(Circular) (Six-base) MAPSORT of: pF1KB9431.seq Check: 691 from: 1 to: 4685 pF1KB9431 4685 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 229 460 229 Size: 231 4454 AccI GT'mk_AC Cuts at: 1367 1723 1367 Size: 356 4329 Acc65I G'GTAC_C Cuts at: 1701 1701 Size: 4685 AclI AA'CG_TT Cuts at: 4525 4525 Size: 4685 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 547 1103 1267 1667 2510 2942 3689 3957 547 Size: 556 164 400 843 432 747 268 1275 Fragments arranged by size: 1275 843 747 556 432 400 268 164 AflIII A'CryG_T Cuts at: 2037 3141 4118 2037 Size: 1104 977 2604 Fragments arranged by size: 2604 1104 977 AgeI A'CCGG_T Cuts at: 947 3073 947 Size: 2126 2559 AleI CACnn'nnGTG Cuts at: 1534 1534 Size: 4685 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 731 731 Size: 4685 AlwNI CAG_nnn'CTG Cuts at: 696 744 1592 1990 3557 696 Size: 48 848 398 1567 1824 Fragments arranged by size: 1824 1567 848 398 48 ApaI G_GGCC'C Cuts at: 1519 1519 Size: 4685 ApaLI G'TGCA_C Cuts at: 3455 3455 Size: 4685 ApoI r'AATT_y Cuts at: 1689 4038 4294 1689 Size: 2349 256 2080 Fragments arranged by size: 2349 2080 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4685 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4685 AvaI C'yCGr_G Cuts at: 167 1411 1705 167 Size: 1244 294 3147 Fragments arranged by size: 3147 1244 294 BamHI G'GATC_C Cuts at: 1710 1710 Size: 4685 BanI G'GyrC_C Cuts at: 1435 1701 2115 2363 2398 1435 Size: 266 414 248 35 3722 Fragments arranged by size: 3722 414 266 248 35 BanII G_rGCy'C Cuts at: 1007 1349 1420 1447 1519 1586 1679 1699 2729 Size: 342 71 27 72 67 93 20 1030 Cuts at: 2729 1007 Size: 2963 Fragments arranged by size: 2963 1030 342 93 72 71 67 27 20 BbeI G_GCGC'C Cuts at: 2119 2367 2119 Size: 248 4437 BbsI GAAGACnn'nnnn_ Cuts at: 623 1160 1667 623 Size: 537 507 3641 Fragments arranged by size: 3641 537 507 BbvCI CC'TCA_GC Cuts at: 1148 1148 Size: 4685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 1206 1240 90 Size: 34 1082 34 3535 Fragments arranged by size: 3535 1082 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2583 3344 4685 2583 Size: 761 1341 2583 Fragments arranged by size: 2583 1341 761 BclI T'GATC_A Cuts at: 252 2205 252 Size: 1953 2732 BglI GCCn_nnn'nGGC Cuts at: 1210 1210 Size: 4685 BglII A'GATC_T Cuts at: 2200 4237 2200 Size: 2037 2648 BlpI GC'TnA_GC Cuts at: 1797 1797 Size: 4685 Bme1580I G_kGCm'C Cuts at: 1031 1519 2310 2403 3459 1031 Size: 488 791 93 1056 2257 Fragments arranged by size: 2257 1056 791 488 93 BmrI ACTGGGnnnn_n' Cuts at: 1580 1897 1978 2313 4457 1580 Size: 317 81 335 2144 1808 Fragments arranged by size: 2144 1808 335 317 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3052 3052 Size: 4685 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1297 1297 Size: 4685 Bpu10I CC'TnA_GC Cuts at: 866 1148 866 Size: 282 4403 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1861 2740 3232 3530 1861 Size: 879 492 298 3016 Fragments arranged by size: 3016 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 798 1552 4389 798 Size: 754 2837 1094 Fragments arranged by size: 2837 1094 754 BsaAI yAC'GTr Cuts at: 2038 2038 Size: 4685 BsaBI GATnn'nnATC Cuts at: 6 2199 4236 6 Size: 2193 2037 455 Fragments arranged by size: 2193 2037 455 BsaHI Gr'CG_yC Cuts at: 173 434 899 1618 2116 2364 3066 4562 173 Size: 261 465 719 498 248 702 1496 296 Fragments arranged by size: 1496 719 702 498 465 296 261 248 BsaWI w'CCGG_w Cuts at: 947 1874 2395 3073 3347 3494 3905 947 Size: 927 521 678 274 147 411 1727 Fragments arranged by size: 1727 927 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 466 496 466 Size: 30 4655 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 580 865 1027 1060 1063 1066 1069 1072 1075 Size: 285 162 33 3 3 3 3 3 Cuts at: 1075 1078 1081 1105 1108 1111 1114 1243 1273 Size: 3 3 24 3 3 3 129 30 Cuts at: 1273 580 Size: 3992 Fragments arranged by size: 3992 285 162 129 33 30 24 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 143 598 2110 2868 3445 3941 143 Size: 455 1512 758 577 496 887 Fragments arranged by size: 1512 887 758 577 496 455 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 321 1135 321 Size: 814 3871 BsiEI CG_ry'CG Cuts at: 104 918 1243 2227 2273 3481 104 Size: 814 325 984 46 1208 1308 Fragments arranged by size: 1308 1208 984 814 325 46 BsiHKAI G_wGCw'C Cuts at: 1349 1679 1699 2477 2667 3459 1349 Size: 330 20 778 190 792 2575 Fragments arranged by size: 2575 792 778 330 190 20 BsmBI CGTCTCn'nnnn_ Cuts at: 180 180 Size: 4685 Bsp1286I G_dGCh'C Cuts at: 1007 1031 1349 1420 1447 1519 1586 1679 1699 Size: 24 318 71 27 72 67 93 20 Cuts at: 1699 2310 2403 2477 2667 2729 3459 1007 Size: 611 93 74 190 62 730 2233 Fragments arranged by size: 2233 730 611 318 190 93 93 74 72 71 67 62 27 24 20 BspEI T'CCGG_A Cuts at: 3905 3905 Size: 4685 BspHI T'CATG_A Cuts at: 3861 3861 Size: 4685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 229 460 1735 1949 2251 2632 229 Size: 231 1275 214 302 381 2282 Fragments arranged by size: 2282 1275 381 302 231 214 BsrBI CCG'CTC Cuts at: 50 1239 1526 2977 3031 4515 4680 50 Size: 1189 287 1451 54 1484 165 55 Fragments arranged by size: 1484 1451 1189 287 165 55 54 BsrDI GCAATG_nn' Cuts at: 2597 2597 Size: 4685 BsrFI r'CCGG_y Cuts at: 947 1134 2683 2864 3073 947 Size: 187 1549 181 209 2559 Fragments arranged by size: 2559 1549 209 187 181 BsrGI T'GTAC_A Cuts at: 1328 1328 Size: 4685 BssSI C'ACGA_G Cuts at: 2956 3314 2956 Size: 358 4327 BstAPI GCAn_nnn'nTGC Cuts at: 985 1505 2062 985 Size: 520 557 3608 Fragments arranged by size: 3608 557 520 BstBI TT'CG_AA Cuts at: 3046 3046 Size: 4685 BstEII G'GTnAC_C Cuts at: 504 1566 504 Size: 1062 3623 BstYI r'GATC_y Cuts at: 1 533 1710 2177 2200 2535 2781 3793 3879 Size: 532 1177 467 23 335 246 1012 86 Cuts at: 3879 3891 4237 1 Size: 12 346 449 Fragments arranged by size: 1177 1012 532 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 149 1562 149 Size: 1413 3272 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 422 2635 2816 2979 422 Size: 2213 181 163 2128 Fragments arranged by size: 2213 2128 181 163 BtsI GCAGTG_nn' Cuts at: 333 958 1928 333 Size: 625 970 3090 Fragments arranged by size: 3090 970 625 ClaI AT'CG_AT Cuts at: 1196 1196 Size: 4685 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1413 1448 1413 Size: 35 4650 DraI TTT'AAA Cuts at: 1684 1684 Size: 4685 DrdI GACnn_nn'nnGTC Cuts at: 790 2391 3249 790 Size: 1601 858 2226 Fragments arranged by size: 2226 1601 858 EaeI y'GGCC_r Cuts at: 236 497 1136 2270 2444 2835 2862 4207 236 Size: 261 639 1134 174 391 27 1345 714 Fragments arranged by size: 1345 1134 714 639 391 261 174 27 EagI C'GGCC_G Cuts at: 2270 2270 Size: 4685 EarI CTCTTCn'nnn_ Cuts at: 698 1070 1082 2708 2918 698 Size: 372 12 1626 210 2465 Fragments arranged by size: 2465 1626 372 210 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2893 3203 3349 4495 2893 Size: 310 146 1146 3083 Fragments arranged by size: 3083 1146 310 146 EcoICRI GAG'CTC Cuts at: 1347 1677 1697 1347 Size: 330 20 4335 Fragments arranged by size: 4335 330 20 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 547 1103 1267 1297 1667 2510 2942 3689 3957 Size: 556 164 30 370 843 432 747 268 Cuts at: 3957 547 Size: 1275 Fragments arranged by size: 1275 843 747 556 432 370 268 164 30 EcoNI CCTnn'n_nnAGG Cuts at: 541 541 Size: 4685 EcoO109I rG'GnC_Cy Cuts at: 164 870 1515 1516 1824 164 Size: 706 645 1 308 3025 Fragments arranged by size: 3025 706 645 308 1 EcoRI G'AATT_C Cuts at: 1689 1689 Size: 4685 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2704 2704 Size: 4685 FspI TGC'GCA Cuts at: 2466 2466 Size: 4685 HaeII r_GCGC'y Cuts at: 2119 2367 3389 4368 2119 Size: 248 1022 979 2436 Fragments arranged by size: 2436 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 466 498 889 921 2527 2557 2559 2589 466 Size: 32 391 32 1606 30 2 30 2562 Fragments arranged by size: 2562 1606 391 32 32 30 30 2 HincII GTy'rAC Cuts at: 261 384 1724 261 Size: 123 1340 3222 Fragments arranged by size: 3222 1340 123 HindIII A'AGCT_T Cuts at: 1387 1387 Size: 4685 KasI G'GCGC_C Cuts at: 2115 2363 2115 Size: 248 4437 KpnI G_GTAC'C Cuts at: 1705 1705 Size: 4685 MluI A'CGCG_T Cuts at: 4118 4118 Size: 4685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 398 3356 3540 398 Size: 2958 184 1543 Fragments arranged by size: 2958 1543 184 MscI TGG'CCA Cuts at: 2446 2446 Size: 4685 MslI CAynn'nnrTG Cuts at: 1534 2674 2801 1534 Size: 1140 127 3418 Fragments arranged by size: 3418 1140 127 MspA1I CmG'CkG Cuts at: 120 431 711 1796 2110 2470 3483 3728 120 Size: 311 280 1085 314 360 1013 245 1077 Fragments arranged by size: 1085 1077 1013 360 314 311 280 245 NaeI GCC'GGC Cuts at: 1136 2866 1136 Size: 1730 2955 NarI GG'CG_CC Cuts at: 2116 2364 2116 Size: 248 4437 NcoI C'CATG_G Cuts at: 1562 1562 Size: 4685 NgoMIV G'CCGG_C Cuts at: 1134 2864 1134 Size: 1730 2955 NspI r_CATG'y Cuts at: 1738 2238 3145 1738 Size: 500 907 3278 Fragments arranged by size: 3278 907 500 PciI A'CATG_T Cuts at: 3141 3141 Size: 4685 PflMI CCAn_nnn'nTGG Cuts at: 143 143 Size: 4685 PmeI GTTT'AAAC Cuts at: 1684 1684 Size: 4685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 3824 Size: 32 4653 PspOMI G'GGCC_C Cuts at: 1515 1515 Size: 4685 PstI C_TGCA'G Cuts at: 491 812 1732 2417 491 Size: 321 920 685 2759 Fragments arranged by size: 2759 920 685 321 PvuI CG_AT'CG Cuts at: 104 104 Size: 4685 PvuII CAG'CTG Cuts at: 711 2110 2470 711 Size: 1399 360 2926 Fragments arranged by size: 2926 1399 360 RsrII CG'GwC_CG Cuts at: 950 2880 950 Size: 1930 2755 SacI G_AGCT'C Cuts at: 1349 1679 1699 1349 Size: 330 20 4335 Fragments arranged by size: 4335 330 20 SalI G'TCGA_C Cuts at: 1722 1722 Size: 4685 SapI GCTCTTCn'nnn_ Cuts at: 698 2708 2918 698 Size: 2010 210 2465 Fragments arranged by size: 2465 2010 210 SbfI CC_TGCA'GG Cuts at: 1732 1732 Size: 4685 ScaI AGT'ACT Cuts at: 4202 4202 Size: 4685 SexAI A'CCwGG_T Cuts at: 620 620 Size: 4685 SfcI C'TryA_G Cuts at: 33 487 592 808 1728 2413 3406 3597 33 Size: 454 105 216 920 685 993 191 1121 Fragments arranged by size: 1121 993 920 685 454 216 191 105 SfoI GGC'GCC Cuts at: 2117 2365 2117 Size: 248 4437 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4685 SmaI CCC'GGG Cuts at: 169 1707 169 Size: 1538 3147 SmlI C'TyrA_G Cuts at: 1840 2755 3247 3509 1840 Size: 915 492 262 3016 Fragments arranged by size: 3016 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4685 SphI G_CATG'C Cuts at: 1738 2238 1738 Size: 500 4185 StuI AGG'CCT Cuts at: 1189 1189 Size: 4685 StyI C'CwwG_G Cuts at: 722 1007 1431 1562 1819 2173 722 Size: 285 424 131 257 354 3234 Fragments arranged by size: 3234 424 354 285 257 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1668 2268 2401 2860 3069 1668 Size: 600 133 459 209 3284 Fragments arranged by size: 3284 600 459 209 133 TatI w'GTAC_w Cuts at: 422 1328 4200 422 Size: 906 2872 907 Fragments arranged by size: 2872 907 906 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 957 989 4202 4234 957 Size: 32 3213 32 1408 Fragments arranged by size: 3213 1408 32 32 Tth111I GACn'n_nGTC Cuts at: 919 2482 919 Size: 1563 3122 XbaI T'CTAG_A Cuts at: 1716 1716 Size: 4685 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1272 2180 1272 Size: 908 3777 XmaI C'CCGG_G Cuts at: 167 1705 167 Size: 1538 3147 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AloI AscI AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI BssHII BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI