 582
BsaAI yAC'GTr
Cuts at: 1978 1978
Size: 4625
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 238 986 2056 2304 3006 4502 238
Size: 748 1070 248 702 1496 361
Fragments arranged by size:
1496 1070 748 702 361 248
BsaWI w'CCGG_w
Cuts at: 199 371 1814 2335 3013 3287 3434 3845 199
Size: 172 1443 521 678 274 147 411 979
Fragments arranged by size:
1443 979 678 521 411 274 172 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1525 1525
Size: 4625
BseYI C'CCAG_C
Cuts at: 558 1111 2050 2808 3385 3881 558
Size: 553 939 758 577 496 1302
Fragments arranged by size:
1302 939 758 577 553 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 787
Size: 4625
BsiEI CG_ry'CG
Cuts at: 104 2167 2213 3421 104
Size: 2063 46 1208 1308
Fragments arranged by size:
2063 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 275 483 939 1639 2417 2607 3399 275
Size: 208 456 700 778 190 792 1501
Fragments arranged by size:
1501 792 778 700 456 208 190
Bsp1286I G_dGCh'C
Cuts at: 193 275 350 362 410 459 483 939 976
Size: 82 75 12 48 49 24 456 37
Cuts at: 976 1639 2250 2343 2417 2607 2669 3399 193
Size: 663 611 93 74 190 62 730 1419
Fragments arranged by size:
1419 730 663 611 456 190 93 82
75 74 62 49 48 37 24 12
BspEI T'CCGG_A
Cuts at: 199 371 3845 199
Size: 172 3474 979
Fragments arranged by size:
3474 979 172
BspHI T'CATG_A
Cuts at: 323 3801 323
Size: 3478 1147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 622 1675 1889 2191 2572 622
Size: 1053 214 302 381 2675
Fragments arranged by size:
2675 1053 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2917 2971 4455 4620 50
Size: 2867 54 1484 165 55
Fragments arranged by size:
2867 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1100 2537 1100
Size: 1437 3188
BsrFI r'CCGG_y
Cuts at: 138 213 2623 2804 3013 138
Size: 75 2410 181 209 1750
Fragments arranged by size:
2410 1750 209 181 75
BssSI C'ACGA_G
Cuts at: 2896 3254 2896
Size: 358 4267
BstAPI GCAn_nnn'nTGC
Cuts at: 448 1023 1038 1080 1095 2002 448
Size: 575 15 42 15 907 3071
Fragments arranged by size:
3071 907 575 42 15 15
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 635 635
Size: 4625
BstYI r'GATC_y
Cuts at: 1 111 1254 1650 2117 2140 2475 2721 3733
Size: 110 1143 396 467 23 335 246 1012
Cuts at: 3733 3819 3831 4177 1
Size: 86 12 346 449
Fragments arranged by size:
1143 1012 467 449 396 346 335 246
110 86 23 12
BtgI C'CryG_G
Cuts at: 107 221 279 107
Size: 114 58 4453
Fragments arranged by size:
4453 114 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 446 1868 446
Size: 1422 3203
ClaI AT'CG_AT
Cuts at: 686 686
Size: 4625
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3189 2331
Size: 858 3767
EaeI y'GGCC_r
Cuts at: 338 2210 2384 2775 2802 4147 338
Size: 1872 174 391 27 1345 816
Fragments arranged by size:
1872 1345 816 391 174 27
EagI C'GGCC_G
Cuts at: 2210 2210
Size: 4625
EarI CTCTTCn'nnn_
Cuts at: 316 806 2648 2858 316
Size: 490 1842 210 2083
Fragments arranged by size:
2083 1842 490 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2833 3143 3289 4435 2833
Size: 310 146 1146 3023
Fragments arranged by size:
3023 1146 310 146
EcoICRI GAG'CTC
Cuts at: 481 1637 481
Size: 1156 3469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 476 563 776 923 1192 2450 2882 3629
Size: 154 87 213 147 269 1258 432 747
Cuts at: 3629 3897 322
Size: 268 1050
Fragments arranged by size:
1258 1050 747 432 269 268 213 154
147 87
EcoO109I rG'GnC_Cy
Cuts at: 189 708 796 1764 189
Size: 519 88 968 3050
Fragments arranged by size:
3050 968 519 88
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1099 2644 1099
Size: 1545 3080
FspI TGC'GCA
Cuts at: 2406 2406
Size: 4625
HaeII r_GCGC'y
Cuts at: 241 310 2059 2307 3329 4308 241
Size: 69 1749 248 1022 979 558
Fragments arranged by size:
1749 1022 979 558 248 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 397 429 912 944 1179 1211 1509 1541 1552
Size: 32 483 32 235 32 298 32 11
Cuts at: 1552 1584 2467 2497 2499 2529 397
Size: 32 883 30 2 30 2493
Fragments arranged by size:
2493 883 483 298 235 32 32 32
32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 1664 1664
Size: 4625
HindIII A'AGCT_T
Cuts at: 1535 1602 1535
Size: 67 4558
KasI G'GCGC_C
Cuts at: 237 2055 2303 237
Size: 1818 248 2559
Fragments arranged by size:
2559 1818 248
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1031 3296 3480 1031
Size: 2265 184 2176
Fragments arranged by size:
2265 2176 184
MscI TGG'CCA
Cuts at: 2386 2386
Size: 4625
MslI CAynn'nnrTG
Cuts at: 2614 2741 2614
Size: 127 4498
MspA1I CmG'CkG
Cuts at: 223 281 1736 2050 2410 3423 3668 223
Size: 58 1455 314 360 1013 245 1180
Fragments arranged by size:
1455 1180 1013 360 314 245 58
NaeI GCC'GGC
Cuts at: 2806 2806
Size: 4625
NarI GG'CG_CC
Cuts at: 238 2056 2304 238
Size: 1818 248 2559
Fragments arranged by size:
2559 1818 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4625
NgoMIV G'CCGG_C
Cuts at: 2804 2804
Size: 4625
NspI r_CATG'y
Cuts at: 475 1678 2178 3085 475
Size: 1203 500 907 2015
Fragments arranged by size:
2015 1203 907 500
PasI CC'CwG_GG
Cuts at: 147 147
Size: 4625
PciI A'CATG_T
Cuts at: 3081 3081
Size: 4625
PflMI CCAn_nnn'nTGG
Cuts at: 878 878
Size: 4625
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 708 796 708
Size: 88 4537
PspOMI G'GGCC_C
Cuts at: 189 189
Size: 4625
PstI C_TGCA'G
Cuts at: 453 506 1034 1043 1532 1672 2357 453
Size: 53 528 9 489 140 685 2721
Fragments arranged by size:
2721 685 528 489 140 53 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 2050 2410 2050
Size: 360 4265
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 483 1639 483
Size: 1156 3469
SacII CC_GC'GG
Cuts at: 224 282 224
Size: 58 4567
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 316 2648 2858 316
Size: 2332 210 2083
Fragments arranged by size:
2332 2083 210
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 435 4142 435
Size: 3707 918
SfcI C'TryA_G
Cuts at: 33 449 502 1030 1039 1326 1528 1668 2353
Size: 416 53 528 9 287 202 140 685
Cuts at: 2353 3346 3537 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 528 416 287 202 191
140 53 9
SfoI GGC'GCC
Cuts at: 239 2057 2305 239
Size: 1818 248 2559
Fragments arranged by size:
2559 1818 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 117 207 1212 1647 117
Size: 90 1005 435 3095
Fragments arranged by size:
3095 1005 435 90
SmlI C'TyrA_G
Cuts at: 1286 1780 2695 3187 3449 1286
Size: 494 915 492 262 2462
Fragments arranged by size:
2462 915 494 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 1678 2178 1678
Size: 500 4125
StyI C'CwwG_G
Cuts at: 107 153 889 1470 1759 2113 107
Size: 46 736 581 289 354 2619
Fragments arranged by size:
2619 736 581 354 289 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 310 585 982 2208 2341 2800 3009 310
Size: 275 397 1226 133 459 209 1926
Fragments arranged by size:
1926 1226 459 397 275 209 133
TatI w'GTAC_w
Cuts at: 433 1297 4140 433
Size: 864 2843 918
Fragments arranged by size:
2843 918 864
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4142 4174 4142
Size: 32 4593
Tth111I GACn'n_nGTC
Cuts at: 2422 2422
Size: 4625
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2120 2120
Size: 4625
XhoI C'TCGA_G
Cuts at: 1286 1286
Size: 4625
XmaI C'CCGG_G
Cuts at: 115 205 1210 1645 115
Size: 90 1005 435 3095
Fragments arranged by size:
3095 1005 435 90
XmnI GAAnn'nnTTC
Cuts at: 1082 1426 1448 1604 1082
Size: 344 22 156 4103
Fragments arranged by size:
4103 344 156 22
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI