Restriction map for
pF1KB9463
(Circular) (Six-base) MAPSORT of: pF1KB9463.seq Check: 9118 from: 1 to: 8729
pF1KB9463 8729 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1524 3130 3251 1524
Size: 1606 121 7002
Fragments arranged by size:
7002 1606 121
AccI GT'mk_AC
Cuts at: 2537 4020 4058 5767 2537
Size: 1483 38 1709 5499
Fragments arranged by size:
5499 1709 1483 38
Acc65I G'GTAC_C
Cuts at: 4275 5745 4275
Size: 1470 7259
AclI AA'CG_TT
Cuts at: 8569 8569
Size: 8729
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 727 2371 2566 2796 3283 3467 3738 4581
Size: 447 1644 195 230 487 184 271 843
Cuts at: 4581 5156 5603 6554 6986 7339 8001 280
Size: 575 447 951 432 353 662 1008
Fragments arranged by size:
1644 1008 951 843 662 575 487 447
447 432 353 271 230 195 184
AflII C'TTAA_G
Cuts at: 3879 3879
Size: 8729
AflIII A'CryG_T
Cuts at: 1413 2174 3989 6081 7881 8162 1413
Size: 761 1815 2092 1800 281 1980
Fragments arranged by size:
2092 1980 1815 1800 761 281
AleI CACnn'nnGTG
Cuts at: 1241 3631 1241
Size: 2390 6339
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 842 1733 1880 3649 842
Size: 891 147 1769 5922
Fragments arranged by size:
5922 1769 891 147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 8697
AlwNI CAG_nnn'CTG
Cuts at: 354 1396 1879 2748 4004 6034 7472 354
Size: 1042 483 869 1256 2030 1438 1611
Fragments arranged by size:
2030 1611 1438 1256 1042 869 483
ApaLI G'TGCA_C
Cuts at: 7567 7567
Size: 8729
ApoI r'AATT_y
Cuts at: 2934 3322 3464 3790 4093 4194 4643 4652 5733
Size: 388 142 326 303 101 449 9 1081
Cuts at: 5733 8082 8338 2934
Size: 2349 256 3325
Fragments arranged by size:
3325 2349 1081 449 388 326 303 256
142 101 9
AseI AT'TA_AT
Cuts at: 20 476 20
Size: 456 8273
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8729
AvaI C'yCGr_G
Cuts at: 1523 1721 3035 3539 5749 1523
Size: 198 1314 504 2210 4503
Fragments arranged by size:
4503 2210 1314 504 198
AvrII C'CTAG_G
Cuts at: 3735 3735
Size: 8729
BamHI G'GATC_C
Cuts at: 3680 5754 3680
Size: 2074 6655
BanI G'GyrC_C
Cuts at: 1072 1173 1567 3633 4275 5745 6159 6407 6442
Size: 101 394 2066 642 1470 414 248 35
Cuts at: 6442 1072
Size: 3359
Fragments arranged by size:
3359 2066 1470 642 414 394 248 101
35
BanII G_rGCy'C
Cuts at: 1293 3074 5348 5371 5743 6773 1293
Size: 1781 2274 23 372 1030 3249
Fragments arranged by size:
3249 2274 1781 1030 372 23
BbeI G_GCGC'C
Cuts at: 6163 6411 6163
Size: 248 8481
BbsI GAAGACnn'nnnn_
Cuts at: 3509 3645 5003 3509
Size: 136 1358 7235
Fragments arranged by size:
7235 1358 136
BbvCI CC'TCA_GC
Cuts at: 4202 4202
Size: 8729
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3104 3138 5428 5462 3104
Size: 34 2290 34 6371
Fragments arranged by size:
6371 2290 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5586 6627 7683 8729 5586
Size: 1041 1056 1046 5586
Fragments arranged by size:
5586 1056 1046 1041
BclI T'GATC_A
Cuts at: 2265 4584 6249 2265
Size: 2319 1665 4745
Fragments arranged by size:
4745 2319 1665
BglI GCCn_nnn'nGGC
Cuts at: 2923 2923
Size: 8729
BglII A'GATC_T
Cuts at: 6244 8281 6244
Size: 2037 6692
BlpI GC'TnA_GC
Cuts at: 2414 2510 5058 5841 2414
Size: 96 2548 783 5302
Fragments arranged by size:
5302 2548 783 96
Bme1580I G_kGCm'C
Cuts at: 1077 1572 2129 2959 3638 3669 6354 6447 7571
Size: 495 557 830 679 31 2685 93 1124
Cuts at: 7571 1077
Size: 2235
Fragments arranged by size:
2685 2235 1124 830 679 557 495 93
31
BmrI ACTGGGnnnn_n'
Cuts at: 2725 2888 3915 5941 6022 6357 8501 2725
Size: 163 1027 2026 81 335 2144 2953
Fragments arranged by size:
2953 2144 2026 1027 335 163 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5385 7096 5385
Size: 1711 7018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1460 1975 2434 4804 5165 5272 604
Size: 856 515 459 2370 361 107 4061
Fragments arranged by size:
4061 2370 856 515 459 361 107
Bpu10I CC'TnA_GC
Cuts at: 1779 2947 4202 1779
Size: 1168 1255 6306
Fragments arranged by size:
6306 1255 1168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5905 6784 7498 7796 5905
Size: 879 714 298 6838
Fragments arranged by size:
6838 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1127 2831 4795 4957 5416 8433 1127
Size: 1704 1964 162 459 3017 1423
Fragments arranged by size:
3017 1964 1704 1423 459 162
BsaAI yAC'GTr
Cuts at: 336 1140 6082 336
Size: 804 4942 2983
Fragments arranged by size:
4942 2983 804
BsaBI GATnn'nnATC
Cuts at: 6 6243 8280 6
Size: 6237 2037 455
Fragments arranged by size:
6237 2037 455
BsaHI Gr'CG_yC
Cuts at: 5033 6160 6408 7110 8606 5033
Size: 1127 248 702 1496 5156
Fragments arranged by size:
5156 1496 1127 702 248
BsaWI w'CCGG_w
Cuts at: 5918 6439 7117 7528 7675 7949 5918
Size: 521 678 411 147 274 6698
Fragments arranged by size:
6698 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 866 896 1124 1154 2351 2381 3144 3174 3240
Size: 30 228 30 1197 30 763 30 66
Cuts at: 3240 3270 5266 5296 866
Size: 30 1996 30 4299
Fragments arranged by size:
4299 1996 1197 763 228 66 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 593 902 2575 3245 4651 4918 4951 5284
Size: 407 309 1673 670 1406 267 33 333
Cuts at: 5284 5287 5377 186
Size: 3 90 3538
Fragments arranged by size:
3538 1673 1406 670 407 333 309 267
90 33 3
BseYI C'CCAG_C
Cuts at: 357 2734 2951 3303 5224 5233 6154 6912 7577
Size: 2377 217 352 1921 9 921 758 665
Cuts at: 7577 7985 357
Size: 408 1101
Fragments arranged by size:
2377 1921 1101 921 758 665 408 352
217 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 812 1010 1297 1693 3664 4901 5632 367
Size: 445 198 287 396 1971 1237 731 3464
Fragments arranged by size:
3464 1971 1237 731 445 396 287 198
BsiEI CG_ry'CG
Cuts at: 104 6271 6317 7547 104
Size: 6167 46 1230 1286
Fragments arranged by size:
6167 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 580 3222 5357 5371 5743 6521 6711 7571 580
Size: 2642 2135 14 372 778 190 860 1738
Fragments arranged by size:
2642 2135 1738 860 778 372 190 14
BsmI GAATG_Cn'
Cuts at: 3293 5624 3293
Size: 2331 6398
BsmBI CGTCTCn'nnnn_
Cuts at: 1495 2611 4992 5459 1495
Size: 1116 2381 467 4765
Fragments arranged by size:
4765 2381 1116 467
Bsp1286I G_dGCh'C
Cuts at: 580 1077 1293 1572 2129 2959 3074 3222 3638
Size: 497 216 279 557 830 115 148 416
Cuts at: 3638 3669 5348 5357 5371 5743 6354 6447 6521
Size: 31 1679 9 14 372 611 93 74
Cuts at: 6521 6711 6773 7571 580
Size: 190 62 798 1738
Fragments arranged by size:
1738 1679 830 798 611 557 497 416
372 279 216 190 148 115 93 74
62 31 14 9
BspHI T'CATG_A
Cuts at: 7161 7161
Size: 8729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 292 1524 2774 3130 3251 4975 5138 5779 5993
Size: 1232 1250 356 121 1724 163 641 214
Cuts at: 5993 6295 6676 292
Size: 302 381 2345
Fragments arranged by size:
2345 1724 1250 1232 641 381 356 302
214 163 121
BsrBI CCG'CTC
Cuts at: 50 406 1905 2413 5310 7021 7075 8559 8724
Size: 356 1499 508 2897 1711 54 1484 165
Cuts at: 8724 50
Size: 55
Fragments arranged by size:
2897 1711 1499 1484 508 356 165 55
54
BsrDI GCAATG_nn'
Cuts at: 207 413 1316 6641 207
Size: 206 903 5325 2295
Fragments arranged by size:
5325 2295 903 206
BsrFI r'CCGG_y
Cuts at: 6727 6908 6727
Size: 181 8548
BssSI C'ACGA_G
Cuts at: 4416 7000 7708 4416
Size: 2584 708 5437
Fragments arranged by size:
5437 2584 708
BstAPI GCAn_nnn'nTGC
Cuts at: 354 1879 5130 5619 6106 354
Size: 1525 3251 489 487 2977
Fragments arranged by size:
3251 2977 1525 489 487
BstBI TT'CG_AA
Cuts at: 4656 7090 4656
Size: 2434 6295
BstEII G'GTnAC_C
Cuts at: 2139 2565 2139
Size: 426 8303
BstXI CCAn_nnnn'nTGG
Cuts at: 654 780 4869 654
Size: 126 4089 4514
Fragments arranged by size:
4514 4089 126
BstYI r'GATC_y
Cuts at: 1 3347 3680 3731 4025 5754 6221 6244 6579
Size: 3346 333 51 294 1729 467 23 335
Cuts at: 6579 6825 7131 7143 7229 8281 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
3346 1729 1052 467 449 335 333 306
294 246 86 51 23 12
BstZ17I GTA'TAC
Cuts at: 4059 4059
Size: 8729
Bsu36I CC'TnA_GG
Cuts at: 2692 2699 2854 5246 2692
Size: 7 155 2392 6175
Fragments arranged by size:
6175 2392 155 7
BtgI C'CryG_G
Cuts at: 2531 5374 5534 2531
Size: 2843 160 5726
Fragments arranged by size:
5726 2843 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6679 6860 7023 6679
Size: 181 163 8385
Fragments arranged by size:
8385 181 163
BtsI GCAGTG_nn'
Cuts at: 578 1309 1705 1979 2477 2492 3408 3569 4913
Size: 731 396 274 498 15 916 161 1344
Cuts at: 4913 5972 578
Size: 1059 3335
Fragments arranged by size:
3335 1344 1059 916 731 498 396 274
161 15
ClaI AT'CG_AT
Cuts at: 983 4514 983
Size: 3531 5198
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1397 1432 1397
Size: 35 8694
DraI TTT'AAA
Cuts at: 5728 5728
Size: 8729
DraIII CAC_nnn'GTG
Cuts at: 1033 2904 5535 1033
Size: 1871 2631 4227
Fragments arranged by size:
4227 2631 1871
DrdI GACnn_nn'nnGTC
Cuts at: 6435 7779 6435
Size: 1344 7385
EaeI y'GGCC_r
Cuts at: 644 1233 1682 3185 4088 4208 4293 6314 6488
Size: 589 449 1503 903 120 85 2021 174
Cuts at: 6488 6879 6906 8251 644
Size: 391 27 1345 1122
Fragments arranged by size:
2021 1503 1345 1122 903 589 449 391
174 120 85 27
EagI C'GGCC_G
Cuts at: 6314 6314
Size: 8729
EarI CTCTTCn'nnn_
Cuts at: 357 413 863 2645 4502 4682 5132 5336 5594
Size: 56 450 1782 1857 180 450 204 258
Cuts at: 5594 5683 6752 6962 357
Size: 89 1069 210 2124
Fragments arranged by size:
2124 1857 1782 1069 450 450 258 210
204 180 89 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6937 7679 7825 8539 6937
Size: 742 146 714 7127
Fragments arranged by size:
7127 742 714 146
EcoICRI GAG'CTC
Cuts at: 5369 5741 5369
Size: 372 8357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 604 727 1460 1975 2371 2434 2566 2796
Size: 324 123 733 515 396 63 132 230
Cuts at: 2796 3283 3467 3738 4581 4804 5156 5165 5272
Size: 487 184 271 843 223 352 9 107
Cuts at: 5272 5603 6554 6986 7339 8001 280
Size: 331 951 432 353 662 1008
Fragments arranged by size:
1008 951 843 733 662 515 487 432
396 353 352 331 324 271 230 223
184 132 123 107 63 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2853 2853
Size: 8729
EcoO109I rG'GnC_Cy
Cuts at: 368 2703 3307 5868 368
Size: 2335 604 2561 3229
Fragments arranged by size:
3229 2561 2335 604
EcoRI G'AATT_C
Cuts at: 2934 5733 2934
Size: 2799 5930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2954 4589 6748 2954
Size: 1635 2159 4935
Fragments arranged by size:
4935 2159 1635
FspI TGC'GCA
Cuts at: 1226 3400 6510 1226
Size: 2174 3110 3445
Fragments arranged by size:
3445 3110 2174
HaeII r_GCGC'y
Cuts at: 1945 3141 6163 6411 7641 8412 1945
Size: 1196 3022 248 1230 771 2262
Fragments arranged by size:
3022 2262 1230 1196 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 709 741 754 786 1260 1292 1729 1761 2153
Size: 32 13 32 474 32 437 32 392
Cuts at: 2153 2185 2880 2912 4002 4034 4055 4087 4396
Size: 32 695 32 1090 32 21 32 309
Cuts at: 4396 4428 4507 4539 4956 4988 6571 6601 6603
Size: 32 79 32 417 32 1583 30 2
Cuts at: 6603 6633 709
Size: 30 2805
Fragments arranged by size:
2805 1583 1090 695 474 437 417 392
309 79 32 32 32 32 32 32
32 32 32 32 32 30 30 21
13 2
HincII GTy'rAC
Cuts at: 846 1007 2180 2340 2370 2538 2909 4021 5637
Size: 161 1173 160 30 168 371 1112 1616
Cuts at: 5637 5768 846
Size: 131 3807
Fragments arranged by size:
3807 1616 1173 1112 371 168 161 160
131 30
HindIII A'AGCT_T
Cuts at: 207 2284 3830 207
Size: 2077 1546 5106
Fragments arranged by size:
5106 2077 1546
HpaI GTT'AAC
Cuts at: 2370 2370
Size: 8729
KasI G'GCGC_C
Cuts at: 6159 6407 6159
Size: 248 8481
KpnI G_GTAC'C
Cuts at: 4279 5749 4279
Size: 1470 7259
MluI A'CGCG_T
Cuts at: 8162 8162
Size: 8729
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1043 2843 3133 3186 7488 7672 1043
Size: 1800 290 53 4302 184 2100
Fragments arranged by size:
4302 2100 1800 290 184 53
MscI TGG'CCA
Cuts at: 646 1235 3187 4090 4210 4295 6490 646
Size: 589 1952 903 120 85 2195 2885
Fragments arranged by size:
2885 2195 1952 903 589 120 85
MslI CAynn'nnrTG
Cuts at: 536 1241 1676 2134 2530 3434 3631 4841 6718
Size: 705 435 458 396 904 197 1210 1877
Cuts at: 6718 6845 536
Size: 127 2420
Fragments arranged by size:
2420 1877 1210 904 705 458 435 396
197 127
MspA1I CmG'CkG
Cuts at: 1650 3303 3419 4601 5376 5840 6154 6514 7298
Size: 1653 116 1182 775 464 314 360 784
Cuts at: 7298 7543 1650
Size: 245 2836
Fragments arranged by size:
2836 1653 1182 784 775 464 360 314
245 116
NaeI GCC'GGC
Cuts at: 6910 6910
Size: 8729
NarI GG'CG_CC
Cuts at: 6160 6408 6160
Size: 248 8481
NdeI CA'TA_TG
Cuts at: 1664 1664
Size: 8729
NgoMIV G'CCGG_C
Cuts at: 6908 6908
Size: 8729
NspI r_CATG'y
Cuts at: 922 1417 1813 2178 4735 5782 6282 7885 922
Size: 495 396 365 2557 1047 500 1603 1766
Fragments arranged by size:
2557 1766 1603 1047 500 495 396 365
PasI CC'CwG_GG
Cuts at: 1128 1128
Size: 8729
PciI A'CATG_T
Cuts at: 1413 2174 7881 1413
Size: 761 5707 2261
Fragments arranged by size:
5707 2261 761
PflMI CCAn_nnn'nTGG
Cuts at: 642 1050 1938 3147 4004 642
Size: 408 888 1209 857 5367
Fragments arranged by size:
5367 1209 888 857 408
PfoI T'CCnGG_A
Cuts at: 2747 3312 4028 4820 5181 2747
Size: 565 716 792 361 6295
Fragments arranged by size:
6295 792 716 565 361
PmeI GTTT'AAAC
Cuts at: 5728 5728
Size: 8729
PmlI CAC'GTG
Cuts at: 336 1140 336
Size: 804 7925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1456 1488 7175 7207 1456
Size: 32 5687 32 2978
Fragments arranged by size:
5687 2978 32 32
PpuMI rG'GwC_Cy
Cuts at: 368 2703 368
Size: 2335 6394
PshAI GACnn'nnGTC
Cuts at: 4688 4688
Size: 8729
PsiI TTA'TAA
Cuts at: 5671 5671
Size: 8729
PstI C_TGCA'G
Cuts at: 1463 1884 3698 5268 5776 6461 1463
Size: 421 1814 1570 508 685 3731
Fragments arranged by size:
3731 1814 1570 685 508 421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8729
PvuII CAG'CTG
Cuts at: 1650 3303 3419 4601 6154 6514 1650
Size: 1653 116 1182 1553 360 3865
Fragments arranged by size:
3865 1653 1553 1182 360 116
RsrII CG'GwC_CG
Cuts at: 6924 6924
Size: 8729
SacI G_AGCT'C
Cuts at: 5371 5743 5371
Size: 372 8357
SacII CC_GC'GG
Cuts at: 5377 5377
Size: 8729
SalI G'TCGA_C
Cuts at: 2536 4019 5766 2536
Size: 1483 1747 5499
Fragments arranged by size:
5499 1747 1483
SapI GCTCTTCn'nnn_
Cuts at: 413 5336 6752 6962 413
Size: 4923 1416 210 2180
Fragments arranged by size:
4923 2180 1416 210
SbfI CC_TGCA'GG
Cuts at: 3698 5776 3698
Size: 2078 6651
ScaI AGT'ACT
Cuts at: 3488 3556 4708 8246 3488
Size: 68 1152 3538 3971
Fragments arranged by size:
3971 3538 1152 68
SexAI A'CCwGG_T
Cuts at: 2900 2900
Size: 8729
SfcI C'TryA_G
Cuts at: 33 584 1459 1880 3694 3761 4736 5264 5772
Size: 551 875 421 1814 67 975 528 508
Cuts at: 5772 6457 7425 7616 33
Size: 685 968 191 1146
Fragments arranged by size:
1814 1146 975 968 875 685 551 528
508 421 191 67
SfoI GGC'GCC
Cuts at: 6161 6409 6161
Size: 248 8481
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8729
SmaI CCC'GGG
Cuts at: 1723 5751 1723
Size: 4028 4701
SmlI C'TyrA_G
Cuts at: 3879 5884 6799 7513 7775 3879
Size: 2005 915 714 262 4833
Fragments arranged by size:
4833 2005 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8729
SphI G_CATG'C
Cuts at: 5782 6282 5782
Size: 500 8229
SspI AAT'ATT
Cuts at: 474 550 5167 474
Size: 76 4617 4036
Fragments arranged by size:
4617 4036 76
StuI AGG'CCT
Cuts at: 2697 3182 3692 4424 2697
Size: 485 510 732 7002
Fragments arranged by size:
7002 732 510 485
StyI C'CwwG_G
Cuts at: 323 2498 2918 3228 3327 3735 4590 5863 6217
Size: 2175 420 310 99 408 855 1273 354
Cuts at: 6217 323
Size: 2835
Fragments arranged by size:
2835 2175 1273 855 420 408 354 310
99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2707 6312 6445 6904 7113 2707
Size: 3605 133 459 209 4323
Fragments arranged by size:
4323 3605 459 209 133
TatI w'GTAC_w
Cuts at: 3100 3486 3554 3615 4706 8244 3100
Size: 386 68 61 1091 3538 3585
Fragments arranged by size:
3585 3538 1091 386 68 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 866 898 1124 1156 5519 5551 8246 8278 866
Size: 32 226 32 4363 32 2695 32 1317
Fragments arranged by size:
4363 2695 1317 226 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6526 6526
Size: 8729
XbaI T'CTAG_A
Cuts at: 5760 5760
Size: 8729
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4422 6224 4422
Size: 1802 6927
XmaI C'CCGG_G
Cuts at: 1721 5749 1721
Size: 4028 4701
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AgeI AhdI ApaI AscI BaeI BfrBI
BmgBI BmtI BsiWI BspEI BsrGI BssHII EcoRV FseI
FspAI MfeI NcoI NheI NotI NruI NsiI PacI
PspOMI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI