Restriction map for
pF1KB9462
(Circular) (Six-base) MAPSORT of: pF1KB9462.seq Check: 5674 from: 1 to: 5849
pF1KB9462 5849 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 439 1610 2068 439
Size: 1171 458 4220
Fragments arranged by size:
4220 1171 458
AccI GT'mk_AC
Cuts at: 2633 2887 2633
Size: 254 5595
Acc65I G'GTAC_C
Cuts at: 1748 2865 1748
Size: 1117 4732
AclI AA'CG_TT
Cuts at: 5689 5689
Size: 5849
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 2202 3674 4106 4459 5121 560
Size: 1642 1472 432 353 662 1288
Fragments arranged by size:
1642 1472 1288 662 432 353
AfeI AGC'GCT
Cuts at: 2164 2798 2164
Size: 634 5215
AflII C'TTAA_G
Cuts at: 316 1447 316
Size: 1131 4718
AflIII A'CryG_T
Cuts at: 552 713 1783 3201 5001 5282 552
Size: 161 1070 1418 1800 281 1119
Fragments arranged by size:
1800 1418 1119 1070 281 161
AleI CACnn'nnGTG
Cuts at: 1933 1933
Size: 5849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1459 1459
Size: 5849
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 754 786 754
Size: 32 5817
AlwNI CAG_nnn'CTG
Cuts at: 465 1650 2241 3154 4592 465
Size: 1185 591 913 1438 1722
Fragments arranged by size:
1722 1438 1185 913 591
ApaI G_GGCC'C
Cuts at: 144 144
Size: 5849
ApaLI G'TGCA_C
Cuts at: 1269 2406 4687 1269
Size: 1137 2281 2431
Fragments arranged by size:
2431 2281 1137
ApoI r'AATT_y
Cuts at: 228 2853 5202 5458 228
Size: 2625 2349 256 619
Fragments arranged by size:
2625 2349 619 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5849
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5849
AvaI C'yCGr_G
Cuts at: 454 1968 2440 2869 454
Size: 1514 472 429 3434
Fragments arranged by size:
3434 1514 472 429
BamHI G'GATC_C
Cuts at: 2874 2874
Size: 5849
BanI G'GyrC_C
Cuts at: 412 702 1435 1748 2056 2527 2865 3279 3527
Size: 290 733 313 308 471 338 414 248
Cuts at: 3527 3562 412
Size: 35 2699
Fragments arranged by size:
2699 733 471 414 338 313 308 290
248 35
BanII G_rGCy'C
Cuts at: 144 292 586 887 1039 1054 1409 1861 2048
Size: 148 294 301 152 15 355 452 187
Cuts at: 2048 2774 2863 3893 144
Size: 726 89 1030 2100
Fragments arranged by size:
2100 1030 726 452 355 301 294 187
152 148 89 15
BbeI G_GCGC'C
Cuts at: 416 3283 3531 416
Size: 2867 248 2734
Fragments arranged by size:
2867 2734 248
BbsI GAAGACnn'nnnn_
Cuts at: 1705 1705
Size: 5849
BbvCI CC'TCA_GC
Cuts at: 166 1091 166
Size: 925 4924
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5815
BciVI GTATCCnnnnn_n'
Cuts at: 3747 4803 5849 3747
Size: 1056 1046 3747
Fragments arranged by size:
3747 1056 1046
BclI T'GATC_A
Cuts at: 1925 3369 1925
Size: 1444 4405
BglI GCCn_nnn'nGGC
Cuts at: 176 2378 176
Size: 2202 3647
BglII A'GATC_T
Cuts at: 3364 5401 3364
Size: 2037 3812
BlpI GC'TnA_GC
Cuts at: 1228 2042 2961 1228
Size: 814 919 4116
Fragments arranged by size:
4116 919 814
Bme1580I G_kGCm'C
Cuts at: 144 707 753 1273 2178 2410 2441 2669 3474
Size: 563 46 520 905 232 31 228 805
Cuts at: 3474 3567 4691 144
Size: 93 1124 1302
Fragments arranged by size:
1302 1124 905 805 563 520 232 228
93 46 31
BmgBI CAC'GTC
Cuts at: 1260 1784 1260
Size: 524 5325
BmrI ACTGGGnnnn_n'
Cuts at: 268 855 3061 3142 3477 5621 268
Size: 587 2206 81 335 2144 496
Fragments arranged by size:
2206 2144 587 496 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1875 4216 1875
Size: 2341 3508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 420 946 1009 1099 1137 364
Size: 56 526 63 90 38 5076
Fragments arranged by size:
5076 526 90 63 56 38
Bpu10I CC'TnA_GC
Cuts at: 166 1091 166
Size: 925 4924
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1228 1870 2323 2806 3025 3904 4618 4916
Size: 489 642 453 483 219 879 714 298
Cuts at: 4916 739
Size: 1672
Fragments arranged by size:
1672 879 714 642 489 483 453 298
219
BsaI GGTCTCn'nnnn_
Cuts at: 594 2437 2583 5553 594
Size: 1843 146 2970 890
Fragments arranged by size:
2970 1843 890 146
BsaAI yAC'GTr
Cuts at: 716 1989 2405 3202 716
Size: 1273 416 797 3363
Fragments arranged by size:
3363 1273 797 416
BsaBI GATnn'nnATC
Cuts at: 6 2098 3363 5400 6
Size: 2092 1265 2037 455
Fragments arranged by size:
2092 2037 1265 455
BsaHI Gr'CG_yC
Cuts at: 413 3280 3528 4230 5726 413
Size: 2867 248 702 1496 536
Fragments arranged by size:
2867 1496 702 536 248
BsaWI w'CCGG_w
Cuts at: 3038 3559 4237 4648 4795 5069 3038
Size: 521 678 411 147 274 3818
Fragments arranged by size:
3818 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 676 706 418
Size: 30 228 30 5561
Fragments arranged by size:
5561 228 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 1096 154
Size: 942 4907
BseYI C'CCAG_C
Cuts at: 408 1046 1439 2241 2376 2793 3274 4032 4697
Size: 638 393 802 135 417 481 758 665
Cuts at: 4697 5105 408
Size: 408 1152
Fragments arranged by size:
1152 802 758 665 638 481 417 408
393 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 1057 1345 1351 1588 496
Size: 561 288 6 237 4757
Fragments arranged by size:
4757 561 288 237 6
BsiEI CG_ry'CG
Cuts at: 104 1143 1398 2543 3391 3437 4667 104
Size: 1039 255 1145 848 46 1230 1286
Fragments arranged by size:
1286 1230 1145 1039 848 255 46
BsiHKAI G_wGCw'C
Cuts at: 586 1273 2339 2410 2774 2863 3641 3831 4691
Size: 687 1066 71 364 89 778 190 860
Cuts at: 4691 586
Size: 1744
Fragments arranged by size:
1744 1066 860 778 687 364 190 89
71
BsiWI C'GTAC_G
Cuts at: 1128 1128
Size: 5849
BsmI GAATG_Cn'
Cuts at: 419 2618 419
Size: 2199 3650
Bsp1286I G_dGCh'C
Cuts at: 144 292 586 707 753 887 1039 1054 1273
Size: 148 294 121 46 134 152 15 219
Cuts at: 1273 1409 1861 2048 2178 2339 2410 2441 2669
Size: 136 452 187 130 161 71 31 228
Cuts at: 2669 2774 2863 3474 3567 3641 3831 3893 4691
Size: 105 89 611 93 74 190 62 798
Cuts at: 4691 144
Size: 1302
Fragments arranged by size:
1302 798 611 452 294 228 219 190
187 161 152 148 136 134 130 121
105 93 89 74 71 62 46 31
15
BspHI T'CATG_A
Cuts at: 2033 4281 2033
Size: 2248 3601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 398 439 1610 2068 2899 3113 3415 3796 398
Size: 41 1171 458 831 214 302 381 2451
Fragments arranged by size:
2451 1171 831 458 381 302 214 41
BsrBI CCG'CTC
Cuts at: 50 806 1620 1640 1658 1692 4141 4195 5679
Size: 756 814 20 18 34 2449 54 1484
Cuts at: 5679 5844 50
Size: 165 55
Fragments arranged by size:
2449 1484 814 756 165 55 54 34
20 18
BsrDI GCAATG_nn'
Cuts at: 3761 3761
Size: 5849
BsrFI r'CCGG_y
Cuts at: 338 1175 1826 3847 4028 338
Size: 837 651 2021 181 2159
Fragments arranged by size:
2159 2021 837 651 181
BsrGI T'GTAC_A
Cuts at: 2480 2552 2480
Size: 72 5777
BssSI C'ACGA_G
Cuts at: 4120 4828 4120
Size: 708 5141
BstAPI GCAn_nnn'nTGC
Cuts at: 1374 1650 1718 1823 2664 3226 1374
Size: 276 68 105 841 562 3997
Fragments arranged by size:
3997 841 562 276 105 68
BstBI TT'CG_AA
Cuts at: 934 4210 934
Size: 3276 2573
BstXI CCAn_nnnn'nTGG
Cuts at: 600 600
Size: 5849
BstYI r'GATC_y
Cuts at: 1 2874 3341 3364 3699 3945 4251 4263 4349
Size: 2873 467 23 335 246 306 12 86
Cuts at: 4349 5401 1
Size: 1052 449
Fragments arranged by size:
2873 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 731 877 1352 1892 731
Size: 146 475 540 4688
Fragments arranged by size:
4688 540 475 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 840 1464 1887 3799 3980 4143 840
Size: 624 423 1912 181 163 2546
Fragments arranged by size:
2546 1912 624 423 181 163
BtsI GCAGTG_nn'
Cuts at: 525 1218 1728 3092 525
Size: 693 510 1364 3282
Fragments arranged by size:
3282 1364 693 510
ClaI AT'CG_AT
Cuts at: 1337 1337
Size: 5849
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 288 323 288
Size: 35 5814
DraI TTT'AAA
Cuts at: 2848 2848
Size: 5849
DraIII CAC_nnn'GTG
Cuts at: 2283 2283
Size: 5849
DrdI GACnn_nn'nnGTC
Cuts at: 3555 4899 3555
Size: 1344 4505
EaeI y'GGCC_r
Cuts at: 282 602 1395 1630 1889 2207 2540 2705 2801
Size: 320 793 235 259 318 333 165 96
Cuts at: 2801 3434 3608 3999 4026 5371 282
Size: 633 174 391 27 1345 760
Fragments arranged by size:
1345 793 760 633 391 333 320 318
259 235 174 165 96 27
EagI C'GGCC_G
Cuts at: 1395 2540 3434 1395
Size: 1145 894 3810
Fragments arranged by size:
3810 1145 894
EarI CTCTTCn'nnn_
Cuts at: 112 625 1888 2650 3872 4082 112
Size: 513 1263 762 1222 210 1879
Fragments arranged by size:
1879 1263 1222 762 513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 904 4057 4799 4945 5659 904
Size: 3153 742 146 714 1094
Fragments arranged by size:
3153 1094 742 714 146
EcoICRI GAG'CTC
Cuts at: 584 2772 2861 584
Size: 2188 89 3572
Fragments arranged by size:
3572 2188 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 420 560 946 1009 1099 1137 2202 3674
Size: 56 140 386 63 90 38 1065 1472
Cuts at: 3674 4106 4459 5121 364
Size: 432 353 662 1092
Fragments arranged by size:
1472 1092 1065 662 432 386 353 140
90 63 56 38
EcoNI CCTnn'n_nnAGG
Cuts at: 435 1081 1246 1742 435
Size: 646 165 496 4542
Fragments arranged by size:
4542 646 496 165
EcoO109I rG'GnC_Cy
Cuts at: 2790 2988 2790
Size: 198 5651
EcoRI G'AATT_C
Cuts at: 228 2853 228
Size: 2625 3224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 141 2757 3868 141
Size: 2616 1111 2122
Fragments arranged by size:
2616 2122 1111
FspI TGC'GCA
Cuts at: 1555 3630 1555
Size: 2075 3774
HaeII r_GCGC'y
Cuts at: 416 484 658 1530 2166 2597 2800 3283 3531
Size: 68 174 872 636 431 203 483 248
Cuts at: 3531 4761 5532 416
Size: 1230 771 733
Fragments arranged by size:
1230 872 771 733 636 483 431 248
203 174 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 676 708 2749 2781 3691 3721 3723
Size: 32 226 32 2041 32 910 30 2
Cuts at: 3723 3753 418
Size: 30 2514
Fragments arranged by size:
2514 2041 910 226 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1467 2888 1467
Size: 1421 4428
HindIII A'AGCT_T
Cuts at: 1846 1846
Size: 5849
KasI G'GCGC_C
Cuts at: 412 3279 3527 412
Size: 2867 248 2734
Fragments arranged by size:
2867 2734 248
KpnI G_GTAC'C
Cuts at: 1752 2869 1752
Size: 1117 4732
MluI A'CGCG_T
Cuts at: 5282 5282
Size: 5849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2741 4608 4792 2741
Size: 1867 184 3798
Fragments arranged by size:
3798 1867 184
MscI TGG'CCA
Cuts at: 604 2209 2803 3610 604
Size: 1605 594 807 2843
Fragments arranged by size:
2843 1605 807 594
MslI CAynn'nnrTG
Cuts at: 598 1933 2251 3838 3965 598
Size: 1335 318 1587 127 2482
Fragments arranged by size:
2482 1587 1335 318 127
MspA1I CmG'CkG
Cuts at: 512 1046 1443 1551 1650 2205 2238 2304 2960
Size: 534 397 108 99 555 33 66 656
Cuts at: 2960 3274 3634 4418 4663 512
Size: 314 360 784 245 1698
Fragments arranged by size:
1698 784 656 555 534 397 360 314
245 108 99 66 33
NaeI GCC'GGC
Cuts at: 1828 4030 1828
Size: 2202 3647
NarI GG'CG_CC
Cuts at: 413 3280 3528 413
Size: 2867 248 2734
Fragments arranged by size:
2867 2734 248
NcoI C'CATG_G
Cuts at: 731 1352 731
Size: 621 5228
NgoMIV G'CCGG_C
Cuts at: 1826 4028 1826
Size: 2202 3647
NspI r_CATG'y
Cuts at: 1716 1800 2391 2625 2902 3402 5005 1716
Size: 84 591 234 277 500 1603 2560
Fragments arranged by size:
2560 1603 591 500 277 234 84
PasI CC'CwG_GG
Cuts at: 198 1865 2607 198
Size: 1667 742 3440
Fragments arranged by size:
3440 1667 742
PciI A'CATG_T
Cuts at: 5001 5001
Size: 5849
PflMI CCAn_nnn'nTGG
Cuts at: 342 732 2283 342
Size: 390 1551 3908
Fragments arranged by size:
3908 1551 390
PfoI T'CCnGG_A
Cuts at: 923 1588 2294 923
Size: 665 706 4478
Fragments arranged by size:
4478 706 665
PmeI GTTT'AAAC
Cuts at: 2848 2848
Size: 5849
PmlI CAC'GTG
Cuts at: 2405 2405
Size: 5849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 4295
Size: 32 5817
PshAI GACnn'nnGTC
Cuts at: 1388 2818 1388
Size: 1430 4419
PspOMI G'GGCC_C
Cuts at: 140 140
Size: 5849
PspXI vC'TCGA_Gb
Cuts at: 454 454
Size: 5849
PstI C_TGCA'G
Cuts at: 1669 2237 2896 3581 1669
Size: 568 659 685 3937
Fragments arranged by size:
3937 685 659 568
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5849
PvuII CAG'CTG
Cuts at: 1046 1443 1551 1650 2205 2238 3274 3634 1046
Size: 397 108 99 555 33 1036 360 3261
Fragments arranged by size:
3261 1036 555 397 360 108 99 33
RsrII CG'GwC_CG
Cuts at: 4044 4044
Size: 5849
SacI G_AGCT'C
Cuts at: 586 2774 2863 586
Size: 2188 89 3572
Fragments arranged by size:
3572 2188 89
SalI G'TCGA_C
Cuts at: 2886 2886
Size: 5849
SapI GCTCTTCn'nnn_
Cuts at: 3872 4082 3872
Size: 210 5639
SbfI CC_TGCA'GG
Cuts at: 2896 2896
Size: 5849
ScaI AGT'ACT
Cuts at: 5366 5366
Size: 5849
SexAI A'CCwGG_T
Cuts at: 1757 1757
Size: 5849
SfcI C'TryA_G
Cuts at: 33 1427 1665 2233 2382 2892 3577 4545 4736
Size: 1394 238 568 149 510 685 968 191
Cuts at: 4736 33
Size: 1146
Fragments arranged by size:
1394 1146 968 685 568 510 238 191
149
SfoI GGC'GCC
Cuts at: 414 3281 3529 414
Size: 2867 248 2734
Fragments arranged by size:
2867 2734 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5849
SmaI CCC'GGG
Cuts at: 2871 2871
Size: 5849
SmlI C'TyrA_G
Cuts at: 316 454 754 1243 1447 1849 2338 2785 3004
Size: 138 300 489 204 402 489 447 219
Cuts at: 3004 3919 4633 4895 316
Size: 915 714 262 1270
Fragments arranged by size:
1270 915 714 489 489 447 402 300
262 219 204 138
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5849
SphI G_CATG'C
Cuts at: 1716 2391 2902 3402 1716
Size: 675 511 500 4163
Fragments arranged by size:
4163 675 511 500
StuI AGG'CCT
Cuts at: 266 817 1966 266
Size: 551 1149 4149
Fragments arranged by size:
4149 1149 551
StyI C'CwwG_G
Cuts at: 731 887 1352 2052 2983 3337 731
Size: 156 465 700 931 354 3243
Fragments arranged by size:
3243 931 700 465 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 460 546 550 576 1453 2157 3432 3565 4024
Size: 86 4 26 877 704 1275 133 459
Cuts at: 4024 4233 460
Size: 209 2076
Fragments arranged by size:
2076 1275 877 704 459 209 133 86
26 4
TatI w'GTAC_w
Cuts at: 1451 1571 2480 2552 5364 1451
Size: 120 909 72 2812 1936
Fragments arranged by size:
2812 1936 909 120 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 214 246 5366 5398 214
Size: 32 5120 32 665
Fragments arranged by size:
5120 665 32 32
Tth111I GACn'n_nGTC
Cuts at: 742 3646 742
Size: 2904 2945
XbaI T'CTAG_A
Cuts at: 2880 2880
Size: 5849
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1161 1917 3344 1161
Size: 756 1427 3666
Fragments arranged by size:
3666 1427 756
XhoI C'TCGA_G
Cuts at: 454 454
Size: 5849
XmaI C'CCGG_G
Cuts at: 2869 2869
Size: 5849
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AgeI AhdI AscI AvrII BaeI BfrBI BmtI
BsmBI BspEI BssHII BstEII BstZ17I Bsu36I EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PpuMI PsiI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI ZraI