Restriction map for
pF1KB9429
(Circular) (Six-base) MAPSORT of: pF1KB9429.seq Check: 6928 from: 1 to: 4793
pF1KB9429 4793 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1190 1831 1190
Size: 641 4152
Acc65I G'GTAC_C
Cuts at: 1809 1809
Size: 4793
AclI AA'CG_TT
Cuts at: 4633 4633
Size: 4793
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 537 967 1192 1477 2618 3050 3797 4065
Size: 337 430 225 285 1141 432 747 268
Cuts at: 4065 200
Size: 928
Fragments arranged by size:
1141 928 747 432 430 337 285 268
225
AfeI AGC'GCT
Cuts at: 1171 1348 1171
Size: 177 4616
AflIII A'CryG_T
Cuts at: 2145 3249 4226 2145
Size: 1104 977 2712
Fragments arranged by size:
2712 1104 977
AgeI A'CCGG_T
Cuts at: 3181 3181
Size: 4793
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1598 1598
Size: 4793
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1409 1441 1409
Size: 32 4761
AlwNI CAG_nnn'CTG
Cuts at: 126 375 2098 3665 126
Size: 249 1723 1567 1254
Fragments arranged by size:
1723 1567 1254 249
ApaLI G'TGCA_C
Cuts at: 3563 3563
Size: 4793
ApoI r'AATT_y
Cuts at: 583 916 1797 4146 4402 583
Size: 333 881 2349 256 974
Fragments arranged by size:
2349 974 881 333 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4793
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4793
AvaI C'yCGr_G
Cuts at: 190 1813 190
Size: 1623 3170
BamHI G'GATC_C
Cuts at: 1545 1818 1545
Size: 273 4520
BanI G'GyrC_C
Cuts at: 1264 1689 1809 2223 2471 2506 1264
Size: 425 120 414 248 35 3551
Fragments arranged by size:
3551 425 414 248 120 35
BanII G_rGCy'C
Cuts at: 1807 2837 1807
Size: 1030 3763
BbeI G_GCGC'C
Cuts at: 2227 2475 2227
Size: 248 4545
BbsI GAAGACnn'nnnn_
Cuts at: 362 635 674 698 973 1537 362
Size: 273 39 24 275 564 3618
Fragments arranged by size:
3618 564 275 273 39 24
BbvCI CC'TCA_GC
Cuts at: 482 482
Size: 4793
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 457 491 457
Size: 34 4759
BciVI GTATCCnnnnn_n'
Cuts at: 2691 3452 4793 2691
Size: 761 1341 2691
Fragments arranged by size:
2691 1341 761
BclI T'GATC_A
Cuts at: 501 2313 501
Size: 1812 2981
BglII A'GATC_T
Cuts at: 2308 4345 2308
Size: 2037 2756
BlpI GC'TnA_GC
Cuts at: 1388 1905 1388
Size: 517 4276
Bme1580I G_kGCm'C
Cuts at: 930 2418 2511 3567 930
Size: 1488 93 1056 2156
Fragments arranged by size:
2156 1488 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 451 1258 2005 2086 2421 4565 451
Size: 807 747 81 335 2144 679
Fragments arranged by size:
2144 807 747 679 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3160 3160
Size: 4793
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 469 722 1156 440
Size: 29 253 434 4077
Fragments arranged by size:
4077 434 253 29
Bpu10I CC'TnA_GC
Cuts at: 482 1376 1666 482
Size: 894 290 3609
Fragments arranged by size:
3609 894 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 1969 2848 3340 3638 1418
Size: 551 879 492 298 2573
Fragments arranged by size:
2573 879 551 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4497 4497
Size: 4793
BsaAI yAC'GTr
Cuts at: 2146 2146
Size: 4793
BsaBI GATnn'nnATC
Cuts at: 6 2307 4344 6
Size: 2301 2037 455
Fragments arranged by size:
2301 2037 455
BsaHI Gr'CG_yC
Cuts at: 2224 2472 3174 4670 2224
Size: 248 702 1496 2347
Fragments arranged by size:
2347 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1419 1982 2503 3181 3455 3602 4013 1419
Size: 563 521 678 274 147 411 2199
Fragments arranged by size:
2199 678 563 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 634 637 1072 634
Size: 3 435 4355
Fragments arranged by size:
4355 435 3
BseYI C'CCAG_C
Cuts at: 1153 2218 2976 3553 4049 1153
Size: 1065 758 577 496 1897
Fragments arranged by size:
1897 1065 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2335 2381 3589 104
Size: 2231 46 1208 1308
Fragments arranged by size:
2231 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1807 2585 2775 3567 1807
Size: 778 190 792 3033
Fragments arranged by size:
3033 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 930 1807 2418 2511 2585 2775 2837 3567 930
Size: 877 611 93 74 190 62 730 2156
Fragments arranged by size:
2156 877 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4013 4013
Size: 4793
BspHI T'CATG_A
Cuts at: 3969 3969
Size: 4793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1843 2057 2359 2740 1843
Size: 214 302 381 3896
Fragments arranged by size:
3896 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1742 3085 3139 4623 4788 50
Size: 1692 1343 54 1484 165 55
Fragments arranged by size:
1692 1484 1343 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2705 2705
Size: 4793
BsrFI r'CCGG_y
Cuts at: 2791 2972 3181 2791
Size: 181 209 4403
Fragments arranged by size:
4403 209 181
BssSI C'ACGA_G
Cuts at: 3064 3422 3064
Size: 358 4435
BstAPI GCAn_nnn'nTGC
Cuts at: 375 2170 375
Size: 1795 2998
BstBI TT'CG_AA
Cuts at: 1329 3154 1329
Size: 1825 2968
BstXI CCAn_nnnn'nTGG
Cuts at: 597 1161 1542 597
Size: 564 381 3848
Fragments arranged by size:
3848 564 381
BstYI r'GATC_y
Cuts at: 1 1424 1545 1818 2285 2308 2643 2889 3901
Size: 1423 121 273 467 23 335 246 1012
Cuts at: 3901 3987 3999 4345 1
Size: 86 12 346 449
Fragments arranged by size:
1423 1012 467 449 346 335 273 246
121 86 23 12
BtgI C'CryG_G
Cuts at: 107 1565 1779 107
Size: 1458 214 3121
Fragments arranged by size:
3121 1458 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2743 2924 3087 2743
Size: 181 163 4449
Fragments arranged by size:
4449 181 163
BtsI GCAGTG_nn'
Cuts at: 200 621 1582 2036 200
Size: 421 961 454 2957
Fragments arranged by size:
2957 961 454 421
DraI TTT'AAA
Cuts at: 1792 1792
Size: 4793
DrdI GACnn_nn'nnGTC
Cuts at: 1186 2499 3357 1186
Size: 1313 858 2622
Fragments arranged by size:
2622 1313 858
EaeI y'GGCC_r
Cuts at: 1562 1752 2378 2552 2943 2970 4315 1562
Size: 190 626 174 391 27 1345 2040
Fragments arranged by size:
2040 1345 626 391 190 174 27
EagI C'GGCC_G
Cuts at: 2378 2378
Size: 4793
EarI CTCTTCn'nnn_
Cuts at: 514 629 774 983 1085 2816 3026 514
Size: 115 145 209 102 1731 210 2281
Fragments arranged by size:
2281 1731 210 209 145 115 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 187 3001 3311 3457 4603 187
Size: 2814 310 146 1146 377
Fragments arranged by size:
2814 1146 377 310 146
EcoICRI GAG'CTC
Cuts at: 1805 1805
Size: 4793
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 440 469 537 722 967 1156 1192 1477
Size: 240 29 68 185 245 189 36 285
Cuts at: 1477 2618 3050 3797 4065 200
Size: 1141 432 747 268 928
Fragments arranged by size:
1141 928 747 432 285 268 245 240
189 185 68 36 29
EcoO109I rG'GnC_Cy
Cuts at: 1116 1932 1116
Size: 816 3977
EcoRI G'AATT_C
Cuts at: 1797 1797
Size: 4793
EcoRV GAT'ATC
Cuts at: 1021 1021
Size: 4793
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1012 2812 1012
Size: 1800 2993
FspI TGC'GCA
Cuts at: 2574 2574
Size: 4793
HaeII r_GCGC'y
Cuts at: 133 1173 1350 2227 2475 3497 4476 133
Size: 1040 177 877 248 1022 979 450
Fragments arranged by size:
1040 1022 979 877 450 248 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 400 417 432 1416 1448 2635 2665 2667
Size: 15 17 15 984 32 1187 30 2
Cuts at: 2667 2697 385
Size: 30 2481
Fragments arranged by size:
2481 1187 984 32 30 30 17 15
15 2
HincII GTy'rAC
Cuts at: 854 1832 854
Size: 978 3815
KasI G'GCGC_C
Cuts at: 2223 2471 2223
Size: 248 4545
KpnI G_GTAC'C
Cuts at: 1813 1813
Size: 4793
MluI A'CGCG_T
Cuts at: 4226 4226
Size: 4793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3464 3648 3464
Size: 184 4609
MscI TGG'CCA
Cuts at: 1564 1754 2554 1564
Size: 190 800 3803
Fragments arranged by size:
3803 800 190
MslI CAynn'nnrTG
Cuts at: 1784 2782 2909 1784
Size: 998 127 3668
Fragments arranged by size:
3668 998 127
MspA1I CmG'CkG
Cuts at: 1904 2218 2578 3591 3836 1904
Size: 314 360 1013 245 2861
Fragments arranged by size:
2861 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2974 2974
Size: 4793
NarI GG'CG_CC
Cuts at: 2224 2472 2224
Size: 248 4545
NcoI C'CATG_G
Cuts at: 107 1565 107
Size: 1458 3335
NgoMIV G'CCGG_C
Cuts at: 2972 2972
Size: 4793
NspI r_CATG'y
Cuts at: 1846 2346 3253 1846
Size: 500 907 3386
Fragments arranged by size:
3386 907 500
PciI A'CATG_T
Cuts at: 3249 3249
Size: 4793
PflMI CCAn_nnn'nTGG
Cuts at: 819 819
Size: 4793
PmeI GTTT'AAAC
Cuts at: 1792 1792
Size: 4793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 3932
Size: 32 4761
PpuMI rG'GwC_Cy
Cuts at: 1116 1116
Size: 4793
PstI C_TGCA'G
Cuts at: 1840 2525 1840
Size: 685 4108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4793
PvuII CAG'CTG
Cuts at: 2218 2578 2218
Size: 360 4433
RsrII CG'GwC_CG
Cuts at: 2988 2988
Size: 4793
SacI G_AGCT'C
Cuts at: 1807 1807
Size: 4793
SalI G'TCGA_C
Cuts at: 1830 1830
Size: 4793
SanDI GG'GwC_CC
Cuts at: 1116 1116
Size: 4793
SapI GCTCTTCn'nnn_
Cuts at: 514 774 2816 3026 514
Size: 260 2042 210 2281
Fragments arranged by size:
2281 2042 260 210
SbfI CC_TGCA'GG
Cuts at: 1840 1840
Size: 4793
ScaI AGT'ACT
Cuts at: 4310 4310
Size: 4793
SfcI C'TryA_G
Cuts at: 33 1191 1836 2521 3514 3705 33
Size: 1158 645 685 993 191 1121
Fragments arranged by size:
1158 1121 993 685 645 191
SfoI GGC'GCC
Cuts at: 2225 2473 2225
Size: 248 4545
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4793
SmaI CCC'GGG
Cuts at: 1815 1815
Size: 4793
SmlI C'TyrA_G
Cuts at: 1433 1948 2863 3355 3617 1433
Size: 515 915 492 262 2609
Fragments arranged by size:
2609 915 515 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4793
SphI G_CATG'C
Cuts at: 1846 2346 1846
Size: 500 4293
StyI C'CwwG_G
Cuts at: 107 590 606 675 1541 1565 1927 2281 107
Size: 483 16 69 866 24 362 354 2619
Fragments arranged by size:
2619 866 483 362 354 69 24 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 463 2376 2509 2968 3177 463
Size: 1913 133 459 209 2079
Fragments arranged by size:
2079 1913 459 209 133
TatI w'GTAC_w
Cuts at: 4308 4308
Size: 4793
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 478 510 770 802 4310 4342 478
Size: 32 260 32 3508 32 929
Fragments arranged by size:
3508 929 260 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 443 2590 443
Size: 2147 2646
XbaI T'CTAG_A
Cuts at: 337 1824 337
Size: 1487 3306
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1557 2288 1557
Size: 731 4062
XmaI C'CCGG_G
Cuts at: 1813 1813
Size: 4793
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI