Restriction map for
pF1KB9451
(Circular) (Six-base) MAPSORT of: pF1KB9451.seq Check: 1244 from: 1 to: 4109
pF1KB9451 4109 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1147 1147
Size: 4109
Acc65I G'GTAC_C
Cuts at: 1125 1125
Size: 4109
AclI AA'CG_TT
Cuts at: 3949 3949
Size: 4109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1934 2366 3113 3381 1934
Size: 432 747 268 2662
Fragments arranged by size:
2662 747 432 268
AfeI AGC'GCT
Cuts at: 577 577
Size: 4109
AflIII A'CryG_T
Cuts at: 1461 2565 3542 1461
Size: 1104 977 2028
Fragments arranged by size:
2028 1104 977
AgeI A'CCGG_T
Cuts at: 2497 2497
Size: 4109
AlwNI CAG_nnn'CTG
Cuts at: 1414 2981 1414
Size: 1567 2542
ApaI G_GGCC'C
Cuts at: 968 968
Size: 4109
ApaLI G'TGCA_C
Cuts at: 2879 2879
Size: 4109
ApoI r'AATT_y
Cuts at: 1113 3462 3718 1113
Size: 2349 256 1504
Fragments arranged by size:
2349 1504 256
AscI GG'CGCG_CC
Cuts at: 971 971
Size: 4109
AseI AT'TA_AT
Cuts at: 20 20
Size: 4109
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
AvaI C'yCGr_G
Cuts at: 394 1063 1129 394
Size: 669 66 3374
Fragments arranged by size:
3374 669 66
BamHI G'GATC_C
Cuts at: 1134 1134
Size: 4109
BanI G'GyrC_C
Cuts at: 112 265 663 756 1125 1539 1787 1822 112
Size: 153 398 93 369 414 248 35 2399
Fragments arranged by size:
2399 414 398 369 248 153 93 35
BanII G_rGCy'C
Cuts at: 323 455 890 968 1010 1123 2153 323
Size: 132 435 78 42 113 1030 2279
Fragments arranged by size:
2279 1030 435 132 113 78 42
BbeI G_GCGC'C
Cuts at: 269 667 760 1543 1791 269
Size: 398 93 783 248 2587
Fragments arranged by size:
2587 783 398 248 93
BbvCI CC'TCA_GC
Cuts at: 659 659
Size: 4109
BciVI GTATCCnnnnn_n'
Cuts at: 2007 2768 4109 2007
Size: 761 1341 2007
Fragments arranged by size:
2007 1341 761
BclI T'GATC_A
Cuts at: 1629 1629
Size: 4109
BglI GCCn_nnn'nGGC
Cuts at: 397 490 724 919 946 397
Size: 93 234 195 27 3560
Fragments arranged by size:
3560 234 195 93 27
BglII A'GATC_T
Cuts at: 1624 3661 1624
Size: 2037 2072
BlpI GC'TnA_GC
Cuts at: 604 1221 604
Size: 617 3492
Bme1580I G_kGCm'C
Cuts at: 117 968 1734 1827 2883 117
Size: 851 766 93 1056 1343
Fragments arranged by size:
1343 1056 851 766 93
BmrI ACTGGGnnnn_n'
Cuts at: 111 1321 1402 1737 3881 111
Size: 1210 81 335 2144 339
Fragments arranged by size:
2144 1210 339 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 474 969 2476 474
Size: 495 1507 2107
Fragments arranged by size:
2107 1507 495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 662
Size: 4109
Bpu10I CC'TnA_GC
Cuts at: 659 659
Size: 4109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 2164 2656 2954 1285
Size: 879 492 298 2440
Fragments arranged by size:
2440 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 547 862 3813 547
Size: 315 2951 843
Fragments arranged by size:
2951 843 315
BsaAI yAC'GTr
Cuts at: 222 1462 222
Size: 1240 2869
BsaBI GATnn'nnATC
Cuts at: 6 1623 3660 6
Size: 1617 2037 455
Fragments arranged by size:
2037 1617 455
BsaHI Gr'CG_yC
Cuts at: 266 346 664 757 1540 1788 2490 3986 266
Size: 80 318 93 783 248 702 1496 389
Fragments arranged by size:
1496 783 702 389 318 248 93 80
BsaWI w'CCGG_w
Cuts at: 1298 1819 2497 2771 2918 3329 1298
Size: 521 678 274 147 411 2078
Fragments arranged by size:
2078 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 331 489 781 799 898 916 931 1018
Size: 42 158 292 18 99 18 15 87
Cuts at: 1018 289
Size: 3380
Fragments arranged by size:
3380 292 158 99 87 42 18 18
15
BseYI C'CCAG_C
Cuts at: 544 889 1058 1086 1534 2292 2869 3365 544
Size: 345 169 28 448 758 577 496 1288
Fragments arranged by size:
1288 758 577 496 448 345 169 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1089 1089
Size: 4109
BsiEI CG_ry'CG
Cuts at: 104 254 305 329 362 380 470 494 716
Size: 150 51 24 33 18 90 24 222
Cuts at: 716 1016 1651 1697 2905 104
Size: 300 635 46 1208 1308
Fragments arranged by size:
1308 1208 635 300 222 150 90 51
46 33 24 24 18
BsiHKAI G_wGCw'C
Cuts at: 1123 1901 2091 2883 1123
Size: 778 190 792 2349
Fragments arranged by size:
2349 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 117 323 455 890 968 1010 1123 1734 1827
Size: 206 132 435 78 42 113 611 93
Cuts at: 1827 1901 2091 2153 2883 117
Size: 74 190 62 730 1343
Fragments arranged by size:
1343 730 611 435 206 190 132 113
93 78 74 62 42
BspEI T'CCGG_A
Cuts at: 3329 3329
Size: 4109
BspHI T'CATG_A
Cuts at: 3285 3285
Size: 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1159 1373 1675 2056 1159
Size: 214 302 381 3212
Fragments arranged by size:
3212 381 302 214
BsrBI CCG'CTC
Cuts at: 50 338 608 2401 2455 3939 4104 50
Size: 288 270 1793 54 1484 165 55
Fragments arranged by size:
1793 1484 288 270 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2021 2021
Size: 4109
BsrFI r'CCGG_y
Cuts at: 375 2107 2288 2497 375
Size: 1732 181 209 1987
Fragments arranged by size:
1987 1732 209 181
BssHII G'CGCG_C
Cuts at: 971 971
Size: 4109
BssSI C'ACGA_G
Cuts at: 2380 2738 2380
Size: 358 3751
BstAPI GCAn_nnn'nTGC
Cuts at: 1486 1486
Size: 4109
BstBI TT'CG_AA
Cuts at: 2470 2470
Size: 4109
BstXI CCAn_nnnn'nTGG
Cuts at: 897 897
Size: 4109
BstYI r'GATC_y
Cuts at: 1 1134 1601 1624 1959 2205 3217 3303 3315
Size: 1133 467 23 335 246 1012 86 12
Cuts at: 3315 3661 1
Size: 346 449
Fragments arranged by size:
1133 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 152 248 323 356 431 464 470 998
Size: 45 96 75 33 75 33 6 528
Cuts at: 998 1010 107
Size: 12 3206
Fragments arranged by size:
3206 528 96 75 75 45 33 33
12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2059 2240 2403 2059
Size: 181 163 3765
Fragments arranged by size:
3765 181 163
BtsI GCAGTG_nn'
Cuts at: 1352 1352
Size: 4109
DraI TTT'AAA
Cuts at: 1108 1108
Size: 4109
DrdI GACnn_nn'nnGTC
Cuts at: 1815 2673 1815
Size: 858 3251
EaeI y'GGCC_r
Cuts at: 216 251 302 326 359 377 467 491 713
Size: 35 51 24 33 18 90 24 222
Cuts at: 713 1013 1694 1868 2259 2286 3631 216
Size: 300 681 174 391 27 1345 694
Fragments arranged by size:
1345 694 681 391 300 222 174 90
51 35 33 27 24 24 18
EagI C'GGCC_G
Cuts at: 251 302 326 359 377 467 491 713 1013
Size: 51 24 33 18 90 24 222 300
Cuts at: 1013 1694 251
Size: 681 2666
Fragments arranged by size:
2666 681 300 222 90 51 33 24
24 18
EarI CTCTTCn'nnn_
Cuts at: 2132 2342 2132
Size: 210 3899
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 945 2317 2627 2773 3919 945
Size: 1372 310 146 1146 1135
Fragments arranged by size:
1372 1146 1135 310 146
EcoICRI GAG'CTC
Cuts at: 1121 1121
Size: 4109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 1934 2366 3113 3381 662
Size: 1272 432 747 268 1390
Fragments arranged by size:
1390 1272 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 426 948 1248 426
Size: 522 300 3287
Fragments arranged by size:
3287 522 300
EcoRI G'AATT_C
Cuts at: 1113 1113
Size: 4109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 594 2128 594
Size: 1534 2575
FspI TGC'GCA
Cuts at: 1038 1890 1038
Size: 852 3257
HaeII r_GCGC'y
Cuts at: 269 299 579 667 760 935 1543 1791 2813
Size: 30 280 88 93 175 608 248 1022
Cuts at: 2813 3792 269
Size: 979 586
Fragments arranged by size:
1022 979 608 586 280 248 175 93
88 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 1951 1981 1983 2013 472
Size: 32 1447 30 2 30 2568
Fragments arranged by size:
2568 1447 32 30 30 2
HincII GTy'rAC
Cuts at: 1148 1148
Size: 4109
KasI G'GCGC_C
Cuts at: 265 663 756 1539 1787 265
Size: 398 93 783 248 2587
Fragments arranged by size:
2587 783 398 248 93
KpnI G_GTAC'C
Cuts at: 1129 1129
Size: 4109
MluI A'CGCG_T
Cuts at: 3542 3542
Size: 4109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2780 2964 2780
Size: 184 3925
MscI TGG'CCA
Cuts at: 218 1870 218
Size: 1652 2457
MslI CAynn'nnrTG
Cuts at: 2098 2225 2098
Size: 127 3982
MspA1I CmG'CkG
Cuts at: 154 250 325 358 388 433 472 670 709
Size: 96 75 33 30 45 39 198 39
Cuts at: 709 754 778 835 1000 1012 1090 1220 1534
Size: 45 24 57 165 12 78 130 314
Cuts at: 1534 1894 2907 3152 154
Size: 360 1013 245 1111
Fragments arranged by size:
1111 1013 360 314 245 198 165 130
96 78 75 57 45 45 39 39
33 30 24 12
NaeI GCC'GGC
Cuts at: 377 2290 377
Size: 1913 2196
NarI GG'CG_CC
Cuts at: 266 664 757 1540 1788 266
Size: 398 93 783 248 2587
Fragments arranged by size:
2587 783 398 248 93
NcoI C'CATG_G
Cuts at: 107 107
Size: 4109
NgoMIV G'CCGG_C
Cuts at: 375 2288 375
Size: 1913 2196
NotI GC'GGCC_GC
Cuts at: 251 326 713 1013 251
Size: 75 387 300 3347
Fragments arranged by size:
3347 387 300 75
NspI r_CATG'y
Cuts at: 1162 1662 2569 1162
Size: 500 907 2702
Fragments arranged by size:
2702 907 500
PciI A'CATG_T
Cuts at: 2565 2565
Size: 4109
PmeI GTTT'AAAC
Cuts at: 1108 1108
Size: 4109
PmlI CAC'GTG
Cuts at: 222 222
Size: 4109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 4077
PspOMI G'GGCC_C
Cuts at: 964 964
Size: 4109
PstI C_TGCA'G
Cuts at: 284 1156 1841 284
Size: 872 685 2552
Fragments arranged by size:
2552 872 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4109
PvuII CAG'CTG
Cuts at: 709 1534 1894 709
Size: 825 360 2924
Fragments arranged by size:
2924 825 360
RsrII CG'GwC_CG
Cuts at: 2304 2304
Size: 4109
SacI G_AGCT'C
Cuts at: 1123 1123
Size: 4109
SacII CC_GC'GG
Cuts at: 155 251 326 359 434 473 1001 1013 155
Size: 96 75 33 75 39 528 12 3251
Fragments arranged by size:
3251 528 96 75 75 39 33 12
SalI G'TCGA_C
Cuts at: 1146 1146
Size: 4109
SapI GCTCTTCn'nnn_
Cuts at: 2132 2342 2132
Size: 210 3899
SbfI CC_TGCA'GG
Cuts at: 1156 1156
Size: 4109
ScaI AGT'ACT
Cuts at: 3626 3626
Size: 4109
SfcI C'TryA_G
Cuts at: 33 280 1152 1837 2830 3021 33
Size: 247 872 685 993 191 1121
Fragments arranged by size:
1121 993 872 685 247 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 490 919 490
Size: 429 3680
SfoI GGC'GCC
Cuts at: 267 665 758 1541 1789 267
Size: 398 93 783 248 2587
Fragments arranged by size:
2587 783 398 248 93
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
SmaI CCC'GGG
Cuts at: 1131 1131
Size: 4109
SmlI C'TyrA_G
Cuts at: 1264 2179 2671 2933 1264
Size: 915 492 262 2440
Fragments arranged by size:
2440 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4109
SphI G_CATG'C
Cuts at: 1162 1662 1162
Size: 500 3609
StuI AGG'CCT
Cuts at: 162 162
Size: 4109
StyI C'CwwG_G
Cuts at: 107 269 515 872 1243 1597 107
Size: 162 246 357 371 354 2619
Fragments arranged by size:
2619 371 357 354 246 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 1692 1825 2284 2493 100
Size: 1592 133 459 209 1716
Fragments arranged by size:
1716 1592 459 209 133
TatI w'GTAC_w
Cuts at: 3624 3624
Size: 4109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4077
Tth111I GACn'n_nGTC
Cuts at: 1906 1906
Size: 4109
XbaI T'CTAG_A
Cuts at: 1140 1140
Size: 4109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1604 1604
Size: 4109
XmaI C'CCGG_G
Cuts at: 1129 1129
Size: 4109
XmnI GAAnn'nnTTC
Cuts at: 595 595
Size: 4109
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PflMI PfoI
PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI