Restriction map for
pF1KB9466
(Circular) (Six-base) MAPSORT of: pF1KB9466.seq Check: 3348 from: 1 to: 5444
pF1KB9466 5444 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 502 658 2482 502
Size: 156 1824 3464
Fragments arranged by size:
3464 1824 156
Acc65I G'GTAC_C
Cuts at: 2460 2460
Size: 5444
AclI AA'CG_TT
Cuts at: 1678 5284 1678
Size: 3606 1838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 1376 1537 1751 2059 2111 2167 3269 3701
Size: 792 161 214 308 52 56 1102 432
Cuts at: 3701 4054 4716 584
Size: 353 662 1312
Fragments arranged by size:
1312 1102 792 662 432 353 308 214
161 56 52
AflIII A'CryG_T
Cuts at: 1799 2796 4596 4877 1799
Size: 997 1800 281 2366
Fragments arranged by size:
2366 1800 997 281
AleI CACnn'nnGTG
Cuts at: 544 544
Size: 5444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1838 1838
Size: 5444
AlwNI CAG_nnn'CTG
Cuts at: 2749 4187 2749
Size: 1438 4006
ApaLI G'TGCA_C
Cuts at: 4282 4282
Size: 5444
ApoI r'AATT_y
Cuts at: 1508 1604 2350 2448 4797 5053 1508
Size: 96 746 98 2349 256 1899
Fragments arranged by size:
2349 1899 746 256 98 96
AseI AT'TA_AT
Cuts at: 20 1994 20
Size: 1974 3470
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5444
AvaI C'yCGr_G
Cuts at: 447 2356 2464 447
Size: 1909 108 3427
Fragments arranged by size:
3427 1909 108
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1084 1117 1084
Size: 33 5411
BamHI G'GATC_C
Cuts at: 2469 2469
Size: 5444
BanI G'GyrC_C
Cuts at: 631 1205 2460 2874 3122 3157 631
Size: 574 1255 414 248 35 2918
Fragments arranged by size:
2918 1255 574 414 248 35
BanII G_rGCy'C
Cuts at: 2458 3488 2458
Size: 1030 4414
BbeI G_GCGC'C
Cuts at: 2878 3126 2878
Size: 248 5196
BbsI GAAGACnn'nnnn_
Cuts at: 230 291 806 1543 230
Size: 61 515 737 4131
Fragments arranged by size:
4131 737 515 61
BciVI GTATCCnnnnn_n'
Cuts at: 3342 4398 5444 3342
Size: 1056 1046 3342
Fragments arranged by size:
3342 1056 1046
BclI T'GATC_A
Cuts at: 2964 2964
Size: 5444
BfrBI ATG'CAT
Cuts at: 2283 2283
Size: 5444
BglI GCCn_nnn'nGGC
Cuts at: 629 629
Size: 5444
BglII A'GATC_T
Cuts at: 336 891 2959 4996 336
Size: 555 2068 2037 784
Fragments arranged by size:
2068 2037 784 555
BlpI GC'TnA_GC
Cuts at: 2556 2556
Size: 5444
Bme1580I G_kGCm'C
Cuts at: 1003 3069 3162 4286 1003
Size: 2066 93 1124 2161
Fragments arranged by size:
2161 2066 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 905 1006 2656 2737 3072 5216 905
Size: 101 1650 81 335 2144 1133
Fragments arranged by size:
2144 1650 1133 335 101 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2027 3811 2027
Size: 1784 3660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1049 1109 1238 2214 1049
Size: 60 129 976 4279
Fragments arranged by size:
4279 976 129 60
Bpu10I CC'TnA_GC
Cuts at: 167 167
Size: 5444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2620 3499 4213 4511 2620
Size: 879 714 298 3553
Fragments arranged by size:
3553 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1296 5148 1296
Size: 3852 1592
BsaAI yAC'GTr
Cuts at: 2797 2797
Size: 5444
BsaBI GATnn'nnATC
Cuts at: 6 2958 4995 6
Size: 2952 2037 455
Fragments arranged by size:
2952 2037 455
BsaHI Gr'CG_yC
Cuts at: 2875 3123 3825 5321 2875
Size: 248 702 1496 2998
Fragments arranged by size:
2998 1496 702 248
BsaWI w'CCGG_w
Cuts at: 858 2633 3154 3832 4243 4390 4664 858
Size: 1775 521 678 411 147 274 1638
Fragments arranged by size:
1775 1638 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 206 236 881 911 206
Size: 30 645 30 4739
Fragments arranged by size:
4739 645 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 224 227 776 1310 1323 224
Size: 3 549 534 13 4345
Fragments arranged by size:
4345 549 534 13 3
BseYI C'CCAG_C
Cuts at: 151 1294 1527 2869 3627 4292 4700 151
Size: 1143 233 1342 758 665 408 895
Fragments arranged by size:
1342 1143 895 758 665 408 233
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 951 951
Size: 5444
BsiEI CG_ry'CG
Cuts at: 104 2986 3032 4262 104
Size: 2882 46 1230 1286
Fragments arranged by size:
2882 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1381 2458 3236 3426 4286 1381
Size: 1077 778 190 860 2539
Fragments arranged by size:
2539 1077 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 721 721
Size: 5444
Bsp1286I G_dGCh'C
Cuts at: 1003 1381 2458 3069 3162 3236 3426 3488 4286
Size: 378 1077 611 93 74 190 62 798
Cuts at: 4286 1003
Size: 2161
Fragments arranged by size:
2161 1077 798 611 378 190 93 74
62
BspHI T'CATG_A
Cuts at: 1784 3876 1784
Size: 2092 3352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1973 2494 2708 3010 3391 1973
Size: 521 214 302 381 4026
Fragments arranged by size:
4026 521 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3736 3790 5274 5439 50
Size: 3686 54 1484 165 55
Fragments arranged by size:
3686 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 327 482 651 3356 327
Size: 155 169 2705 2415
Fragments arranged by size:
2705 2415 169 155
BsrFI r'CCGG_y
Cuts at: 3442 3623 3442
Size: 181 5263
BssSI C'ACGA_G
Cuts at: 3715 4423 3715
Size: 708 4736
BstAPI GCAn_nnn'nTGC
Cuts at: 541 2821 541
Size: 2280 3164
BstBI TT'CG_AA
Cuts at: 1512 3805 1512
Size: 2293 3151
BstXI CCAn_nnnn'nTGG
Cuts at: 1216 1216
Size: 5444
BstYI r'GATC_y
Cuts at: 1 336 891 1213 2469 2936 2959 3294 3540
Size: 335 555 322 1256 467 23 335 246
Cuts at: 3540 3846 3858 3944 4996 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1256 1052 555 467 449 335 335 322
306 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 659 659
Size: 5444
Bsu36I CC'TnA_GG
Cuts at: 2135 2135
Size: 5444
BtgI C'CryG_G
Cuts at: 107 107
Size: 5444
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3394 3575 3738 3394
Size: 181 163 5100
Fragments arranged by size:
5100 181 163
BtsI GCAGTG_nn'
Cuts at: 162 539 922 1457 1835 2687 162
Size: 377 383 535 378 852 2919
Fragments arranged by size:
2919 852 535 383 378 377
ClaI AT'CG_AT
Cuts at: 1502 1502
Size: 5444
DraI TTT'AAA
Cuts at: 2443 2443
Size: 5444
DrdI GACnn_nn'nnGTC
Cuts at: 3150 4494 3150
Size: 1344 4100
EaeI y'GGCC_r
Cuts at: 3029 3203 3594 3621 4966 3029
Size: 174 391 27 1345 3507
Fragments arranged by size:
3507 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3029 3029
Size: 5444
EarI CTCTTCn'nnn_
Cuts at: 2084 2130 2143 3467 3677 2084
Size: 46 13 1324 210 3851
Fragments arranged by size:
3851 1324 210 46 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3652 4394 4540 5254 3652
Size: 742 146 714 3842
Fragments arranged by size:
3842 742 714 146
EcoICRI GAG'CTC
Cuts at: 2456 2456
Size: 5444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 1049 1109 1238 1376 1537 1751 2059 2111
Size: 465 60 129 138 161 214 308 52
Cuts at: 2111 2167 2214 3269 3701 4054 4716 584
Size: 56 47 1055 432 353 662 1312
Fragments arranged by size:
1312 1055 662 465 432 353 308 214
161 138 129 60 56 52 47
EcoO109I rG'GnC_Cy
Cuts at: 713 2583 713
Size: 1870 3574
EcoRI G'AATT_C
Cuts at: 1604 2350 2448 1604
Size: 746 98 4600
Fragments arranged by size:
4600 746 98
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3463 3463
Size: 5444
FspI TGC'GCA
Cuts at: 326 3225 326
Size: 2899 2545
HaeII r_GCGC'y
Cuts at: 2878 3126 4356 5127 2878
Size: 248 1230 771 3195
Fragments arranged by size:
3195 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1300 1332 3286 3316 3318 3348 1300
Size: 32 1954 30 2 30 3396
Fragments arranged by size:
3396 1954 32 30 30 2
HincII GTy'rAC
Cuts at: 503 2483 503
Size: 1980 3464
HindIII A'AGCT_T
Cuts at: 466 1020 1388 466
Size: 554 368 4522
Fragments arranged by size:
4522 554 368
KasI G'GCGC_C
Cuts at: 2874 3122 2874
Size: 248 5196
KpnI G_GTAC'C
Cuts at: 2464 2464
Size: 5444
MluI A'CGCG_T
Cuts at: 4877 4877
Size: 5444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1694 4203 4387 1694
Size: 2509 184 2751
Fragments arranged by size:
2751 2509 184
MscI TGG'CCA
Cuts at: 3205 3205
Size: 5444
MslI CAynn'nnrTG
Cuts at: 544 590 1874 3433 3560 544
Size: 46 1284 1559 127 2428
Fragments arranged by size:
2428 1559 1284 127 46
MspA1I CmG'CkG
Cuts at: 2247 2555 2869 3229 4013 4258 2247
Size: 308 314 360 784 245 3433
Fragments arranged by size:
3433 784 360 314 308 245
NaeI GCC'GGC
Cuts at: 3625 3625
Size: 5444
NarI GG'CG_CC
Cuts at: 2875 3123 2875
Size: 248 5196
NcoI C'CATG_G
Cuts at: 107 107
Size: 5444
NgoMIV G'CCGG_C
Cuts at: 3623 3623
Size: 5444
NsiI A_TGCA'T
Cuts at: 2285 2285
Size: 5444
NspI r_CATG'y
Cuts at: 1535 1803 2025 2046 2497 2997 4600 1535
Size: 268 222 21 451 500 1603 2379
Fragments arranged by size:
2379 1603 500 451 268 222 21
PasI CC'CwG_GG
Cuts at: 973 973
Size: 5444
PciI A'CATG_T
Cuts at: 1799 4596 1799
Size: 2797 2647
PfoI T'CCnGG_A
Cuts at: 1026 1903 1026
Size: 877 4567
PmeI GTTT'AAAC
Cuts at: 2443 2443
Size: 5444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 3890
Size: 32 5412
PpuMI rG'GwC_Cy
Cuts at: 713 713
Size: 5444
PstI C_TGCA'G
Cuts at: 1898 2491 3176 1898
Size: 593 685 4166
Fragments arranged by size:
4166 685 593
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5444
PvuII CAG'CTG
Cuts at: 2869 3229 2869
Size: 360 5084
RsrII CG'GwC_CG
Cuts at: 3639 3639
Size: 5444
SacI G_AGCT'C
Cuts at: 2458 2458
Size: 5444
SalI G'TCGA_C
Cuts at: 501 2481 501
Size: 1980 3464
SanDI GG'GwC_CC
Cuts at: 713 713
Size: 5444
SapI GCTCTTCn'nnn_
Cuts at: 2143 3467 3677 2143
Size: 1324 210 3910
Fragments arranged by size:
3910 1324 210
SbfI CC_TGCA'GG
Cuts at: 2491 2491
Size: 5444
ScaI AGT'ACT
Cuts at: 1900 4961 1900
Size: 3061 2383
SexAI A'CCwGG_T
Cuts at: 1851 1851
Size: 5444
SfcI C'TryA_G
Cuts at: 33 1619 1894 2487 3172 4140 4331 33
Size: 1586 275 593 685 968 191 1146
Fragments arranged by size:
1586 1146 968 685 593 275 191
SfoI GGC'GCC
Cuts at: 2876 3124 2876
Size: 248 5196
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5444
SmaI CCC'GGG
Cuts at: 2466 2466
Size: 5444
SmlI C'TyrA_G
Cuts at: 2599 3514 4228 4490 2599
Size: 915 714 262 3553
Fragments arranged by size:
3553 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5444
SphI G_CATG'C
Cuts at: 1535 2497 2997 1535
Size: 962 500 3982
Fragments arranged by size:
3982 962 500
SspI AAT'ATT
Cuts at: 2398 2398
Size: 5444
StyI C'CwwG_G
Cuts at: 107 742 1209 2578 2932 107
Size: 635 467 1369 354 2619
Fragments arranged by size:
2619 1369 635 467 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3027 3160 3619 3828 3027
Size: 133 459 209 4643
Fragments arranged by size:
4643 459 209 133
TatI w'GTAC_w
Cuts at: 1898 4959 1898
Size: 3061 2383
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 206 238 4961 4993 206
Size: 32 4723 32 657
Fragments arranged by size:
4723 657 32 32
Tth111I GACn'n_nGTC
Cuts at: 3241 3241
Size: 5444
XbaI T'CTAG_A
Cuts at: 2475 2475
Size: 5444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1368 1772 2939 1368
Size: 404 1167 3873
Fragments arranged by size:
3873 1167 404
XmaI C'CCGG_G
Cuts at: 2464 2464
Size: 5444
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BbvCI BcgI BmgBI BmtI BsiWI BsmI
BspEI BsrGI BssHII BstEII CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI