Restriction map for
pF1KB9448
(Circular) (Six-base) MAPSORT of: pF1KB9448.seq Check: 10 from: 1 to: 4574
pF1KB9448 4574 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1612 1612
Size: 4574
Acc65I G'GTAC_C
Cuts at: 1590 1590
Size: 4574
AclI AA'CG_TT
Cuts at: 4414 4414
Size: 4574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1577 2399 2831 3578 3846 1577
Size: 822 432 747 268 2305
Fragments arranged by size:
2305 822 747 432 268
AflIII A'CryG_T
Cuts at: 1926 3030 4007 1926
Size: 1104 977 2493
Fragments arranged by size:
2493 1104 977
AgeI A'CCGG_T
Cuts at: 2962 2962
Size: 4574
AleI CACnn'nnGTG
Cuts at: 511 511
Size: 4574
AlwNI CAG_nnn'CTG
Cuts at: 363 587 1555 1879 3446 363
Size: 224 968 324 1567 1491
Fragments arranged by size:
1567 1491 968 324 224
ApaLI G'TGCA_C
Cuts at: 3344 3344
Size: 4574
ApoI r'AATT_y
Cuts at: 851 1578 3927 4183 851
Size: 727 2349 256 1242
Fragments arranged by size:
2349 1242 727 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4574
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4574
AvaI C'yCGr_G
Cuts at: 467 1022 1594 467
Size: 555 572 3447
Fragments arranged by size:
3447 572 555
BamHI G'GATC_C
Cuts at: 1599 1599
Size: 4574
BanI G'GyrC_C
Cuts at: 1590 2004 2252 2287 1590
Size: 414 248 35 3877
Fragments arranged by size:
3877 414 248 35
BanII G_rGCy'C
Cuts at: 646 929 1315 1588 2618 646
Size: 283 386 273 1030 2602
Fragments arranged by size:
2602 1030 386 283 273
BbeI G_GCGC'C
Cuts at: 2008 2256 2008
Size: 248 4326
BciVI GTATCCnnnnn_n'
Cuts at: 2472 3233 4574 2472
Size: 761 1341 2472
Fragments arranged by size:
2472 1341 761
BclI T'GATC_A
Cuts at: 2094 2094
Size: 4574
BglII A'GATC_T
Cuts at: 2089 4126 2089
Size: 2037 2537
BlpI GC'TnA_GC
Cuts at: 1441 1686 1441
Size: 245 4329
Bme1580I G_kGCm'C
Cuts at: 2199 2292 3348 2199
Size: 93 1056 3425
Fragments arranged by size:
3425 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 339 1451 1786 1867 2202 4346 339
Size: 1112 335 81 335 2144 567
Fragments arranged by size:
2144 1112 567 335 335 81
BmtI G_CTAG'C
Cuts at: 287 287
Size: 4574
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 4574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1750 2629 3121 3419 1750
Size: 879 492 298 2905
Fragments arranged by size:
2905 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4278 4278
Size: 4574
BsaAI yAC'GTr
Cuts at: 1927 1927
Size: 4574
BsaBI GATnn'nnATC
Cuts at: 6 830 2088 4125 6
Size: 824 1258 2037 455
Fragments arranged by size:
2037 1258 824 455
BsaHI Gr'CG_yC
Cuts at: 2005 2253 2955 4451 2005
Size: 248 702 1496 2128
Fragments arranged by size:
2128 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1763 2284 2962 3236 3383 3794 1763
Size: 521 678 274 147 411 2543
Fragments arranged by size:
2543 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 628 640 658 1102 1132 610
Size: 18 12 18 444 30 4052
Fragments arranged by size:
4052 444 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 859 1296 262
Size: 597 437 3540
Fragments arranged by size:
3540 597 437
BseYI C'CCAG_C
Cuts at: 426 630 1999 2757 3334 3830 426
Size: 204 1369 758 577 496 1170
Fragments arranged by size:
1369 1170 758 577 496 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 4574
BsiEI CG_ry'CG
Cuts at: 104 2116 2162 3370 104
Size: 2012 46 1208 1308
Fragments arranged by size:
2012 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 620 929 1315 1325 1588 2366 2556 3348 620
Size: 309 386 10 263 778 190 792 1846
Fragments arranged by size:
1846 792 778 386 309 263 190 10
BsmI GAATG_Cn'
Cuts at: 1394 1394
Size: 4574
Bsp1286I G_dGCh'C
Cuts at: 620 646 929 1315 1325 1588 2199 2292 2366
Size: 26 283 386 10 263 611 93 74
Cuts at: 2366 2556 2618 3348 620
Size: 190 62 730 1846
Fragments arranged by size:
1846 730 611 386 283 263 190 93
74 62 26 10
BspEI T'CCGG_A
Cuts at: 3794 3794
Size: 4574
BspHI T'CATG_A
Cuts at: 921 3750 921
Size: 2829 1745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1624 1838 2140 2521 1624
Size: 214 302 381 3677
Fragments arranged by size:
3677 381 302 214
BsrBI CCG'CTC
Cuts at: 50 720 2866 2920 4404 4569 50
Size: 670 2146 54 1484 165 55
Fragments arranged by size:
2146 1484 670 165 55 54
BsrDI GCAATG_nn'
Cuts at: 468 2486 468
Size: 2018 2556
BsrFI r'CCGG_y
Cuts at: 2572 2753 2962 2572
Size: 181 209 4184
Fragments arranged by size:
4184 209 181
BssSI C'ACGA_G
Cuts at: 271 2845 3203 271
Size: 2574 358 1642
Fragments arranged by size:
2574 1642 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1951 1951
Size: 4574
BstBI TT'CG_AA
Cuts at: 2935 2935
Size: 4574
BstXI CCAn_nnnn'nTGG
Cuts at: 132 208 132
Size: 76 4498
BstYI r'GATC_y
Cuts at: 1 1599 2066 2089 2424 2670 3682 3768 3780
Size: 1598 467 23 335 246 1012 86 12
Cuts at: 3780 4126 1
Size: 346 449
Fragments arranged by size:
1598 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4574
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2524 2705 2868 2524
Size: 181 163 4230
Fragments arranged by size:
4230 181 163
BtsI GCAGTG_nn'
Cuts at: 559 675 733 1817 559
Size: 116 58 1084 3316
Fragments arranged by size:
3316 1084 116 58
DraI TTT'AAA
Cuts at: 482 1499 1573 482
Size: 1017 74 3483
Fragments arranged by size:
3483 1017 74
DrdI GACnn_nn'nnGTC
Cuts at: 2280 3138 2280
Size: 858 3716
EaeI y'GGCC_r
Cuts at: 791 2159 2333 2724 2751 4096 791
Size: 1368 174 391 27 1345 1269
Fragments arranged by size:
1368 1345 1269 391 174 27
EagI C'GGCC_G
Cuts at: 2159 2159
Size: 4574
EarI CTCTTCn'nnn_
Cuts at: 239 981 1156 1313 2597 2807 239
Size: 742 175 157 1284 210 2006
Fragments arranged by size:
2006 1284 742 210 175 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2782 3092 3238 4384 2782
Size: 310 146 1146 2972
Fragments arranged by size:
2972 1146 310 146
EcoICRI GAG'CTC
Cuts at: 927 1313 1586 927
Size: 386 273 3915
Fragments arranged by size:
3915 386 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1577 2399 2831 3578 3846 1577
Size: 822 432 747 268 2305
Fragments arranged by size:
2305 822 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 123 123
Size: 4574
EcoO109I rG'GnC_Cy
Cuts at: 128 1713 128
Size: 1585 2989
EcoRI G'AATT_C
Cuts at: 1578 1578
Size: 4574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4574
FspI TGC'GCA
Cuts at: 2355 2355
Size: 4574
HaeII r_GCGC'y
Cuts at: 679 2008 2256 3278 4257 679
Size: 1329 248 1022 979 996
Fragments arranged by size:
1329 1022 996 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 317 349 628 660 1072 1104 1453 1485 2416
Size: 32 279 32 412 32 349 32 931
Cuts at: 2416 2446 2448 2478 317
Size: 30 2 30 2413
Fragments arranged by size:
2413 931 412 349 279 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1613 1613
Size: 4574
KasI G'GCGC_C
Cuts at: 2004 2252 2004
Size: 248 4326
KpnI G_GTAC'C
Cuts at: 1594 1594
Size: 4574
MluI A'CGCG_T
Cuts at: 4007 4007
Size: 4574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 225 3245 3429 225
Size: 3020 184 1370
Fragments arranged by size:
3020 1370 184
MscI TGG'CCA
Cuts at: 793 2335 793
Size: 1542 3032
MslI CAynn'nnrTG
Cuts at: 420 511 2563 2690 420
Size: 91 2052 127 2304
Fragments arranged by size:
2304 2052 127 91
MspA1I CmG'CkG
Cuts at: 147 1685 1999 2359 3372 3617 147
Size: 1538 314 360 1013 245 1104
Fragments arranged by size:
1538 1104 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2755 2755
Size: 4574
NarI GG'CG_CC
Cuts at: 2005 2253 2005
Size: 248 4326
NcoI C'CATG_G
Cuts at: 107 107
Size: 4574
NgoMIV G'CCGG_C
Cuts at: 2753 2753
Size: 4574
NheI G'CTAG_C
Cuts at: 283 283
Size: 4574
NspI r_CATG'y
Cuts at: 1276 1627 2127 3034 1276
Size: 351 500 907 2816
Fragments arranged by size:
2816 907 500 351
PasI CC'CwG_GG
Cuts at: 132 343 132
Size: 211 4363
PciI A'CATG_T
Cuts at: 3030 3030
Size: 4574
PflMI CCAn_nnn'nTGG
Cuts at: 587 587
Size: 4574
PfoI T'CCnGG_A
Cuts at: 1130 1130
Size: 4574
PmeI GTTT'AAAC
Cuts at: 1573 1573
Size: 4574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 3713
Size: 32 4542
PpuMI rG'GwC_Cy
Cuts at: 128 128
Size: 4574
PstI C_TGCA'G
Cuts at: 314 442 566 1271 1296 1621 2306 314
Size: 128 124 705 25 325 685 2582
Fragments arranged by size:
2582 705 685 325 128 124 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4574
PvuII CAG'CTG
Cuts at: 147 1999 2359 147
Size: 1852 360 2362
Fragments arranged by size:
2362 1852 360
RsrII CG'GwC_CG
Cuts at: 2769 2769
Size: 4574
SacI G_AGCT'C
Cuts at: 929 1315 1588 929
Size: 386 273 3915
Fragments arranged by size:
3915 386 273
SalI G'TCGA_C
Cuts at: 1611 1611
Size: 4574
SapI GCTCTTCn'nnn_
Cuts at: 1313 2597 2807 1313
Size: 1284 210 3080
Fragments arranged by size:
3080 1284 210
SbfI CC_TGCA'GG
Cuts at: 1621 1621
Size: 4574
ScaI AGT'ACT
Cuts at: 4091 4091
Size: 4574
SfcI C'TryA_G
Cuts at: 33 310 438 562 1267 1292 1617 2302 3295
Size: 277 128 124 705 25 325 685 993
Cuts at: 3295 3486 33
Size: 191 1121
Fragments arranged by size:
1121 993 705 685 325 277 191 128
124 25
SfoI GGC'GCC
Cuts at: 2006 2254 2006
Size: 248 4326
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4574
SmaI CCC'GGG
Cuts at: 469 1596 469
Size: 1127 3447
SmlI C'TyrA_G
Cuts at: 1022 1729 2644 3136 3398 1022
Size: 707 915 492 262 2198
Fragments arranged by size:
2198 915 707 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4574
SphI G_CATG'C
Cuts at: 1276 1627 2127 1276
Size: 351 500 3723
Fragments arranged by size:
3723 500 351
SspI AAT'ATT
Cuts at: 158 158
Size: 4574
StyI C'CwwG_G
Cuts at: 107 1004 1488 1708 2062 107
Size: 897 484 220 354 2619
Fragments arranged by size:
2619 897 484 354 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2157 2290 2749 2958 2157
Size: 133 459 209 3773
Fragments arranged by size:
3773 459 209 133
TatI w'GTAC_w
Cuts at: 4089 4089
Size: 4574
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 610 642 849 881 4091 4123 610
Size: 32 207 32 3210 32 1061
Fragments arranged by size:
3210 1061 207 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 331 2371 331
Size: 2040 2534
XbaI T'CTAG_A
Cuts at: 1605 1605
Size: 4574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 786 2069 786
Size: 1283 3291
XhoI C'TCGA_G
Cuts at: 1022 1022
Size: 4574
XmaI C'CCGG_G
Cuts at: 467 1594 467
Size: 1127 3447
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BpmI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XmnI
ZraI