Restriction map for
pF1KB9443
(Circular) (Six-base) MAPSORT of: pF1KB9443.seq Check: 6821 from: 1 to: 4034
pF1KB9443 4034 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1072 1072
Size: 4034
Acc65I G'GTAC_C
Cuts at: 1050 1050
Size: 4034
AclI AA'CG_TT
Cuts at: 3874 3874
Size: 4034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 385 625 994 1859 2291 3038 3306 250
Size: 135 240 369 865 432 747 268 978
Fragments arranged by size:
978 865 747 432 369 268 240 135
AflIII A'CryG_T
Cuts at: 1386 2490 3467 1386
Size: 1104 977 1953
Fragments arranged by size:
1953 1104 977
AgeI A'CCGG_T
Cuts at: 2422 2422
Size: 4034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 950 950
Size: 4034
AlwNI CAG_nnn'CTG
Cuts at: 1339 2906 1339
Size: 1567 2467
ApaLI G'TGCA_C
Cuts at: 2804 2804
Size: 4034
ApoI r'AATT_y
Cuts at: 1038 3387 3643 1038
Size: 2349 256 1429
Fragments arranged by size:
2349 1429 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
AvaI C'yCGr_G
Cuts at: 450 1054 450
Size: 604 3430
AvrII C'CTAG_G
Cuts at: 1014 1014
Size: 4034
BamHI G'GATC_C
Cuts at: 1059 1059
Size: 4034
BanI G'GyrC_C
Cuts at: 1050 1464 1712 1747 1050
Size: 414 248 35 3337
Fragments arranged by size:
3337 414 248 35
BanII G_rGCy'C
Cuts at: 472 760 773 916 1048 2078 472
Size: 288 13 143 132 1030 2428
Fragments arranged by size:
2428 1030 288 143 132 13
BbeI G_GCGC'C
Cuts at: 1468 1716 1468
Size: 248 3786
BbsI GAAGACnn'nnnn_
Cuts at: 705 954 705
Size: 249 3785
BciVI GTATCCnnnnn_n'
Cuts at: 1932 2693 4034 1932
Size: 761 1341 1932
Fragments arranged by size:
1932 1341 761
BclI T'GATC_A
Cuts at: 1554 1554
Size: 4034
BglI GCCn_nnn'nGGC
Cuts at: 283 283
Size: 4034
BglII A'GATC_T
Cuts at: 827 1549 3586 827
Size: 722 2037 1275
Fragments arranged by size:
2037 1275 722
BlpI GC'TnA_GC
Cuts at: 1146 1146
Size: 4034
Bme1580I G_kGCm'C
Cuts at: 115 1659 1752 2808 115
Size: 1544 93 1056 1341
Fragments arranged by size:
1544 1341 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1246 1327 1662 3806 1246
Size: 81 335 2144 1474
Fragments arranged by size:
2144 1474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 774 2401 774
Size: 1627 2407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 757
Size: 4034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1210 2089 2581 2879 826
Size: 384 879 492 298 1981
Fragments arranged by size:
1981 879 492 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 3738 3738
Size: 4034
BsaAI yAC'GTr
Cuts at: 1387 1387
Size: 4034
BsaBI GATnn'nnATC
Cuts at: 6 1548 3585 6
Size: 1542 2037 455
Fragments arranged by size:
2037 1542 455
BsaHI Gr'CG_yC
Cuts at: 520 544 1465 1713 2415 3911 520
Size: 24 921 248 702 1496 643
Fragments arranged by size:
1496 921 702 643 248 24
BsaWI w'CCGG_w
Cuts at: 140 1223 1744 2422 2696 2843 3254 140
Size: 1083 521 678 274 147 411 920
Fragments arranged by size:
1083 920 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 814 844 814
Size: 30 4004
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 462 465 735 1000 462
Size: 3 270 265 3496
Fragments arranged by size:
3496 270 265 3
BseYI C'CCAG_C
Cuts at: 133 404 419 1459 2217 2794 3290 133
Size: 271 15 1040 758 577 496 877
Fragments arranged by size:
1040 877 758 577 496 271 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 413 331
Size: 82 3952
BsiEI CG_ry'CG
Cuts at: 104 194 1576 1622 2830 104
Size: 90 1382 46 1208 1308
Fragments arranged by size:
1382 1308 1208 90 46
BsiHKAI G_wGCw'C
Cuts at: 635 773 916 1048 1826 2016 2808 635
Size: 138 143 132 778 190 792 1861
Fragments arranged by size:
1861 792 778 190 143 138 132
Bsp1286I G_dGCh'C
Cuts at: 115 472 635 760 773 916 1048 1659 1752
Size: 357 163 125 13 143 132 611 93
Cuts at: 1752 1826 2016 2078 2808 115
Size: 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 357 190 163 143 132
125 93 74 62 13
BspEI T'CCGG_A
Cuts at: 3254 3254
Size: 4034
BspHI T'CATG_A
Cuts at: 908 3210 908
Size: 2302 1732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1084 1298 1600 1981 1084
Size: 214 302 381 3137
Fragments arranged by size:
3137 381 302 214
BsrBI CCG'CTC
Cuts at: 50 656 840 986 2326 2380 3864 4029 50
Size: 606 184 146 1340 54 1484 165 55
Fragments arranged by size:
1484 1340 606 184 165 146 55 54
BsrDI GCAATG_nn'
Cuts at: 1946 1946
Size: 4034
BsrFI r'CCGG_y
Cuts at: 414 590 699 2032 2213 2422 414
Size: 176 109 1333 181 209 2026
Fragments arranged by size:
2026 1333 209 181 176 109
BssSI C'ACGA_G
Cuts at: 766 2305 2663 766
Size: 1539 358 2137
Fragments arranged by size:
2137 1539 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1411 1411
Size: 4034
BstBI TT'CG_AA
Cuts at: 2395 2395
Size: 4034
BstYI r'GATC_y
Cuts at: 1 827 1059 1526 1549 1884 2130 3142 3228
Size: 826 232 467 23 335 246 1012 86
Cuts at: 3228 3240 3586 1
Size: 12 346 449
Fragments arranged by size:
1012 826 467 449 346 335 246 232
86 23 12
BtgI C'CryG_G
Cuts at: 107 176 620 107
Size: 69 444 3521
Fragments arranged by size:
3521 444 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1984 2165 2328 1984
Size: 181 163 3690
Fragments arranged by size:
3690 181 163
BtsI GCAGTG_nn'
Cuts at: 1277 1277
Size: 4034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 138 173 138
Size: 35 3999
DraI TTT'AAA
Cuts at: 1033 1033
Size: 4034
DrdI GACnn_nn'nnGTC
Cuts at: 1740 2598 1740
Size: 858 3176
EaeI y'GGCC_r
Cuts at: 170 191 275 284 437 701 987 1619 1793
Size: 21 84 9 153 264 286 632 174
Cuts at: 1793 2184 2211 3556 170
Size: 391 27 1345 648
Fragments arranged by size:
1345 648 632 391 286 264 174 153
84 27 21 9
EagI C'GGCC_G
Cuts at: 191 1619 191
Size: 1428 2606
EarI CTCTTCn'nnn_
Cuts at: 259 2057 2267 259
Size: 1798 210 2026
Fragments arranged by size:
2026 1798 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2242 2552 2698 3844 2242
Size: 310 146 1146 2432
Fragments arranged by size:
2432 1146 310 146
EcoICRI GAG'CTC
Cuts at: 771 914 1046 771
Size: 143 132 3759
Fragments arranged by size:
3759 143 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 385 625 757 994 1859 2291 3038 3306
Size: 135 240 132 237 865 432 747 268
Cuts at: 3306 250
Size: 978
Fragments arranged by size:
978 865 747 432 268 240 237 135
132
EcoO109I rG'GnC_Cy
Cuts at: 1173 1173
Size: 4034
EcoRI G'AATT_C
Cuts at: 1038 1038
Size: 4034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 891 2053 891
Size: 1162 2872
FspI TGC'GCA
Cuts at: 643 1815 643
Size: 1172 2862
HaeII r_GCGC'y
Cuts at: 237 1468 1716 2738 3717 237
Size: 1231 248 1022 979 554
Fragments arranged by size:
1231 1022 979 554 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 1876 1906 1908 1938 727
Size: 32 1117 30 2 30 2823
Fragments arranged by size:
2823 1117 32 30 30 2
HincII GTy'rAC
Cuts at: 1073 1073
Size: 4034
KasI G'GCGC_C
Cuts at: 1464 1712 1464
Size: 248 3786
KpnI G_GTAC'C
Cuts at: 1054 1054
Size: 4034
MluI A'CGCG_T
Cuts at: 3467 3467
Size: 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 992 2705 2889 992
Size: 1713 184 2137
Fragments arranged by size:
2137 1713 184
MscI TGG'CCA
Cuts at: 1795 1795
Size: 4034
MslI CAynn'nnrTG
Cuts at: 250 2023 2150 250
Size: 1773 127 2134
Fragments arranged by size:
2134 1773 127
MspA1I CmG'CkG
Cuts at: 1145 1459 1819 2832 3077 1145
Size: 314 360 1013 245 2102
Fragments arranged by size:
2102 1013 360 314 245
NaeI GCC'GGC
Cuts at: 701 2215 701
Size: 1514 2520
NarI GG'CG_CC
Cuts at: 1465 1713 1465
Size: 248 3786
NcoI C'CATG_G
Cuts at: 107 107
Size: 4034
NgoMIV G'CCGG_C
Cuts at: 699 2213 699
Size: 1514 2520
NspI r_CATG'y
Cuts at: 726 794 1087 1587 2494 726
Size: 68 293 500 907 2266
Fragments arranged by size:
2266 907 500 293 68
PciI A'CATG_T
Cuts at: 2490 2490
Size: 4034
PmeI GTTT'AAAC
Cuts at: 1033 1033
Size: 4034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4002
PstI C_TGCA'G
Cuts at: 671 733 929 1081 1766 671
Size: 62 196 152 685 2939
Fragments arranged by size:
2939 685 196 152 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4034
PvuII CAG'CTG
Cuts at: 1459 1819 1459
Size: 360 3674
RsrII CG'GwC_CG
Cuts at: 2229 2229
Size: 4034
SacI G_AGCT'C
Cuts at: 773 916 1048 773
Size: 143 132 3759
Fragments arranged by size:
3759 143 132
SalI G'TCGA_C
Cuts at: 1071 1071
Size: 4034
SapI GCTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
SbfI CC_TGCA'GG
Cuts at: 1081 1081
Size: 4034
ScaI AGT'ACT
Cuts at: 3551 3551
Size: 4034
SfcI C'TryA_G
Cuts at: 33 667 729 925 1077 1762 2755 2946 33
Size: 634 62 196 152 685 993 191 1121
Fragments arranged by size:
1121 993 685 634 196 191 152 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 283 283
Size: 4034
SfoI GGC'GCC
Cuts at: 1466 1714 1466
Size: 248 3786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
SgrAI Cr'CCGG_yG
Cuts at: 590 590
Size: 4034
SmaI CCC'GGG
Cuts at: 1056 1056
Size: 4034
SmlI C'TyrA_G
Cuts at: 841 1189 2104 2596 2858 841
Size: 348 915 492 262 2017
Fragments arranged by size:
2017 915 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4034
SphI G_CATG'C
Cuts at: 1087 1587 1087
Size: 500 3534
StuI AGG'CCT
Cuts at: 874 1019 874
Size: 145 3889
StyI C'CwwG_G
Cuts at: 107 215 259 1014 1168 1522 107
Size: 108 44 755 154 354 2619
Fragments arranged by size:
2619 755 354 154 108 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 562 1617 1750 2209 2418 562
Size: 1055 133 459 209 2178
Fragments arranged by size:
2178 1055 459 209 133
TatI w'GTAC_w
Cuts at: 3549 3549
Size: 4034
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4002
Tth111I GACn'n_nGTC
Cuts at: 1831 1831
Size: 4034
XbaI T'CTAG_A
Cuts at: 1065 1065
Size: 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1529 1529
Size: 4034
XmaI C'CCGG_G
Cuts at: 1054 1054
Size: 4034
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI