Restriction map for
pF1KB9414
(Circular) (Six-base) MAPSORT of: pF1KB9414.seq Check: 9900 from: 1 to: 5252
pF1KB9414 5252 bp
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AccI GT'mk_AC
Cuts at: 2290 2290
Size: 5252
Acc65I G'GTAC_C
Cuts at: 2268 2268
Size: 5252
AclI AA'CG_TT
Cuts at: 5092 5092
Size: 5252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 929 1399 1946 2005 2070 2172 3077 3509
Size: 82 470 547 59 65 102 905 432
Cuts at: 3509 4256 4524 847
Size: 747 268 1575
Fragments arranged by size:
1575 905 747 547 470 432 268 102
82 65 59
AfeI AGC'GCT
Cuts at: 2052 2052
Size: 5252
AflII C'TTAA_G
Cuts at: 664 664
Size: 5252
AflIII A'CryG_T
Cuts at: 417 2604 3708 4685 417
Size: 2187 1104 977 984
Fragments arranged by size:
2187 1104 984 977
AgeI A'CCGG_T
Cuts at: 3640 3640
Size: 5252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1493 1493
Size: 5252
AlwNI CAG_nnn'CTG
Cuts at: 1196 1459 2557 4124 1196
Size: 263 1098 1567 2324
Fragments arranged by size:
2324 1567 1098 263
ApaLI G'TGCA_C
Cuts at: 1070 1865 4022 1070
Size: 795 2157 2300
Fragments arranged by size:
2300 2157 795
ApoI r'AATT_y
Cuts at: 239 2256 4605 4861 239
Size: 2017 2349 256 630
Fragments arranged by size:
2349 2017 630 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5252
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5252
AvaI C'yCGr_G
Cuts at: 2272 2272
Size: 5252
BamHI G'GATC_C
Cuts at: 1721 2277 1721
Size: 556 4696
BanI G'GyrC_C
Cuts at: 2268 2682 2930 2965 2268
Size: 414 248 35 4555
Fragments arranged by size:
4555 414 248 35
BanII G_rGCy'C
Cuts at: 167 2266 3296 167
Size: 2099 1030 2123
Fragments arranged by size:
2123 2099 1030
BbeI G_GCGC'C
Cuts at: 2686 2934 2686
Size: 248 5004
BbsI GAAGACnn'nnnn_
Cuts at: 140 299 614 1952 140
Size: 159 315 1338 3440
Fragments arranged by size:
3440 1338 315 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 223 257 223
Size: 34 5218
BciVI GTATCCnnnnn_n'
Cuts at: 3150 3911 5252 3150
Size: 761 1341 3150
Fragments arranged by size:
3150 1341 761
BclI T'GATC_A
Cuts at: 2772 2772
Size: 5252
BglII A'GATC_T
Cuts at: 2767 4804 2767
Size: 2037 3215
BlpI GC'TnA_GC
Cuts at: 2364 2364
Size: 5252
Bme1580I G_kGCm'C
Cuts at: 151 1074 1794 1869 2877 2970 4026 151
Size: 923 720 75 1008 93 1056 1377
Fragments arranged by size:
1377 1056 1008 923 720 93 75
BmrI ACTGGGnnnn_n'
Cuts at: 1240 2464 2545 2880 5024 1240
Size: 1224 81 335 2144 1468
Fragments arranged by size:
2144 1468 1224 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1553 3619 1553
Size: 2066 3186
Bpu10I CC'TnA_GC
Cuts at: 1350 1786 1350
Size: 436 4816
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2428 3307 3799 4097 2428
Size: 879 492 298 3583
Fragments arranged by size:
3583 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4956 4956
Size: 5252
BsaAI yAC'GTr
Cuts at: 418 2605 418
Size: 2187 3065
BsaBI GATnn'nnATC
Cuts at: 6 1994 2766 4803 6
Size: 1988 772 2037 455
Fragments arranged by size:
2037 1988 772 455
BsaHI Gr'CG_yC
Cuts at: 2683 2931 3633 5129 2683
Size: 248 702 1496 2806
Fragments arranged by size:
2806 1496 702 248
BsaWI w'CCGG_w
Cuts at: 599 2441 2962 3640 3914 4061 4472 599
Size: 1842 521 678 274 147 411 1379
Fragments arranged by size:
1842 1379 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1413 2123 1413
Size: 710 4542
BseYI C'CCAG_C
Cuts at: 297 980 1171 1932 2047 2197 2677 3435 4012
Size: 683 191 761 115 150 480 758 577
Cuts at: 4012 4508 297
Size: 496 1041
Fragments arranged by size:
1041 761 758 683 577 496 480 191
150 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2089 2089
Size: 5252
BsiEI CG_ry'CG
Cuts at: 104 2794 2840 4048 104
Size: 2690 46 1208 1308
Fragments arranged by size:
2690 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1074 1869 2266 3044 3234 4026 1074
Size: 795 397 778 190 792 2300
Fragments arranged by size:
2300 795 792 778 397 190
Bsp1286I G_dGCh'C
Cuts at: 151 167 1074 1794 1869 2266 2877 2970 3044
Size: 16 907 720 75 397 611 93 74
Cuts at: 3044 3234 3296 4026 151
Size: 190 62 730 1377
Fragments arranged by size:
1377 907 730 720 611 397 190 93
75 74 62 16
BspEI T'CCGG_A
Cuts at: 4472 4472
Size: 5252
BspHI T'CATG_A
Cuts at: 747 4428 747
Size: 3681 1571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2302 2516 2818 3199 2302
Size: 214 302 381 4355
Fragments arranged by size:
4355 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1558 3544 3598 5082 5247 50
Size: 1508 1986 54 1484 165 55
Fragments arranged by size:
1986 1508 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3164 3164
Size: 5252
BsrFI r'CCGG_y
Cuts at: 3250 3431 3640 3250
Size: 181 209 4862
Fragments arranged by size:
4862 209 181
BssSI C'ACGA_G
Cuts at: 3523 3881 3523
Size: 358 4894
BstAPI GCAn_nnn'nTGC
Cuts at: 2629 2629
Size: 5252
BstBI TT'CG_AA
Cuts at: 3613 3613
Size: 5252
BstYI r'GATC_y
Cuts at: 1 1721 2096 2277 2744 2767 3102 3348 4360
Size: 1720 375 181 467 23 335 246 1012
Cuts at: 4360 4446 4458 4804 1
Size: 86 12 346 449
Fragments arranged by size:
1720 1012 467 449 375 346 335 246
181 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3202 3383 3546 3202
Size: 181 163 4908
Fragments arranged by size:
4908 181 163
BtsI GCAGTG_nn'
Cuts at: 724 910 1880 2495 724
Size: 186 970 615 3481
Fragments arranged by size:
3481 970 615 186
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1004 1039 1803 1838 1004
Size: 35 764 35 4418
Fragments arranged by size:
4418 764 35 35
DraI TTT'AAA
Cuts at: 485 1760 2251 485
Size: 1275 491 3486
Fragments arranged by size:
3486 1275 491
DrdI GACnn_nn'nnGTC
Cuts at: 2958 3816 2958
Size: 858 4394
EaeI y'GGCC_r
Cuts at: 767 2837 3011 3402 3429 4774 767
Size: 2070 174 391 27 1345 1245
Fragments arranged by size:
2070 1345 1245 391 174 27
EagI C'GGCC_G
Cuts at: 2837 2837
Size: 5252
EarI CTCTTCn'nnn_
Cuts at: 320 1231 1418 1431 1988 3275 3485 320
Size: 911 187 13 557 1287 210 2087
Fragments arranged by size:
2087 1287 911 557 210 187 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3460 3770 3916 5062 3460
Size: 310 146 1146 3650
Fragments arranged by size:
3650 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2264 2264
Size: 5252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 929 1399 1946 2005 2070 2172 3077 3509
Size: 82 470 547 59 65 102 905 432
Cuts at: 3509 4256 4524 847
Size: 747 268 1575
Fragments arranged by size:
1575 905 747 547 470 432 268 102
82 65 59
EcoNI CCTnn'n_nnAGG
Cuts at: 1085 1085
Size: 5252
EcoO109I rG'GnC_Cy
Cuts at: 2025 2391 2025
Size: 366 4886
EcoRI G'AATT_C
Cuts at: 239 2256 239
Size: 2017 3235
EcoRV GAT'ATC
Cuts at: 183 989 183
Size: 806 4446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3271 3271
Size: 5252
FspI TGC'GCA
Cuts at: 3033 3033
Size: 5252
HaeII r_GCGC'y
Cuts at: 465 2054 2081 2686 2934 3956 4935 465
Size: 1589 27 605 248 1022 979 782
Fragments arranged by size:
1589 1022 979 782 605 248 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1093 1125 3094 3124 3126 3156 1093
Size: 32 1969 30 2 30 3189
Fragments arranged by size:
3189 1969 32 30 30 2
HincII GTy'rAC
Cuts at: 636 2291 636
Size: 1655 3597
KasI G'GCGC_C
Cuts at: 2682 2930 2682
Size: 248 5004
KpnI G_GTAC'C
Cuts at: 2272 2272
Size: 5252
MluI A'CGCG_T
Cuts at: 4685 4685
Size: 5252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1874 1901 2098 3923 4107 1874
Size: 27 197 1825 184 3019
Fragments arranged by size:
3019 1825 197 184 27
MscI TGG'CCA
Cuts at: 3013 3013
Size: 5252
MslI CAynn'nnrTG
Cuts at: 2035 3241 3368 2035
Size: 1206 127 3919
Fragments arranged by size:
3919 1206 127
MspA1I CmG'CkG
Cuts at: 1922 2103 2363 2677 3037 4050 4295 1922
Size: 181 260 314 360 1013 245 2879
Fragments arranged by size:
2879 1013 360 314 260 245 181
NaeI GCC'GGC
Cuts at: 3433 3433
Size: 5252
NarI GG'CG_CC
Cuts at: 2683 2931 2683
Size: 248 5004
NdeI CA'TA_TG
Cuts at: 552 552
Size: 5252
NgoMIV G'CCGG_C
Cuts at: 3431 3431
Size: 5252
NspI r_CATG'y
Cuts at: 275 741 2305 2805 3712 275
Size: 466 1564 500 907 1815
Fragments arranged by size:
1815 1564 907 500 466
PciI A'CATG_T
Cuts at: 3708 3708
Size: 5252
PflMI CCAn_nnn'nTGG
Cuts at: 1018 1196 1459 1018
Size: 178 263 4811
Fragments arranged by size:
4811 263 178
PmeI GTTT'AAAC
Cuts at: 2251 2251
Size: 5252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 4391
Size: 32 5220
PsiI TTA'TAA
Cuts at: 308 1565 308
Size: 1257 3995
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 809 841 809
Size: 32 5220
PstI C_TGCA'G
Cuts at: 697 917 1482 2299 2984 697
Size: 220 565 817 685 2965
Fragments arranged by size:
2965 817 685 565 220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5252
PvuII CAG'CTG
Cuts at: 1922 2677 3037 1922
Size: 755 360 4137
Fragments arranged by size:
4137 755 360
RsrII CG'GwC_CG
Cuts at: 3447 3447
Size: 5252
SacI G_AGCT'C
Cuts at: 2266 2266
Size: 5252
SalI G'TCGA_C
Cuts at: 2289 2289
Size: 5252
SapI GCTCTTCn'nnn_
Cuts at: 320 3275 3485 320
Size: 2955 210 2087
Fragments arranged by size:
2955 2087 210
SbfI CC_TGCA'GG
Cuts at: 2299 2299
Size: 5252
ScaI AGT'ACT
Cuts at: 4769 4769
Size: 5252
SexAI A'CCwGG_T
Cuts at: 1017 1017
Size: 5252
SfcI C'TryA_G
Cuts at: 33 693 913 1202 1259 1478 1727 1748 2221
Size: 660 220 289 57 219 249 21 473
Cuts at: 2221 2295 2980 3973 4164 33
Size: 74 685 993 191 1121
Fragments arranged by size:
1121 993 685 660 473 289 249 220
219 191 74 57 21
SfoI GGC'GCC
Cuts at: 2684 2932 2684
Size: 248 5004
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5252
SmaI CCC'GGG
Cuts at: 2274 2274
Size: 5252
SmlI C'TyrA_G
Cuts at: 664 2407 3322 3814 4076 664
Size: 1743 915 492 262 1840
Fragments arranged by size:
1840 1743 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5252
SphI G_CATG'C
Cuts at: 2305 2805 2305
Size: 500 4752
SspI AAT'ATT
Cuts at: 499 499
Size: 5252
StyI C'CwwG_G
Cuts at: 167 1603 2386 2740 167
Size: 1436 783 354 2679
Fragments arranged by size:
2679 1436 783 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2835 2968 3427 3636 2835
Size: 133 459 209 4451
Fragments arranged by size:
4451 459 209 133
TatI w'GTAC_w
Cuts at: 4767 4767
Size: 5252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1935 1967 4769 4801 1935
Size: 32 2802 32 2386
Fragments arranged by size:
2802 2386 32 32
Tth111I GACn'n_nGTC
Cuts at: 3049 3049
Size: 5252
XbaI T'CTAG_A
Cuts at: 2283 2283
Size: 5252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2747 2747
Size: 5252
XmaI C'CCGG_G
Cuts at: 2272 2272
Size: 5252
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI DraIII FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PspOMI PspXI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI