Restriction map for
pF1KB9464
(Circular) (Six-base) MAPSORT of: pF1KB9464.seq Check: 8559 from: 1 to: 6683
pF1KB9464 6683 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AatII G_ACGT'C
Cuts at: 1368 1368
Size: 6683
AccI GT'mk_AC
Cuts at: 2009 3721 2009
Size: 1712 4971
Acc65I G'GTAC_C
Cuts at: 3699 3699
Size: 6683
AclI AA'CG_TT
Cuts at: 6523 6523
Size: 6683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1432 1628 1795 2786 3277 4508 4940 5293
Size: 960 196 167 991 491 1231 432 353
Cuts at: 5293 5955 472
Size: 662 1200
Fragments arranged by size:
1231 1200 991 960 662 491 432 353
196 167
AflIII A'CryG_T
Cuts at: 1187 2653 4035 5835 6116 1187
Size: 1466 1382 1800 281 1754
Fragments arranged by size:
1800 1754 1466 1382 281
AhdI GACnn_n'nnGTC
Cuts at: 2305 2566 2808 2305
Size: 261 242 6180
Fragments arranged by size:
6180 261 242
AleI CACnn'nnGTG
Cuts at: 2422 2422
Size: 6683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3341 3341
Size: 6683
AlwNI CAG_nnn'CTG
Cuts at: 1773 1974 2407 3493 3615 3988 5426 1773
Size: 201 433 1086 122 373 1438 3030
Fragments arranged by size:
3030 1438 1086 433 373 201 122
ApaI G_GGCC'C
Cuts at: 660 927 1022 660
Size: 267 95 6321
Fragments arranged by size:
6321 267 95
ApaLI G'TGCA_C
Cuts at: 2657 5521 2657
Size: 2864 3819
ApoI r'AATT_y
Cuts at: 2688 3687 6036 6292 2688
Size: 999 2349 256 3079
Fragments arranged by size:
3079 2349 999 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6683
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6683
AvaI C'yCGr_G
Cuts at: 318 743 803 944 997 1581 3703 318
Size: 425 60 141 53 584 2122 3298
Fragments arranged by size:
3298 2122 584 425 141 60 53
AvrII C'CTAG_G
Cuts at: 1003 1003
Size: 6683
BamHI G'GATC_C
Cuts at: 3708 3708
Size: 6683
BanI G'GyrC_C
Cuts at: 376 2896 3699 4113 4361 4396 376
Size: 2520 803 414 248 35 2663
Fragments arranged by size:
2663 2520 803 414 248 35
BanII G_rGCy'C
Cuts at: 660 927 991 1022 3021 3407 3697 4727 660
Size: 267 64 31 1999 386 290 1030 2616
Fragments arranged by size:
2616 1999 1030 386 290 267 64 31
BbeI G_GCGC'C
Cuts at: 2900 4117 4365 2900
Size: 1217 248 5218
Fragments arranged by size:
5218 1217 248
BbsI GAAGACnn'nnnn_
Cuts at: 1989 2225 2807 2882 1989
Size: 236 582 75 5790
Fragments arranged by size:
5790 582 236 75
BbvCI CC'TCA_GC
Cuts at: 679 679
Size: 6683
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1680 1714 90
Size: 34 1556 34 5059
Fragments arranged by size:
5059 1556 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3555 4581 5637 6683 3555
Size: 1026 1056 1046 3555
Fragments arranged by size:
3555 1056 1046 1026
BclI T'GATC_A
Cuts at: 2628 2790 4203 2628
Size: 162 1413 5108
Fragments arranged by size:
5108 1413 162
BglI GCCn_nnn'nGGC
Cuts at: 1008 1008
Size: 6683
BglII A'GATC_T
Cuts at: 1085 4198 6235 1085
Size: 3113 2037 1533
Fragments arranged by size:
3113 2037 1533
BlpI GC'TnA_GC
Cuts at: 3795 3795
Size: 6683
Bme1580I G_kGCm'C
Cuts at: 660 927 1022 2661 4308 4401 5525 660
Size: 267 95 1639 1647 93 1124 1818
Fragments arranged by size:
1818 1647 1639 1124 267 95 93
BmrI ACTGGGnnnn_n'
Cuts at: 2514 3895 3976 4311 6455 2514
Size: 1381 81 335 2144 2742
Fragments arranged by size:
2742 2144 1381 335 81
BmtI G_CTAG'C
Cuts at: 3041 3041
Size: 6683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5050 5050
Size: 6683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 790 1337 2184 2441 2541 3391 730
Size: 60 547 847 257 100 850 4022
Fragments arranged by size:
4022 850 847 547 257 100 60
Bpu10I CC'TnA_GC
Cuts at: 679 3527 679
Size: 2848 3835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 1383 3266 3859 4738 5452 5750 1144
Size: 239 1883 593 879 714 298 2077
Fragments arranged by size:
2077 1883 879 714 593 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 116 740 1072 1516 1757 2292 2766 6387 116
Size: 624 332 444 241 535 474 3621 412
Fragments arranged by size:
3621 624 535 474 444 412 332 241
BsaAI yAC'GTr
Cuts at: 4036 4036
Size: 6683
BsaBI GATnn'nnATC
Cuts at: 6 4197 6234 6
Size: 4191 2037 455
Fragments arranged by size:
4191 2037 455
BsaHI Gr'CG_yC
Cuts at: 642 1365 2897 4114 4362 5064 6560 642
Size: 723 1532 1217 248 702 1496 765
Fragments arranged by size:
1532 1496 1217 765 723 702 248
BsaWI w'CCGG_w
Cuts at: 253 560 689 1676 2429 3872 4393 5071 5482
Size: 307 129 987 753 1443 521 678 411
Cuts at: 5482 5629 5903 253
Size: 147 274 1033
Fragments arranged by size:
1443 1033 987 753 678 521 411 307
274 147 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1461 1491 1652 1682 2157 2187 2539 2569 2738
Size: 30 161 30 475 30 352 30 169
Cuts at: 2738 2768 3586 3616 1461
Size: 30 818 30 4528
Fragments arranged by size:
4528 818 475 352 169 161 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 134 594 1372 1553 2947 2952 2998 3042 3206
Size: 460 778 181 1394 5 46 44 164
Cuts at: 3206 3269 3307 3328 3607 3610 3613 134
Size: 63 38 21 279 3 3 3204
Fragments arranged by size:
3204 1394 778 460 279 181 164 63
46 44 38 21 5 3 3
BseYI C'CCAG_C
Cuts at: 617 991 1557 2031 2143 2256 3001 3072 3503
Size: 374 566 474 112 113 745 71 431
Cuts at: 3503 4108 4866 5531 5939 617
Size: 605 758 665 408 1361
Fragments arranged by size:
1361 758 745 665 605 566 474 431
408 374 113 112 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2995 2995
Size: 6683
BsiEI CG_ry'CG
Cuts at: 104 834 1015 4225 4271 5501 104
Size: 730 181 3210 46 1230 1286
Fragments arranged by size:
3210 1286 1230 730 181 46
BsiHKAI G_wGCw'C
Cuts at: 317 974 2661 3021 3407 3697 4475 4665 5525
Size: 657 1687 360 386 290 778 190 860
Cuts at: 5525 317
Size: 1475
Fragments arranged by size:
1687 1475 860 778 657 386 360 290
190
BsmI GAATG_Cn'
Cuts at: 1718 2174 3076 3401 3426 1718
Size: 456 902 325 25 4975
Fragments arranged by size:
4975 902 456 325 25
BsmBI CGTCTCn'nnnn_
Cuts at: 1356 1356
Size: 6683
Bsp1286I G_dGCh'C
Cuts at: 317 660 927 974 991 1022 2661 3021 3407
Size: 343 267 47 17 31 1639 360 386
Cuts at: 3407 3697 4308 4401 4475 4665 4727 5525 317
Size: 290 611 93 74 190 62 798 1475
Fragments arranged by size:
1639 1475 798 611 386 360 343 290
267 190 93 74 62 47 31 17
BspEI T'CCGG_A
Cuts at: 253 560 253
Size: 307 6376
BspHI T'CATG_A
Cuts at: 5115 5115
Size: 6683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3733 3947 4249 4630 3733
Size: 214 302 381 5786
Fragments arranged by size:
5786 381 302 214
BsrBI CCG'CTC
Cuts at: 50 654 672 714 774 894 941 2960 4975
Size: 604 18 42 60 120 47 2019 2015
Cuts at: 4975 5029 6513 6678 50
Size: 54 1484 165 55
Fragments arranged by size:
2019 2015 1484 604 165 120 60 55
54 47 42 18
BsrDI GCAATG_nn'
Cuts at: 1698 2342 4595 1698
Size: 644 2253 3786
Fragments arranged by size:
3786 2253 644
BsrFI r'CCGG_y
Cuts at: 111 584 2970 4681 4862 111
Size: 473 2386 1711 181 1932
Fragments arranged by size:
2386 1932 1711 473 181
BssHII G'CGCG_C
Cuts at: 736 736
Size: 6683
BssSI C'ACGA_G
Cuts at: 2980 3014 3510 4954 5662 2980
Size: 34 496 1444 708 4001
Fragments arranged by size:
4001 1444 708 496 34
BstAPI GCAn_nnn'nTGC
Cuts at: 3421 4060 3421
Size: 639 6044
BstBI TT'CG_AA
Cuts at: 5044 5044
Size: 6683
BstEII G'GTnAC_C
Cuts at: 1857 1857
Size: 6683
BstXI CCAn_nnnn'nTGG
Cuts at: 793 2185 2424 793
Size: 1392 239 5052
Fragments arranged by size:
5052 1392 239
BstYI r'GATC_y
Cuts at: 1 1085 2404 3708 4175 4198 4533 4779 5085
Size: 1084 1319 1304 467 23 335 246 306
Cuts at: 5085 5097 5183 6235 1
Size: 12 86 1052 449
Fragments arranged by size:
1319 1304 1084 1052 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2203 2203
Size: 6683
BtgI C'CryG_G
Cuts at: 126 383 597 696 756 828 2825 126
Size: 257 214 99 60 72 1997 3984
Fragments arranged by size:
3984 1997 257 214 99 72 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4633 4814 4977 4633
Size: 181 163 6339
Fragments arranged by size:
6339 181 163
BtsI GCAGTG_nn'
Cuts at: 254 3315 3588 3926 254
Size: 3061 273 338 3011
Fragments arranged by size:
3061 3011 338 273
DraI TTT'AAA
Cuts at: 3682 3682
Size: 6683
DraIII CAC_nnn'GTG
Cuts at: 2522 2522
Size: 6683
DrdI GACnn_nn'nnGTC
Cuts at: 361 4389 5733 361
Size: 4028 1344 1311
Fragments arranged by size:
4028 1344 1311
EaeI y'GGCC_r
Cuts at: 479 699 759 831 1012 2175 3645 4268 4442
Size: 220 60 72 181 1163 1470 623 174
Cuts at: 4442 4833 4860 6205 479
Size: 391 27 1345 957
Fragments arranged by size:
1470 1345 1163 957 623 391 220 181
174 72 60 27
EagI C'GGCC_G
Cuts at: 831 1012 4268 831
Size: 181 3256 3246
Fragments arranged by size:
3256 3246 181
EarI CTCTTCn'nnn_
Cuts at: 176 1052 4706 4916 176
Size: 876 3654 210 1943
Fragments arranged by size:
3654 1943 876 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1753 4891 5633 5779 6493 1753
Size: 3138 742 146 714 1943
Fragments arranged by size:
3138 1943 742 714 146
EcoICRI GAG'CTC
Cuts at: 3019 3405 3695 3019
Size: 386 290 6007
Fragments arranged by size:
6007 386 290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 730 790 1337 1432 1628 1795 2184 2441
Size: 258 60 547 95 196 167 389 257
Cuts at: 2441 2541 2786 3277 3391 4508 4940 5293 5955
Size: 100 245 491 114 1117 432 353 662
Cuts at: 5955 472
Size: 1200
Fragments arranged by size:
1200 1117 662 547 491 432 389 353
258 257 245 196 167 114 100 95
60
EcoNI CCTnn'n_nnAGG
Cuts at: 217 508 217
Size: 291 6392
EcoO109I rG'GnC_Cy
Cuts at: 461 675 717 777 923 949 1018 1029 1730
Size: 214 42 60 146 26 69 11 701
Cuts at: 1730 1749 3822 461
Size: 19 2073 3322
Fragments arranged by size:
3322 2073 701 214 146 69 60 42
26 19 11
EcoRI G'AATT_C
Cuts at: 3687 3687
Size: 6683
EcoRV GAT'ATC
Cuts at: 3456 3456
Size: 6683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4702 4702
Size: 6683
FspI TGC'GCA
Cuts at: 4464 4464
Size: 6683
HaeII r_GCGC'y
Cuts at: 2900 4117 4365 5595 6366 2900
Size: 1217 248 1230 771 3217
Fragments arranged by size:
3217 1230 1217 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 925 957 1174 1206 1288 1320 1353 1385 1461
Size: 32 217 32 82 32 33 32 76
Cuts at: 1461 1493 1979 2011 2196 2228 2291 2323 2389
Size: 32 486 32 185 32 63 32 66
Cuts at: 2389 2421 2552 2584 2775 2794 2807 2826 4525
Size: 32 131 32 191 19 13 19 1699
Cuts at: 4525 4555 4557 4587 925
Size: 30 2 30 3021
Fragments arranged by size:
3021 1699 486 217 191 185 131 82
76 66 63 33 32 32 32 32
32 32 32 32 32 32 30 30
19 19 13 2
HincII GTy'rAC
Cuts at: 3722 3722
Size: 6683
HindIII A'AGCT_T
Cuts at: 2641 2641
Size: 6683
KasI G'GCGC_C
Cuts at: 2896 4113 4361 2896
Size: 1217 248 5218
Fragments arranged by size:
5218 1217 248
KpnI G_GTAC'C
Cuts at: 3703 3703
Size: 6683
MfeI C'AATT_G
Cuts at: 296 2531 296
Size: 2235 4448
MluI A'CGCG_T
Cuts at: 6116 6116
Size: 6683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2048 2620 2627 5442 5626 2048
Size: 572 7 2815 184 3105
Fragments arranged by size:
3105 2815 572 184 7
MscI TGG'CCA
Cuts at: 2177 3647 4444 2177
Size: 1470 797 4416
Fragments arranged by size:
4416 1470 797
MslI CAynn'nnrTG
Cuts at: 112 382 2422 4672 4799 112
Size: 270 2040 2250 127 1996
Fragments arranged by size:
2250 2040 1996 270 127
MspA1I CmG'CkG
Cuts at: 128 617 830 2230 2940 3615 3794 4108 4468
Size: 489 213 1400 710 675 179 314 360
Cuts at: 4468 5252 5497 128
Size: 784 245 1314
Fragments arranged by size:
1400 1314 784 710 675 489 360 314
245 213 179
NaeI GCC'GGC
Cuts at: 586 4864 586
Size: 4278 2405
NarI GG'CG_CC
Cuts at: 2897 4114 4362 2897
Size: 1217 248 5218
Fragments arranged by size:
5218 1217 248
NcoI C'CATG_G
Cuts at: 383 597 2825 383
Size: 214 2228 4241
Fragments arranged by size:
4241 2228 214
NgoMIV G'CCGG_C
Cuts at: 584 4862 584
Size: 4278 2405
NheI G'CTAG_C
Cuts at: 3037 3037
Size: 6683
NspI r_CATG'y
Cuts at: 1191 2473 2811 3736 4236 5839 1191
Size: 1282 338 925 500 1603 2035
Fragments arranged by size:
2035 1603 1282 925 500 338
PciI A'CATG_T
Cuts at: 1187 5835 1187
Size: 4648 2035
PflMI CCAn_nnn'nTGG
Cuts at: 3493 3493
Size: 6683
PfoI T'CCnGG_A
Cuts at: 1456 3407 1456
Size: 1951 4732
PmeI GTTT'AAAC
Cuts at: 3682 3682
Size: 6683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5129 5161 5129
Size: 32 6651
PpuMI rG'GwC_Cy
Cuts at: 675 949 1029 1730 675
Size: 274 80 701 5628
Fragments arranged by size:
5628 701 274 80
PsiI TTA'TAA
Cuts at: 1933 1933
Size: 6683
PspOMI G'GGCC_C
Cuts at: 656 923 1018 656
Size: 267 95 6321
Fragments arranged by size:
6321 267 95
PstI C_TGCA'G
Cuts at: 3730 4415 3730
Size: 685 5998
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6683
PvuII CAG'CTG
Cuts at: 617 2230 3615 4108 4468 617
Size: 1613 1385 493 360 2832
Fragments arranged by size:
2832 1613 1385 493 360
RsrII CG'GwC_CG
Cuts at: 4878 4878
Size: 6683
SacI G_AGCT'C
Cuts at: 3021 3407 3697 3021
Size: 386 290 6007
Fragments arranged by size:
6007 386 290
SacII CC_GC'GG
Cuts at: 129 831 129
Size: 702 5981
SalI G'TCGA_C
Cuts at: 3720 3720
Size: 6683
SanDI GG'GwC_CC
Cuts at: 949 949
Size: 6683
SapI GCTCTTCn'nnn_
Cuts at: 176 4706 4916 176
Size: 4530 210 1943
Fragments arranged by size:
4530 1943 210
SbfI CC_TGCA'GG
Cuts at: 3730 3730
Size: 6683
ScaI AGT'ACT
Cuts at: 6200 6200
Size: 6683
SfcI C'TryA_G
Cuts at: 33 570 900 1237 3726 4411 5379 5570 33
Size: 537 330 337 2489 685 968 191 1146
Fragments arranged by size:
2489 1146 968 685 537 337 330 191
SfoI GGC'GCC
Cuts at: 2898 4115 4363 2898
Size: 1217 248 5218
Fragments arranged by size:
5218 1217 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6683
SmaI CCC'GGG
Cuts at: 320 805 3705 320
Size: 485 2900 3298
Fragments arranged by size:
3298 2900 485
SmlI C'TyrA_G
Cuts at: 1159 1398 3281 3838 4753 5467 5729 1159
Size: 239 1883 557 915 714 262 2113
Fragments arranged by size:
2113 1883 915 714 557 262 239
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6683
SphI G_CATG'C
Cuts at: 2473 3736 4236 2473
Size: 1263 500 4920
Fragments arranged by size:
4920 1263 500
SspI AAT'ATT
Cuts at: 3359 3359
Size: 6683
StyI C'CwwG_G
Cuts at: 383 597 1003 2825 3095 3817 4171 383
Size: 214 406 1822 270 722 354 2895
Fragments arranged by size:
2895 1822 722 406 354 270 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1453 1488 2533 4266 4399 4858 5067 1453
Size: 35 1045 1733 133 459 209 3069
Fragments arranged by size:
3069 1733 1045 459 209 133 35
TatI w'GTAC_w
Cuts at: 200 911 6198 200
Size: 711 5287 685
Fragments arranged by size:
5287 711 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 233 265 1652 1684 2157 2189 3499 3531 6200
Size: 32 1387 32 473 32 1310 32 2669
Cuts at: 6200 6232 233
Size: 32 684
Fragments arranged by size:
2669 1387 1310 684 473 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4480 4480
Size: 6683
XbaI T'CTAG_A
Cuts at: 3714 3714
Size: 6683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 280 501 3339 4178 280
Size: 221 2838 839 2785
Fragments arranged by size:
2838 2785 839 221
XmaI C'CCGG_G
Cuts at: 318 803 3703 318
Size: 485 2900 3298
Fragments arranged by size:
3298 2900 485
ZraI GAC'GTC
Cuts at: 1366 1366
Size: 6683
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AloI AscI BaeI BfrBI
BmgBI BsiWI BsrGI BstZ17I ClaI CspCI FseI FspAI
HpaI NdeI NotI NruI NsiI PacI PasI PmlI
PshAI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI