(Circular) (Six-base) MAPSORT of: pF1KB9409.seq Check: 2427 from: 1 to: 3980 pF1KB9409 3980 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AccI GT'mk_AC Cuts at: 1018 1018 Size: 3980 Acc65I G'GTAC_C Cuts at: 996 996 Size: 3980 AclI AA'CG_TT Cuts at: 3820 3820 Size: 3980 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1805 2237 2590 3252 1805 Size: 432 353 662 2533 Fragments arranged by size: 2533 662 432 353 AfeI AGC'GCT Cuts at: 214 214 Size: 3980 AflIII A'CryG_T Cuts at: 1332 3132 3413 1332 Size: 1800 281 1899 Fragments arranged by size: 1899 1800 281 AleI CACnn'nnGTG Cuts at: 823 823 Size: 3980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 166 166 Size: 3980 AlwNI CAG_nnn'CTG Cuts at: 195 513 1285 2723 195 Size: 318 772 1438 1452 Fragments arranged by size: 1452 1438 772 318 ApaLI G'TGCA_C Cuts at: 2818 2818 Size: 3980 ApoI r'AATT_y Cuts at: 984 3333 3589 984 Size: 2349 256 1375 Fragments arranged by size: 2349 1375 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3980 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3980 AvaI C'yCGr_G Cuts at: 1000 1000 Size: 3980 BamHI G'GATC_C Cuts at: 910 1005 910 Size: 95 3885 BanI G'GyrC_C Cuts at: 996 1410 1658 1693 996 Size: 414 248 35 3283 Fragments arranged by size: 3283 414 248 35 BanII G_rGCy'C Cuts at: 749 772 994 2024 749 Size: 23 222 1030 2705 Fragments arranged by size: 2705 1030 222 23 BbeI G_GCGC'C Cuts at: 1414 1662 1414 Size: 248 3732 BbsI GAAGACnn'nnnn_ Cuts at: 327 327 Size: 3980 BciVI GTATCCnnnnn_n' Cuts at: 1878 2934 3980 1878 Size: 1056 1046 1878 Fragments arranged by size: 1878 1056 1046 BclI T'GATC_A Cuts at: 1500 1500 Size: 3980 BglII A'GATC_T Cuts at: 1495 3532 1495 Size: 2037 1943 BlpI GC'TnA_GC Cuts at: 1092 1092 Size: 3980 Bme1580I G_kGCm'C Cuts at: 1605 1698 2822 1605 Size: 93 1124 2763 Fragments arranged by size: 2763 1124 93 BmgBI CAC'GTC Cuts at: 825 957 825 Size: 132 3848 BmrI ACTGGGnnnn_n' Cuts at: 1192 1273 1608 3752 1192 Size: 81 335 2144 1420 Fragments arranged by size: 2144 1420 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2347 2347 Size: 3980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 319 538 733 319 Size: 219 195 3566 Fragments arranged by size: 3566 219 195 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1156 2035 2749 3047 1156 Size: 879 714 298 2089 Fragments arranged by size: 2089 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 442 3684 442 Size: 3242 738 BsaAI yAC'GTr Cuts at: 1333 1333 Size: 3980 BsaBI GATnn'nnATC Cuts at: 6 1494 3531 6 Size: 1488 2037 455 Fragments arranged by size: 2037 1488 455 BsaHI Gr'CG_yC Cuts at: 1411 1659 2361 3857 1411 Size: 248 702 1496 1534 Fragments arranged by size: 1534 1496 702 248 BsaWI w'CCGG_w Cuts at: 1169 1690 2368 2779 2926 3200 1169 Size: 521 678 411 147 274 1949 Fragments arranged by size: 1949 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 103 133 460 490 613 643 103 Size: 30 327 30 123 30 3440 Fragments arranged by size: 3440 327 123 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 223 563 859 223 Size: 340 296 3344 Fragments arranged by size: 3344 340 296 BseYI C'CCAG_C Cuts at: 373 537 611 1405 2163 2828 3236 373 Size: 164 74 794 758 665 408 1117 Fragments arranged by size: 1117 794 758 665 408 164 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 268 268 Size: 3980 BsiEI CG_ry'CG Cuts at: 104 180 1522 1568 2798 104 Size: 76 1342 46 1230 1286 Fragments arranged by size: 1342 1286 1230 76 46 BsiHKAI G_wGCw'C Cuts at: 232 593 772 869 994 1772 1962 2822 232 Size: 361 179 97 125 778 190 860 1390 Fragments arranged by size: 1390 860 778 361 190 179 125 97 BsmBI CGTCTCn'nnnn_ Cuts at: 167 167 Size: 3980 Bsp1286I G_dGCh'C Cuts at: 232 593 749 772 869 994 1605 1698 1772 Size: 361 156 23 97 125 611 93 74 Cuts at: 1772 1962 2024 2822 232 Size: 190 62 798 1390 Fragments arranged by size: 1390 798 611 361 190 156 125 97 93 74 62 23 BspHI T'CATG_A Cuts at: 576 2412 576 Size: 1836 2144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 173 203 1030 1244 1546 1927 173 Size: 30 827 214 302 381 2226 Fragments arranged by size: 2226 827 381 302 214 30 BsrBI CCG'CTC Cuts at: 50 2272 2326 3810 3975 50 Size: 2222 54 1484 165 55 Fragments arranged by size: 2222 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 659 1892 659 Size: 1233 2747 BsrFI r'CCGG_y Cuts at: 1978 2159 1978 Size: 181 3799 BssSI C'ACGA_G Cuts at: 124 2251 2959 124 Size: 2127 708 1145 Fragments arranged by size: 2127 1145 708 BstAPI GCAn_nnn'nTGC Cuts at: 195 597 1357 195 Size: 402 760 2818 Fragments arranged by size: 2818 760 402 BstBI TT'CG_AA Cuts at: 2341 2341 Size: 3980 BstYI r'GATC_y Cuts at: 1 910 1005 1472 1495 1830 2076 2382 2394 Size: 909 95 467 23 335 246 306 12 Cuts at: 2394 2480 3532 1 Size: 86 1052 449 Fragments arranged by size: 1052 909 467 449 335 306 246 95 86 23 12 Bsu36I CC'TnA_GG Cuts at: 506 506 Size: 3980 BtgI C'CryG_G Cuts at: 107 464 107 Size: 357 3623 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1930 2111 2274 1930 Size: 181 163 3636 Fragments arranged by size: 3636 181 163 BtsI GCAGTG_nn' Cuts at: 232 1223 232 Size: 991 2989 DraI TTT'AAA Cuts at: 979 979 Size: 3980 DraIII CAC_nnn'GTG Cuts at: 129 129 Size: 3980 DrdI GACnn_nn'nnGTC Cuts at: 181 390 1686 3030 181 Size: 209 1296 1344 1131 Fragments arranged by size: 1344 1296 1131 209 EaeI y'GGCC_r Cuts at: 284 344 1565 1739 2130 2157 3502 284 Size: 60 1221 174 391 27 1345 762 Fragments arranged by size: 1345 1221 762 391 174 60 27 EagI C'GGCC_G Cuts at: 1565 1565 Size: 3980 EarI CTCTTCn'nnn_ Cuts at: 323 2003 2213 323 Size: 1680 210 2090 Fragments arranged by size: 2090 1680 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2188 2930 3076 3790 2188 Size: 742 146 714 2378 Fragments arranged by size: 2378 742 714 146 EcoICRI GAG'CTC Cuts at: 770 992 770 Size: 222 3758 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 319 538 733 1805 2237 2590 3252 319 Size: 219 195 1072 432 353 662 1047 Fragments arranged by size: 1072 1047 662 432 353 219 195 EcoO109I rG'GnC_Cy Cuts at: 378 1119 378 Size: 741 3239 EcoRI G'AATT_C Cuts at: 984 984 Size: 3980 EcoRV GAT'ATC Cuts at: 966 966 Size: 3980 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1999 1999 Size: 3980 FspI TGC'GCA Cuts at: 274 601 1761 274 Size: 327 1160 2493 Fragments arranged by size: 2493 1160 327 HaeII r_GCGC'y Cuts at: 216 1414 1662 2892 3663 216 Size: 1198 248 1230 771 533 Fragments arranged by size: 1230 1198 771 533 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 613 645 722 754 1822 1852 1854 1884 613 Size: 32 77 32 1068 30 2 30 2709 Fragments arranged by size: 2709 1068 77 32 32 30 30 2 HincII GTy'rAC Cuts at: 1019 1019 Size: 3980 HindIII A'AGCT_T Cuts at: 839 839 Size: 3980 KasI G'GCGC_C Cuts at: 1410 1658 1410 Size: 248 3732 KpnI G_GTAC'C Cuts at: 1000 1000 Size: 3980 MluI A'CGCG_T Cuts at: 3413 3413 Size: 3980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 285 899 2739 2923 285 Size: 614 1840 184 1342 Fragments arranged by size: 1840 1342 614 184 MscI TGG'CCA Cuts at: 286 346 1741 286 Size: 60 1395 2525 Fragments arranged by size: 2525 1395 60 MslI CAynn'nnrTG Cuts at: 292 823 1969 2096 292 Size: 531 1146 127 2176 Fragments arranged by size: 2176 1146 531 127 MspA1I CmG'CkG Cuts at: 459 466 565 618 1091 1405 1765 2549 2794 Size: 7 99 53 473 314 360 784 245 Cuts at: 2794 459 Size: 1645 Fragments arranged by size: 1645 784 473 360 314 245 99 53 7 NaeI GCC'GGC Cuts at: 2161 2161 Size: 3980 NarI GG'CG_CC Cuts at: 1411 1659 1411 Size: 248 3732 NcoI C'CATG_G Cuts at: 107 107 Size: 3980 NgoMIV G'CCGG_C Cuts at: 2159 2159 Size: 3980 NspI r_CATG'y Cuts at: 273 426 649 1033 1533 3136 273 Size: 153 223 384 500 1603 1117 Fragments arranged by size: 1603 1117 500 384 223 153 PciI A'CATG_T Cuts at: 3132 3132 Size: 3980 PflMI CCAn_nnn'nTGG Cuts at: 129 129 Size: 3980 PmeI GTTT'AAAC Cuts at: 979 979 Size: 3980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2426 2458 2426 Size: 32 3948 PpuMI rG'GwC_Cy Cuts at: 378 378 Size: 3980 PstI C_TGCA'G Cuts at: 191 884 1027 1712 191 Size: 693 143 685 2459 Fragments arranged by size: 2459 693 685 143 PvuI CG_AT'CG Cuts at: 104 104 Size: 3980 PvuII CAG'CTG Cuts at: 565 1405 1765 565 Size: 840 360 2780 Fragments arranged by size: 2780 840 360 RsrII CG'GwC_CG Cuts at: 138 2175 138 Size: 2037 1943 SacI G_AGCT'C Cuts at: 772 994 772 Size: 222 3758 SacII CC_GC'GG Cuts at: 467 467 Size: 3980 SalI G'TCGA_C Cuts at: 1017 1017 Size: 3980 SanDI GG'GwC_CC Cuts at: 378 378 Size: 3980 SapI GCTCTTCn'nnn_ Cuts at: 2003 2213 2003 Size: 210 3770 SbfI CC_TGCA'GG Cuts at: 1027 1027 Size: 3980 ScaI AGT'ACT Cuts at: 3497 3497 Size: 3980 SexAI A'CCwGG_T Cuts at: 971 971 Size: 3980 SfcI C'TryA_G Cuts at: 33 187 880 950 1023 1708 2676 2867 33 Size: 154 693 70 73 685 968 191 1146 Fragments arranged by size: 1146 968 693 685 191 154 73 70 SfoI GGC'GCC Cuts at: 1412 1660 1412 Size: 248 3732 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3980 SmaI CCC'GGG Cuts at: 1002 1002 Size: 3980 SmlI C'TyrA_G Cuts at: 1135 2050 2764 3026 1135 Size: 915 714 262 2089 Fragments arranged by size: 2089 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3980 SphI G_CATG'C Cuts at: 649 1033 1533 649 Size: 384 500 3096 Fragments arranged by size: 3096 500 384 StyI C'CwwG_G Cuts at: 107 1114 1468 107 Size: 1007 354 2619 Fragments arranged by size: 2619 1007 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 400 1563 1696 2155 2364 400 Size: 1163 133 459 209 2016 Fragments arranged by size: 2016 1163 459 209 133 TatI w'GTAC_w Cuts at: 3495 3495 Size: 3980 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 103 135 304 336 460 492 3497 3529 103 Size: 32 169 32 124 32 3005 32 554 Fragments arranged by size: 3005 554 169 124 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 136 181 1777 136 Size: 45 1596 2339 Fragments arranged by size: 2339 1596 45 XbaI T'CTAG_A Cuts at: 1011 1011 Size: 3980 XcmI CCAnnnn_n'nnnnTGG Cuts at: 294 354 822 1475 294 Size: 60 468 653 2799 Fragments arranged by size: 2799 653 468 60 XmaI C'CCGG_G Cuts at: 1000 1000 Size: 3980 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AgeI AhdI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI BsmI BspEI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI