Restriction map for
pF1KB9422
(Circular) (Six-base) MAPSORT of: pF1KB9422.seq Check: 2596 from: 1 to: 4826
pF1KB9422 4826 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1864 1864
Size: 4826
Acc65I G'GTAC_C
Cuts at: 1842 1842
Size: 4826
AclI AA'CG_TT
Cuts at: 4666 4666
Size: 4826
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 1378 2651 3083 3830 4098 680
Size: 698 1273 432 747 268 1408
Fragments arranged by size:
1408 1273 747 698 432 268
AflIII A'CryG_T
Cuts at: 206 1787 2178 3282 4259 206
Size: 1581 391 1104 977 773
Fragments arranged by size:
1581 1104 977 773 391
AgeI A'CCGG_T
Cuts at: 3214 3214
Size: 4826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1457 1457
Size: 4826
AlwNI CAG_nnn'CTG
Cuts at: 798 1718 2131 3698 798
Size: 920 413 1567 1926
Fragments arranged by size:
1926 1567 920 413
ApaI G_GGCC'C
Cuts at: 710 710
Size: 4826
ApaLI G'TGCA_C
Cuts at: 3596 3596
Size: 4826
ApoI r'AATT_y
Cuts at: 1110 1541 1830 4179 4435 1110
Size: 431 289 2349 256 1501
Fragments arranged by size:
2349 1501 431 289 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4826
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4826
AvaI C'yCGr_G
Cuts at: 1846 1846
Size: 4826
BamHI G'GATC_C
Cuts at: 1851 1851
Size: 4826
BanI G'GyrC_C
Cuts at: 1842 2256 2504 2539 1842
Size: 414 248 35 4129
Fragments arranged by size:
4129 414 248 35
BanII G_rGCy'C
Cuts at: 248 710 1488 1840 2870 248
Size: 462 778 352 1030 2204
Fragments arranged by size:
2204 1030 778 462 352
BbeI G_GCGC'C
Cuts at: 2260 2508 2260
Size: 248 4578
BbsI GAAGACnn'nnnn_
Cuts at: 1616 1616
Size: 4826
BbvCI CC'TCA_GC
Cuts at: 1706 1706
Size: 4826
BciVI GTATCCnnnnn_n'
Cuts at: 2724 3485 4826 2724
Size: 761 1341 2724
Fragments arranged by size:
2724 1341 761
BclI T'GATC_A
Cuts at: 2346 2346
Size: 4826
BfrBI ATG'CAT
Cuts at: 1297 1297
Size: 4826
BglII A'GATC_T
Cuts at: 739 2341 4378 739
Size: 1602 2037 1187
Fragments arranged by size:
2037 1602 1187
BlpI GC'TnA_GC
Cuts at: 1058 1938 1058
Size: 880 3946
Bme1580I G_kGCm'C
Cuts at: 710 2451 2544 3600 710
Size: 1741 93 1056 1936
Fragments arranged by size:
1936 1741 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 149 2038 2119 2454 4598 149
Size: 1889 81 335 2144 377
Fragments arranged by size:
2144 1889 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3193 3193
Size: 4826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 600 985 477
Size: 123 385 4318
Fragments arranged by size:
4318 385 123
Bpu10I CC'TnA_GC
Cuts at: 535 1706 535
Size: 1171 3655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 589 835 2002 2881 3373 3671 426
Size: 163 246 1167 879 492 298 1581
Fragments arranged by size:
1581 1167 879 492 298 246 163
BsaI GGTCTCn'nnnn_
Cuts at: 4530 4530
Size: 4826
BsaAI yAC'GTr
Cuts at: 1803 2179 1803
Size: 376 4450
BsaBI GATnn'nnATC
Cuts at: 6 2340 4377 6
Size: 2334 2037 455
Fragments arranged by size:
2334 2037 455
BsaHI Gr'CG_yC
Cuts at: 2257 2505 3207 4703 2257
Size: 248 702 1496 2380
Fragments arranged by size:
2380 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2015 2536 3214 3488 3635 4046 2015
Size: 521 678 274 147 411 2795
Fragments arranged by size:
2795 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 983 1013 983
Size: 30 4796
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 331 615 790 1715 235
Size: 96 284 175 925 3346
Fragments arranged by size:
3346 925 284 175 96
BseYI C'CCAG_C
Cuts at: 2251 3009 3586 4082 2251
Size: 758 577 496 2995
Fragments arranged by size:
2995 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2368 2414 3622 104
Size: 2264 46 1208 1308
Fragments arranged by size:
2264 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 248 934 1840 2618 2808 3600 248
Size: 686 906 778 190 792 1474
Fragments arranged by size:
1474 906 792 778 686 190
Bsp1286I G_dGCh'C
Cuts at: 248 710 934 1488 1840 2451 2544 2618 2808
Size: 462 224 554 352 611 93 74 190
Cuts at: 2808 2870 3600 248
Size: 62 730 1474
Fragments arranged by size:
1474 730 611 554 462 352 224 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4046 4046
Size: 4826
BspHI T'CATG_A
Cuts at: 411 1545 4002 411
Size: 1134 2457 1235
Fragments arranged by size:
2457 1235 1134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1876 2090 2392 2773 1876
Size: 214 302 381 3929
Fragments arranged by size:
3929 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3118 3172 4656 4821 50
Size: 3068 54 1484 165 55
Fragments arranged by size:
3068 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1439 1567 2738 1439
Size: 128 1171 3527
Fragments arranged by size:
3527 1171 128
BsrFI r'CCGG_y
Cuts at: 2824 3005 3214 2824
Size: 181 209 4436
Fragments arranged by size:
4436 209 181
BssSI C'ACGA_G
Cuts at: 711 732 3097 3455 711
Size: 21 2365 358 2082
Fragments arranged by size:
2365 2082 358 21
BstAPI GCAn_nnn'nTGC
Cuts at: 2203 2203
Size: 4826
BstBI TT'CG_AA
Cuts at: 767 1607 3187 767
Size: 840 1580 2406
Fragments arranged by size:
2406 1580 840
BstYI r'GATC_y
Cuts at: 1 739 1851 2318 2341 2676 2922 3934 4020
Size: 738 1112 467 23 335 246 1012 86
Cuts at: 4020 4032 4378 1
Size: 12 346 449
Fragments arranged by size:
1112 1012 738 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 808 1480 808
Size: 672 4154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2776 2957 3120 2776
Size: 181 163 4482
Fragments arranged by size:
4482 181 163
BtsI GCAGTG_nn'
Cuts at: 1358 2069 1358
Size: 711 4115
DraI TTT'AAA
Cuts at: 1825 1825
Size: 4826
DrdI GACnn_nn'nnGTC
Cuts at: 2532 3390 2532
Size: 858 3968
EaeI y'GGCC_r
Cuts at: 2411 2585 2976 3003 4348 2411
Size: 174 391 27 1345 2889
Fragments arranged by size:
2889 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2411 2411
Size: 4826
EarI CTCTTCn'nnn_
Cuts at: 428 947 1066 1448 2849 3059 428
Size: 519 119 382 1401 210 2195
Fragments arranged by size:
2195 1401 519 382 210 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3034 3344 3490 4636 3034
Size: 310 146 1146 3224
Fragments arranged by size:
3224 1146 310 146
EcoICRI GAG'CTC
Cuts at: 246 1838 246
Size: 1592 3234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 600 680 985 1378 2651 3083 3830 4098
Size: 123 80 305 393 1273 432 747 268
Cuts at: 4098 477
Size: 1205
Fragments arranged by size:
1273 1205 747 432 393 305 268 123
80
EcoO109I rG'GnC_Cy
Cuts at: 477 539 1095 1458 1965 477
Size: 62 556 363 507 3338
Fragments arranged by size:
3338 556 507 363 62
EcoRI G'AATT_C
Cuts at: 1830 1830
Size: 4826
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 780 2845 780
Size: 2065 2761
FspI TGC'GCA
Cuts at: 2607 2607
Size: 4826
HaeII r_GCGC'y
Cuts at: 2260 2508 3530 4509 2260
Size: 248 1022 979 2577
Fragments arranged by size:
2577 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1222 1254 1629 1661 2668 2698 2700 2730 1222
Size: 32 375 32 1007 30 2 30 3318
Fragments arranged by size:
3318 1007 375 32 32 30 30 2
HincII GTy'rAC
Cuts at: 782 1865 782
Size: 1083 3743
HindIII A'AGCT_T
Cuts at: 1273 1273
Size: 4826
HpaI GTT'AAC
Cuts at: 782 782
Size: 4826
KasI G'GCGC_C
Cuts at: 2256 2504 2256
Size: 248 4578
KpnI G_GTAC'C
Cuts at: 1846 1846
Size: 4826
MfeI C'AATT_G
Cuts at: 1307 1307
Size: 4826
MluI A'CGCG_T
Cuts at: 4259 4259
Size: 4826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 954 3497 3681 954
Size: 2543 184 2099
Fragments arranged by size:
2543 2099 184
MscI TGG'CCA
Cuts at: 2587 2587
Size: 4826
MslI CAynn'nnrTG
Cuts at: 416 1292 2815 2942 416
Size: 876 1523 127 2300
Fragments arranged by size:
2300 1523 876 127
MspA1I CmG'CkG
Cuts at: 128 267 620 1413 1575 1731 1937 2251 2611
Size: 139 353 793 162 156 206 314 360
Cuts at: 2611 3624 3869 128
Size: 1013 245 1085
Fragments arranged by size:
1085 1013 793 360 353 314 245 206
162 156 139
NaeI GCC'GGC
Cuts at: 3007 3007
Size: 4826
NarI GG'CG_CC
Cuts at: 2257 2505 2257
Size: 248 4578
NgoMIV G'CCGG_C
Cuts at: 3005 3005
Size: 4826
NsiI A_TGCA'T
Cuts at: 1299 1299
Size: 4826
NspI r_CATG'y
Cuts at: 1194 1879 2379 3286 1194
Size: 685 500 907 2734
Fragments arranged by size:
2734 907 685 500
PasI CC'CwG_GG
Cuts at: 503 503
Size: 4826
PciI A'CATG_T
Cuts at: 3282 3282
Size: 4826
PmeI GTTT'AAAC
Cuts at: 1825 1825
Size: 4826
PmlI CAC'GTG
Cuts at: 1803 1803
Size: 4826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 587 619 3965 3997 587
Size: 32 3346 32 1416
Fragments arranged by size:
3346 1416 32 32
PpuMI rG'GwC_Cy
Cuts at: 477 539 1458 477
Size: 62 919 3845
Fragments arranged by size:
3845 919 62
PspOMI G'GGCC_C
Cuts at: 706 706
Size: 4826
PstI C_TGCA'G
Cuts at: 233 602 747 1873 2558 233
Size: 369 145 1126 685 2501
Fragments arranged by size:
2501 1126 685 369 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4826
PvuII CAG'CTG
Cuts at: 128 267 1413 1575 2251 2611 128
Size: 139 1146 162 676 360 2343
Fragments arranged by size:
2343 1146 676 360 162 139
RsrII CG'GwC_CG
Cuts at: 3021 3021
Size: 4826
SacI G_AGCT'C
Cuts at: 248 1840 248
Size: 1592 3234
SalI G'TCGA_C
Cuts at: 1863 1863
Size: 4826
SapI GCTCTTCn'nnn_
Cuts at: 428 2849 3059 428
Size: 2421 210 2195
Fragments arranged by size:
2421 2195 210
SbfI CC_TGCA'GG
Cuts at: 1873 1873
Size: 4826
ScaI AGT'ACT
Cuts at: 4343 4343
Size: 4826
SfcI C'TryA_G
Cuts at: 33 229 598 743 1255 1869 2554 3547 3738
Size: 196 369 145 512 614 685 993 191
Cuts at: 3738 33
Size: 1121
Fragments arranged by size:
1121 993 685 614 512 369 196 191
145
SfoI GGC'GCC
Cuts at: 2258 2506 2258
Size: 248 4578
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4826
SmaI CCC'GGG
Cuts at: 1848 1848
Size: 4826
SmlI C'TyrA_G
Cuts at: 405 604 850 1981 2896 3388 3650 405
Size: 199 246 1131 915 492 262 1581
Fragments arranged by size:
1581 1131 915 492 262 246 199
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4826
SphI G_CATG'C
Cuts at: 1194 1879 2379 1194
Size: 685 500 3641
Fragments arranged by size:
3641 685 500
SspI AAT'ATT
Cuts at: 1737 1737
Size: 4826
StyI C'CwwG_G
Cuts at: 1960 2314 1960
Size: 354 4472
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 209 268 2409 2542 3001 3210 209
Size: 59 2141 133 459 209 1825
Fragments arranged by size:
2141 1825 459 209 133 59
TatI w'GTAC_w
Cuts at: 4341 4341
Size: 4826
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 968 983 1000 1015 4343 4375 968
Size: 15 17 15 3328 32 1419
Fragments arranged by size:
3328 1419 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 2623 2623
Size: 4826
XbaI T'CTAG_A
Cuts at: 1857 1857
Size: 4826
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2321 2321
Size: 4826
XmaI C'CCGG_G
Cuts at: 1846 1846
Size: 4826
XmnI GAAnn'nnTTC
Cuts at: 763 763
Size: 4826
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PasI PciI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI NcoI
NdeI NheI NotI NruI PacI PflMI PfoI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI