Restriction map for
pF1KB9453
(Circular) (Six-base) MAPSORT of: pF1KB9453.seq Check: 5545 from: 1 to: 5030
pF1KB9453 5030 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 492 492
Size: 5030
AccI GT'mk_AC
Cuts at: 1186 2068 1186
Size: 882 4148
Acc65I G'GTAC_C
Cuts at: 2046 2046
Size: 5030
AclI AA'CG_TT
Cuts at: 2024 4870 2024
Size: 2846 2184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 805 959 1211 2855 3287 4034 4302 619
Size: 186 154 252 1644 432 747 268 1347
Fragments arranged by size:
1644 1347 747 432 268 252 186 154
AfeI AGC'GCT
Cuts at: 1713 1713
Size: 5030
AflII C'TTAA_G
Cuts at: 891 891
Size: 5030
AflIII A'CryG_T
Cuts at: 508 510 2382 3486 4463 508
Size: 2 1872 1104 977 1075
Fragments arranged by size:
1872 1104 1075 977 2
AgeI A'CCGG_T
Cuts at: 3418 3418
Size: 5030
AleI CACnn'nnGTG
Cuts at: 937 1804 937
Size: 867 4163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1772 1772
Size: 5030
AlwNI CAG_nnn'CTG
Cuts at: 1090 1458 2335 3902 1090
Size: 368 877 1567 2218
Fragments arranged by size:
2218 1567 877 368
ApaI G_GGCC'C
Cuts at: 584 1277 1414 1851 584
Size: 693 137 437 3763
Fragments arranged by size:
3763 693 437 137
ApaLI G'TGCA_C
Cuts at: 3800 3800
Size: 5030
ApoI r'AATT_y
Cuts at: 985 1215 2034 4383 4639 985
Size: 230 819 2349 256 1376
Fragments arranged by size:
2349 1376 819 256 230
AseI AT'TA_AT
Cuts at: 20 20
Size: 5030
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
AvaI C'yCGr_G
Cuts at: 242 395 1819 2050 242
Size: 153 1424 231 3222
Fragments arranged by size:
3222 1424 231 153
BamHI G'GATC_C
Cuts at: 2055 2055
Size: 5030
BanI G'GyrC_C
Cuts at: 1427 1806 2046 2460 2708 2743 1427
Size: 379 240 414 248 35 3714
Fragments arranged by size:
3714 414 379 248 240 35
BanII G_rGCy'C
Cuts at: 362 584 910 1099 1277 1414 1480 1779 1851
Size: 222 326 189 178 137 66 299 72
Cuts at: 1851 2044 3074 362
Size: 193 1030 2318
Fragments arranged by size:
2318 1030 326 299 222 193 189 178
137 72 66
BbeI G_GCGC'C
Cuts at: 2464 2712 2464
Size: 248 4782
BbsI GAAGACnn'nnnn_
Cuts at: 202 290 881 202
Size: 88 591 4351
Fragments arranged by size:
4351 591 88
BciVI GTATCCnnnnn_n'
Cuts at: 2928 3689 5030 2928
Size: 761 1341 2928
Fragments arranged by size:
2928 1341 761
BclI T'GATC_A
Cuts at: 2550 2550
Size: 5030
BfrBI ATG'CAT
Cuts at: 1254 1254
Size: 5030
BglII A'GATC_T
Cuts at: 2545 4582 2545
Size: 2037 2993
BlpI GC'TnA_GC
Cuts at: 2142 2142
Size: 5030
Bme1580I G_kGCm'C
Cuts at: 584 1277 1414 1430 1811 1851 2655 2748 3804
Size: 693 137 16 381 40 804 93 1056
Cuts at: 3804 584
Size: 1810
Fragments arranged by size:
1810 1056 804 693 381 137 93 40
16
BmrI ACTGGGnnnn_n'
Cuts at: 1075 1556 2242 2323 2658 4802 1075
Size: 481 686 81 335 2144 1303
Fragments arranged by size:
2144 1303 686 481 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3397 3397
Size: 5030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 788 1754 1979 485
Size: 303 966 225 3536
Fragments arranged by size:
3536 966 303 225
Bpu10I CC'TnA_GC
Cuts at: 356 1016 356
Size: 660 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2206 3085 3577 3875 2206
Size: 879 492 298 3361
Fragments arranged by size:
3361 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 305 907 4734 305
Size: 602 3827 601
Fragments arranged by size:
3827 602 601
BsaAI yAC'GTr
Cuts at: 511 2383 511
Size: 1872 3158
BsaBI GATnn'nnATC
Cuts at: 6 634 2544 4581 6
Size: 628 1910 2037 455
Fragments arranged by size:
2037 1910 628 455
BsaHI Gr'CG_yC
Cuts at: 2461 2709 3411 4907 2461
Size: 248 702 1496 2584
Fragments arranged by size:
2584 1496 702 248
BsaWI w'CCGG_w
Cuts at: 115 468 2219 2740 3418 3692 3839 4250 115
Size: 353 1751 521 678 274 147 411 895
Fragments arranged by size:
1751 895 678 521 411 353 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 177 442 1430 1901 1904 177
Size: 265 988 471 3 3303
Fragments arranged by size:
3303 988 471 265 3
BseYI C'CCAG_C
Cuts at: 1083 1312 1645 2455 3213 3790 4286 1083
Size: 229 333 810 758 577 496 1827
Fragments arranged by size:
1827 810 758 577 496 333 229
BsiEI CG_ry'CG
Cuts at: 104 116 2572 2618 3826 104
Size: 12 2456 46 1208 1308
Fragments arranged by size:
2456 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 910 2044 2822 3012 3804 910
Size: 1134 778 190 792 2136
Fragments arranged by size:
2136 1134 792 778 190
BsmI GAATG_Cn'
Cuts at: 218 218
Size: 5030
BsmBI CGTCTCn'nnnn_
Cuts at: 1181 1181
Size: 5030
Bsp1286I G_dGCh'C
Cuts at: 362 584 910 1099 1277 1414 1430 1480 1779
Size: 222 326 189 178 137 16 50 299
Cuts at: 1779 1811 1851 2044 2655 2748 2822 3012 3074
Size: 32 40 193 611 93 74 190 62
Cuts at: 3074 3804 362
Size: 730 1588
Fragments arranged by size:
1588 730 611 326 299 222 193 190
189 178 137 93 74 62 50 40
32 16
BspEI T'CCGG_A
Cuts at: 468 4250 468
Size: 3782 1248
BspHI T'CATG_A
Cuts at: 527 4206 527
Size: 3679 1351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 492 2080 2294 2596 2977 492
Size: 1588 214 302 381 2545
Fragments arranged by size:
2545 1588 381 302 214
BsrBI CCG'CTC
Cuts at: 50 432 3322 3376 4860 5025 50
Size: 382 2890 54 1484 165 55
Fragments arranged by size:
2890 1484 382 165 55 54
BsrDI GCAATG_nn'
Cuts at: 627 968 2942 627
Size: 341 1974 2715
Fragments arranged by size:
2715 1974 341
BsrFI r'CCGG_y
Cuts at: 1885 3028 3209 3418 1885
Size: 1143 181 209 3497
Fragments arranged by size:
3497 1143 209 181
BssSI C'ACGA_G
Cuts at: 1707 3301 3659 1707
Size: 1594 358 3078
Fragments arranged by size:
3078 1594 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2407 2407
Size: 5030
BstBI TT'CG_AA
Cuts at: 3391 3391
Size: 5030
BstXI CCAn_nnnn'nTGG
Cuts at: 135 741 997 1125 1376 1423 135
Size: 606 256 128 251 47 3742
Fragments arranged by size:
3742 606 256 251 128 47
BstYI r'GATC_y
Cuts at: 1 887 2055 2522 2545 2880 3126 4138 4224
Size: 886 1168 467 23 335 246 1012 86
Cuts at: 4224 4236 4582 1
Size: 12 346 449
Fragments arranged by size:
1168 1012 886 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 335 335
Size: 5030
BtgI C'CryG_G
Cuts at: 107 1245 1431 1603 1678 1735 1925 107
Size: 1138 186 172 75 57 190 3212
Fragments arranged by size:
3212 1138 190 186 172 75 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2980 3161 3324 2980
Size: 181 163 4686
Fragments arranged by size:
4686 181 163
BtsI GCAGTG_nn'
Cuts at: 767 2273 767
Size: 1506 3524
DraI TTT'AAA
Cuts at: 2029 2029
Size: 5030
DraIII CAC_nnn'GTG
Cuts at: 941 941
Size: 5030
DrdI GACnn_nn'nnGTC
Cuts at: 2736 3594 2736
Size: 858 4172
EaeI y'GGCC_r
Cuts at: 684 963 1127 2615 2789 3180 3207 4552 684
Size: 279 164 1488 174 391 27 1345 1162
Fragments arranged by size:
1488 1345 1162 391 279 174 164 27
EagI C'GGCC_G
Cuts at: 2615 2615
Size: 5030
EarI CTCTTCn'nnn_
Cuts at: 154 419 1601 3053 3263 154
Size: 265 1182 1452 210 1921
Fragments arranged by size:
1921 1452 1182 265 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1619 1631 1850 1872 1907 3238 3548 3694 4840
Size: 12 219 22 35 1331 310 146 1146
Cuts at: 4840 1619
Size: 1809
Fragments arranged by size:
1809 1331 1146 310 219 146 35 22
12
EcoICRI GAG'CTC
Cuts at: 908 2042 908
Size: 1134 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 619 788 805 959 1211 1754 1979 2855
Size: 134 169 17 154 252 543 225 876
Cuts at: 2855 3287 4034 4302 485
Size: 432 747 268 1213
Fragments arranged by size:
1213 876 747 543 432 268 252 225
169 154 134 17
EcoNI CCTnn'n_nnAGG
Cuts at: 787 787
Size: 5030
EcoO109I rG'GnC_Cy
Cuts at: 344 863 1410 1411 1847 1848 2169 344
Size: 519 547 1 436 1 321 3205
Fragments arranged by size:
3205 547 519 436 321 1 1
EcoRI G'AATT_C
Cuts at: 1215 2034 1215
Size: 819 4211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3049 3049
Size: 5030
FspI TGC'GCA
Cuts at: 304 2811 304
Size: 2507 2523
HaeII r_GCGC'y
Cuts at: 1715 2464 2712 3734 4713 1715
Size: 749 248 1022 979 2032
Fragments arranged by size:
2032 1022 979 749 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 994 1026 2872 2902 2904 2934 328
Size: 32 634 32 1846 30 2 30 2424
Fragments arranged by size:
2424 1846 634 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2069 2069
Size: 5030
KasI G'GCGC_C
Cuts at: 2460 2708 2460
Size: 248 4782
KpnI G_GTAC'C
Cuts at: 2050 2050
Size: 5030
MluI A'CGCG_T
Cuts at: 4463 4463
Size: 5030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 116 3701 3885 116
Size: 3585 184 1261
Fragments arranged by size:
3585 1261 184
MscI TGG'CCA
Cuts at: 686 965 1129 2791 686
Size: 279 164 1662 2925
Fragments arranged by size:
2925 1662 279 164
MslI CAynn'nnrTG
Cuts at: 451 937 1259 1804 1951 1963 3019 3146 451
Size: 486 322 545 147 12 1056 127 2335
Fragments arranged by size:
2335 1056 545 486 322 147 127 12
MspA1I CmG'CkG
Cuts at: 170 2141 2455 2815 3828 4073 170
Size: 1971 314 360 1013 245 1127
Fragments arranged by size:
1971 1127 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1887 3211 1887
Size: 1324 3706
NarI GG'CG_CC
Cuts at: 2461 2709 2461
Size: 248 4782
NcoI C'CATG_G
Cuts at: 107 1431 1603 1678 1735 1925 107
Size: 1324 172 75 57 190 3212
Fragments arranged by size:
3212 1324 190 172 75 57
NgoMIV G'CCGG_C
Cuts at: 1885 3209 1885
Size: 1324 3706
NsiI A_TGCA'T
Cuts at: 1256 1256
Size: 5030
NspI r_CATG'y
Cuts at: 1254 1977 2083 2583 3490 1254
Size: 723 106 500 907 2794
Fragments arranged by size:
2794 907 723 500 106
PciI A'CATG_T
Cuts at: 3486 3486
Size: 5030
PflMI CCAn_nnn'nTGG
Cuts at: 1876 1876
Size: 5030
PfoI T'CCnGG_A
Cuts at: 207 1770 207
Size: 1563 3467
PmeI GTTT'AAAC
Cuts at: 2029 2029
Size: 5030
PmlI CAC'GTG
Cuts at: 511 511
Size: 5030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 4169
Size: 32 4998
PpuMI rG'GwC_Cy
Cuts at: 344 863 344
Size: 519 4511
PspOMI G'GGCC_C
Cuts at: 580 1273 1410 1847 580
Size: 693 137 437 3763
Fragments arranged by size:
3763 693 437 137
PstI C_TGCA'G
Cuts at: 278 489 2077 2762 278
Size: 211 1588 685 2546
Fragments arranged by size:
2546 1588 685 211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5030
PvuII CAG'CTG
Cuts at: 2455 2815 2455
Size: 360 4670
RsrII CG'GwC_CG
Cuts at: 3225 3225
Size: 5030
SacI G_AGCT'C
Cuts at: 910 2044 910
Size: 1134 3896
SalI G'TCGA_C
Cuts at: 2067 2067
Size: 5030
SanDI GG'GwC_CC
Cuts at: 863 863
Size: 5030
SapI GCTCTTCn'nnn_
Cuts at: 3053 3263 3053
Size: 210 4820
SbfI CC_TGCA'GG
Cuts at: 2077 2077
Size: 5030
ScaI AGT'ACT
Cuts at: 4547 4547
Size: 5030
SfcI C'TryA_G
Cuts at: 33 218 274 485 2073 2758 3751 3942 33
Size: 185 56 211 1588 685 993 191 1121
Fragments arranged by size:
1588 1121 993 685 211 191 185 56
SfoI GGC'GCC
Cuts at: 2462 2710 2462
Size: 248 4782
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
SmaI CCC'GGG
Cuts at: 1821 2052 1821
Size: 231 4799
SmlI C'TyrA_G
Cuts at: 242 891 2185 3100 3592 3854 242
Size: 649 1294 915 492 262 1418
Fragments arranged by size:
1418 1294 915 649 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5030
SphI G_CATG'C
Cuts at: 1254 1977 2083 2583 1254
Size: 723 106 500 3701
Fragments arranged by size:
3701 723 500 106
SspI AAT'ATT
Cuts at: 1045 1045
Size: 5030
StuI AGG'CCT
Cuts at: 996 996
Size: 5030
StyI C'CwwG_G
Cuts at: 107 1375 1431 1603 1678 1735 1925 2164 2518
Size: 1268 56 172 75 57 190 239 354
Cuts at: 2518 107
Size: 2619
Fragments arranged by size:
2619 1268 354 239 190 172 75 57
56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1057 1207 1319 2613 2746 3205 3414 1057
Size: 150 112 1294 133 459 209 2673
Fragments arranged by size:
2673 1294 459 209 150 133 112
TatI w'GTAC_w
Cuts at: 4545 4545
Size: 5030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4547 4579 4547
Size: 32 4998
Tth111I GACn'n_nGTC
Cuts at: 2827 2827
Size: 5030
XbaI T'CTAG_A
Cuts at: 2061 2061
Size: 5030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1122 1252 1653 1956 2525 1122
Size: 130 401 303 569 3627
Fragments arranged by size:
3627 569 401 303 130
XhoI C'TCGA_G
Cuts at: 242 242
Size: 5030
XmaI C'CCGG_G
Cuts at: 1819 2050 1819
Size: 231 4799
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AhdI AloI AscI AvrII BaeI BbvCI BcgI
BglI BmgBI BmtI BsaXI BsgI BsiWI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SwaI XmnI ZraI