(Circular) (Six-base) MAPSORT of: pF1KB9445.seq Check: 7657 from: 1 to: 5129 pF1KB9445 5129 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AccI GT'mk_AC Cuts at: 2167 2167 Size: 5129 Acc65I G'GTAC_C Cuts at: 2145 2145 Size: 5129 AclI AA'CG_TT Cuts at: 4969 4969 Size: 5129 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 902 2954 3386 4133 4401 902 Size: 2052 432 747 268 1630 Fragments arranged by size: 2052 1630 747 432 268 AflIII A'CryG_T Cuts at: 2481 3585 4562 2481 Size: 1104 977 3048 Fragments arranged by size: 3048 1104 977 AgeI A'CCGG_T Cuts at: 3517 3517 Size: 5129 AlwNI CAG_nnn'CTG Cuts at: 775 1372 2434 4001 775 Size: 597 1062 1567 1903 Fragments arranged by size: 1903 1567 1062 597 ApaLI G'TGCA_C Cuts at: 3899 3899 Size: 5129 ApoI r'AATT_y Cuts at: 232 347 439 1277 1730 2101 2133 4482 4738 Size: 115 92 838 453 371 32 2349 256 Cuts at: 4738 232 Size: 623 Fragments arranged by size: 2349 838 623 453 371 256 115 92 32 AseI AT'TA_AT Cuts at: 20 1219 20 Size: 1199 3930 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5129 AvaI C'yCGr_G Cuts at: 2149 2149 Size: 5129 BamHI G'GATC_C Cuts at: 2154 2154 Size: 5129 BanI G'GyrC_C Cuts at: 2145 2559 2807 2842 2145 Size: 414 248 35 4432 Fragments arranged by size: 4432 414 248 35 BanII G_rGCy'C Cuts at: 243 2143 3173 243 Size: 1900 1030 2199 Fragments arranged by size: 2199 1900 1030 BbeI G_GCGC'C Cuts at: 2563 2811 2563 Size: 248 4881 BbsI GAAGACnn'nnnn_ Cuts at: 230 1796 230 Size: 1566 3563 BciVI GTATCCnnnnn_n' Cuts at: 1682 3027 3788 5129 1682 Size: 1345 761 1341 1682 Fragments arranged by size: 1682 1345 1341 761 BclI T'GATC_A Cuts at: 1551 2649 1551 Size: 1098 4031 BfrBI ATG'CAT Cuts at: 1900 1900 Size: 5129 BglII A'GATC_T Cuts at: 335 2644 4681 335 Size: 2309 2037 783 Fragments arranged by size: 2309 2037 783 BlpI GC'TnA_GC Cuts at: 2241 2241 Size: 5129 Bme1580I G_kGCm'C Cuts at: 2754 2847 3903 2754 Size: 93 1056 3980 Fragments arranged by size: 3980 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 2341 2422 2757 4901 2341 Size: 81 335 2144 2569 Fragments arranged by size: 2569 2144 335 81 BmtI G_CTAG'C Cuts at: 809 809 Size: 5129 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3496 3496 Size: 5129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1122 1967 1122 Size: 845 4284 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1504 2305 3184 3676 3974 1504 Size: 801 879 492 298 2659 Fragments arranged by size: 2659 879 801 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4833 4833 Size: 5129 BsaAI yAC'GTr Cuts at: 2482 2482 Size: 5129 BsaBI GATnn'nnATC Cuts at: 6 2643 4680 6 Size: 2637 2037 455 Fragments arranged by size: 2637 2037 455 BsaHI Gr'CG_yC Cuts at: 2560 2808 3510 5006 2560 Size: 248 702 1496 2683 Fragments arranged by size: 2683 1496 702 248 BsaWI w'CCGG_w Cuts at: 2318 2839 3517 3791 3938 4349 2318 Size: 521 678 274 147 411 3098 Fragments arranged by size: 3098 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1654 1684 1738 1768 1654 Size: 30 54 30 5015 Fragments arranged by size: 5015 54 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1108 1111 1114 1108 Size: 3 3 5123 Fragments arranged by size: 5123 3 3 BseYI C'CCAG_C Cuts at: 298 2554 3312 3889 4385 298 Size: 2256 758 577 496 1042 Fragments arranged by size: 2256 1042 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1057 1057 Size: 5129 BsiEI CG_ry'CG Cuts at: 104 1154 2671 2717 3925 104 Size: 1050 1517 46 1208 1308 Fragments arranged by size: 1517 1308 1208 1050 46 BsiHKAI G_wGCw'C Cuts at: 2143 2921 3111 3903 2143 Size: 778 190 792 3369 Fragments arranged by size: 3369 792 778 190 Bsp1286I G_dGCh'C Cuts at: 243 2143 2754 2847 2921 3111 3173 3903 243 Size: 1900 611 93 74 190 62 730 1469 Fragments arranged by size: 1900 1469 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 4349 4349 Size: 5129 BspHI T'CATG_A Cuts at: 4305 4305 Size: 5129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1865 2179 2393 2695 3076 1865 Size: 314 214 302 381 3918 Fragments arranged by size: 3918 381 314 302 214 BsrBI CCG'CTC Cuts at: 50 3421 3475 4959 5124 50 Size: 3371 54 1484 165 55 Fragments arranged by size: 3371 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1940 3041 1940 Size: 1101 4028 BsrFI r'CCGG_y Cuts at: 3127 3308 3517 3127 Size: 181 209 4739 Fragments arranged by size: 4739 209 181 BssSI C'ACGA_G Cuts at: 3400 3758 3400 Size: 358 4771 BstAPI GCAn_nnn'nTGC Cuts at: 802 2506 802 Size: 1704 3425 BstBI TT'CG_AA Cuts at: 3490 3490 Size: 5129 BstXI CCAn_nnnn'nTGG Cuts at: 583 2024 583 Size: 1441 3688 BstYI r'GATC_y Cuts at: 1 335 1833 2154 2621 2644 2979 3225 4237 Size: 334 1498 321 467 23 335 246 1012 Cuts at: 4237 4323 4335 4681 1 Size: 86 12 346 449 Fragments arranged by size: 1498 1012 467 449 346 335 334 321 246 86 23 12 BtgI C'CryG_G Cuts at: 107 189 107 Size: 82 5047 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3079 3260 3423 3079 Size: 181 163 4785 Fragments arranged by size: 4785 181 163 BtsI GCAGTG_nn' Cuts at: 2372 2372 Size: 5129 DraI TTT'AAA Cuts at: 2067 2128 2067 Size: 61 5068 DraIII CAC_nnn'GTG Cuts at: 802 802 Size: 5129 DrdI GACnn_nn'nnGTC Cuts at: 1153 2835 3693 1153 Size: 1682 858 2589 Fragments arranged by size: 2589 1682 858 EaeI y'GGCC_r Cuts at: 186 1151 2714 2888 3279 3306 4651 186 Size: 965 1563 174 391 27 1345 664 Fragments arranged by size: 1563 1345 965 664 391 174 27 EagI C'GGCC_G Cuts at: 1151 2714 1151 Size: 1563 3566 EarI CTCTTCn'nnn_ Cuts at: 202 292 1023 1085 3152 3362 202 Size: 90 731 62 2067 210 1969 Fragments arranged by size: 2067 1969 731 210 90 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3337 3647 3793 4939 3337 Size: 310 146 1146 3527 Fragments arranged by size: 3527 1146 310 146 EcoICRI GAG'CTC Cuts at: 2141 2141 Size: 5129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 902 1122 1967 2954 3386 4133 4401 902 Size: 220 845 987 432 747 268 1630 Fragments arranged by size: 1630 987 845 747 432 268 220 EcoNI CCTnn'n_nnAGG Cuts at: 1466 1985 1466 Size: 519 4610 EcoO109I rG'GnC_Cy Cuts at: 772 2268 772 Size: 1496 3633 EcoRI G'AATT_C Cuts at: 2133 2133 Size: 5129 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1276 3148 1276 Size: 1872 3257 FspI TGC'GCA Cuts at: 2910 2910 Size: 5129 HaeII r_GCGC'y Cuts at: 2563 2811 3833 4812 2563 Size: 248 1022 979 2880 Fragments arranged by size: 2880 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 320 352 2971 3001 3003 3033 320 Size: 32 2619 30 2 30 2416 Fragments arranged by size: 2619 2416 32 30 30 2 HincII GTy'rAC Cuts at: 1965 2168 1965 Size: 203 4926 HindIII A'AGCT_T Cuts at: 1629 1852 1629 Size: 223 4906 KasI G'GCGC_C Cuts at: 2559 2807 2559 Size: 248 4881 KpnI G_GTAC'C Cuts at: 2149 2149 Size: 5129 MluI A'CGCG_T Cuts at: 4562 4562 Size: 5129 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 949 3800 3984 949 Size: 2851 184 2094 Fragments arranged by size: 2851 2094 184 MscI TGG'CCA Cuts at: 188 2890 188 Size: 2702 2427 MslI CAynn'nnrTG Cuts at: 3118 3245 3118 Size: 127 5002 MspA1I CmG'CkG Cuts at: 2240 2554 2914 3927 4172 2240 Size: 314 360 1013 245 3197 Fragments arranged by size: 3197 1013 360 314 245 NaeI GCC'GGC Cuts at: 3310 3310 Size: 5129 NarI GG'CG_CC Cuts at: 2560 2808 2560 Size: 248 4881 NcoI C'CATG_G Cuts at: 107 189 107 Size: 82 5047 NdeI CA'TA_TG Cuts at: 1010 1614 1010 Size: 604 4525 NgoMIV G'CCGG_C Cuts at: 3308 3308 Size: 5129 NheI G'CTAG_C Cuts at: 805 805 Size: 5129 NsiI A_TGCA'T Cuts at: 1902 1902 Size: 5129 NspI r_CATG'y Cuts at: 2182 2682 3589 2182 Size: 500 907 3722 Fragments arranged by size: 3722 907 500 PacI TTA_AT'TAA Cuts at: 413 413 Size: 5129 PciI A'CATG_T Cuts at: 3585 3585 Size: 5129 PmeI GTTT'AAAC Cuts at: 2128 2128 Size: 5129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4268 4300 4268 Size: 32 5097 PsiI TTA'TAA Cuts at: 325 607 2107 325 Size: 282 1500 3347 Fragments arranged by size: 3347 1500 282 PstI C_TGCA'G Cuts at: 2176 2861 2176 Size: 685 4444 PvuI CG_AT'CG Cuts at: 104 104 Size: 5129 PvuII CAG'CTG Cuts at: 2554 2914 2554 Size: 360 4769 RsrII CG'GwC_CG Cuts at: 3324 3324 Size: 5129 SacI G_AGCT'C Cuts at: 2143 2143 Size: 5129 SalI G'TCGA_C Cuts at: 2166 2166 Size: 5129 SapI GCTCTTCn'nnn_ Cuts at: 292 3152 3362 292 Size: 2860 210 2059 Fragments arranged by size: 2860 2059 210 SbfI CC_TGCA'GG Cuts at: 2176 2176 Size: 5129 ScaI AGT'ACT Cuts at: 148 2091 4646 148 Size: 1943 2555 631 Fragments arranged by size: 2555 1943 631 SfcI C'TryA_G Cuts at: 33 134 363 2172 2857 3850 4041 33 Size: 101 229 1809 685 993 191 1121 Fragments arranged by size: 1809 1121 993 685 229 191 101 SfoI GGC'GCC Cuts at: 2561 2809 2561 Size: 248 4881 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5129 SmaI CCC'GGG Cuts at: 2151 2151 Size: 5129 SmlI C'TyrA_G Cuts at: 1483 2284 3199 3691 3953 1483 Size: 801 915 492 262 2659 Fragments arranged by size: 2659 915 801 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5129 SphI G_CATG'C Cuts at: 2182 2682 2182 Size: 500 4629 SspI AAT'ATT Cuts at: 252 945 252 Size: 693 4436 StyI C'CwwG_G Cuts at: 107 189 2263 2617 107 Size: 82 2074 354 2619 Fragments arranged by size: 2619 2074 354 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 242 2712 2845 3304 3513 242 Size: 2470 133 459 209 1858 Fragments arranged by size: 2470 1858 459 209 133 TatI w'GTAC_w Cuts at: 146 2089 4644 146 Size: 1943 2555 631 Fragments arranged by size: 2555 1943 631 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4646 4678 4646 Size: 32 5097 Tth111I GACn'n_nGTC Cuts at: 2926 2926 Size: 5129 XbaI T'CTAG_A Cuts at: 2160 2160 Size: 5129 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2624 2624 Size: 5129 XmaI C'CCGG_G Cuts at: 2149 2149 Size: 5129 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI PacI PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI MfeI NotI NruI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI