Restriction map for
pF1KB9454
(Circular) (Six-base) MAPSORT of: pF1KB9454.seq Check: 4485 from: 1 to: 4670
pF1KB9454 4670 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 158 1708 158
Size: 1550 3120
Acc65I G'GTAC_C
Cuts at: 1686 1686
Size: 4670
AclI AA'CG_TT
Cuts at: 4510 4510
Size: 4670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 905 2495 2927 3674 3942 199
Size: 706 1590 432 747 268 927
Fragments arranged by size:
1590 927 747 706 432 268
AflIII A'CryG_T
Cuts at: 1177 2022 3126 4103 1177
Size: 845 1104 977 1744
Fragments arranged by size:
1744 1104 977 845
AgeI A'CCGG_T
Cuts at: 3058 3058
Size: 4670
AleI CACnn'nnGTG
Cuts at: 1132 1132
Size: 4670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1432 1509 1432
Size: 77 4593
AlwNI CAG_nnn'CTG
Cuts at: 1975 3542 1975
Size: 1567 3103
ApaLI G'TGCA_C
Cuts at: 3440 3440
Size: 4670
ApoI r'AATT_y
Cuts at: 558 1674 4023 4279 558
Size: 1116 2349 256 949
Fragments arranged by size:
2349 1116 949 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4670
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
AvaI C'yCGr_G
Cuts at: 1690 1690
Size: 4670
BamHI G'GATC_C
Cuts at: 1695 1695
Size: 4670
BanI G'GyrC_C
Cuts at: 1686 2100 2348 2383 1686
Size: 414 248 35 3973
Fragments arranged by size:
3973 414 248 35
BanII G_rGCy'C
Cuts at: 1019 1470 1684 2714 1019
Size: 451 214 1030 2975
Fragments arranged by size:
2975 1030 451 214
BbeI G_GCGC'C
Cuts at: 2104 2352 2104
Size: 248 4422
BbsI GAAGACnn'nnnn_
Cuts at: 218 1174 218
Size: 956 3714
BbvCI CC'TCA_GC
Cuts at: 893 893
Size: 4670
BciVI GTATCCnnnnn_n'
Cuts at: 2568 3329 4670 2568
Size: 761 1341 2568
Fragments arranged by size:
2568 1341 761
BclI T'GATC_A
Cuts at: 2190 2190
Size: 4670
BglII A'GATC_T
Cuts at: 1413 1578 2185 4222 1413
Size: 165 607 2037 1861
Fragments arranged by size:
2037 1861 607 165
BlpI GC'TnA_GC
Cuts at: 1782 1782
Size: 4670
Bme1580I G_kGCm'C
Cuts at: 2295 2388 3444 2295
Size: 93 1056 3521
Fragments arranged by size:
3521 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 829 1063 1882 1963 2298 4442 829
Size: 234 819 81 335 2144 1057
Fragments arranged by size:
2144 1057 819 335 234 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3037 3037
Size: 4670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 884 722
Size: 162 4508
Bpu10I CC'TnA_GC
Cuts at: 760 893 760
Size: 133 4537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1196 1233 1846 2725 3217 3515 1196
Size: 37 613 879 492 298 2351
Fragments arranged by size:
2351 879 613 492 298 37
BsaI GGTCTCn'nnnn_
Cuts at: 929 4374 929
Size: 3445 1225
BsaAI yAC'GTr
Cuts at: 1338 2023 1338
Size: 685 3985
BsaBI GATnn'nnATC
Cuts at: 6 2184 4221 6
Size: 2178 2037 455
Fragments arranged by size:
2178 2037 455
BsaHI Gr'CG_yC
Cuts at: 2101 2349 3051 4547 2101
Size: 248 702 1496 2224
Fragments arranged by size:
2224 1496 702 248
BsaWI w'CCGG_w
Cuts at: 114 1859 2380 3058 3332 3479 3890 114
Size: 1745 521 678 274 147 411 894
Fragments arranged by size:
1745 894 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 695 725 857 887 926 956 695
Size: 30 132 30 39 30 4409
Fragments arranged by size:
4409 132 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 444 462 820 444
Size: 18 358 4294
Fragments arranged by size:
4294 358 18
BseYI C'CCAG_C
Cuts at: 1602 2095 2853 3430 3926 1602
Size: 493 758 577 496 2346
Fragments arranged by size:
2346 758 577 496 493
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 794
Size: 4670
BsiEI CG_ry'CG
Cuts at: 104 2212 2258 3466 104
Size: 2108 46 1208 1308
Fragments arranged by size:
2108 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 878 1470 1684 2462 2652 3444 878
Size: 592 214 778 190 792 2104
Fragments arranged by size:
2104 792 778 592 214 190
BsmI GAATG_Cn'
Cuts at: 1111 1111
Size: 4670
Bsp1286I G_dGCh'C
Cuts at: 878 1019 1470 1684 2295 2388 2462 2652 2714
Size: 141 451 214 611 93 74 190 62
Cuts at: 2714 3444 878
Size: 730 2104
Fragments arranged by size:
2104 730 611 451 214 190 141 93
74 62
BspEI T'CCGG_A
Cuts at: 3890 3890
Size: 4670
BspHI T'CATG_A
Cuts at: 3846 3846
Size: 4670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 1720 1934 2236 2617 766
Size: 954 214 302 381 2819
Fragments arranged by size:
2819 954 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2962 3016 4500 4665 50
Size: 2912 54 1484 165 55
Fragments arranged by size:
2912 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2582 2582
Size: 4670
BsrFI r'CCGG_y
Cuts at: 2668 2849 3058 2668
Size: 181 209 4280
Fragments arranged by size:
4280 209 181
BsrGI T'GTAC_A
Cuts at: 615 615
Size: 4670
BssSI C'ACGA_G
Cuts at: 2941 3299 2941
Size: 358 4312
BstAPI GCAn_nnn'nTGC
Cuts at: 2047 2047
Size: 4670
BstBI TT'CG_AA
Cuts at: 3031 3031
Size: 4670
BstYI r'GATC_y
Cuts at: 1 1413 1578 1695 2162 2185 2520 2766 3778
Size: 1412 165 117 467 23 335 246 1012
Cuts at: 3778 3864 3876 4222 1
Size: 86 12 346 449
Fragments arranged by size:
1412 1012 467 449 346 335 246 165
117 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 990 2620 2801 2964 990
Size: 1630 181 163 2696
Fragments arranged by size:
2696 1630 181 163
BtsI GCAGTG_nn'
Cuts at: 1617 1913 1617
Size: 296 4374
DraI TTT'AAA
Cuts at: 198 1669 198
Size: 1471 3199
DrdI GACnn_nn'nnGTC
Cuts at: 1570 2376 3234 1570
Size: 806 858 3006
Fragments arranged by size:
3006 858 806
EaeI y'GGCC_r
Cuts at: 2255 2429 2820 2847 4192 2255
Size: 174 391 27 1345 2733
Fragments arranged by size:
2733 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2255 2255
Size: 4670
EarI CTCTTCn'nnn_
Cuts at: 444 462 471 480 489 495 513 691 2693
Size: 18 9 9 9 6 18 178 2002
Cuts at: 2693 2903 444
Size: 210 2211
Fragments arranged by size:
2211 2002 210 178 18 18 9 9
9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 321 2878 3188 3334 4480 321
Size: 2557 310 146 1146 511
Fragments arranged by size:
2557 1146 511 310 146
EcoICRI GAG'CTC
Cuts at: 1468 1682 1468
Size: 214 4456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 722 884 905 2495 2927 3674 3942 199
Size: 523 162 21 1590 432 747 268 927
Fragments arranged by size:
1590 927 747 523 432 268 162 21
EcoO109I rG'GnC_Cy
Cuts at: 1809 1809
Size: 4670
EcoRI G'AATT_C
Cuts at: 1674 1674
Size: 4670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 4670
FspI TGC'GCA
Cuts at: 2451 2451
Size: 4670
HaeII r_GCGC'y
Cuts at: 2104 2352 3374 4353 2104
Size: 248 1022 979 2421
Fragments arranged by size:
2421 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 224 256 421 453 926 958 2512 2542 2544
Size: 32 165 32 473 32 1554 30 2
Cuts at: 2544 2574 224
Size: 30 2320
Fragments arranged by size:
2320 1554 473 165 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1709 1709
Size: 4670
KasI G'GCGC_C
Cuts at: 2100 2348 2100
Size: 248 4422
KpnI G_GTAC'C
Cuts at: 1690 1690
Size: 4670
MluI A'CGCG_T
Cuts at: 4103 4103
Size: 4670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3341 3525 3341
Size: 184 4486
MscI TGG'CCA
Cuts at: 2431 2431
Size: 4670
MslI CAynn'nnrTG
Cuts at: 206 1132 2659 2786 206
Size: 926 1527 127 2090
Fragments arranged by size:
2090 1527 926 127
MspA1I CmG'CkG
Cuts at: 1781 2095 2455 3468 3713 1781
Size: 314 360 1013 245 2738
Fragments arranged by size:
2738 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2851 2851
Size: 4670
NarI GG'CG_CC
Cuts at: 2101 2349 2101
Size: 248 4422
NgoMIV G'CCGG_C
Cuts at: 2849 2849
Size: 4670
NspI r_CATG'y
Cuts at: 1181 1723 2223 3130 1181
Size: 542 500 907 2721
Fragments arranged by size:
2721 907 542 500
PasI CC'CwG_GG
Cuts at: 1638 1638
Size: 4670
PciI A'CATG_T
Cuts at: 1177 3126 1177
Size: 1949 2721
PfoI T'CCnGG_A
Cuts at: 699 699
Size: 4670
PmeI GTTT'AAAC
Cuts at: 1669 1669
Size: 4670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 3809
Size: 32 4638
PshAI GACnn'nnGTC
Cuts at: 154 1438 154
Size: 1284 3386
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 518 550 518
Size: 32 4638
PstI C_TGCA'G
Cuts at: 1717 2402 1717
Size: 685 3985
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4670
PvuII CAG'CTG
Cuts at: 2095 2455 2095
Size: 360 4310
RsrII CG'GwC_CG
Cuts at: 2865 2865
Size: 4670
SacI G_AGCT'C
Cuts at: 1470 1684 1470
Size: 214 4456
SalI G'TCGA_C
Cuts at: 1707 1707
Size: 4670
SapI GCTCTTCn'nnn_
Cuts at: 2693 2903 2693
Size: 210 4460
SbfI CC_TGCA'GG
Cuts at: 1717 1717
Size: 4670
ScaI AGT'ACT
Cuts at: 4187 4187
Size: 4670
SfcI C'TryA_G
Cuts at: 33 952 1146 1713 2398 3391 3582 33
Size: 919 194 567 685 993 191 1121
Fragments arranged by size:
1121 993 919 685 567 194 191
SfoI GGC'GCC
Cuts at: 2102 2350 2102
Size: 248 4422
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
SmaI CCC'GGG
Cuts at: 1692 1692
Size: 4670
SmlI C'TyrA_G
Cuts at: 1211 1248 1825 2740 3232 3494 1211
Size: 37 577 915 492 262 2387
Fragments arranged by size:
2387 915 577 492 262 37
SnaBI TAC'GTA
Cuts at: 1338 1338
Size: 4670
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4670
SphI G_CATG'C
Cuts at: 1723 2223 1723
Size: 500 4170
StuI AGG'CCT
Cuts at: 923 923
Size: 4670
StyI C'CwwG_G
Cuts at: 387 1804 2158 387
Size: 1417 354 2899
Fragments arranged by size:
2899 1417 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 181 961 1328 1594 2253 2386 2845 3054
Size: 51 780 367 266 659 133 459 209
Cuts at: 3054 130
Size: 1746
Fragments arranged by size:
1746 780 659 459 367 266 209 133
51
TatI w'GTAC_w
Cuts at: 615 1614 4185 615
Size: 999 2571 1100
Fragments arranged by size:
2571 1100 999
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 756 788 857 889 4187 4219 756
Size: 32 69 32 3298 32 1207
Fragments arranged by size:
3298 1207 69 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 940 2467 940
Size: 1527 3143
XbaI T'CTAG_A
Cuts at: 1701 1701
Size: 4670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1635 2165 1635
Size: 530 4140
XmaI C'CCGG_G
Cuts at: 1690 1690
Size: 4670
XmnI GAAnn'nnTTC
Cuts at: 656 656
Size: 4670
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PshAI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PpuMI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SrfI SspI SwaI XhoI ZraI