Restriction map for
pF1KB9467
(Circular) (Six-base) MAPSORT of: pF1KB9467.seq Check: 4465 from: 1 to: 6656
pF1KB9467 6656 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 606 606
Size: 6656
AccI GT'mk_AC
Cuts at: 1688 1865 3094 3694 1688
Size: 177 1229 600 4650
Fragments arranged by size:
4650 1229 600 177
Acc65I G'GTAC_C
Cuts at: 3672 3672
Size: 6656
AclI AA'CG_TT
Cuts at: 3370 6496 3370
Size: 3126 3530
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2133 2433 2797 3052 4481 4913 5266 5928 2133
Size: 300 364 255 1429 432 353 662 2861
Fragments arranged by size:
2861 1429 662 432 364 353 300 255
AfeI AGC'GCT
Cuts at: 161 161
Size: 6656
AflII C'TTAA_G
Cuts at: 2844 2844
Size: 6656
AflIII A'CryG_T
Cuts at: 379 496 4008 5808 6089 379
Size: 117 3512 1800 281 946
Fragments arranged by size:
3512 1800 946 281 117
AleI CACnn'nnGTG
Cuts at: 1556 2011 1556
Size: 455 6201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2331 2331
Size: 6656
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 2024
Size: 32 6624
AlwNI CAG_nnn'CTG
Cuts at: 910 3961 5399 910
Size: 3051 1438 2167
Fragments arranged by size:
3051 2167 1438
ApaLI G'TGCA_C
Cuts at: 1643 2989 5494 1643
Size: 1346 2505 2805
Fragments arranged by size:
2805 2505 1346
ApoI r'AATT_y
Cuts at: 393 684 697 1077 1187 1385 1851 2894 2918
Size: 291 13 380 110 198 466 1043 24
Cuts at: 2918 2946 3016 3228 3341 3660 6009 6265 393
Size: 28 70 212 113 319 2349 256 784
Fragments arranged by size:
2349 1043 784 466 380 319 291 256
212 198 113 110 70 28 24 13
AseI AT'TA_AT
Cuts at: 20 20
Size: 6656
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6656
AvaI C'yCGr_G
Cuts at: 1478 1744 2320 3676 1478
Size: 266 576 1356 4458
Fragments arranged by size:
4458 1356 576 266
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3103 3136 3103
Size: 33 6623
BamHI G'GATC_C
Cuts at: 1924 3681 1924
Size: 1757 4899
BanI G'GyrC_C
Cuts at: 2023 2276 3672 4086 4334 4369 2023
Size: 253 1396 414 248 35 4310
Fragments arranged by size:
4310 1396 414 253 248 35
BanII G_rGCy'C
Cuts at: 2348 3644 3670 4700 2348
Size: 1296 26 1030 4304
Fragments arranged by size:
4304 1296 1030 26
BbeI G_GCGC'C
Cuts at: 4090 4338 4090
Size: 248 6408
BbsI GAAGACnn'nnnn_
Cuts at: 1345 2439 1345
Size: 1094 5562
BciVI GTATCCnnnnn_n'
Cuts at: 4554 5610 6656 4554
Size: 1056 1046 4554
Fragments arranged by size:
4554 1056 1046
BclI T'GATC_A
Cuts at: 996 4176 996
Size: 3180 3476
BglI GCCn_nnn'nGGC
Cuts at: 1748 1748
Size: 6656
BglII A'GATC_T
Cuts at: 2932 4171 6208 2932
Size: 1239 2037 3380
Fragments arranged by size:
3380 2037 1239
BlpI GC'TnA_GC
Cuts at: 3768 3768
Size: 6656
Bme1580I G_kGCm'C
Cuts at: 516 1647 1891 2993 4281 4374 5498 516
Size: 1131 244 1102 1288 93 1124 1674
Fragments arranged by size:
1674 1288 1131 1124 1102 244 93
BmgBI CAC'GTC
Cuts at: 1629 1629
Size: 6656
BmrI ACTGGGnnnn_n'
Cuts at: 3137 3868 3949 4284 6428 3137
Size: 731 81 335 2144 3365
Fragments arranged by size:
3365 2144 731 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 508 5023 508
Size: 4515 2141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 503 1338 1466 1877 1936 2229 333
Size: 170 835 128 411 59 293 4760
Fragments arranged by size:
4760 835 411 293 170 128 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2519 3832 4711 5425 5723 2519
Size: 1313 879 714 298 3452
Fragments arranged by size:
3452 1313 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 211 3625 6360 211
Size: 3414 2735 507
Fragments arranged by size:
3414 2735 507
BsaAI yAC'GTr
Cuts at: 4009 4009
Size: 6656
BsaBI GATnn'nnATC
Cuts at: 6 2229 3176 4170 6207 6
Size: 2223 947 994 2037 455
Fragments arranged by size:
2223 2037 994 947 455
BsaHI Gr'CG_yC
Cuts at: 2824 4087 4335 5037 6533 2824
Size: 1263 248 702 1496 2947
Fragments arranged by size:
2947 1496 1263 702 248
BsaWI w'CCGG_w
Cuts at: 229 3845 4366 5044 5455 5602 5876 229
Size: 3616 521 678 411 147 274 1009
Fragments arranged by size:
3616 1009 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1232 1466 1232
Size: 234 6422
BseYI C'CCAG_C
Cuts at: 1168 1293 4081 4839 5504 5912 1168
Size: 125 2788 758 665 408 1912
Fragments arranged by size:
2788 1912 758 665 408 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1280 1319 283
Size: 997 39 5620
Fragments arranged by size:
5620 997 39
BsiEI CG_ry'CG
Cuts at: 104 4198 4244 5474 104
Size: 4094 46 1230 1286
Fragments arranged by size:
4094 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1476 1634 1647 2327 2993 3644 3670 4448 4638
Size: 158 13 680 666 651 26 778 190
Cuts at: 4638 5498 1476
Size: 860 2634
Fragments arranged by size:
2634 860 778 680 666 651 190 158
26 13
BsmI GAATG_Cn'
Cuts at: 413 2127 413
Size: 1714 4942
BsmBI CGTCTCn'nnnn_
Cuts at: 2831 2831
Size: 6656
Bsp1286I G_dGCh'C
Cuts at: 516 1476 1634 1647 1891 2327 2348 2993 3644
Size: 960 158 13 244 436 21 645 651
Cuts at: 3644 3670 4281 4374 4448 4638 4700 5498 516
Size: 26 611 93 74 190 62 798 1674
Fragments arranged by size:
1674 960 798 651 645 611 436 244
190 158 93 74 62 26 21 13
BspHI T'CATG_A
Cuts at: 5088 5088
Size: 6656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 606 3292 3706 3920 4222 4603 606
Size: 2686 414 214 302 381 2659
Fragments arranged by size:
2686 2659 414 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4948 5002 6486 6651 50
Size: 4898 54 1484 165 55
Fragments arranged by size:
4898 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 526 4568 526
Size: 4042 2614
BsrFI r'CCGG_y
Cuts at: 1492 2676 4654 4835 1492
Size: 1184 1978 181 3313
Fragments arranged by size:
3313 1978 1184 181
BsrGI T'GTAC_A
Cuts at: 702 1652 702
Size: 950 5706
BssSI C'ACGA_G
Cuts at: 4927 5635 4927
Size: 708 5948
BstAPI GCAn_nnn'nTGC
Cuts at: 4033 4033
Size: 6656
BstBI TT'CG_AA
Cuts at: 5017 5017
Size: 6656
BstEII G'GTnAC_C
Cuts at: 1558 1558
Size: 6656
BstXI CCAn_nnnn'nTGG
Cuts at: 474 844 1960 474
Size: 370 1116 5170
Fragments arranged by size:
5170 1116 370
BstYI r'GATC_y
Cuts at: 1 1924 2932 3681 4148 4171 4506 4752 5058
Size: 1923 1008 749 467 23 335 246 306
Cuts at: 5058 5070 5156 6208 1
Size: 12 86 1052 449
Fragments arranged by size:
1923 1052 1008 749 467 449 335 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1866 3095 1866
Size: 1229 5427
BtgI C'CryG_G
Cuts at: 107 107
Size: 6656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2750 4606 4787 4950 2750
Size: 1856 181 163 4456
Fragments arranged by size:
4456 1856 181 163
BtsI GCAGTG_nn'
Cuts at: 266 371 1307 3899 266
Size: 105 936 2592 3023
Fragments arranged by size:
3023 2592 936 105
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2454 2489 2454
Size: 35 6621
DraI TTT'AAA
Cuts at: 179 1991 2259 2756 3655 179
Size: 1812 268 497 899 3180
Fragments arranged by size:
3180 1812 899 497 268
DrdI GACnn_nn'nnGTC
Cuts at: 4362 5706 4362
Size: 1344 5312
EaeI y'GGCC_r
Cuts at: 1350 1494 2189 4241 4415 4806 4833 6178 1350
Size: 144 695 2052 174 391 27 1345 1828
Fragments arranged by size:
2052 1828 1345 695 391 174 144 27
EagI C'GGCC_G
Cuts at: 4241 4241
Size: 6656
EarI CTCTTCn'nnn_
Cuts at: 1275 1677 2967 4679 4889 1275
Size: 402 1290 1712 210 3042
Fragments arranged by size:
3042 1712 1290 402 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4864 5606 5752 6466 4864
Size: 742 146 714 5054
Fragments arranged by size:
5054 742 714 146
EcoICRI GAG'CTC
Cuts at: 3642 3668 3642
Size: 26 6630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 503 1338 1466 1877 1936 2133 2229 2433
Size: 170 835 128 411 59 197 96 204
Cuts at: 2433 2797 3052 4481 4913 5266 5928 333
Size: 364 255 1429 432 353 662 1061
Fragments arranged by size:
1429 1061 835 662 432 411 364 353
255 204 197 170 128 96 59
EcoO109I rG'GnC_Cy
Cuts at: 721 849 1741 1967 3795 721
Size: 128 892 226 1828 3582
Fragments arranged by size:
3582 1828 892 226 128
EcoRI G'AATT_C
Cuts at: 1851 3660 1851
Size: 1809 4847
EcoRV GAT'ATC
Cuts at: 3174 3174
Size: 6656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3594 4675 3594
Size: 1081 5575
FspI TGC'GCA
Cuts at: 4437 4437
Size: 6656
HaeII r_GCGC'y
Cuts at: 163 4090 4338 5568 6339 163
Size: 3927 248 1230 771 480
Fragments arranged by size:
3927 1230 771 480 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 946 978 3597 3629 4498 4528 4530 4560 946
Size: 32 2619 32 869 30 2 30 3042
Fragments arranged by size:
3042 2619 869 32 32 30 30 2
HincII GTy'rAC
Cuts at: 363 2115 2361 2576 2886 3695 363
Size: 1752 246 215 310 809 3324
Fragments arranged by size:
3324 1752 809 310 246 215
HindIII A'AGCT_T
Cuts at: 2081 2081
Size: 6656
HpaI GTT'AAC
Cuts at: 2576 2576
Size: 6656
KasI G'GCGC_C
Cuts at: 4086 4334 4086
Size: 248 6408
KpnI G_GTAC'C
Cuts at: 3676 3676
Size: 6656
MfeI C'AATT_G
Cuts at: 1778 1778
Size: 6656
MluI A'CGCG_T
Cuts at: 6089 6089
Size: 6656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1138 2541 5415 5599 1138
Size: 1403 2874 184 2195
Fragments arranged by size:
2874 2195 1403 184
MscI TGG'CCA
Cuts at: 1352 2191 4417 1352
Size: 839 2226 3591
Fragments arranged by size:
3591 2226 839
MslI CAynn'nnrTG
Cuts at: 519 1556 1801 2011 4645 4772 519
Size: 1037 245 210 2634 127 2403
Fragments arranged by size:
2634 2403 1037 245 210 127
MspA1I CmG'CkG
Cuts at: 138 144 2530 3397 3767 4081 4441 5225 5470
Size: 6 2386 867 370 314 360 784 245
Cuts at: 5470 138
Size: 1324
Fragments arranged by size:
2386 1324 867 784 370 360 314 245
6
NaeI GCC'GGC
Cuts at: 4837 4837
Size: 6656
NarI GG'CG_CC
Cuts at: 4087 4335 4087
Size: 248 6408
NcoI C'CATG_G
Cuts at: 107 107
Size: 6656
NgoMIV G'CCGG_C
Cuts at: 4835 4835
Size: 6656
NspI r_CATG'y
Cuts at: 383 500 2702 3709 4209 5812 383
Size: 117 2202 1007 500 1603 1227
Fragments arranged by size:
2202 1603 1227 1007 500 117
PciI A'CATG_T
Cuts at: 379 496 5808 379
Size: 117 5312 1227
Fragments arranged by size:
5312 1227 117
PflMI CCAn_nnn'nTGG
Cuts at: 3344 3344
Size: 6656
PmeI GTTT'AAAC
Cuts at: 3655 3655
Size: 6656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5102 5134 5102
Size: 32 6624
PpuMI rG'GwC_Cy
Cuts at: 849 1967 849
Size: 1118 5538
PshAI GACnn'nnGTC
Cuts at: 563 1330 2387 563
Size: 767 1057 4832
Fragments arranged by size:
4832 1057 767
PsiI TTA'TAA
Cuts at: 274 3511 274
Size: 3237 3419
PstI C_TGCA'G
Cuts at: 1394 2285 3259 3402 3703 4388 1394
Size: 891 974 143 301 685 3662
Fragments arranged by size:
3662 974 891 685 301 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6656
PvuII CAG'CTG
Cuts at: 2530 3397 4081 4441 2530
Size: 867 684 360 4745
Fragments arranged by size:
4745 867 684 360
RsrII CG'GwC_CG
Cuts at: 4851 4851
Size: 6656
SacI G_AGCT'C
Cuts at: 3644 3670 3644
Size: 26 6630
SalI G'TCGA_C
Cuts at: 3693 3693
Size: 6656
SapI GCTCTTCn'nnn_
Cuts at: 4679 4889 4679
Size: 210 6446
SbfI CC_TGCA'GG
Cuts at: 3703 3703
Size: 6656
ScaI AGT'ACT
Cuts at: 1957 6173 1957
Size: 4216 2440
SfcI C'TryA_G
Cuts at: 33 1390 2075 2281 3255 3270 3398 3699 4384
Size: 1357 685 206 974 15 128 301 685
Cuts at: 4384 5352 5543 33
Size: 968 191 1146
Fragments arranged by size:
1357 1146 974 968 685 685 301 206
191 128 15
SfoI GGC'GCC
Cuts at: 4088 4336 4088
Size: 248 6408
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6656
SmaI CCC'GGG
Cuts at: 3678 3678
Size: 6656
SmlI C'TyrA_G
Cuts at: 2534 2844 3811 4726 5440 5702 2534
Size: 310 967 915 714 262 3488
Fragments arranged by size:
3488 967 915 714 310 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6656
SphI G_CATG'C
Cuts at: 3709 4209 3709
Size: 500 6156
SspI AAT'ATT
Cuts at: 224 224
Size: 6656
StuI AGG'CCT
Cuts at: 710 710
Size: 6656
StyI C'CwwG_G
Cuts at: 107 667 1970 2441 3790 4144 107
Size: 560 1303 471 1349 354 2619
Fragments arranged by size:
2619 1349 1303 560 471 354
SwaI ATTT'AAAT
Cuts at: 1991 1991
Size: 6656
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1285 4239 4372 4831 5040 1285
Size: 2954 133 459 209 2901
Fragments arranged by size:
2954 2901 459 209 133
TatI w'GTAC_w
Cuts at: 702 1652 1955 3381 6171 702
Size: 950 303 1426 2790 1187
Fragments arranged by size:
2790 1426 1187 950 303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 486 518 6173 6205 486
Size: 32 5655 32 937
Fragments arranged by size:
5655 937 32 32
Tth111I GACn'n_nGTC
Cuts at: 4453 4453
Size: 6656
XbaI T'CTAG_A
Cuts at: 3687 3687
Size: 6656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 287 2470 4151 287
Size: 2183 1681 2792
Fragments arranged by size:
2792 2183 1681
XmaI C'CCGG_G
Cuts at: 3676 3676
Size: 6656
XmnI GAAnn'nnTTC
Cuts at: 3264 3264
Size: 6656
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AgeI AhdI ApaI AscI AvrII BbvCI BcgI
BfrBI BmtI Bpu10I BsaXI BsiWI BspEI BssHII Bsu36I
ClaI DraIII EcoNI FseI FspAI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
XhoI ZraI