Restriction map for
pF1KB9426
(Circular) (Six-base) MAPSORT of: pF1KB9426.seq Check: 6919 from: 1 to: 5162
pF1KB9426 5162 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1712 1823 2200 1712
Size: 111 377 4674
Fragments arranged by size:
4674 377 111
Acc65I G'GTAC_C
Cuts at: 2178 2178
Size: 5162
AclI AA'CG_TT
Cuts at: 5002 5002
Size: 5162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 913 1285 2987 3419 4166 4434 551
Size: 362 372 1702 432 747 268 1279
Fragments arranged by size:
1702 1279 747 432 372 362 268
AflIII A'CryG_T
Cuts at: 2514 3618 4595 2514
Size: 1104 977 3081
Fragments arranged by size:
3081 1104 977
AgeI A'CCGG_T
Cuts at: 3550 3550
Size: 5162
AlwNI CAG_nnn'CTG
Cuts at: 228 429 2467 4034 228
Size: 201 2038 1567 1356
Fragments arranged by size:
2038 1567 1356 201
ApaI G_GGCC'C
Cuts at: 1968 1968
Size: 5162
ApaLI G'TGCA_C
Cuts at: 723 3932 723
Size: 3209 1953
ApoI r'AATT_y
Cuts at: 2166 4515 4771 2166
Size: 2349 256 2557
Fragments arranged by size:
2557 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5162
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
AvaI C'yCGr_G
Cuts at: 352 478 2018 2182 352
Size: 126 1540 164 3332
Fragments arranged by size:
3332 1540 164 126
BamHI G'GATC_C
Cuts at: 1322 1856 2187 1322
Size: 534 331 4297
Fragments arranged by size:
4297 534 331
BanI G'GyrC_C
Cuts at: 118 445 2178 2592 2840 2875 118
Size: 327 1733 414 248 35 2405
Fragments arranged by size:
2405 1733 414 327 248 35
BanII G_rGCy'C
Cuts at: 1476 1968 2176 3206 1476
Size: 492 208 1030 3432
Fragments arranged by size:
3432 1030 492 208
BbeI G_GCGC'C
Cuts at: 449 2596 2844 449
Size: 2147 248 2767
Fragments arranged by size:
2767 2147 248
BbsI GAAGACnn'nnnn_
Cuts at: 298 357 298
Size: 59 5103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 714 748 714
Size: 34 5128
BciVI GTATCCnnnnn_n'
Cuts at: 3060 3821 5162 3060
Size: 761 1341 3060
Fragments arranged by size:
3060 1341 761
BclI T'GATC_A
Cuts at: 2682 2682
Size: 5162
BglII A'GATC_T
Cuts at: 1542 2677 4714 1542
Size: 1135 2037 1990
Fragments arranged by size:
2037 1990 1135
BlpI GC'TnA_GC
Cuts at: 2274 2274
Size: 5162
Bme1580I G_kGCm'C
Cuts at: 727 1968 2787 2880 3936 727
Size: 1241 819 93 1056 1953
Fragments arranged by size:
1953 1241 1056 819 93
BmgBI CAC'GTC
Cuts at: 1275 1275
Size: 5162
BmrI ACTGGGnnnn_n'
Cuts at: 1971 2374 2455 2790 4934 1971
Size: 403 81 335 2144 2199
Fragments arranged by size:
2199 2144 403 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3529 3529
Size: 5162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1658 1669 2126 526
Size: 1132 11 457 3562
Fragments arranged by size:
3562 1132 457 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1953 2338 3217 3709 4007 1953
Size: 385 879 492 298 3108
Fragments arranged by size:
3108 879 492 385 298
BsaI GGTCTCn'nnnn_
Cuts at: 542 1042 1235 4866 542
Size: 500 193 3631 838
Fragments arranged by size:
3631 838 500 193
BsaAI yAC'GTr
Cuts at: 308 638 2515 308
Size: 330 1877 2955
Fragments arranged by size:
2955 1877 330
BsaBI GATnn'nnATC
Cuts at: 6 835 2676 4713 6
Size: 829 1841 2037 455
Fragments arranged by size:
2037 1841 829 455
BsaHI Gr'CG_yC
Cuts at: 376 446 2593 2841 3543 5039 376
Size: 70 2147 248 702 1496 499
Fragments arranged by size:
2147 1496 702 499 248 70
BsaWI w'CCGG_w
Cuts at: 551 2351 2872 3550 3824 3971 4382 551
Size: 1800 521 678 274 147 411 1331
Fragments arranged by size:
1800 1331 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 331
Size: 5162
BseYI C'CCAG_C
Cuts at: 1967 2587 3345 3922 4418 1967
Size: 620 758 577 496 2711
Fragments arranged by size:
2711 758 620 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 393
Size: 5162
BsiEI CG_ry'CG
Cuts at: 104 443 1389 2704 2750 3958 104
Size: 339 946 1315 46 1208 1308
Fragments arranged by size:
1315 1308 1208 946 339 46
BsiHKAI G_wGCw'C
Cuts at: 727 1476 1925 2176 2954 3144 3936 727
Size: 749 449 251 778 190 792 1953
Fragments arranged by size:
1953 792 778 749 449 251 190
BsmI GAATG_Cn'
Cuts at: 1306 1306
Size: 5162
BsmBI CGTCTCn'nnnn_
Cuts at: 367 367
Size: 5162
Bsp1286I G_dGCh'C
Cuts at: 727 1476 1925 1968 2176 2787 2880 2954 3144
Size: 749 449 43 208 611 93 74 190
Cuts at: 3144 3206 3936 727
Size: 62 730 1953
Fragments arranged by size:
1953 749 730 611 449 208 190 93
74 62 43
BspEI T'CCGG_A
Cuts at: 4382 4382
Size: 5162
BspHI T'CATG_A
Cuts at: 1871 4338 1871
Size: 2467 2695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2212 2426 2728 3109 2212
Size: 214 302 381 4265
Fragments arranged by size:
4265 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3454 3508 4992 5157 50
Size: 3404 54 1484 165 55
Fragments arranged by size:
3404 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1632 1730 3074 1632
Size: 98 1344 3720
Fragments arranged by size:
3720 1344 98
BsrFI r'CCGG_y
Cuts at: 442 3160 3341 3550 442
Size: 2718 181 209 2054
Fragments arranged by size:
2718 2054 209 181
BssSI C'ACGA_G
Cuts at: 370 1469 1573 3433 3791 370
Size: 1099 104 1860 358 1741
Fragments arranged by size:
1860 1741 1099 358 104
BstAPI GCAn_nnn'nTGC
Cuts at: 2539 2539
Size: 5162
BstBI TT'CG_AA
Cuts at: 3523 3523
Size: 5162
BstXI CCAn_nnnn'nTGG
Cuts at: 616 1023 1896 616
Size: 407 873 3882
Fragments arranged by size:
3882 873 407
BstYI r'GATC_y
Cuts at: 1 474 554 1322 1542 1679 1856 2187 2654
Size: 473 80 768 220 137 177 331 467
Cuts at: 2654 2677 3012 3258 4270 4356 4368 4714 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 768 473 467 449 346 335 331
246 220 177 137 86 80 23 12
BtgI C'CryG_G
Cuts at: 1637 1637
Size: 5162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 3112 3293 3456 1963
Size: 1149 181 163 3669
Fragments arranged by size:
3669 1149 181 163
BtsI GCAGTG_nn'
Cuts at: 170 666 898 2405 170
Size: 496 232 1507 2927
Fragments arranged by size:
2927 1507 496 232
DraI TTT'AAA
Cuts at: 2161 2161
Size: 5162
DraIII CAC_nnn'GTG
Cuts at: 399 399
Size: 5162
DrdI GACnn_nn'nnGTC
Cuts at: 2868 3726 2868
Size: 858 4304
EaeI y'GGCC_r
Cuts at: 345 359 401 632 1640 2747 2921 3312 3339
Size: 14 42 231 1008 1107 174 391 27
Cuts at: 3339 4684 345
Size: 1345 823
Fragments arranged by size:
1345 1107 1008 823 391 231 174 42
27 14
EagI C'GGCC_G
Cuts at: 2747 2747
Size: 5162
EarI CTCTTCn'nnn_
Cuts at: 211 2054 3185 3395 211
Size: 1843 1131 210 1978
Fragments arranged by size:
1978 1843 1131 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3370 3680 3826 4972 3370
Size: 310 146 1146 3560
Fragments arranged by size:
3560 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1474 2174 1474
Size: 700 4462
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 551 913 1285 1658 1669 2126 2987 3419
Size: 25 362 372 373 11 457 861 432
Cuts at: 3419 4166 4434 526
Size: 747 268 1254
Fragments arranged by size:
1254 861 747 457 432 373 372 362
268 25 11
EcoNI CCTnn'n_nnAGG
Cuts at: 1795 1909 1795
Size: 114 5048
EcoO109I rG'GnC_Cy
Cuts at: 2301 2301
Size: 5162
EcoRI G'AATT_C
Cuts at: 2166 2166
Size: 5162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 5162
FspI TGC'GCA
Cuts at: 436 2943 436
Size: 2507 2655
HaeII r_GCGC'y
Cuts at: 449 582 2596 2844 3866 4845 449
Size: 133 2014 248 1022 979 766
Fragments arranged by size:
2014 1022 979 766 248 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 539 571 1145 1177 1314 1346 1371 1403 1994
Size: 32 574 32 137 32 25 32 591
Cuts at: 1994 2012 2026 2044 3004 3034 3036 3066 539
Size: 18 14 18 960 30 2 30 2635
Fragments arranged by size:
2635 960 591 574 137 32 32 32
32 30 30 25 18 18 14 2
HincII GTy'rAC
Cuts at: 2201 2201
Size: 5162
KasI G'GCGC_C
Cuts at: 445 2592 2840 445
Size: 2147 248 2767
Fragments arranged by size:
2767 2147 248
KpnI G_GTAC'C
Cuts at: 2182 2182
Size: 5162
MfeI C'AATT_G
Cuts at: 2078 2078
Size: 5162
MluI A'CGCG_T
Cuts at: 4595 4595
Size: 5162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1065 1084 1172 1547 3833 4017 1065
Size: 19 88 375 2286 184 2210
Fragments arranged by size:
2286 2210 375 184 88 19
MscI TGG'CCA
Cuts at: 403 634 2923 403
Size: 231 2289 2642
Fragments arranged by size:
2642 2289 231
MslI CAynn'nnrTG
Cuts at: 1183 1844 3151 3278 1183
Size: 661 1307 127 3067
Fragments arranged by size:
3067 1307 661 127
MspA1I CmG'CkG
Cuts at: 185 225 1971 2273 2587 2947 3960 4205 185
Size: 40 1746 302 314 360 1013 245 1142
Fragments arranged by size:
1746 1142 1013 360 314 302 245 40
NaeI GCC'GGC
Cuts at: 3343 3343
Size: 5162
NarI GG'CG_CC
Cuts at: 446 2593 2841 446
Size: 2147 248 2767
Fragments arranged by size:
2767 2147 248
NgoMIV G'CCGG_C
Cuts at: 3341 3341
Size: 5162
NspI r_CATG'y
Cuts at: 2215 2715 3622 2215
Size: 500 907 3755
Fragments arranged by size:
3755 907 500
PasI CC'CwG_GG
Cuts at: 381 381
Size: 5162
PciI A'CATG_T
Cuts at: 3618 3618
Size: 5162
PflMI CCAn_nnn'nTGG
Cuts at: 185 228 399 185
Size: 43 171 4948
Fragments arranged by size:
4948 171 43
PfoI T'CCnGG_A
Cuts at: 422 1685 422
Size: 1263 3899
PmeI GTTT'AAAC
Cuts at: 2161 2161
Size: 5162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 4301
Size: 32 5130
PspOMI G'GGCC_C
Cuts at: 1964 1964
Size: 5162
PspXI vC'TCGA_Gb
Cuts at: 352 352
Size: 5162
PstI C_TGCA'G
Cuts at: 176 419 673 1286 1491 1710 2209 2894 176
Size: 243 254 613 205 219 499 685 2444
Fragments arranged by size:
2444 685 613 499 254 243 219 205
PvuI CG_AT'CG
Cuts at: 104 1389 104
Size: 1285 3877
PvuII CAG'CTG
Cuts at: 225 1971 2587 2947 225
Size: 1746 616 360 2440
Fragments arranged by size:
2440 1746 616 360
RsrII CG'GwC_CG
Cuts at: 3357 3357
Size: 5162
SacI G_AGCT'C
Cuts at: 1476 2176 1476
Size: 700 4462
SalI G'TCGA_C
Cuts at: 2199 2199
Size: 5162
SapI GCTCTTCn'nnn_
Cuts at: 211 2054 3185 3395 211
Size: 1843 1131 210 1978
Fragments arranged by size:
1978 1843 1131 210
SbfI CC_TGCA'GG
Cuts at: 2209 2209
Size: 5162
ScaI AGT'ACT
Cuts at: 4679 4679
Size: 5162
SexAI A'CCwGG_T
Cuts at: 257 257
Size: 5162
SfcI C'TryA_G
Cuts at: 33 172 415 669 1282 1487 1536 1706 2205
Size: 139 243 254 613 205 49 170 499
Cuts at: 2205 2890 3883 4074 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 613 499 254 243 205
191 170 139 49
SfoI GGC'GCC
Cuts at: 447 2594 2842 447
Size: 2147 248 2767
Fragments arranged by size:
2767 2147 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5162
SmaI CCC'GGG
Cuts at: 2184 2184
Size: 5162
SmlI C'TyrA_G
Cuts at: 352 478 1932 2317 3232 3724 3986 352
Size: 126 1454 385 915 492 262 1528
Fragments arranged by size:
1528 1454 915 492 385 262 126
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5162
SphI G_CATG'C
Cuts at: 2215 2715 2215
Size: 500 4662
SspI AAT'ATT
Cuts at: 789 1053 789
Size: 264 4898
StyI C'CwwG_G
Cuts at: 2296 2650 2296
Size: 354 4808
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 459 1327 2745 2878 3337 3546 459
Size: 868 1418 133 459 209 2075
Fragments arranged by size:
2075 1418 868 459 209 133
TatI w'GTAC_w
Cuts at: 772 4677 772
Size: 3905 1257
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4679 4711 4679
Size: 32 5130
Tth111I GACn'n_nGTC
Cuts at: 1558 2959 1558
Size: 1401 3761
XbaI T'CTAG_A
Cuts at: 2193 2193
Size: 5162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 613 1893 2657 613
Size: 1280 764 3118
Fragments arranged by size:
3118 1280 764
XhoI C'TCGA_G
Cuts at: 352 478 352
Size: 126 5036
XmaI C'CCGG_G
Cuts at: 2182 2182
Size: 5162
XmnI GAAnn'nnTTC
Cuts at: 1053 2127 1053
Size: 1074 4088
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I
BsaXI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PpuMI PshAI
PsiI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI ZraI