Restriction map for
pF1KB9423
(Circular) (Six-base) MAPSORT of: pF1KB9423.seq Check: 639 from: 1 to: 3764
pF1KB9423 3764 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 670 670
Size: 3764
AccI GT'mk_AC
Cuts at: 605 802 605
Size: 197 3567
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 524 3604 524
Size: 3080 684
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 1589 2021 2768 3036 145
Size: 1444 432 747 268 873
Fragments arranged by size:
1444 873 747 432 268
AfeI AGC'GCT
Cuts at: 190 190
Size: 3764
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 1069 2636 1069
Size: 1567 2197
ApaI G_GGCC'C
Cuts at: 391 391
Size: 3764
ApaLI G'TGCA_C
Cuts at: 598 2534 598
Size: 1936 1828
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 264 784 264
Size: 520 3244
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 181 337 780 1194 1442 1477 181
Size: 156 443 414 248 35 2468
Fragments arranged by size:
2468 443 414 248 156 35
BanII G_rGCy'C
Cuts at: 391 643 778 1808 391
Size: 252 135 1030 2347
Fragments arranged by size:
2347 1030 252 135
BbeI G_GCGC'C
Cuts at: 1198 1446 1198
Size: 248 3516
BbsI GAAGACnn'nnnn_
Cuts at: 126 126
Size: 3764
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2423 3764 1662
Size: 761 1341 1662
Fragments arranged by size:
1662 1341 761
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglII A'GATC_T
Cuts at: 1279 3316 1279
Size: 2037 1727
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 184 391 602 1389 1482 2538 184
Size: 207 211 787 93 1056 1410
Fragments arranged by size:
1410 1056 787 211 207 93
BmgBI CAC'GTC
Cuts at: 603 603
Size: 3764
BmrI ACTGGGnnnn_n'
Cuts at: 294 976 1057 1392 3536 294
Size: 682 81 335 2144 522
Fragments arranged by size:
2144 682 522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 742
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1819 2311 2609 940
Size: 879 492 298 2095
Fragments arranged by size:
2095 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 300 654 1117 300
Size: 354 463 2947
Fragments arranged by size:
2947 463 354
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 217 1195 1443 2145 3641 217
Size: 978 248 702 1496 340
Fragments arranged by size:
1496 978 702 340 248
BsaWI w'CCGG_w
Cuts at: 428 953 1474 2152 2426 2573 2984 428
Size: 525 521 678 274 147 411 1208
Fragments arranged by size:
1208 678 525 521 411 274 147
BseYI C'CCAG_C
Cuts at: 185 1189 1947 2524 3020 185
Size: 1004 758 577 496 929
Fragments arranged by size:
1004 929 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 274 339 184
Size: 90 65 3609
Fragments arranged by size:
3609 90 65
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 602 778 1556 1746 2538 602
Size: 176 778 190 792 1828
Fragments arranged by size:
1828 792 778 190 176
Bsp1286I G_dGCh'C
Cuts at: 184 391 602 643 778 1389 1482 1556 1746
Size: 207 211 41 135 611 93 74 190
Cuts at: 1746 1808 2538 184
Size: 62 730 1410
Fragments arranged by size:
1410 730 611 211 207 190 135 93
74 62 41
BspEI T'CCGG_A
Cuts at: 428 2984 428
Size: 2556 1208
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 248 476 670 814 1028 1330 1711 248
Size: 228 194 144 214 302 381 2301
Fragments arranged by size:
2301 381 302 228 214 194 144
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 1762 1943 2152 1762
Size: 181 209 3374
Fragments arranged by size:
3374 209 181
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 561 1141 561
Size: 580 3184
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstXI CCAn_nnnn'nTGG
Cuts at: 462 720 462
Size: 258 3506
BstYI r'GATC_y
Cuts at: 1 569 789 1256 1279 1614 1860 2872 2958
Size: 568 220 467 23 335 246 1012 86
Cuts at: 2958 2970 3316 1
Size: 12 346 449
Fragments arranged by size:
1012 568 467 449 346 335 246 220
86 23 12
BtgI C'CryG_G
Cuts at: 437 437
Size: 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 612 1714 1895 2058 612
Size: 1102 181 163 2318
Fragments arranged by size:
2318 1102 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1007
Size: 3764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 507 542 507
Size: 35 3729
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DraIII CAC_nnn'GTG
Cuts at: 303 303
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 248 1349 1523 1914 1941 3286 248
Size: 1101 174 391 27 1345 726
Fragments arranged by size:
1345 1101 726 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 517 1787 1997 517
Size: 1270 210 2284
Fragments arranged by size:
2284 1270 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 1972 2282 2428 3574 145
Size: 1827 310 146 1146 335
Fragments arranged by size:
1827 1146 335 310 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 742 1589 2021 2768 3036 145
Size: 597 847 432 747 268 873
Fragments arranged by size:
873 847 747 597 432 268
EcoO109I rG'GnC_Cy
Cuts at: 273 387 388 507 903 273
Size: 114 1 119 396 3134
Fragments arranged by size:
3134 396 119 114 1
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 150 1783 150
Size: 1633 2131
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 192 1198 1446 2468 3447 192
Size: 1006 248 1022 979 509
Fragments arranged by size:
1022 1006 979 509 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1606 1636 1638 1668 1606
Size: 30 2 30 3702
Fragments arranged by size:
3702 30 30 2
HincII GTy'rAC
Cuts at: 324 803 324
Size: 479 3285
KasI G'GCGC_C
Cuts at: 1194 1442 1194
Size: 248 3516
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 137 2435 2619 137
Size: 2298 184 1282
Fragments arranged by size:
2298 1282 184
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 1753 1880 1753
Size: 127 3637
MspA1I CmG'CkG
Cuts at: 258 407 875 1189 1549 2562 2807 258
Size: 149 468 314 360 1013 245 1215
Fragments arranged by size:
1215 1013 468 360 314 245 149
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 1195 1443 1195
Size: 248 3516
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NspI r_CATG'y
Cuts at: 817 1317 2224 817
Size: 500 907 2357
Fragments arranged by size:
2357 907 500
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PmlI CAC'GTG
Cuts at: 300 654 300
Size: 354 3410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 409 441 2903 2935 409
Size: 32 2462 32 1238
Fragments arranged by size:
2462 1238 32 32
PpuMI rG'GwC_Cy
Cuts at: 273 507 273
Size: 234 3530
PspOMI G'GGCC_C
Cuts at: 387 387
Size: 3764
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 649 681 649
Size: 32 3732
PstI C_TGCA'G
Cuts at: 557 811 1496 557
Size: 254 685 2825
Fragments arranged by size:
2825 685 254
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 258 1189 1549 258
Size: 931 360 2473
Fragments arranged by size:
2473 931 360
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 553 807 1492 2485 2676 33
Size: 520 254 685 993 191 1121
Fragments arranged by size:
1121 993 685 520 254 191
SfoI GGC'GCC
Cuts at: 1196 1444 1196
Size: 248 3516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 266 786 266
Size: 520 3244
SmlI C'TyrA_G
Cuts at: 919 1834 2326 2588 919
Size: 915 492 262 2095
Fragments arranged by size:
2095 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 713 898 1252 713
Size: 185 354 3225
Fragments arranged by size:
3225 354 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 199 276 1347 1480 1939 2148 199
Size: 77 1071 133 459 209 1815
Fragments arranged by size:
1815 1071 459 209 133 77
TatI w'GTAC_w
Cuts at: 3279 3279
Size: 3764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 522 554 3281 3313 522
Size: 32 2727 32 973
Fragments arranged by size:
2727 973 32 32
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1259 1259
Size: 3764
XmaI C'CCGG_G
Cuts at: 264 784 264
Size: 520 3244
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PshAI PsiI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI