Restriction map for
pF1KB9415
(Circular) (Six-base) MAPSORT of: pF1KB9415.seq Check: 5102 from: 1 to: 5183
pF1KB9415 5183 bp
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AccI GT'mk_AC
Cuts at: 426 2221 426
Size: 1795 3388
Acc65I G'GTAC_C
Cuts at: 2075 2199 2075
Size: 124 5059
AclI AA'CG_TT
Cuts at: 5023 5023
Size: 5183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 1060 1292 3008 3440 4187 4455 152
Size: 908 232 1716 432 747 268 880
Fragments arranged by size:
1716 908 880 747 432 268 232
AflII C'TTAA_G
Cuts at: 2041 2041
Size: 5183
AflIII A'CryG_T
Cuts at: 214 500 2535 3639 4616 214
Size: 286 2035 1104 977 781
Fragments arranged by size:
2035 1104 977 781 286
AgeI A'CCGG_T
Cuts at: 3571 3571
Size: 5183
AlwNI CAG_nnn'CTG
Cuts at: 1782 2488 4055 1782
Size: 706 1567 2910
Fragments arranged by size:
2910 1567 706
ApaLI G'TGCA_C
Cuts at: 1464 3953 1464
Size: 2489 2694
ApoI r'AATT_y
Cuts at: 116 256 438 776 964 1024 1328 1446 1712
Size: 140 182 338 188 60 304 118 266
Cuts at: 1712 2187 4536 4792 116
Size: 475 2349 256 507
Fragments arranged by size:
2349 507 475 338 304 266 256 188
182 140 118 60
AseI AT'TA_AT
Cuts at: 20 698 20
Size: 678 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5183
AvaI C'yCGr_G
Cuts at: 595 2203 595
Size: 1608 3575
BamHI G'GATC_C
Cuts at: 2208 2208
Size: 5183
BanI G'GyrC_C
Cuts at: 2075 2199 2613 2861 2896 2075
Size: 124 414 248 35 4362
Fragments arranged by size:
4362 414 248 124 35
BanII G_rGCy'C
Cuts at: 2197 3227 2197
Size: 1030 4153
BbeI G_GCGC'C
Cuts at: 2617 2865 2617
Size: 248 4935
BbsI GAAGACnn'nnnn_
Cuts at: 2114 2114
Size: 5183
BciVI GTATCCnnnnn_n'
Cuts at: 1171 3081 3842 5183 1171
Size: 1910 761 1341 1171
Fragments arranged by size:
1910 1341 1171 761
BclI T'GATC_A
Cuts at: 2703 2703
Size: 5183
BglI GCCn_nnn'nGGC
Cuts at: 1318 1385 2144 1318
Size: 67 759 4357
Fragments arranged by size:
4357 759 67
BglII A'GATC_T
Cuts at: 1053 2698 4735 1053
Size: 1645 2037 1501
Fragments arranged by size:
2037 1645 1501
BlpI GC'TnA_GC
Cuts at: 1266 1725 2295 1266
Size: 459 570 4154
Fragments arranged by size:
4154 570 459
Bme1580I G_kGCm'C
Cuts at: 1468 2808 2901 3957 1468
Size: 1340 93 1056 2694
Fragments arranged by size:
2694 1340 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2395 2476 2811 4955 2395
Size: 81 335 2144 2623
Fragments arranged by size:
2623 2144 335 81
BmtI G_CTAG'C
Cuts at: 916 916
Size: 5183
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1006 1863 2069 3550 1006
Size: 857 206 1481 2639
Fragments arranged by size:
2639 1481 857 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1016 1016
Size: 5183
Bpu10I CC'TnA_GC
Cuts at: 569 569
Size: 5183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 2153 2359 3238 3730 4028 395
Size: 1758 206 879 492 298 1550
Fragments arranged by size:
1758 1550 879 492 298 206
BsaI GGTCTCn'nnnn_
Cuts at: 261 4887 261
Size: 4626 557
BsaAI yAC'GTr
Cuts at: 2536 2536
Size: 5183
BsaBI GATnn'nnATC
Cuts at: 6 2697 4734 6
Size: 2691 2037 455
Fragments arranged by size:
2691 2037 455
BsaHI Gr'CG_yC
Cuts at: 2614 2862 3564 5060 2614
Size: 248 702 1496 2737
Fragments arranged by size:
2737 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2372 2893 3571 3845 3992 4403 2372
Size: 521 678 274 147 411 3152
Fragments arranged by size:
3152 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1936 1966 1936
Size: 30 5153
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1747 1747
Size: 5183
BseYI C'CCAG_C
Cuts at: 1065 1618 1649 2608 3366 3943 4439 1065
Size: 553 31 959 758 577 496 1809
Fragments arranged by size:
1809 959 758 577 553 496 31
BsiEI CG_ry'CG
Cuts at: 104 2725 2771 3979 104
Size: 2621 46 1208 1308
Fragments arranged by size:
2621 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1468 2197 2975 3165 3957 1468
Size: 729 778 190 792 2694
Fragments arranged by size:
2694 792 778 729 190
Bsp1286I G_dGCh'C
Cuts at: 1468 2197 2808 2901 2975 3165 3227 3957 1468
Size: 729 611 93 74 190 62 730 2694
Fragments arranged by size:
2694 730 729 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4403 4403
Size: 5183
BspHI T'CATG_A
Cuts at: 4359 4359
Size: 5183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2233 2447 2749 3130 2233
Size: 214 302 381 4286
Fragments arranged by size:
4286 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1724 1952 3475 3529 5013 5178 50
Size: 1674 228 1523 54 1484 165 55
Fragments arranged by size:
1674 1523 1484 228 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3095 3095
Size: 5183
BsrFI r'CCGG_y
Cuts at: 247 3181 3362 3571 247
Size: 2934 181 209 1859
Fragments arranged by size:
2934 1859 209 181
BsrGI T'GTAC_A
Cuts at: 285 285
Size: 5183
BssSI C'ACGA_G
Cuts at: 991 3454 3812 991
Size: 2463 358 2362
Fragments arranged by size:
2463 2362 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1150 2560 1150
Size: 1410 3773
BstBI TT'CG_AA
Cuts at: 3544 3544
Size: 5183
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2035
Size: 5183
BstYI r'GATC_y
Cuts at: 1 327 685 1053 1402 1428 2208 2675 2698
Size: 326 358 368 349 26 780 467 23
Cuts at: 2698 3033 3279 4291 4377 4389 4735 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 780 467 449 368 358 349 346
335 326 246 86 26 23 12
BstZ17I GTA'TAC
Cuts at: 427 427
Size: 5183
BtgI C'CryG_G
Cuts at: 107 107
Size: 5183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3133 3314 3477 3133
Size: 181 163 4839
Fragments arranged by size:
4839 181 163
BtsI GCAGTG_nn'
Cuts at: 1260 2426 1260
Size: 1166 4017
DraI TTT'AAA
Cuts at: 770 2182 770
Size: 1412 3771
DrdI GACnn_nn'nnGTC
Cuts at: 2889 3747 2889
Size: 858 4325
EaeI y'GGCC_r
Cuts at: 2768 2942 3333 3360 4705 2768
Size: 174 391 27 1345 3246
Fragments arranged by size:
3246 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2768 2768
Size: 5183
EarI CTCTTCn'nnn_
Cuts at: 1395 3206 3416 1395
Size: 1811 210 3162
Fragments arranged by size:
3162 1811 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 975 3391 3701 3847 4993 975
Size: 2416 310 146 1146 1165
Fragments arranged by size:
2416 1165 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2195 2195
Size: 5183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 1016 1060 1292 3008 3440 4187 4455 152
Size: 864 44 232 1716 432 747 268 880
Fragments arranged by size:
1716 880 864 747 432 268 232 44
EcoNI CCTnn'n_nnAGG
Cuts at: 188 188
Size: 5183
EcoO109I rG'GnC_Cy
Cuts at: 987 2322 987
Size: 1335 3848
EcoRI G'AATT_C
Cuts at: 438 1446 2187 438
Size: 1008 741 3434
Fragments arranged by size:
3434 1008 741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3202 3202
Size: 5183
FspI TGC'GCA
Cuts at: 2964 2964
Size: 5183
HaeII r_GCGC'y
Cuts at: 2617 2865 3887 4866 2617
Size: 248 1022 979 2934
Fragments arranged by size:
2934 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 455 487 1209 1241 3025 3055 3057 3087 455
Size: 32 722 32 1784 30 2 30 2551
Fragments arranged by size:
2551 1784 722 32 32 30 30 2
HincII GTy'rAC
Cuts at: 563 716 2222 563
Size: 153 1506 3524
Fragments arranged by size:
3524 1506 153
HpaI GTT'AAC
Cuts at: 716 716
Size: 5183
KasI G'GCGC_C
Cuts at: 2613 2861 2613
Size: 248 4935
KpnI G_GTAC'C
Cuts at: 2079 2203 2079
Size: 124 5059
MfeI C'AATT_G
Cuts at: 1154 1196 1154
Size: 42 5141
MluI A'CGCG_T
Cuts at: 4616 4616
Size: 5183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1777 3854 4038 1777
Size: 2077 184 2922
Fragments arranged by size:
2922 2077 184
MscI TGG'CCA
Cuts at: 2944 2944
Size: 5183
MslI CAynn'nnrTG
Cuts at: 3172 3299 3172
Size: 127 5056
MspA1I CmG'CkG
Cuts at: 1309 1538 1779 2294 2608 2968 3981 4226 1309
Size: 229 241 515 314 360 1013 245 2266
Fragments arranged by size:
2266 1013 515 360 314 245 241 229
NaeI GCC'GGC
Cuts at: 3364 3364
Size: 5183
NarI GG'CG_CC
Cuts at: 2614 2862 2614
Size: 248 4935
NcoI C'CATG_G
Cuts at: 107 107
Size: 5183
NdeI CA'TA_TG
Cuts at: 162 162
Size: 5183
NgoMIV G'CCGG_C
Cuts at: 3362 3362
Size: 5183
NheI G'CTAG_C
Cuts at: 912 912
Size: 5183
NspI r_CATG'y
Cuts at: 218 384 504 1014 1306 2236 2736 3643 218
Size: 166 120 510 292 930 500 907 1758
Fragments arranged by size:
1758 930 907 510 500 292 166 120
PciI A'CATG_T
Cuts at: 214 500 3639 214
Size: 286 3139 1758
Fragments arranged by size:
3139 1758 286
PflMI CCAn_nnn'nTGG
Cuts at: 1115 1115
Size: 5183
PmeI GTTT'AAAC
Cuts at: 2182 2182
Size: 5183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 4322
Size: 32 5151
PpuMI rG'GwC_Cy
Cuts at: 987 987
Size: 5183
PstI C_TGCA'G
Cuts at: 2230 2915 2230
Size: 685 4498
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5183
PvuII CAG'CTG
Cuts at: 1309 1779 2608 2968 1309
Size: 470 829 360 3524
Fragments arranged by size:
3524 829 470 360
RsrII CG'GwC_CG
Cuts at: 3378 3378
Size: 5183
SacI G_AGCT'C
Cuts at: 2197 2197
Size: 5183
SalI G'TCGA_C
Cuts at: 2220 2220
Size: 5183
SapI GCTCTTCn'nnn_
Cuts at: 1395 3206 3416 1395
Size: 1811 210 3162
Fragments arranged by size:
3162 1811 210
SbfI CC_TGCA'GG
Cuts at: 2230 2230
Size: 5183
ScaI AGT'ACT
Cuts at: 4700 4700
Size: 5183
SfcI C'TryA_G
Cuts at: 33 863 905 2226 2911 3904 4095 33
Size: 830 42 1321 685 993 191 1121
Fragments arranged by size:
1321 1121 993 830 685 191 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 2144 2144
Size: 5183
SfoI GGC'GCC
Cuts at: 2615 2863 2615
Size: 248 4935
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5183
SmaI CCC'GGG
Cuts at: 2205 2205
Size: 5183
SmlI C'TyrA_G
Cuts at: 410 2041 2168 2338 3253 3745 4007 410
Size: 1631 127 170 915 492 262 1586
Fragments arranged by size:
1631 1586 915 492 262 170 127
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5183
SphI G_CATG'C
Cuts at: 384 2236 2736 384
Size: 1852 500 2831
Fragments arranged by size:
2831 1852 500
SspI AAT'ATT
Cuts at: 1767 1767
Size: 5183
StuI AGG'CCT
Cuts at: 1532 1532
Size: 5183
StyI C'CwwG_G
Cuts at: 107 2317 2671 107
Size: 2210 354 2619
Fragments arranged by size:
2619 2210 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1410 2766 2899 3358 3567 1410
Size: 1356 133 459 209 3026
Fragments arranged by size:
3026 1356 459 209 133
TatI w'GTAC_w
Cuts at: 285 4698 285
Size: 4413 770
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1791 1823 4700 4732 1791
Size: 32 2877 32 2242
Fragments arranged by size:
2877 2242 32 32
Tth111I GACn'n_nGTC
Cuts at: 2980 2980
Size: 5183
XbaI T'CTAG_A
Cuts at: 1557 1981 2214 1557
Size: 424 233 4526
Fragments arranged by size:
4526 424 233
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1229 2678 1229
Size: 1449 3734
XmaI C'CCGG_G
Cuts at: 2203 2203
Size: 5183
XmnI GAAnn'nnTTC
Cuts at: 116 116
Size: 5183
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BsgI
BsiWI BsmI BsmBI BssHII BstEII Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SwaI
XhoI ZraI