Restriction map for
pF1KB9430
(Circular) (Six-base) MAPSORT of: pF1KB9430.seq Check: 3338 from: 1 to: 4412
pF1KB9430 4412 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 995 1450 995
Size: 455 3957
Acc65I G'GTAC_C
Cuts at: 1428 1428
Size: 4412
AclI AA'CG_TT
Cuts at: 4252 4252
Size: 4412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1233 2237 2669 3416 3684 1233
Size: 1004 432 747 268 1961
Fragments arranged by size:
1961 1004 747 432 268
AfeI AGC'GCT
Cuts at: 146 947 146
Size: 801 3611
AflIII A'CryG_T
Cuts at: 1115 1764 2868 3845 1115
Size: 649 1104 977 1682
Fragments arranged by size:
1682 1104 977 649
AgeI A'CCGG_T
Cuts at: 2800 2800
Size: 4412
AlwNI CAG_nnn'CTG
Cuts at: 799 1717 3284 799
Size: 918 1567 1927
Fragments arranged by size:
1927 1567 918
ApaLI G'TGCA_C
Cuts at: 3182 3182
Size: 4412
ApoI r'AATT_y
Cuts at: 1416 3765 4021 1416
Size: 2349 256 1807
Fragments arranged by size:
2349 1807 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4412
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
AvaI C'yCGr_G
Cuts at: 171 184 252 270 957 1158 1339 1359 1372
Size: 13 68 18 687 201 181 20 13
Cuts at: 1372 1432 171
Size: 60 3151
Fragments arranged by size:
3151 687 201 181 68 60 20 18
13 13
BamHI G'GATC_C
Cuts at: 1437 1437
Size: 4412
BanI G'GyrC_C
Cuts at: 175 670 859 1428 1842 2090 2125 175
Size: 495 189 569 414 248 35 2462
Fragments arranged by size:
2462 569 495 414 248 189 35
BanII G_rGCy'C
Cuts at: 166 1165 1298 1426 2456 166
Size: 999 133 128 1030 2122
Fragments arranged by size:
2122 1030 999 133 128
BbeI G_GCGC'C
Cuts at: 179 674 863 1846 2094 179
Size: 495 189 983 248 2497
Fragments arranged by size:
2497 983 495 248 189
BbsI GAAGACnn'nnnn_
Cuts at: 1196 1196
Size: 4412
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3071 4412 2310
Size: 761 1341 2310
Fragments arranged by size:
2310 1341 761
BclI T'GATC_A
Cuts at: 1932 1932
Size: 4412
BglI GCCn_nnn'nGGC
Cuts at: 608 829 925 608
Size: 221 96 4095
Fragments arranged by size:
4095 221 96
BglII A'GATC_T
Cuts at: 371 1927 3964 371
Size: 1556 2037 819
Fragments arranged by size:
2037 1556 819
BlpI GC'TnA_GC
Cuts at: 1524 1524
Size: 4412
Bme1580I G_kGCm'C
Cuts at: 281 1018 2037 2130 3186 281
Size: 737 1019 93 1056 1507
Fragments arranged by size:
1507 1056 1019 737 93
BmgBI CAC'GTC
Cuts at: 1019 1019
Size: 4412
BmrI ACTGGGnnnn_n'
Cuts at: 1624 1705 2040 4184 1624
Size: 81 335 2144 1852
Fragments arranged by size:
2144 1852 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1317 1374 1387 2779 1317
Size: 57 13 1392 2950
Fragments arranged by size:
2950 1392 57 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 513
Size: 4412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1588 2467 2959 3257 442
Size: 1146 879 492 298 1597
Fragments arranged by size:
1597 1146 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4116 4116
Size: 4412
BsaAI yAC'GTr
Cuts at: 1765 1765
Size: 4412
BsaBI GATnn'nnATC
Cuts at: 6 1926 3963 6
Size: 1920 2037 455
Fragments arranged by size:
2037 1920 455
BsaHI Gr'CG_yC
Cuts at: 176 671 860 1843 2091 2793 4289 176
Size: 495 189 983 248 702 1496 299
Fragments arranged by size:
1496 983 702 495 299 248 189
BsaWI w'CCGG_w
Cuts at: 1601 2122 2800 3074 3221 3632 1601
Size: 521 678 274 147 411 2381
Fragments arranged by size:
2381 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 511 517 541 547 563 593 1105 1135 511
Size: 6 24 6 16 30 512 30 3788
Fragments arranged by size:
3788 512 30 30 24 16 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 192 525 540 659 912 1113 1131 1134 1143
Size: 333 15 119 253 201 18 3 9
Cuts at: 1143 1146 1155 192
Size: 3 9 3449
Fragments arranged by size:
3449 333 253 201 119 18 15 9
9 3 3
BseYI C'CCAG_C
Cuts at: 189 384 901 1213 1837 2595 3172 3668 189
Size: 195 517 312 624 758 577 496 933
Fragments arranged by size:
933 758 624 577 517 496 312 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 1317 133
Size: 1184 3228
BsiEI CG_ry'CG
Cuts at: 104 542 1041 1272 1954 2000 3208 104
Size: 438 499 231 682 46 1208 1308
Fragments arranged by size:
1308 1208 682 499 438 231 46
BsiHKAI G_wGCw'C
Cuts at: 166 1426 2204 2394 3186 166
Size: 1260 778 190 792 1392
Fragments arranged by size:
1392 1260 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 166 281 1018 1165 1298 1426 2037 2130 2204
Size: 115 737 147 133 128 611 93 74
Cuts at: 2204 2394 2456 3186 166
Size: 190 62 730 1392
Fragments arranged by size:
1392 737 730 611 190 147 133 128
115 93 74 62
BspEI T'CCGG_A
Cuts at: 3632 3632
Size: 4412
BspHI T'CATG_A
Cuts at: 3588 3588
Size: 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1205 1462 1676 1978 2359 1205
Size: 257 214 302 381 3258
Fragments arranged by size:
3258 381 302 257 214
BsrBI CCG'CTC
Cuts at: 50 327 447 1043 2704 2758 4242 4407 50
Size: 277 120 596 1661 54 1484 165 55
Fragments arranged by size:
1661 1484 596 277 165 120 55 54
BsrDI GCAATG_nn'
Cuts at: 2324 2324
Size: 4412
BsrFI r'CCGG_y
Cuts at: 154 178 491 684 1036 2410 2591 2800 154
Size: 24 313 193 352 1374 181 209 1766
Fragments arranged by size:
1766 1374 352 313 209 193 181 24
BsrGI T'GTAC_A
Cuts at: 884 884
Size: 4412
BssHII G'CGCG_C
Cuts at: 1077 1103 1077
Size: 26 4386
BssSI C'ACGA_G
Cuts at: 2683 3041 2683
Size: 358 4054
BstAPI GCAn_nnn'nTGC
Cuts at: 799 1789 799
Size: 990 3422
BstBI TT'CG_AA
Cuts at: 1187 2773 1187
Size: 1586 2826
BstXI CCAn_nnnn'nTGG
Cuts at: 384 384
Size: 4412
BstYI r'GATC_y
Cuts at: 1 371 422 1437 1904 1927 2262 2508 3520
Size: 370 51 1015 467 23 335 246 1012
Cuts at: 3520 3606 3618 3964 1
Size: 86 12 346 449
Fragments arranged by size:
1015 1012 467 449 370 346 335 246
86 51 23 12
BtgI C'CryG_G
Cuts at: 107 230 603 1059 107
Size: 123 373 456 3460
Fragments arranged by size:
3460 456 373 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2362 2543 2706 2362
Size: 181 163 4068
Fragments arranged by size:
4068 181 163
BtsI GCAGTG_nn'
Cuts at: 594 1655 594
Size: 1061 3351
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 564 599 564
Size: 35 4377
DraI TTT'AAA
Cuts at: 1411 1411
Size: 4412
DrdI GACnn_nn'nnGTC
Cuts at: 2118 2976 2118
Size: 858 3554
EaeI y'GGCC_r
Cuts at: 152 539 600 1038 1997 2171 2562 2589 3934
Size: 387 61 438 959 174 391 27 1345
Cuts at: 3934 152
Size: 630
Fragments arranged by size:
1345 959 630 438 391 387 174 61
27
EagI C'GGCC_G
Cuts at: 539 1038 1997 539
Size: 499 959 2954
Fragments arranged by size:
2954 959 499
EarI CTCTTCn'nnn_
Cuts at: 1152 2435 2645 1152
Size: 1283 210 2919
Fragments arranged by size:
2919 1283 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 457 906 988 2620 2930 3076 4222 457
Size: 449 82 1632 310 146 1146 647
Fragments arranged by size:
1632 1146 647 449 310 146 82
EcoICRI GAG'CTC
Cuts at: 164 1424 164
Size: 1260 3152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 1233 2237 2669 3416 3684 513
Size: 720 1004 432 747 268 1241
Fragments arranged by size:
1241 1004 747 720 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1275 1551 1275
Size: 276 4136
EcoRI G'AATT_C
Cuts at: 1416 1416
Size: 4412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4412
FspI TGC'GCA
Cuts at: 2193 2193
Size: 4412
HaeII r_GCGC'y
Cuts at: 148 179 674 863 908 949 1267 1846 2094
Size: 31 495 189 45 41 318 579 248
Cuts at: 2094 3116 4095 148
Size: 1022 979 465
Fragments arranged by size:
1022 979 579 495 465 318 248 189
45 41 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 563 595 862 894 1219 1251 2254 2284 2286
Size: 32 267 32 325 32 1003 30 2
Cuts at: 2286 2316 563
Size: 30 2659
Fragments arranged by size:
2659 1003 325 267 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1451 1451
Size: 4412
KasI G'GCGC_C
Cuts at: 175 670 859 1842 2090 175
Size: 495 189 983 248 2497
Fragments arranged by size:
2497 983 495 248 189
KpnI G_GTAC'C
Cuts at: 1432 1432
Size: 4412
MluI A'CGCG_T
Cuts at: 1115 3845 1115
Size: 2730 1682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1199 1412 3083 3267 1199
Size: 213 1671 184 2344
Fragments arranged by size:
2344 1671 213 184
MscI TGG'CCA
Cuts at: 2173 2173
Size: 4412
MslI CAynn'nnrTG
Cuts at: 2401 2528 2401
Size: 127 4285
MspA1I CmG'CkG
Cuts at: 796 1061 1523 1837 2197 3210 3455 796
Size: 265 462 314 360 1013 245 1753
Fragments arranged by size:
1753 1013 462 360 314 265 245
NaeI GCC'GGC
Cuts at: 156 180 1038 2593 156
Size: 24 858 1555 1975
Fragments arranged by size:
1975 1555 858 24
NarI GG'CG_CC
Cuts at: 176 671 860 1843 2091 176
Size: 495 189 983 248 2497
Fragments arranged by size:
2497 983 495 248 189
NcoI C'CATG_G
Cuts at: 107 603 107
Size: 496 3916
NgoMIV G'CCGG_C
Cuts at: 154 178 1036 2591 154
Size: 24 858 1555 1975
Fragments arranged by size:
1975 1555 858 24
NspI r_CATG'y
Cuts at: 360 1236 1465 1965 2872 360
Size: 876 229 500 907 1900
Fragments arranged by size:
1900 907 876 500 229
PasI CC'CwG_GG
Cuts at: 855 1239 855
Size: 384 4028
PciI A'CATG_T
Cuts at: 2868 2868
Size: 4412
PfoI T'CCnGG_A
Cuts at: 1322 1322
Size: 4412
PmeI GTTT'AAAC
Cuts at: 1411 1411
Size: 4412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1201 1233 3551 3583 1201
Size: 32 2318 32 2030
Fragments arranged by size:
2318 2030 32 32
PpuMI rG'GwC_Cy
Cuts at: 1275 1275
Size: 4412
PspXI vC'TCGA_Gb
Cuts at: 184 1372 184
Size: 1188 3224
PstI C_TGCA'G
Cuts at: 1459 2144 1459
Size: 685 3727
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4412
PvuII CAG'CTG
Cuts at: 1837 2197 1837
Size: 360 4052
RsrII CG'GwC_CG
Cuts at: 2607 2607
Size: 4412
SacI G_AGCT'C
Cuts at: 166 1426 166
Size: 1260 3152
SacII CC_GC'GG
Cuts at: 1062 1062
Size: 4412
SalI G'TCGA_C
Cuts at: 1449 1449
Size: 4412
SapI GCTCTTCn'nnn_
Cuts at: 2435 2645 2435
Size: 210 4202
SbfI CC_TGCA'GG
Cuts at: 1459 1459
Size: 4412
ScaI AGT'ACT
Cuts at: 3929 3929
Size: 4412
SexAI A'CCwGG_T
Cuts at: 1391 1391
Size: 4412
SfcI C'TryA_G
Cuts at: 33 1455 2140 3133 3324 33
Size: 1422 685 993 191 1121
Fragments arranged by size:
1422 1121 993 685 191
SfoI GGC'GCC
Cuts at: 177 672 861 1844 2092 177
Size: 495 189 983 248 2497
Fragments arranged by size:
2497 983 495 248 189
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
SmaI CCC'GGG
Cuts at: 959 1434 959
Size: 475 3937
SmlI C'TyrA_G
Cuts at: 184 457 1372 1567 2482 2974 3236 184
Size: 273 915 195 915 492 262 1360
Fragments arranged by size:
1360 915 915 492 273 262 195
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4412
SphI G_CATG'C
Cuts at: 1465 1965 1465
Size: 500 3912
StyI C'CwwG_G
Cuts at: 107 603 1546 1900 107
Size: 496 943 354 2619
Fragments arranged by size:
2619 943 496 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1995 2128 2587 2796 1995
Size: 133 459 209 3611
Fragments arranged by size:
3611 459 209 133
TatI w'GTAC_w
Cuts at: 884 3927 884
Size: 3043 1369
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1105 1137 3929 3961 1105
Size: 32 2792 32 1556
Fragments arranged by size:
2792 1556 32 32
Tth111I GACn'n_nGTC
Cuts at: 577 2209 577
Size: 1632 2780
XbaI T'CTAG_A
Cuts at: 1443 1443
Size: 4412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 762 1907 762
Size: 1145 3267
XhoI C'TCGA_G
Cuts at: 184 1372 184
Size: 1188 3224
XmaI C'CCGG_G
Cuts at: 957 1432 957
Size: 475 3937
XmnI GAAnn'nnTTC
Cuts at: 301 301
Size: 4412
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsiWI BsmI BsmBI BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspOMI PsrI SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI