Restriction map for
pF1KB9457
(Circular) (Six-base) MAPSORT of: pF1KB9457.seq Check: 3677 from: 1 to: 5609
pF1KB9457 5609 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AatII G_ACGT'C
Cuts at: 364 364
Size: 5609
AccI GT'mk_AC
Cuts at: 632 2548 2647 632
Size: 1916 99 3594
Fragments arranged by size:
3594 1916 99
Acc65I G'GTAC_C
Cuts at: 2625 2625
Size: 5609
AclI AA'CG_TT
Cuts at: 5449 5449
Size: 5609
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 746 3434 3866 4219 4881 394
Size: 352 2688 432 353 662 1122
Fragments arranged by size:
2688 1122 662 432 353 352
AflIII A'CryG_T
Cuts at: 1347 1534 1819 2961 4761 5042 1347
Size: 187 285 1142 1800 281 1914
Fragments arranged by size:
1914 1800 1142 285 281 187
AhdI GACnn_n'nnGTC
Cuts at: 655 655
Size: 5609
AleI CACnn'nnGTG
Cuts at: 1637 1637
Size: 5609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1655 2011 1655
Size: 356 5253
AlwNI CAG_nnn'CTG
Cuts at: 157 2914 4352 157
Size: 2757 1438 1414
Fragments arranged by size:
2757 1438 1414
ApaI G_GGCC'C
Cuts at: 946 946
Size: 5609
ApaLI G'TGCA_C
Cuts at: 4447 4447
Size: 5609
ApoI r'AATT_y
Cuts at: 142 163 186 1412 2613 4962 5218 142
Size: 21 23 1226 1201 2349 256 533
Fragments arranged by size:
2349 1226 1201 533 256 23 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 5609
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5609
AvaI C'yCGr_G
Cuts at: 1094 1718 2629 1094
Size: 624 911 4074
Fragments arranged by size:
4074 911 624
BamHI G'GATC_C
Cuts at: 1068 2634 1068
Size: 1566 4043
BanI G'GyrC_C
Cuts at: 117 1319 2625 3039 3287 3322 117
Size: 1202 1306 414 248 35 2404
Fragments arranged by size:
2404 1306 1202 414 248 35
BanII G_rGCy'C
Cuts at: 517 946 2623 3653 517
Size: 429 1677 1030 2473
Fragments arranged by size:
2473 1677 1030 429
BbeI G_GCGC'C
Cuts at: 121 3043 3291 121
Size: 2922 248 2439
Fragments arranged by size:
2922 2439 248
BbsI GAAGACnn'nnnn_
Cuts at: 1016 1016
Size: 5609
BbvCI CC'TCA_GC
Cuts at: 1841 1841
Size: 5609
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1546 1580 1546
Size: 34 5575
BciVI GTATCCnnnnn_n'
Cuts at: 3507 4563 5609 3507
Size: 1056 1046 3507
Fragments arranged by size:
3507 1056 1046
BclI T'GATC_A
Cuts at: 3129 3129
Size: 5609
BfrBI ATG'CAT
Cuts at: 1952 1952
Size: 5609
BglI GCCn_nnn'nGGC
Cuts at: 1895 1895
Size: 5609
BglII A'GATC_T
Cuts at: 3124 5161 3124
Size: 2037 3572
BlpI GC'TnA_GC
Cuts at: 482 1643 2721 482
Size: 1161 1078 3370
Fragments arranged by size:
3370 1161 1078
Bme1580I G_kGCm'C
Cuts at: 946 1324 3234 3327 4451 946
Size: 378 1910 93 1124 2104
Fragments arranged by size:
2104 1910 1124 378 93
BmrI ACTGGGnnnn_n'
Cuts at: 1210 2550 2821 2902 3237 5381 1210
Size: 1340 271 81 335 2144 1438
Fragments arranged by size:
2144 1438 1340 335 271 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 507 789 3976 507
Size: 282 3187 2140
Fragments arranged by size:
3187 2140 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 2363 1268
Size: 1095 4514
Bpu10I CC'TnA_GC
Cuts at: 1841 1841
Size: 5609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 1277 2785 3664 4378 4676 1058
Size: 219 1508 879 714 298 1991
Fragments arranged by size:
1991 1508 879 714 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 1286 5313 1286
Size: 4027 1582
BsaAI yAC'GTr
Cuts at: 2962 2962
Size: 5609
BsaBI GATnn'nnATC
Cuts at: 6 3123 5160 6
Size: 3117 2037 455
Fragments arranged by size:
3117 2037 455
BsaHI Gr'CG_yC
Cuts at: 118 361 2545 3040 3288 3990 5486 118
Size: 243 2184 495 248 702 1496 241
Fragments arranged by size:
2184 1496 702 495 248 243 241
BsaWI w'CCGG_w
Cuts at: 705 2798 3319 3997 4408 4555 4829 705
Size: 2093 521 678 411 147 274 1485
Fragments arranged by size:
2093 1485 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2221 2251 2221
Size: 30 5579
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1895 2488 1895
Size: 593 5016
BseYI C'CCAG_C
Cuts at: 1871 3034 3792 4457 4865 1871
Size: 1163 758 665 408 2615
Fragments arranged by size:
2615 1163 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 1328 2573 608
Size: 720 1245 3644
Fragments arranged by size:
3644 1245 720
BsiEI CG_ry'CG
Cuts at: 104 3151 3197 4427 104
Size: 3047 46 1230 1286
Fragments arranged by size:
3047 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 517 2234 2623 3401 3591 4451 517
Size: 1717 389 778 190 860 1675
Fragments arranged by size:
1717 1675 860 778 389 190
Bsp1286I G_dGCh'C
Cuts at: 517 946 1324 2234 2623 3234 3327 3401 3591
Size: 429 378 910 389 611 93 74 190
Cuts at: 3591 3653 4451 517
Size: 62 798 1675
Fragments arranged by size:
1675 910 798 611 429 389 378 190
93 74 62
BspHI T'CATG_A
Cuts at: 4041 4041
Size: 5609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 273 2356 2428 2595 2659 2873 3175 3556 273
Size: 2083 72 167 64 214 302 381 2326
Fragments arranged by size:
2326 2083 381 302 214 167 72 64
BsrBI CCG'CTC
Cuts at: 50 3901 3955 5439 5604 50
Size: 3851 54 1484 165 55
Fragments arranged by size:
3851 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1861 3521 1861
Size: 1660 3949
BsrFI r'CCGG_y
Cuts at: 3607 3788 3607
Size: 181 5428
BssSI C'ACGA_G
Cuts at: 3880 4588 3880
Size: 708 4901
BstAPI GCAn_nnn'nTGC
Cuts at: 1187 2986 1187
Size: 1799 3810
BstBI TT'CG_AA
Cuts at: 3970 3970
Size: 5609
BstEII G'GTnAC_C
Cuts at: 1810 1810
Size: 5609
BstXI CCAn_nnnn'nTGG
Cuts at: 738 738
Size: 5609
BstYI r'GATC_y
Cuts at: 1 591 1068 2634 3101 3124 3459 3705 4011
Size: 590 477 1566 467 23 335 246 306
Cuts at: 4011 4023 4109 5161 1
Size: 12 86 1052 449
Fragments arranged by size:
1566 1052 590 477 467 449 335 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 633 633
Size: 5609
Bsu36I CC'TnA_GG
Cuts at: 173 173
Size: 5609
BtgI C'CryG_G
Cuts at: 107 402 1653 1861 107
Size: 295 1251 208 3855
Fragments arranged by size:
3855 1251 295 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3559 3740 3903 3559
Size: 181 163 5265
Fragments arranged by size:
5265 181 163
BtsI GCAGTG_nn'
Cuts at: 2852 2852
Size: 5609
DraI TTT'AAA
Cuts at: 141 1781 2608 141
Size: 1640 827 3142
Fragments arranged by size:
3142 1640 827
DrdI GACnn_nn'nnGTC
Cuts at: 3315 4659 3315
Size: 1344 4265
EaeI y'GGCC_r
Cuts at: 1302 1455 1896 3194 3368 3759 3786 5131 1302
Size: 153 441 1298 174 391 27 1345 1780
Fragments arranged by size:
1780 1345 1298 441 391 174 153 27
EagI C'GGCC_G
Cuts at: 3194 3194
Size: 5609
EarI CTCTTCn'nnn_
Cuts at: 765 3632 3842 765
Size: 2867 210 2532
Fragments arranged by size:
2867 2532 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 3817 4559 4705 5419 127
Size: 3690 742 146 714 317
Fragments arranged by size:
3690 742 714 317 146
EcoICRI GAG'CTC
Cuts at: 515 2621 515
Size: 2106 3503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 746 1268 2363 3434 3866 4219 4881 394
Size: 352 522 1095 1071 432 353 662 1122
Fragments arranged by size:
1122 1095 1071 662 522 432 353 352
EcoNI CCTnn'n_nnAGG
Cuts at: 128 128
Size: 5609
EcoO109I rG'GnC_Cy
Cuts at: 285 943 1498 2399 2748 285
Size: 658 555 901 349 3146
Fragments arranged by size:
3146 901 658 555 349
EcoRI G'AATT_C
Cuts at: 163 2613 163
Size: 2450 3159
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3628 3628
Size: 5609
FspI TGC'GCA
Cuts at: 3390 3390
Size: 5609
HaeII r_GCGC'y
Cuts at: 121 3043 3291 4521 5292 121
Size: 2922 248 1230 771 438
Fragments arranged by size:
2922 1230 771 438 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 641 673 1053 1085 2361 2393 3451
Size: 32 323 32 380 32 1276 32 1058
Cuts at: 3451 3481 3483 3513 286
Size: 30 2 30 2382
Fragments arranged by size:
2382 1276 1058 380 323 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1038 2648 1038
Size: 1610 3999
KasI G'GCGC_C
Cuts at: 117 3039 3287 117
Size: 2922 248 2439
Fragments arranged by size:
2922 2439 248
KpnI G_GTAC'C
Cuts at: 2629 2629
Size: 5609
MfeI C'AATT_G
Cuts at: 1915 1915
Size: 5609
MluI A'CGCG_T
Cuts at: 5042 5042
Size: 5609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1294 1921 4368 4552 1294
Size: 627 2447 184 2351
Fragments arranged by size:
2447 2351 627 184
MscI TGG'CCA
Cuts at: 1304 1457 1898 3370 1304
Size: 153 441 1472 3543
Fragments arranged by size:
3543 1472 441 153
MslI CAynn'nnrTG
Cuts at: 1424 1539 1637 2027 2154 2282 2357 2438 2595
Size: 115 98 390 127 128 75 81 157
Cuts at: 2595 3598 3725 1424
Size: 1003 127 3308
Fragments arranged by size:
3308 1003 390 157 128 127 127 115
98 81 75
MspA1I CmG'CkG
Cuts at: 502 1775 1875 2720 3034 3394 4178 4423 502
Size: 1273 100 845 314 360 784 245 1688
Fragments arranged by size:
1688 1273 845 784 360 314 245 100
NaeI GCC'GGC
Cuts at: 3790 3790
Size: 5609
NarI GG'CG_CC
Cuts at: 118 3040 3288 118
Size: 2922 248 2439
Fragments arranged by size:
2922 2439 248
NcoI C'CATG_G
Cuts at: 107 402 1653 107
Size: 295 1251 4063
Fragments arranged by size:
4063 1251 295
NgoMIV G'CCGG_C
Cuts at: 3788 3788
Size: 5609
NsiI A_TGCA'T
Cuts at: 1954 1954
Size: 5609
NspI r_CATG'y
Cuts at: 1351 1538 1823 1952 2321 2600 2662 3162 4765
Size: 187 285 129 369 279 62 500 1603
Cuts at: 4765 1351
Size: 2195
Fragments arranged by size:
2195 1603 500 369 285 279 187 129
62
PasI CC'CwG_GG
Cuts at: 596 848 596
Size: 252 5357
PciI A'CATG_T
Cuts at: 1347 1534 1819 4761 1347
Size: 187 285 2942 2195
Fragments arranged by size:
2942 2195 285 187
PfoI T'CCnGG_A
Cuts at: 1027 1494 1027
Size: 467 5142
PmeI GTTT'AAAC
Cuts at: 2608 2608
Size: 5609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 4055
Size: 32 5577
PpuMI rG'GwC_Cy
Cuts at: 285 1498 285
Size: 1213 4396
PspOMI G'GGCC_C
Cuts at: 942 942
Size: 5609
PstI C_TGCA'G
Cuts at: 2092 2656 3341 2092
Size: 564 685 4360
Fragments arranged by size:
4360 685 564
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5609
PvuII CAG'CTG
Cuts at: 502 1775 1875 3034 3394 502
Size: 1273 100 1159 360 2717
Fragments arranged by size:
2717 1273 1159 360 100
RsrII CG'GwC_CG
Cuts at: 3804 3804
Size: 5609
SacI G_AGCT'C
Cuts at: 517 2623 517
Size: 2106 3503
SalI G'TCGA_C
Cuts at: 2646 2646
Size: 5609
SapI GCTCTTCn'nnn_
Cuts at: 3632 3842 3632
Size: 210 5399
SbfI CC_TGCA'GG
Cuts at: 2656 2656
Size: 5609
ScaI AGT'ACT
Cuts at: 5126 5126
Size: 5609
SexAI A'CCwGG_T
Cuts at: 1691 1691
Size: 5609
SfcI C'TryA_G
Cuts at: 33 2088 2652 3337 4305 4496 33
Size: 2055 564 685 968 191 1146
Fragments arranged by size:
2055 1146 968 685 564 191
SfoI GGC'GCC
Cuts at: 119 3041 3289 119
Size: 2922 248 2439
Fragments arranged by size:
2922 2439 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5609
SmaI CCC'GGG
Cuts at: 2631 2631
Size: 5609
SmlI C'TyrA_G
Cuts at: 1073 1292 2764 3679 4393 4655 1073
Size: 219 1472 915 714 262 2027
Fragments arranged by size:
2027 1472 915 714 262 219
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5609
SphI G_CATG'C
Cuts at: 2662 3162 2662
Size: 500 5109
SspI AAT'ATT
Cuts at: 1614 1614
Size: 5609
StuI AGG'CCT
Cuts at: 843 843
Size: 5609
StyI C'CwwG_G
Cuts at: 107 402 421 1653 2743 3097 107
Size: 295 19 1232 1090 354 2619
Fragments arranged by size:
2619 1232 1090 354 295 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 574 3192 3325 3784 3993 574
Size: 2618 133 459 209 2190
Fragments arranged by size:
2618 2190 459 209 133
TatI w'GTAC_w
Cuts at: 5124 5124
Size: 5609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2221 2253 5126 5158 2221
Size: 32 2873 32 2672
Fragments arranged by size:
2873 2672 32 32
Tth111I GACn'n_nGTC
Cuts at: 3406 3406
Size: 5609
XbaI T'CTAG_A
Cuts at: 775 2640 775
Size: 1865 3744
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3104 3104
Size: 5609
XmaI C'CCGG_G
Cuts at: 2629 2629
Size: 5609
XmnI GAAnn'nnTTC
Cuts at: 167 1199 167
Size: 1032 4577
ZraI GAC'GTC
Cuts at: 362 362
Size: 5609
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AloI AscI AvrII BaeI
BmgBI BmtI BsiWI BsmI BsmBI BspEI BsrGI BssHII
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI PacI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI