Restriction map for
pF1KB9425
(Circular) (Six-base) MAPSORT of: pF1KB9425.seq Check: 6129 from: 1 to: 5324
pF1KB9425 5324 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 730 730
Size: 5324
AatII G_ACGT'C
Cuts at: 1436 2192 1436
Size: 756 4568
AccI GT'mk_AC
Cuts at: 2362 2362
Size: 5324
Acc65I G'GTAC_C
Cuts at: 2340 2340
Size: 5324
AclI AA'CG_TT
Cuts at: 5164 5164
Size: 5324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1426 3149 3581 4328 4596 1426
Size: 1723 432 747 268 2154
Fragments arranged by size:
2154 1723 747 432 268
AfeI AGC'GCT
Cuts at: 1606 1606
Size: 5324
AflII C'TTAA_G
Cuts at: 130 130
Size: 5324
AflIII A'CryG_T
Cuts at: 2676 3780 4757 2676
Size: 1104 977 3243
Fragments arranged by size:
3243 1104 977
AgeI A'CCGG_T
Cuts at: 3712 3712
Size: 5324
AleI CACnn'nnGTG
Cuts at: 322 724 2239 322
Size: 402 1515 3407
Fragments arranged by size:
3407 1515 402
AlwNI CAG_nnn'CTG
Cuts at: 2629 4196 2629
Size: 1567 3757
ApaI G_GGCC'C
Cuts at: 1884 1884
Size: 5324
ApaLI G'TGCA_C
Cuts at: 4094 4094
Size: 5324
ApoI r'AATT_y
Cuts at: 1615 2278 2328 4677 4933 1615
Size: 663 50 2349 256 2006
Fragments arranged by size:
2349 2006 663 256 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 5324
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5324
AvaI C'yCGr_G
Cuts at: 2216 2344 2216
Size: 128 5196
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 693 726 1492 1525 693
Size: 33 766 33 4492
Fragments arranged by size:
4492 766 33 33
BamHI G'GATC_C
Cuts at: 2349 2349
Size: 5324
BanI G'GyrC_C
Cuts at: 740 1084 2340 2754 3002 3037 740
Size: 344 1256 414 248 35 3027
Fragments arranged by size:
3027 1256 414 344 248 35
BanII G_rGCy'C
Cuts at: 752 808 1273 1884 2338 3368 752
Size: 56 465 611 454 1030 2708
Fragments arranged by size:
2708 1030 611 465 454 56
BbeI G_GCGC'C
Cuts at: 2758 3006 2758
Size: 248 5076
BbsI GAAGACnn'nnnn_
Cuts at: 289 2312 289
Size: 2023 3301
BciVI GTATCCnnnnn_n'
Cuts at: 694 856 3222 3983 5324 694
Size: 162 2366 761 1341 694
Fragments arranged by size:
2366 1341 761 694 162
BclI T'GATC_A
Cuts at: 488 2844 488
Size: 2356 2968
BglII A'GATC_T
Cuts at: 2839 4876 2839
Size: 2037 3287
BlpI GC'TnA_GC
Cuts at: 1996 2436 1996
Size: 440 4884
Bme1580I G_kGCm'C
Cuts at: 1884 2949 3042 4098 1884
Size: 1065 93 1056 3110
Fragments arranged by size:
3110 1065 1056 93
BmgBI CAC'GTC
Cuts at: 1455 1977 1455
Size: 522 4802
BmrI ACTGGGnnnn_n'
Cuts at: 1981 2133 2536 2617 2952 5096 1981
Size: 152 403 81 335 2144 2209
Fragments arranged by size:
2209 2144 403 335 152 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3691 3691
Size: 5324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 2155 484
Size: 1671 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 1372 2500 3379 3871 4169 1204
Size: 168 1128 879 492 298 2359
Fragments arranged by size:
2359 1128 879 492 298 168
BsaI GGTCTCn'nnnn_
Cuts at: 781 955 1577 5028 781
Size: 174 622 3451 1077
Fragments arranged by size:
3451 1077 622 174
BsaAI yAC'GTr
Cuts at: 2677 2677
Size: 5324
BsaBI GATnn'nnATC
Cuts at: 6 139 2838 4875 6
Size: 133 2699 2037 455
Fragments arranged by size:
2699 2037 455 133
BsaHI Gr'CG_yC
Cuts at: 1433 2087 2189 2755 3003 3705 5201 1433
Size: 654 102 566 248 702 1496 1556
Fragments arranged by size:
1556 1496 702 654 566 248 102
BsaWI w'CCGG_w
Cuts at: 2513 3034 3712 3986 4133 4544 2513
Size: 521 678 274 147 411 3293
Fragments arranged by size:
3293 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1840 1870 1840
Size: 30 5294
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 430 1238 1243 430
Size: 808 5 4511
Fragments arranged by size:
4511 808 5
BseYI C'CCAG_C
Cuts at: 769 809 2749 3507 4084 4580 769
Size: 40 1940 758 577 496 1513
Fragments arranged by size:
1940 1513 758 577 496 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 702 1446 384
Size: 318 744 4262
Fragments arranged by size:
4262 744 318
BsiEI CG_ry'CG
Cuts at: 104 205 565 2866 2912 4120 104
Size: 101 360 2301 46 1208 1308
Fragments arranged by size:
2301 1308 1208 360 101 46
BsiHKAI G_wGCw'C
Cuts at: 752 1235 2132 2338 3116 3306 4098 752
Size: 483 897 206 778 190 792 1978
Fragments arranged by size:
1978 897 792 778 483 206 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1253 2180 1253
Size: 927 4397
Bsp1286I G_dGCh'C
Cuts at: 752 808 1235 1273 1884 2132 2338 2949 3042
Size: 56 427 38 611 248 206 611 93
Cuts at: 3042 3116 3306 3368 4098 752
Size: 74 190 62 730 1978
Fragments arranged by size:
1978 730 611 611 427 248 206 190
93 74 62 56 38
BspEI T'CCGG_A
Cuts at: 4544 4544
Size: 5324
BspHI T'CATG_A
Cuts at: 2030 4500 2030
Size: 2470 2854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 730 2374 2588 2890 3271 730
Size: 1644 214 302 381 2783
Fragments arranged by size:
2783 1644 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3616 3670 5154 5319 50
Size: 3566 54 1484 165 55
Fragments arranged by size:
3566 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 230 660 1076 3236 230
Size: 430 416 2160 2318
Fragments arranged by size:
2318 2160 430 416
BsrFI r'CCGG_y
Cuts at: 2226 3322 3503 3712 2226
Size: 1096 181 209 3838
Fragments arranged by size:
3838 1096 209 181
BssSI C'ACGA_G
Cuts at: 745 3595 3953 745
Size: 2850 358 2116
Fragments arranged by size:
2850 2116 358
BstAPI GCAn_nnn'nTGC
Cuts at: 918 2701 918
Size: 1783 3541
BstBI TT'CG_AA
Cuts at: 3685 3685
Size: 5324
BstXI CCAn_nnnn'nTGG
Cuts at: 1014 1314 1684 1014
Size: 300 370 4654
Fragments arranged by size:
4654 370 300
BstYI r'GATC_y
Cuts at: 1 1838 2349 2816 2839 3174 3420 4432 4518
Size: 1837 511 467 23 335 246 1012 86
Cuts at: 4518 4530 4876 1
Size: 12 346 449
Fragments arranged by size:
1837 1012 511 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1868 107
Size: 1761 3563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2054 3274 3455 3618 2054
Size: 1220 181 163 3760
Fragments arranged by size:
3760 1220 181 163
BtsI GCAGTG_nn'
Cuts at: 928 1740 2567 928
Size: 812 827 3685
Fragments arranged by size:
3685 827 812
ClaI AT'CG_AT
Cuts at: 494 1134 494
Size: 640 4684
DraI TTT'AAA
Cuts at: 2323 2323
Size: 5324
DraIII CAC_nnn'GTG
Cuts at: 764 764
Size: 5324
DrdI GACnn_nn'nnGTC
Cuts at: 313 3030 3888 313
Size: 2717 858 1749
Fragments arranged by size:
2717 1749 858
EaeI y'GGCC_r
Cuts at: 202 261 269 392 562 612 626 1016 1056
Size: 59 8 123 170 50 14 390 40
Cuts at: 1056 1257 2909 3083 3474 3501 4846 202
Size: 201 1652 174 391 27 1345 680
Fragments arranged by size:
1652 1345 680 391 390 201 174 170
123 59 50 40 27 14 8
EagI C'GGCC_G
Cuts at: 202 562 2909 202
Size: 360 2347 2617
Fragments arranged by size:
2617 2347 360
EarI CTCTTCn'nnn_
Cuts at: 471 530 1471 2216 3347 3557 471
Size: 59 941 745 1131 210 2238
Fragments arranged by size:
2238 1131 941 745 210 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3532 3842 3988 5134 3532
Size: 310 146 1146 3722
Fragments arranged by size:
3722 1146 310 146
EcoICRI GAG'CTC
Cuts at: 750 2336 750
Size: 1586 3738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 1426 2155 3149 3581 4328 4596 484
Size: 942 729 994 432 747 268 1212
Fragments arranged by size:
1212 994 942 747 729 432 268
EcoO109I rG'GnC_Cy
Cuts at: 196 861 887 1881 2463 196
Size: 665 26 994 582 3057
Fragments arranged by size:
3057 994 665 582 26
EcoRI G'AATT_C
Cuts at: 2278 2328 2278
Size: 50 5274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2175 3343 2175
Size: 1168 4156
FspI TGC'GCA
Cuts at: 3105 3105
Size: 5324
HaeII r_GCGC'y
Cuts at: 1314 1608 2055 2758 3006 4028 5007 1314
Size: 294 447 703 248 1022 979 1631
Fragments arranged by size:
1631 1022 979 703 447 294 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 326 358 480 512 659 691 1473 1505 1642
Size: 32 122 32 147 32 782 32 137
Cuts at: 1642 1674 2025 2057 3166 3196 3198 3228 326
Size: 32 351 32 1109 30 2 30 2422
Fragments arranged by size:
2422 1109 782 351 147 137 122 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 609 825 2363 609
Size: 216 1538 3570
Fragments arranged by size:
3570 1538 216
HindIII A'AGCT_T
Cuts at: 2024 2024
Size: 5324
KasI G'GCGC_C
Cuts at: 2754 3002 2754
Size: 248 5076
KpnI G_GTAC'C
Cuts at: 2344 2344
Size: 5324
MfeI C'AATT_G
Cuts at: 2252 2252
Size: 5324
MluI A'CGCG_T
Cuts at: 4757 4757
Size: 5324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1832 3995 4179 1832
Size: 2163 184 2977
Fragments arranged by size:
2977 2163 184
MscI TGG'CCA
Cuts at: 394 628 1259 3085 394
Size: 234 631 1826 2633
Fragments arranged by size:
2633 1826 631 234
MslI CAynn'nnrTG
Cuts at: 112 322 724 1342 1682 2239 3313 3440 112
Size: 210 402 618 340 557 1074 127 1996
Fragments arranged by size:
1996 1074 618 557 402 340 210 127
MspA1I CmG'CkG
Cuts at: 813 1055 1263 2435 2749 3109 4122 4367 813
Size: 242 208 1172 314 360 1013 245 1770
Fragments arranged by size:
1770 1172 1013 360 314 245 242 208
NaeI GCC'GGC
Cuts at: 3505 3505
Size: 5324
NarI GG'CG_CC
Cuts at: 2755 3003 2755
Size: 248 5076
NcoI C'CATG_G
Cuts at: 107 107
Size: 5324
NgoMIV G'CCGG_C
Cuts at: 3503 3503
Size: 5324
NspI r_CATG'y
Cuts at: 561 831 2377 2877 3784 561
Size: 270 1546 500 907 2101
Fragments arranged by size:
2101 1546 907 500 270
PasI CC'CwG_GG
Cuts at: 1884 1884
Size: 5324
PciI A'CATG_T
Cuts at: 3780 3780
Size: 5324
PflMI CCAn_nnn'nTGG
Cuts at: 173 192 173
Size: 19 5305
PfoI T'CCnGG_A
Cuts at: 2132 2132
Size: 5324
PmeI GTTT'AAAC
Cuts at: 2323 2323
Size: 5324
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 529 561 4463 4495 529
Size: 32 3902 32 1358
Fragments arranged by size:
3902 1358 32 32
PpuMI rG'GwC_Cy
Cuts at: 196 861 887 196
Size: 665 26 4633
Fragments arranged by size:
4633 665 26
PspOMI G'GGCC_C
Cuts at: 1880 1880
Size: 5324
PstI C_TGCA'G
Cuts at: 428 1650 1847 2371 3056 428
Size: 1222 197 524 685 2696
Fragments arranged by size:
2696 1222 685 524 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5324
PvuII CAG'CTG
Cuts at: 1263 2749 3109 1263
Size: 1486 360 3478
Fragments arranged by size:
3478 1486 360
RsrII CG'GwC_CG
Cuts at: 3519 3519
Size: 5324
SacI G_AGCT'C
Cuts at: 752 2338 752
Size: 1586 3738
SalI G'TCGA_C
Cuts at: 2361 2361
Size: 5324
SanDI GG'GwC_CC
Cuts at: 196 887 196
Size: 691 4633
SapI GCTCTTCn'nnn_
Cuts at: 530 2216 3347 3557 530
Size: 1686 1131 210 2297
Fragments arranged by size:
2297 1686 1131 210
SbfI CC_TGCA'GG
Cuts at: 1847 2371 1847
Size: 524 4800
ScaI AGT'ACT
Cuts at: 1013 4841 1013
Size: 3828 1496
SexAI A'CCwGG_T
Cuts at: 797 797
Size: 5324
SfcI C'TryA_G
Cuts at: 33 424 1646 1695 1843 2367 3052 4045 4236
Size: 391 1222 49 148 524 685 993 191
Cuts at: 4236 33
Size: 1121
Fragments arranged by size:
1222 1121 993 685 524 391 191 148
49
SfoI GGC'GCC
Cuts at: 2756 3004 2756
Size: 248 5076
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5324
SmaI CCC'GGG
Cuts at: 2218 2346 2218
Size: 128 5196
SmlI C'TyrA_G
Cuts at: 130 1183 1387 2479 3394 3886 4148 130
Size: 1053 204 1092 915 492 262 1306
Fragments arranged by size:
1306 1092 1053 915 492 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5324
SphI G_CATG'C
Cuts at: 561 2377 2877 561
Size: 1816 500 3008
Fragments arranged by size:
3008 1816 500
StyI C'CwwG_G
Cuts at: 107 1541 2458 2812 107
Size: 1434 917 354 2619
Fragments arranged by size:
2619 1434 917 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1164 2115 2117 2907 3040 3499 3708 1164
Size: 951 2 790 133 459 209 2780
Fragments arranged by size:
2780 951 790 459 209 133 2
TatI w'GTAC_w
Cuts at: 1011 4839 1011
Size: 3828 1496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 871 903 4841 4873 871
Size: 32 3938 32 1322
Fragments arranged by size:
3938 1322 32 32
Tth111I GACn'n_nGTC
Cuts at: 3121 3121
Size: 5324
XbaI T'CTAG_A
Cuts at: 2355 2355
Size: 5324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 984 2819 984
Size: 1835 3489
XmaI C'CCGG_G
Cuts at: 2216 2344 2216
Size: 128 5196
XmnI GAAnn'nnTTC
Cuts at: 1778 2026 1778
Size: 248 5076
ZraI GAC'GTC
Cuts at: 1434 2190 1434
Size: 756 4568
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AlfI AloI AscI AvrII BbvCI BcgI BfrBI
BglI BmtI Bpu10I BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I CspCI EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI