Restriction map for pF1KB9409
 (Circular) (Six-base) MAPSORT of: pF1KB9409.seq  Check: 2427  from: 1  to: 3980



pF1KB9409  3980 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AccI GT'mk_AC

Cuts at:   1018    1018 
   Size:       3980
 
Acc65I G'GTAC_C

Cuts at:    996     996 
   Size:       3980
 
AclI AA'CG_TT

Cuts at:   3820    3820 
   Size:       3980
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1805    2237    2590    3252    1805 
   Size:        432     353     662    2533

  Fragments arranged by size: 

                2533     662     432     353
 
AfeI AGC'GCT

Cuts at:    214     214 
   Size:       3980
 
AflIII A'CryG_T

Cuts at:   1332    3132    3413    1332 
   Size:       1800     281    1899

  Fragments arranged by size: 

                1899    1800     281
 
AleI CACnn'nnGTG

Cuts at:    823     823 
   Size:       3980
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    166     166 
   Size:       3980
 
AlwNI CAG_nnn'CTG

Cuts at:    195     513    1285    2723     195 
   Size:        318     772    1438    1452

  Fragments arranged by size: 

                1452    1438     772     318
 
ApaLI G'TGCA_C

Cuts at:   2818    2818 
   Size:       3980
 
ApoI r'AATT_y

Cuts at:    984    3333    3589     984 
   Size:       2349     256    1375

  Fragments arranged by size: 

                2349    1375     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3980
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3980
 
AvaI C'yCGr_G

Cuts at:   1000    1000 
   Size:       3980
 
BamHI G'GATC_C

Cuts at:    910    1005     910 
   Size:         95    3885
 
BanI G'GyrC_C

Cuts at:    996    1410    1658    1693     996 
   Size:        414     248      35    3283

  Fragments arranged by size: 

                3283     414     248      35
 
BanII G_rGCy'C

Cuts at:    749     772     994    2024     749 
   Size:         23     222    1030    2705

  Fragments arranged by size: 

                2705    1030     222      23
 
BbeI G_GCGC'C

Cuts at:   1414    1662    1414 
   Size:        248    3732
 
BbsI GAAGACnn'nnnn_

Cuts at:    327     327 
   Size:       3980
 
BciVI GTATCCnnnnn_n'

Cuts at:   1878    2934    3980    1878 
   Size:       1056    1046    1878

  Fragments arranged by size: 

                1878    1056    1046
 
BclI T'GATC_A

Cuts at:   1500    1500 
   Size:       3980
 
BglII A'GATC_T

Cuts at:   1495    3532    1495 
   Size:       2037    1943
 
BlpI GC'TnA_GC

Cuts at:   1092    1092 
   Size:       3980
 
Bme1580I G_kGCm'C

Cuts at:   1605    1698    2822    1605 
   Size:         93    1124    2763

  Fragments arranged by size: 

                2763    1124      93
 
BmgBI CAC'GTC

Cuts at:    825     957     825 
   Size:        132    3848
 
BmrI ACTGGGnnnn_n'

Cuts at:   1192    1273    1608    3752    1192 
   Size:         81     335    2144    1420

  Fragments arranged by size: 

                2144    1420     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2347    2347 
   Size:       3980
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    319     538     733     319 
   Size:        219     195    3566

  Fragments arranged by size: 

                3566     219     195
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1156    2035    2749    3047    1156 
   Size:        879     714     298    2089

  Fragments arranged by size: 

                2089     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    442    3684     442 
   Size:       3242     738
 
BsaAI yAC'GTr

Cuts at:   1333    1333 
   Size:       3980
 
BsaBI GATnn'nnATC

Cuts at:      6    1494    3531       6 
   Size:       1488    2037     455

  Fragments arranged by size: 

                2037    1488     455
 
BsaHI Gr'CG_yC

Cuts at:   1411    1659    2361    3857    1411 
   Size:        248     702    1496    1534

  Fragments arranged by size: 

                1534    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1169    1690    2368    2779    2926    3200    1169 
   Size:        521     678     411     147     274    1949

  Fragments arranged by size: 

                1949     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    103     133     460     490     613     643     103 
   Size:         30     327      30     123      30    3440

  Fragments arranged by size: 

                3440     327     123      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    223     563     859     223 
   Size:        340     296    3344

  Fragments arranged by size: 

                3344     340     296
 
BseYI C'CCAG_C

Cuts at:    373     537     611    1405    2163    2828    3236     373 
   Size:        164      74     794     758     665     408    1117

  Fragments arranged by size: 

                1117     794     758     665     408     164      74
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    268     268 
   Size:       3980
 
BsiEI CG_ry'CG

Cuts at:    104     180    1522    1568    2798     104 
   Size:         76    1342      46    1230    1286

  Fragments arranged by size: 

                1342    1286    1230      76      46
 
BsiHKAI G_wGCw'C

Cuts at:    232     593     772     869     994    1772    1962    2822     232 
   Size:        361     179      97     125     778     190     860    1390

  Fragments arranged by size: 

                1390     860     778     361     190     179     125      97
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:    167     167 
   Size:       3980
 
Bsp1286I G_dGCh'C

Cuts at:    232     593     749     772     869     994    1605    1698    1772 
   Size:        361     156      23      97     125     611      93      74

Cuts at:   1772    1962    2024    2822     232 
   Size:        190      62     798    1390

  Fragments arranged by size: 

                1390     798     611     361     190     156     125      97
                  93      74      62      23
 
BspHI T'CATG_A

Cuts at:    576    2412     576 
   Size:       1836    2144
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    173     203    1030    1244    1546    1927     173 
   Size:         30     827     214     302     381    2226

  Fragments arranged by size: 

                2226     827     381     302     214      30
 
BsrBI CCG'CTC

Cuts at:     50    2272    2326    3810    3975      50 
   Size:       2222      54    1484     165      55

  Fragments arranged by size: 

                2222    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    659    1892     659 
   Size:       1233    2747
 
BsrFI r'CCGG_y

Cuts at:   1978    2159    1978 
   Size:        181    3799
 
BssSI C'ACGA_G

Cuts at:    124    2251    2959     124 
   Size:       2127     708    1145

  Fragments arranged by size: 

                2127    1145     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:    195     597    1357     195 
   Size:        402     760    2818

  Fragments arranged by size: 

                2818     760     402
 
BstBI TT'CG_AA

Cuts at:   2341    2341 
   Size:       3980
 
BstYI r'GATC_y

Cuts at:      1     910    1005    1472    1495    1830    2076    2382    2394 
   Size:        909      95     467      23     335     246     306      12

Cuts at:   2394    2480    3532       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1052     909     467     449     335     306     246      95
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    506     506 
   Size:       3980
 
BtgI C'CryG_G

Cuts at:    107     464     107 
   Size:        357    3623
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1930    2111    2274    1930 
   Size:        181     163    3636

  Fragments arranged by size: 

                3636     181     163
 
BtsI GCAGTG_nn'

Cuts at:    232    1223     232 
   Size:        991    2989
 
DraI TTT'AAA

Cuts at:    979     979 
   Size:       3980
 
DraIII CAC_nnn'GTG

Cuts at:    129     129 
   Size:       3980
 
DrdI GACnn_nn'nnGTC

Cuts at:    181     390    1686    3030     181 
   Size:        209    1296    1344    1131

  Fragments arranged by size: 

                1344    1296    1131     209
 
EaeI y'GGCC_r

Cuts at:    284     344    1565    1739    2130    2157    3502     284 
   Size:         60    1221     174     391      27    1345     762

  Fragments arranged by size: 

                1345    1221     762     391     174      60      27
 
EagI C'GGCC_G

Cuts at:   1565    1565 
   Size:       3980
 
EarI CTCTTCn'nnn_

Cuts at:    323    2003    2213     323 
   Size:       1680     210    2090

  Fragments arranged by size: 

                2090    1680     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2188    2930    3076    3790    2188 
   Size:        742     146     714    2378

  Fragments arranged by size: 

                2378     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    770     992     770 
   Size:        222    3758
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    319     538     733    1805    2237    2590    3252     319 
   Size:        219     195    1072     432     353     662    1047

  Fragments arranged by size: 

                1072    1047     662     432     353     219     195
 
EcoO109I rG'GnC_Cy

Cuts at:    378    1119     378 
   Size:        741    3239
 
EcoRI G'AATT_C

Cuts at:    984     984 
   Size:       3980
 
EcoRV GAT'ATC

Cuts at:    966     966 
   Size:       3980
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1999    1999 
   Size:       3980
 
FspI TGC'GCA

Cuts at:    274     601    1761     274 
   Size:        327    1160    2493

  Fragments arranged by size: 

                2493    1160     327
 
HaeII r_GCGC'y

Cuts at:    216    1414    1662    2892    3663     216 
   Size:       1198     248    1230     771     533

  Fragments arranged by size: 

                1230    1198     771     533     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    613     645     722     754    1822    1852    1854    1884     613 
   Size:         32      77      32    1068      30       2      30    2709

  Fragments arranged by size: 

                2709    1068      77      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1019    1019 
   Size:       3980
 
HindIII A'AGCT_T

Cuts at:    839     839 
   Size:       3980
 
KasI G'GCGC_C

Cuts at:   1410    1658    1410 
   Size:        248    3732
 
KpnI G_GTAC'C

Cuts at:   1000    1000 
   Size:       3980
 
MluI A'CGCG_T

Cuts at:   3413    3413 
   Size:       3980
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    285     899    2739    2923     285 
   Size:        614    1840     184    1342

  Fragments arranged by size: 

                1840    1342     614     184
 
MscI TGG'CCA

Cuts at:    286     346    1741     286 
   Size:         60    1395    2525

  Fragments arranged by size: 

                2525    1395      60
 
MslI CAynn'nnrTG

Cuts at:    292     823    1969    2096     292 
   Size:        531    1146     127    2176

  Fragments arranged by size: 

                2176    1146     531     127
 
MspA1I CmG'CkG

Cuts at:    459     466     565     618    1091    1405    1765    2549    2794 
   Size:          7      99      53     473     314     360     784     245

Cuts at:   2794     459 
   Size:       1645

  Fragments arranged by size: 

                1645     784     473     360     314     245      99      53
                   7
 
NaeI GCC'GGC

Cuts at:   2161    2161 
   Size:       3980
 
NarI GG'CG_CC

Cuts at:   1411    1659    1411 
   Size:        248    3732
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3980
 
NgoMIV G'CCGG_C

Cuts at:   2159    2159 
   Size:       3980
 
NspI r_CATG'y

Cuts at:    273     426     649    1033    1533    3136     273 
   Size:        153     223     384     500    1603    1117

  Fragments arranged by size: 

                1603    1117     500     384     223     153
 
PciI A'CATG_T

Cuts at:   3132    3132 
   Size:       3980
 
PflMI CCAn_nnn'nTGG

Cuts at:    129     129 
   Size:       3980
 
PmeI GTTT'AAAC

Cuts at:    979     979 
   Size:       3980
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2426    2458    2426 
   Size:         32    3948
 
PpuMI rG'GwC_Cy

Cuts at:    378     378 
   Size:       3980
 
PstI C_TGCA'G

Cuts at:    191     884    1027    1712     191 
   Size:        693     143     685    2459

  Fragments arranged by size: 

                2459     693     685     143
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3980
 
PvuII CAG'CTG

Cuts at:    565    1405    1765     565 
   Size:        840     360    2780

  Fragments arranged by size: 

                2780     840     360
 
RsrII CG'GwC_CG

Cuts at:    138    2175     138 
   Size:       2037    1943
 
SacI G_AGCT'C

Cuts at:    772     994     772 
   Size:        222    3758
 
SacII CC_GC'GG

Cuts at:    467     467 
   Size:       3980
 
SalI G'TCGA_C

Cuts at:   1017    1017 
   Size:       3980
 
SanDI GG'GwC_CC

Cuts at:    378     378 
   Size:       3980
 
SapI GCTCTTCn'nnn_

Cuts at:   2003    2213    2003 
   Size:        210    3770
 
SbfI CC_TGCA'GG

Cuts at:   1027    1027 
   Size:       3980
 
ScaI AGT'ACT

Cuts at:   3497    3497 
   Size:       3980
 
SexAI A'CCwGG_T

Cuts at:    971     971 
   Size:       3980
 
SfcI C'TryA_G

Cuts at:     33     187     880     950    1023    1708    2676    2867      33 
   Size:        154     693      70      73     685     968     191    1146

  Fragments arranged by size: 

                1146     968     693     685     191     154      73      70
            
 
SfoI GGC'GCC

Cuts at:   1412    1660    1412 
   Size:        248    3732
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3980
 
SmaI CCC'GGG

Cuts at:   1002    1002 
   Size:       3980
 
SmlI C'TyrA_G

Cuts at:   1135    2050    2764    3026    1135 
   Size:        915     714     262    2089

  Fragments arranged by size: 

                2089     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3980
 
SphI G_CATG'C

Cuts at:    649    1033    1533     649 
   Size:        384     500    3096

  Fragments arranged by size: 

                3096     500     384
 
StyI C'CwwG_G

Cuts at:    107    1114    1468     107 
   Size:       1007     354    2619

  Fragments arranged by size: 

                2619    1007     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    400    1563    1696    2155    2364     400 
   Size:       1163     133     459     209    2016

  Fragments arranged by size: 

                2016    1163     459     209     133
 
TatI w'GTAC_w

Cuts at:   3495    3495 
   Size:       3980
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    103     135     304     336     460     492    3497    3529     103 
   Size:         32     169      32     124      32    3005      32     554

  Fragments arranged by size: 

                3005     554     169     124      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:    136     181    1777     136 
   Size:         45    1596    2339

  Fragments arranged by size: 

                2339    1596      45
 
XbaI T'CTAG_A

Cuts at:   1011    1011 
   Size:       3980
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    294     354     822    1475     294 
   Size:         60     468     653    2799

  Fragments arranged by size: 

                2799     653     468      60
 
XmaI C'CCGG_G

Cuts at:   1000    1000 
   Size:       3980
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AleI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI
     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AgeI     AhdI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI     BmtI   Bpu10I
    BsiWI     BsmI    BspEI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
     ClaI    CspCI    EcoNI     FseI    FspAI     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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