Restriction map for
pF1KB9431
(Circular) (Six-base) MAPSORT of: pF1KB9431.seq Check: 691 from: 1 to: 4685
pF1KB9431 4685 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 229 460 229
Size: 231 4454
AccI GT'mk_AC
Cuts at: 1367 1723 1367
Size: 356 4329
Acc65I G'GTAC_C
Cuts at: 1701 1701
Size: 4685
AclI AA'CG_TT
Cuts at: 4525 4525
Size: 4685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 1103 1267 1667 2510 2942 3689 3957 547
Size: 556 164 400 843 432 747 268 1275
Fragments arranged by size:
1275 843 747 556 432 400 268 164
AflIII A'CryG_T
Cuts at: 2037 3141 4118 2037
Size: 1104 977 2604
Fragments arranged by size:
2604 1104 977
AgeI A'CCGG_T
Cuts at: 947 3073 947
Size: 2126 2559
AleI CACnn'nnGTG
Cuts at: 1534 1534
Size: 4685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 731 731
Size: 4685
AlwNI CAG_nnn'CTG
Cuts at: 696 744 1592 1990 3557 696
Size: 48 848 398 1567 1824
Fragments arranged by size:
1824 1567 848 398 48
ApaI G_GGCC'C
Cuts at: 1519 1519
Size: 4685
ApaLI G'TGCA_C
Cuts at: 3455 3455
Size: 4685
ApoI r'AATT_y
Cuts at: 1689 4038 4294 1689
Size: 2349 256 2080
Fragments arranged by size:
2349 2080 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4685
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
AvaI C'yCGr_G
Cuts at: 167 1411 1705 167
Size: 1244 294 3147
Fragments arranged by size:
3147 1244 294
BamHI G'GATC_C
Cuts at: 1710 1710
Size: 4685
BanI G'GyrC_C
Cuts at: 1435 1701 2115 2363 2398 1435
Size: 266 414 248 35 3722
Fragments arranged by size:
3722 414 266 248 35
BanII G_rGCy'C
Cuts at: 1007 1349 1420 1447 1519 1586 1679 1699 2729
Size: 342 71 27 72 67 93 20 1030
Cuts at: 2729 1007
Size: 2963
Fragments arranged by size:
2963 1030 342 93 72 71 67 27
20
BbeI G_GCGC'C
Cuts at: 2119 2367 2119
Size: 248 4437
BbsI GAAGACnn'nnnn_
Cuts at: 623 1160 1667 623
Size: 537 507 3641
Fragments arranged by size:
3641 537 507
BbvCI CC'TCA_GC
Cuts at: 1148 1148
Size: 4685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1206 1240 90
Size: 34 1082 34 3535
Fragments arranged by size:
3535 1082 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2583 3344 4685 2583
Size: 761 1341 2583
Fragments arranged by size:
2583 1341 761
BclI T'GATC_A
Cuts at: 252 2205 252
Size: 1953 2732
BglI GCCn_nnn'nGGC
Cuts at: 1210 1210
Size: 4685
BglII A'GATC_T
Cuts at: 2200 4237 2200
Size: 2037 2648
BlpI GC'TnA_GC
Cuts at: 1797 1797
Size: 4685
Bme1580I G_kGCm'C
Cuts at: 1031 1519 2310 2403 3459 1031
Size: 488 791 93 1056 2257
Fragments arranged by size:
2257 1056 791 488 93
BmrI ACTGGGnnnn_n'
Cuts at: 1580 1897 1978 2313 4457 1580
Size: 317 81 335 2144 1808
Fragments arranged by size:
2144 1808 335 317 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 4685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 1297
Size: 4685
Bpu10I CC'TnA_GC
Cuts at: 866 1148 866
Size: 282 4403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1861 2740 3232 3530 1861
Size: 879 492 298 3016
Fragments arranged by size:
3016 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 798 1552 4389 798
Size: 754 2837 1094
Fragments arranged by size:
2837 1094 754
BsaAI yAC'GTr
Cuts at: 2038 2038
Size: 4685
BsaBI GATnn'nnATC
Cuts at: 6 2199 4236 6
Size: 2193 2037 455
Fragments arranged by size:
2193 2037 455
BsaHI Gr'CG_yC
Cuts at: 173 434 899 1618 2116 2364 3066 4562 173
Size: 261 465 719 498 248 702 1496 296
Fragments arranged by size:
1496 719 702 498 465 296 261 248
BsaWI w'CCGG_w
Cuts at: 947 1874 2395 3073 3347 3494 3905 947
Size: 927 521 678 274 147 411 1727
Fragments arranged by size:
1727 927 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 466 496 466
Size: 30 4655
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 865 1027 1060 1063 1066 1069 1072 1075
Size: 285 162 33 3 3 3 3 3
Cuts at: 1075 1078 1081 1105 1108 1111 1114 1243 1273
Size: 3 3 24 3 3 3 129 30
Cuts at: 1273 580
Size: 3992
Fragments arranged by size:
3992 285 162 129 33 30 24 3
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 143 598 2110 2868 3445 3941 143
Size: 455 1512 758 577 496 887
Fragments arranged by size:
1512 887 758 577 496 455
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 1135 321
Size: 814 3871
BsiEI CG_ry'CG
Cuts at: 104 918 1243 2227 2273 3481 104
Size: 814 325 984 46 1208 1308
Fragments arranged by size:
1308 1208 984 814 325 46
BsiHKAI G_wGCw'C
Cuts at: 1349 1679 1699 2477 2667 3459 1349
Size: 330 20 778 190 792 2575
Fragments arranged by size:
2575 792 778 330 190 20
BsmBI CGTCTCn'nnnn_
Cuts at: 180 180
Size: 4685
Bsp1286I G_dGCh'C
Cuts at: 1007 1031 1349 1420 1447 1519 1586 1679 1699
Size: 24 318 71 27 72 67 93 20
Cuts at: 1699 2310 2403 2477 2667 2729 3459 1007
Size: 611 93 74 190 62 730 2233
Fragments arranged by size:
2233 730 611 318 190 93 93 74
72 71 67 62 27 24 20
BspEI T'CCGG_A
Cuts at: 3905 3905
Size: 4685
BspHI T'CATG_A
Cuts at: 3861 3861
Size: 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 229 460 1735 1949 2251 2632 229
Size: 231 1275 214 302 381 2282
Fragments arranged by size:
2282 1275 381 302 231 214
BsrBI CCG'CTC
Cuts at: 50 1239 1526 2977 3031 4515 4680 50
Size: 1189 287 1451 54 1484 165 55
Fragments arranged by size:
1484 1451 1189 287 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2597 2597
Size: 4685
BsrFI r'CCGG_y
Cuts at: 947 1134 2683 2864 3073 947
Size: 187 1549 181 209 2559
Fragments arranged by size:
2559 1549 209 187 181
BsrGI T'GTAC_A
Cuts at: 1328 1328
Size: 4685
BssSI C'ACGA_G
Cuts at: 2956 3314 2956
Size: 358 4327
BstAPI GCAn_nnn'nTGC
Cuts at: 985 1505 2062 985
Size: 520 557 3608
Fragments arranged by size:
3608 557 520
BstBI TT'CG_AA
Cuts at: 3046 3046
Size: 4685
BstEII G'GTnAC_C
Cuts at: 504 1566 504
Size: 1062 3623
BstYI r'GATC_y
Cuts at: 1 533 1710 2177 2200 2535 2781 3793 3879
Size: 532 1177 467 23 335 246 1012 86
Cuts at: 3879 3891 4237 1
Size: 12 346 449
Fragments arranged by size:
1177 1012 532 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 149 1562 149
Size: 1413 3272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 422 2635 2816 2979 422
Size: 2213 181 163 2128
Fragments arranged by size:
2213 2128 181 163
BtsI GCAGTG_nn'
Cuts at: 333 958 1928 333
Size: 625 970 3090
Fragments arranged by size:
3090 970 625
ClaI AT'CG_AT
Cuts at: 1196 1196
Size: 4685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1413 1448 1413
Size: 35 4650
DraI TTT'AAA
Cuts at: 1684 1684
Size: 4685
DrdI GACnn_nn'nnGTC
Cuts at: 790 2391 3249 790
Size: 1601 858 2226
Fragments arranged by size:
2226 1601 858
EaeI y'GGCC_r
Cuts at: 236 497 1136 2270 2444 2835 2862 4207 236
Size: 261 639 1134 174 391 27 1345 714
Fragments arranged by size:
1345 1134 714 639 391 261 174 27
EagI C'GGCC_G
Cuts at: 2270 2270
Size: 4685
EarI CTCTTCn'nnn_
Cuts at: 698 1070 1082 2708 2918 698
Size: 372 12 1626 210 2465
Fragments arranged by size:
2465 1626 372 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2893 3203 3349 4495 2893
Size: 310 146 1146 3083
Fragments arranged by size:
3083 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1347 1677 1697 1347
Size: 330 20 4335
Fragments arranged by size:
4335 330 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 1103 1267 1297 1667 2510 2942 3689 3957
Size: 556 164 30 370 843 432 747 268
Cuts at: 3957 547
Size: 1275
Fragments arranged by size:
1275 843 747 556 432 370 268 164
30
EcoNI CCTnn'n_nnAGG
Cuts at: 541 541
Size: 4685
EcoO109I rG'GnC_Cy
Cuts at: 164 870 1515 1516 1824 164
Size: 706 645 1 308 3025
Fragments arranged by size:
3025 706 645 308 1
EcoRI G'AATT_C
Cuts at: 1689 1689
Size: 4685
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 4685
FspI TGC'GCA
Cuts at: 2466 2466
Size: 4685
HaeII r_GCGC'y
Cuts at: 2119 2367 3389 4368 2119
Size: 248 1022 979 2436
Fragments arranged by size:
2436 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 889 921 2527 2557 2559 2589 466
Size: 32 391 32 1606 30 2 30 2562
Fragments arranged by size:
2562 1606 391 32 32 30 30 2
HincII GTy'rAC
Cuts at: 261 384 1724 261
Size: 123 1340 3222
Fragments arranged by size:
3222 1340 123
HindIII A'AGCT_T
Cuts at: 1387 1387
Size: 4685
KasI G'GCGC_C
Cuts at: 2115 2363 2115
Size: 248 4437
KpnI G_GTAC'C
Cuts at: 1705 1705
Size: 4685
MluI A'CGCG_T
Cuts at: 4118 4118
Size: 4685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 398 3356 3540 398
Size: 2958 184 1543
Fragments arranged by size:
2958 1543 184
MscI TGG'CCA
Cuts at: 2446 2446
Size: 4685
MslI CAynn'nnrTG
Cuts at: 1534 2674 2801 1534
Size: 1140 127 3418
Fragments arranged by size:
3418 1140 127
MspA1I CmG'CkG
Cuts at: 120 431 711 1796 2110 2470 3483 3728 120
Size: 311 280 1085 314 360 1013 245 1077
Fragments arranged by size:
1085 1077 1013 360 314 311 280 245
NaeI GCC'GGC
Cuts at: 1136 2866 1136
Size: 1730 2955
NarI GG'CG_CC
Cuts at: 2116 2364 2116
Size: 248 4437
NcoI C'CATG_G
Cuts at: 1562 1562
Size: 4685
NgoMIV G'CCGG_C
Cuts at: 1134 2864 1134
Size: 1730 2955
NspI r_CATG'y
Cuts at: 1738 2238 3145 1738
Size: 500 907 3278
Fragments arranged by size:
3278 907 500
PciI A'CATG_T
Cuts at: 3141 3141
Size: 4685
PflMI CCAn_nnn'nTGG
Cuts at: 143 143
Size: 4685
PmeI GTTT'AAAC
Cuts at: 1684 1684
Size: 4685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 3824
Size: 32 4653
PspOMI G'GGCC_C
Cuts at: 1515 1515
Size: 4685
PstI C_TGCA'G
Cuts at: 491 812 1732 2417 491
Size: 321 920 685 2759
Fragments arranged by size:
2759 920 685 321
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4685
PvuII CAG'CTG
Cuts at: 711 2110 2470 711
Size: 1399 360 2926
Fragments arranged by size:
2926 1399 360
RsrII CG'GwC_CG
Cuts at: 950 2880 950
Size: 1930 2755
SacI G_AGCT'C
Cuts at: 1349 1679 1699 1349
Size: 330 20 4335
Fragments arranged by size:
4335 330 20
SalI G'TCGA_C
Cuts at: 1722 1722
Size: 4685
SapI GCTCTTCn'nnn_
Cuts at: 698 2708 2918 698
Size: 2010 210 2465
Fragments arranged by size:
2465 2010 210
SbfI CC_TGCA'GG
Cuts at: 1732 1732
Size: 4685
ScaI AGT'ACT
Cuts at: 4202 4202
Size: 4685
SexAI A'CCwGG_T
Cuts at: 620 620
Size: 4685
SfcI C'TryA_G
Cuts at: 33 487 592 808 1728 2413 3406 3597 33
Size: 454 105 216 920 685 993 191 1121
Fragments arranged by size:
1121 993 920 685 454 216 191 105
SfoI GGC'GCC
Cuts at: 2117 2365 2117
Size: 248 4437
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
SmaI CCC'GGG
Cuts at: 169 1707 169
Size: 1538 3147
SmlI C'TyrA_G
Cuts at: 1840 2755 3247 3509 1840
Size: 915 492 262 3016
Fragments arranged by size:
3016 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4685
SphI G_CATG'C
Cuts at: 1738 2238 1738
Size: 500 4185
StuI AGG'CCT
Cuts at: 1189 1189
Size: 4685
StyI C'CwwG_G
Cuts at: 722 1007 1431 1562 1819 2173 722
Size: 285 424 131 257 354 3234
Fragments arranged by size:
3234 424 354 285 257 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1668 2268 2401 2860 3069 1668
Size: 600 133 459 209 3284
Fragments arranged by size:
3284 600 459 209 133
TatI w'GTAC_w
Cuts at: 422 1328 4200 422
Size: 906 2872 907
Fragments arranged by size:
2872 907 906
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 957 989 4202 4234 957
Size: 32 3213 32 1408
Fragments arranged by size:
3213 1408 32 32
Tth111I GACn'n_nGTC
Cuts at: 919 2482 919
Size: 1563 3122
XbaI T'CTAG_A
Cuts at: 1716 1716
Size: 4685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1272 2180 1272
Size: 908 3777
XmaI C'CCGG_G
Cuts at: 167 1705 167
Size: 1538 3147
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BssHII BstXI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI