Restriction map for
pF1KB9460
(Circular) (Six-base) MAPSORT of: pF1KB9460.seq Check: 7510 from: 1 to: 5297
pF1KB9460 5297 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 2335 2335
Size: 5297
Acc65I G'GTAC_C
Cuts at: 2313 2313
Size: 5297
AclI AA'CG_TT
Cuts at: 5137 5137
Size: 5297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 2194 3122 3554 3907 4569 955
Size: 1239 928 432 353 662 1683
Fragments arranged by size:
1683 1239 928 662 432 353
AfeI AGC'GCT
Cuts at: 253 1010 1630 253
Size: 757 620 3920
Fragments arranged by size:
3920 757 620
AflII C'TTAA_G
Cuts at: 1444 1444
Size: 5297
AflIII A'CryG_T
Cuts at: 2649 4449 4730 2649
Size: 1800 281 3216
Fragments arranged by size:
3216 1800 281
AleI CACnn'nnGTG
Cuts at: 328 328
Size: 5297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1013 1013
Size: 5297
AlwNI CAG_nnn'CTG
Cuts at: 228 792 1527 1743 2602 4040 228
Size: 564 735 216 859 1438 1485
Fragments arranged by size:
1485 1438 859 735 564 216
ApaI G_GGCC'C
Cuts at: 118 347 385 420 699 719 843 1752 2269
Size: 229 38 35 279 20 124 909 517
Cuts at: 2269 118
Size: 3146
Fragments arranged by size:
3146 909 517 279 229 124 38 35
20
ApaLI G'TGCA_C
Cuts at: 4135 4135
Size: 5297
ApoI r'AATT_y
Cuts at: 2301 4650 4906 2301
Size: 2349 256 2692
Fragments arranged by size:
2692 2349 256
AscI GG'CGCG_CC
Cuts at: 688 688
Size: 5297
AseI AT'TA_AT
Cuts at: 20 20
Size: 5297
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5297
AvaI C'yCGr_G
Cuts at: 451 670 2262 2317 451
Size: 219 1592 55 3431
Fragments arranged by size:
3431 1592 219 55
BamHI G'GATC_C
Cuts at: 1667 2322 1667
Size: 655 4642
BanI G'GyrC_C
Cuts at: 322 847 1156 1435 1861 2032 2313 2727 2975
Size: 525 309 279 426 171 281 414 248
Cuts at: 2975 3010 322
Size: 35 2609
Fragments arranged by size:
2609 525 426 414 309 281 279 248
171 35
BanII G_rGCy'C
Cuts at: 118 347 385 420 699 719 843 932 1072
Size: 229 38 35 279 20 124 89 140
Cuts at: 1072 1752 2153 2269 2311 3341 118
Size: 680 401 116 42 1030 2074
Fragments arranged by size:
2074 1030 680 401 279 229 140 124
116 89 42 38 35 20
BbeI G_GCGC'C
Cuts at: 851 1439 1865 2731 2979 851
Size: 588 426 866 248 3169
Fragments arranged by size:
3169 866 588 426 248
BbsI GAAGACnn'nnnn_
Cuts at: 1239 1239
Size: 5297
BciVI GTATCCnnnnn_n'
Cuts at: 3195 4251 5297 3195
Size: 1056 1046 3195
Fragments arranged by size:
3195 1056 1046
BclI T'GATC_A
Cuts at: 1238 2817 1238
Size: 1579 3718
BglI GCCn_nnn'nGGC
Cuts at: 122 223 380 402 723 2063 122
Size: 101 157 22 321 1340 3356
Fragments arranged by size:
3356 1340 321 157 101 22
BglII A'GATC_T
Cuts at: 1574 2812 4849 1574
Size: 1238 2037 2022
Fragments arranged by size:
2037 2022 1238
BlpI GC'TnA_GC
Cuts at: 2014 2409 2014
Size: 395 4902
Bme1580I G_kGCm'C
Cuts at: 118 347 385 420 699 719 843 1265 1752
Size: 229 38 35 279 20 124 422 487
Cuts at: 1752 2269 2922 3015 4139 118
Size: 517 653 93 1124 1276
Fragments arranged by size:
1276 1124 653 517 487 422 279 229
124 93 38 35 20
BmgBI CAC'GTC
Cuts at: 195 591 195
Size: 396 4901
BmrI ACTGGGnnnn_n'
Cuts at: 1110 1335 2245 2509 2590 2925 5069 1110
Size: 225 910 264 81 335 2144 1338
Fragments arranged by size:
2144 1338 910 335 264 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 477 3664 477
Size: 3187 2110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 754 769 1645 1753 196
Size: 558 15 876 108 3740
Fragments arranged by size:
3740 876 558 108 15
Bpu10I CC'TnA_GC
Cuts at: 422 583 422
Size: 161 5136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1583 1675 1780 2473 3352 4066 4364 1583
Size: 92 105 693 879 714 298 2516
Fragments arranged by size:
2516 879 714 693 298 105 92
BsaI GGTCTCn'nnnn_
Cuts at: 1823 5001 1823
Size: 3178 2119
BsaAI yAC'GTr
Cuts at: 1977 2650 1977
Size: 673 4624
BsaBI GATnn'nnATC
Cuts at: 6 2811 4848 6
Size: 2805 2037 455
Fragments arranged by size:
2805 2037 455
BsaHI Gr'CG_yC
Cuts at: 848 1436 1862 2728 2976 3678 5174 848
Size: 588 426 866 248 702 1496 971
Fragments arranged by size:
1496 971 866 702 588 426 248
BsaWI w'CCGG_w
Cuts at: 2486 3007 3685 4096 4243 4517 2486
Size: 521 678 411 147 274 3266
Fragments arranged by size:
3266 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2062 2092 2062
Size: 30 5267
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1135 1360 2020 1135
Size: 225 660 4412
Fragments arranged by size:
4412 660 225
BseYI C'CCAG_C
Cuts at: 228 931 1005 1439 1890 2722 3480 4145 4553
Size: 703 74 434 451 832 758 665 408
Cuts at: 4553 228
Size: 972
Fragments arranged by size:
972 832 758 703 665 451 434 408
74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 2173 2233 739
Size: 1434 60 3803
Fragments arranged by size:
3803 1434 60
BsiEI CG_ry'CG
Cuts at: 104 166 683 2839 2885 4115 104
Size: 62 517 2156 46 1230 1286
Fragments arranged by size:
2156 1286 1230 517 62 46
BsiHKAI G_wGCw'C
Cuts at: 2311 3089 3279 4139 2311
Size: 778 190 860 3469
Fragments arranged by size:
3469 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 118 347 385 420 699 719 843 932 1072
Size: 229 38 35 279 20 124 89 140
Cuts at: 1072 1265 1752 2153 2269 2311 2922 3015 3089
Size: 193 487 401 116 42 611 93 74
Cuts at: 3089 3279 3341 4139 118
Size: 190 62 798 1276
Fragments arranged by size:
1276 798 611 487 401 279 229 193
190 140 124 116 93 89 74 62
42 38 35 20
BspHI T'CATG_A
Cuts at: 3729 3729
Size: 5297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2347 2561 2863 3244 2347
Size: 214 302 381 4400
Fragments arranged by size:
4400 381 302 214
BsrBI CCG'CTC
Cuts at: 50 162 660 1686 2018 3589 3643 5127 5292
Size: 112 498 1026 332 1571 54 1484 165
Cuts at: 5292 50
Size: 55
Fragments arranged by size:
1571 1484 1026 498 332 165 112 55
54
BsrDI GCAATG_nn'
Cuts at: 1700 3209 1700
Size: 1509 3788
BsrFI r'CCGG_y
Cuts at: 726 1038 2131 3295 3476 726
Size: 312 1093 1164 181 2547
Fragments arranged by size:
2547 1164 1093 312 181
BssHII G'CGCG_C
Cuts at: 688 761 873 875 2256 688
Size: 73 112 2 1381 3729
Fragments arranged by size:
3729 1381 112 73 2
BssSI C'ACGA_G
Cuts at: 661 1327 1840 1957 3568 4276 661
Size: 666 513 117 1611 708 1682
Fragments arranged by size:
1682 1611 708 666 513 117
BstAPI GCAn_nnn'nTGC
Cuts at: 1743 2253 2674 1743
Size: 510 421 4366
Fragments arranged by size:
4366 510 421
BstBI TT'CG_AA
Cuts at: 3658 3658
Size: 5297
BstXI CCAn_nnnn'nTGG
Cuts at: 1053 1258 1053
Size: 205 5092
BstYI r'GATC_y
Cuts at: 1 1187 1574 1667 1709 1823 2322 2789 2812
Size: 1186 387 93 42 114 499 467 23
Cuts at: 2812 3147 3393 3699 3711 3797 4849 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1186 1052 499 467 449 387 335 306
246 114 93 86 42 23 12
Bsu36I CC'TnA_GG
Cuts at: 119 1213 119
Size: 1094 4203
BtgI C'CryG_G
Cuts at: 107 347 376 710 719 830 2081 107
Size: 240 29 334 9 111 1251 3323
Fragments arranged by size:
3323 1251 334 240 111 29 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3247 3428 3591 3247
Size: 181 163 4953
Fragments arranged by size:
4953 181 163
BtsI GCAGTG_nn'
Cuts at: 391 646 1436 2106 2540 391
Size: 255 790 670 434 3148
Fragments arranged by size:
3148 790 670 434 255
DraI TTT'AAA
Cuts at: 2296 2296
Size: 5297
DraIII CAC_nnn'GTG
Cuts at: 2253 2253
Size: 5297
DrdI GACnn_nn'nnGTC
Cuts at: 1405 3003 4347 1405
Size: 1598 1344 2355
Fragments arranged by size:
2355 1598 1344
EaeI y'GGCC_r
Cuts at: 163 236 680 1055 2126 2882 3056 3447 3474
Size: 73 444 375 1071 756 174 391 27
Cuts at: 3474 4819 163
Size: 1345 641
Fragments arranged by size:
1345 1071 756 641 444 391 375 174
73 27
EagI C'GGCC_G
Cuts at: 163 680 2882 163
Size: 517 2202 2578
Fragments arranged by size:
2578 2202 517
EarI CTCTTCn'nnn_
Cuts at: 491 980 1496 1577 3320 3530 491
Size: 489 516 81 1743 210 2258
Fragments arranged by size:
2258 1743 516 489 210 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 142 3505 4247 4393 5107 142
Size: 3363 742 146 714 332
Fragments arranged by size:
3363 742 714 332 146
EcoICRI GAG'CTC
Cuts at: 2309 2309
Size: 5297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 754 769 955 1645 1753 2194 3122 3554
Size: 558 15 186 690 108 441 928 432
Cuts at: 3554 3907 4569 196
Size: 353 662 924
Fragments arranged by size:
928 924 690 662 558 441 432 353
186 108 15
EcoNI CCTnn'n_nnAGG
Cuts at: 425 703 425
Size: 278 5019
EcoO109I rG'GnC_Cy
Cuts at: 115 171 343 381 382 404 416 417 696
Size: 56 172 38 1 22 12 1 279
Cuts at: 696 715 826 840 2266 2436 115
Size: 19 111 14 1426 170 2976
Fragments arranged by size:
2976 1426 279 172 170 111 56 38
22 19 14 12 1 1
EcoRI G'AATT_C
Cuts at: 2301 2301
Size: 5297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3316 3316
Size: 5297
FspI TGC'GCA
Cuts at: 1819 3078 1819
Size: 1259 4038
HaeII r_GCGC'y
Cuts at: 229 255 733 851 914 952 1012 1439 1632
Size: 26 478 118 63 38 60 427 193
Cuts at: 1632 1865 2731 2979 4209 4980 229
Size: 233 866 248 1230 771 546
Fragments arranged by size:
1230 866 771 546 478 427 248 233
193 118 63 60 38 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 520 552 3139 3169 3171 3201 520
Size: 32 2587 30 2 30 2616
Fragments arranged by size:
2616 2587 32 30 30 2
HincII GTy'rAC
Cuts at: 2336 2336
Size: 5297
HindIII A'AGCT_T
Cuts at: 496 496
Size: 5297
KasI G'GCGC_C
Cuts at: 847 1435 1861 2727 2975 847
Size: 588 426 866 248 3169
Fragments arranged by size:
3169 866 588 426 248
KpnI G_GTAC'C
Cuts at: 2317 2317
Size: 5297
MfeI C'AATT_G
Cuts at: 1547 1547
Size: 5297
MluI A'CGCG_T
Cuts at: 4730 4730
Size: 5297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4056 4240 4056
Size: 184 5113
MscI TGG'CCA
Cuts at: 1057 3058 1057
Size: 2001 3296
MslI CAynn'nnrTG
Cuts at: 328 1256 3286 3413 328
Size: 928 2030 127 2212
Fragments arranged by size:
2212 2030 928 127
MspA1I CmG'CkG
Cuts at: 712 753 832 864 1743 1815 2061 2408 2722
Size: 41 79 32 879 72 246 347 314
Cuts at: 2722 3082 3866 4111 712
Size: 360 784 245 1898
Fragments arranged by size:
1898 879 784 360 347 314 246 245
79 72 41 32
NaeI GCC'GGC
Cuts at: 728 1040 3478 728
Size: 312 2438 2547
Fragments arranged by size:
2547 2438 312
NarI GG'CG_CC
Cuts at: 848 1436 1862 2728 2976 848
Size: 588 426 866 248 3169
Fragments arranged by size:
3169 866 588 426 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5297
NgoMIV G'CCGG_C
Cuts at: 726 1038 3476 726
Size: 312 2438 2547
Fragments arranged by size:
2547 2438 312
NspI r_CATG'y
Cuts at: 2350 2850 4453 2350
Size: 500 1603 3194
Fragments arranged by size:
3194 1603 500
PasI CC'CwG_GG
Cuts at: 822 843 822
Size: 21 5276
PciI A'CATG_T
Cuts at: 4449 4449
Size: 5297
PflMI CCAn_nnn'nTGG
Cuts at: 792 792
Size: 5297
PfoI T'CCnGG_A
Cuts at: 1139 1139
Size: 5297
PmeI GTTT'AAAC
Cuts at: 2296 2296
Size: 5297
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 5265
PpuMI rG'GwC_Cy
Cuts at: 171 826 171
Size: 655 4642
PspOMI G'GGCC_C
Cuts at: 114 343 381 416 695 715 839 1748 2265
Size: 229 38 35 279 20 124 909 517
Cuts at: 2265 114
Size: 3146
Fragments arranged by size:
3146 909 517 279 229 124 38 35
20
PstI C_TGCA'G
Cuts at: 1085 1532 2344 3029 1085
Size: 447 812 685 3353
Fragments arranged by size:
3353 812 685 447
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5297
PvuII CAG'CTG
Cuts at: 1743 1815 2722 3082 1743
Size: 72 907 360 3958
Fragments arranged by size:
3958 907 360 72
RsrII CG'GwC_CG
Cuts at: 834 3492 834
Size: 2658 2639
SacI G_AGCT'C
Cuts at: 2311 2311
Size: 5297
SacII CC_GC'GG
Cuts at: 713 833 713
Size: 120 5177
SalI G'TCGA_C
Cuts at: 2334 2334
Size: 5297
SanDI GG'GwC_CC
Cuts at: 826 826
Size: 5297
SapI GCTCTTCn'nnn_
Cuts at: 980 1577 3320 3530 980
Size: 597 1743 210 2747
Fragments arranged by size:
2747 1743 597 210
SbfI CC_TGCA'GG
Cuts at: 1085 2344 1085
Size: 1259 4038
ScaI AGT'ACT
Cuts at: 4814 4814
Size: 5297
SfcI C'TryA_G
Cuts at: 33 1081 1528 2340 3025 3993 4184 33
Size: 1048 447 812 685 968 191 1146
Fragments arranged by size:
1146 1048 968 812 685 447 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 723 723
Size: 5297
SfoI GGC'GCC
Cuts at: 849 1437 1863 2729 2977 849
Size: 588 426 866 248 3169
Fragments arranged by size:
3169 866 588 426 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5297
SmaI CCC'GGG
Cuts at: 2264 2319 2264
Size: 55 5242
SmlI C'TyrA_G
Cuts at: 1444 1598 1690 1795 2452 3367 4081 4343 1444
Size: 154 92 105 657 915 714 262 2398
Fragments arranged by size:
2398 915 714 657 262 154 105 92
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5297
SphI G_CATG'C
Cuts at: 2350 2850 2350
Size: 500 4797
StuI AGG'CCT
Cuts at: 431 676 1555 1756 431
Size: 245 879 201 3972
Fragments arranged by size:
3972 879 245 201
StyI C'CwwG_G
Cuts at: 107 1028 2431 2785 107
Size: 921 1403 354 2619
Fragments arranged by size:
2619 1403 921 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1867 2264 2880 3013 3472 3681 1867
Size: 397 616 133 459 209 3483
Fragments arranged by size:
3483 616 459 397 209 133
TatI w'GTAC_w
Cuts at: 1199 1343 1505 4812 1199
Size: 144 162 3307 1684
Fragments arranged by size:
3307 1684 162 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1078 1110 4814 4846 1078
Size: 32 3704 32 1529
Fragments arranged by size:
3704 1529 32 32
Tth111I GACn'n_nGTC
Cuts at: 3094 3094
Size: 5297
XbaI T'CTAG_A
Cuts at: 2328 2328
Size: 5297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2792 2792
Size: 5297
XmaI C'CCGG_G
Cuts at: 2262 2317 2262
Size: 55 5242
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AgeI AhdI AloI AvrII BaeI BbvCI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BspEI BsrGI
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI