(Circular) (Six-base) MAPSORT of: pF1KB9468.seq Check: 4394 from: 1 to: 9545 pF1KB9468 9545 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AatII G_ACGT'C Cuts at: 115 115 Size: 9545 AccI GT'mk_AC Cuts at: 5122 5372 6583 5122 Size: 250 1211 8084 Fragments arranged by size: 8084 1211 250 Acc65I G'GTAC_C Cuts at: 6561 6561 Size: 9545 AclI AA'CG_TT Cuts at: 2215 4220 9385 2215 Size: 2005 5165 2375 Fragments arranged by size: 5165 2375 2005 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1229 1291 1734 2221 2280 2993 3157 7370 7802 Size: 62 443 487 59 713 164 4213 432 Cuts at: 7802 8155 8817 1229 Size: 353 662 1957 Fragments arranged by size: 4213 1957 713 662 487 443 432 353 164 62 59 AfeI AGC'GCT Cuts at: 4964 4964 Size: 9545 AflII C'TTAA_G Cuts at: 427 1834 427 Size: 1407 8138 AflIII A'CryG_T Cuts at: 4272 6897 8697 8978 4272 Size: 2625 1800 281 4839 Fragments arranged by size: 4839 2625 1800 281 AleI CACnn'nnGTG Cuts at: 441 3259 5194 441 Size: 2818 1935 4792 Fragments arranged by size: 4792 2818 1935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 4651 4957 4651 Size: 306 9239 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2712 2744 2712 Size: 32 9513 AlwNI CAG_nnn'CTG Cuts at: 129 1543 3016 4438 4680 6315 6501 6850 8288 Size: 1414 1473 1422 242 1635 186 349 1438 Cuts at: 8288 129 Size: 1386 Fragments arranged by size: 1635 1473 1438 1422 1414 1386 349 242 186 ApaLI G'TGCA_C Cuts at: 3373 8383 3373 Size: 5010 4535 ApoI r'AATT_y Cuts at: 945 1592 2124 2491 3313 4336 4441 4648 5145 Size: 647 532 367 822 1023 105 207 497 Cuts at: 5145 6214 6549 8898 9154 945 Size: 1069 335 2349 256 1336 Fragments arranged by size: 2349 1336 1069 1023 822 647 532 497 367 335 256 207 105 AseI AT'TA_AT Cuts at: 20 566 1855 3868 20 Size: 546 1289 2013 5697 Fragments arranged by size: 5697 2013 1289 546 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 9545 AvaI C'yCGr_G Cuts at: 726 5564 6565 726 Size: 4838 1001 3706 Fragments arranged by size: 4838 3706 1001 BamHI G'GATC_C Cuts at: 2393 3339 6570 2393 Size: 946 3231 5368 Fragments arranged by size: 5368 3231 946 BanI G'GyrC_C Cuts at: 2623 3786 4289 6561 6975 7223 7258 2623 Size: 1163 503 2272 414 248 35 4910 Fragments arranged by size: 4910 2272 1163 503 414 248 35 BanII G_rGCy'C Cuts at: 2432 3254 5687 5745 6559 7589 2432 Size: 822 2433 58 814 1030 4388 Fragments arranged by size: 4388 2433 1030 822 814 58 BbeI G_GCGC'C Cuts at: 4293 6979 7227 4293 Size: 2686 248 6611 Fragments arranged by size: 6611 2686 248 BbsI GAAGACnn'nnnn_ Cuts at: 2286 2573 2827 3874 4676 5017 2286 Size: 287 254 1047 802 341 6814 Fragments arranged by size: 6814 1047 802 341 287 254 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 4399 4433 6245 6279 4399 Size: 34 1812 34 7665 Fragments arranged by size: 7665 1812 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7443 8499 9545 7443 Size: 1056 1046 7443 Fragments arranged by size: 7443 1056 1046 BclI T'GATC_A Cuts at: 995 1884 4916 7065 995 Size: 889 3032 2149 3475 Fragments arranged by size: 3475 3032 2149 889 BfrBI ATG'CAT Cuts at: 6251 6251 Size: 9545 BglII A'GATC_T Cuts at: 660 1464 3681 7060 9097 660 Size: 804 2217 3379 2037 1108 Fragments arranged by size: 3379 2217 2037 1108 804 BlpI GC'TnA_GC Cuts at: 735 1561 6657 735 Size: 826 5096 3623 Fragments arranged by size: 5096 3623 826 Bme1580I G_kGCm'C Cuts at: 2415 3377 3634 4036 7170 7263 8387 2415 Size: 962 257 402 3134 93 1124 3573 Fragments arranged by size: 3573 3134 1124 962 402 257 93 BmgBI CAC'GTC Cuts at: 4275 4275 Size: 9545 BmrI ACTGGGnnnn_n' Cuts at: 2392 4333 5173 6757 6838 7173 9317 2392 Size: 1941 840 1584 81 335 2144 2620 Fragments arranged by size: 2620 2144 1941 1584 840 335 81 BmtI G_CTAG'C Cuts at: 328 1301 328 Size: 973 8572 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3748 5289 5376 5706 6541 7912 3748 Size: 1541 87 330 835 1371 5381 Fragments arranged by size: 5381 1541 1371 835 330 87 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 469 635 2843 2917 3622 3893 4492 4780 5195 Size: 166 2208 74 705 271 599 288 415 Cuts at: 5195 5302 5346 5494 5743 5785 6511 469 Size: 107 44 148 249 42 726 3503 Fragments arranged by size: 3503 2208 726 705 599 415 288 271 249 166 148 107 74 44 42 Bpu10I CC'TnA_GC Cuts at: 117 117 Size: 9545 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2765 6721 7600 8314 8612 2765 Size: 3956 879 714 298 3698 Fragments arranged by size: 3956 3698 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 4756 5364 9249 4756 Size: 608 3885 5052 Fragments arranged by size: 5052 3885 608 BsaAI yAC'GTr Cuts at: 6898 6898 Size: 9545 BsaBI GATnn'nnATC Cuts at: 6 858 4501 7059 9096 6 Size: 852 3643 2558 2037 455 Fragments arranged by size: 3643 2558 2037 852 455 BsaHI Gr'CG_yC Cuts at: 112 4290 6976 7224 7926 9422 112 Size: 4178 2686 248 702 1496 235 Fragments arranged by size: 4178 2686 1496 702 248 235 BsaWI w'CCGG_w Cuts at: 4053 6734 7255 7933 8344 8491 8765 4053 Size: 2681 521 678 411 147 274 4833 Fragments arranged by size: 4833 2681 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 128 158 4091 4121 128 Size: 30 3933 30 5552 Fragments arranged by size: 5552 3933 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 2197 2200 3013 3961 4342 5239 6324 2197 Size: 3 813 948 381 897 1085 5418 Fragments arranged by size: 5418 1085 948 897 813 381 3 BseYI C'CCAG_C Cuts at: 122 2024 5300 5308 5744 6128 6970 7728 8393 Size: 1902 3276 8 436 384 842 758 665 Cuts at: 8393 8801 122 Size: 408 866 Fragments arranged by size: 3276 1902 866 842 758 665 436 408 384 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1990 2569 4349 4805 5083 5174 1990 Size: 579 1780 456 278 91 6361 Fragments arranged by size: 6361 1780 579 456 278 91 BsiEI CG_ry'CG Cuts at: 104 7087 7133 8363 104 Size: 6983 46 1230 1286 Fragments arranged by size: 6983 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 755 1028 2240 2432 3377 3540 4286 5687 6559 Size: 273 1212 192 945 163 746 1401 872 Cuts at: 6559 7337 7527 8387 755 Size: 778 190 860 1913 Fragments arranged by size: 1913 1401 1212 945 872 860 778 746 273 192 190 163 BsmI GAATG_Cn' Cuts at: 3407 5450 3407 Size: 2043 7502 BsmBI CGTCTCn'nnnn_ Cuts at: 1179 2247 5527 1179 Size: 1068 3280 5197 Fragments arranged by size: 5197 3280 1068 Bsp1286I G_dGCh'C Cuts at: 755 1028 2240 2415 2432 3254 3377 3540 3634 Size: 273 1212 175 17 822 123 163 94 Cuts at: 3634 4036 4286 5687 5745 6559 7170 7263 7337 Size: 402 250 1401 58 814 611 93 74 Cuts at: 7337 7527 7589 8387 755 Size: 190 62 798 1913 Fragments arranged by size: 1913 1401 1212 822 814 798 611 402 273 250 190 175 163 123 94 93 74 62 58 17 BspEI T'CCGG_A Cuts at: 4053 4053 Size: 9545 BspHI T'CATG_A Cuts at: 1622 7977 1622 Size: 6355 3190 BspMI ACCTGCnnnn'nnnn_ Cuts at: 5384 6595 6809 7111 7492 5384 Size: 1211 214 302 381 7437 Fragments arranged by size: 7437 1211 381 302 214 BsrBI CCG'CTC Cuts at: 50 7837 7891 9375 9540 50 Size: 7787 54 1484 165 55 Fragments arranged by size: 7787 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 500 2810 5933 7457 500 Size: 2310 3123 1524 2588 Fragments arranged by size: 3123 2588 2310 1524 BsrFI r'CCGG_y Cuts at: 5971 7543 7724 5971 Size: 1572 181 7792 Fragments arranged by size: 7792 1572 181 BssHII G'CGCG_C Cuts at: 4839 4841 5246 4839 Size: 2 405 9138 Fragments arranged by size: 9138 405 2 BssSI C'ACGA_G Cuts at: 523 7816 8524 523 Size: 7293 708 1544 Fragments arranged by size: 7293 1544 708 BstAPI GCAn_nnn'nTGC Cuts at: 1155 3660 6922 1155 Size: 2505 3262 3778 Fragments arranged by size: 3778 3262 2505 BstBI TT'CG_AA Cuts at: 7906 7906 Size: 9545 BstEII G'GTnAC_C Cuts at: 5268 5268 Size: 9545 BstXI CCAn_nnnn'nTGG Cuts at: 5355 5355 Size: 9545 BstYI r'GATC_y Cuts at: 1 660 1464 2133 2393 3239 3339 3681 5166 Size: 659 804 669 260 846 100 342 1485 Cuts at: 5166 6570 7037 7060 7395 7641 7947 7959 8045 Size: 1404 467 23 335 246 306 12 86 Cuts at: 8045 9097 1 Size: 1052 449 Fragments arranged by size: 1485 1404 1052 846 804 669 659 467 449 342 335 306 260 246 100 86 23 12 BstZ17I GTA'TAC Cuts at: 5123 5123 Size: 9545 Bsu36I CC'TnA_GG Cuts at: 206 6341 206 Size: 6135 3410 BtgI C'CryG_G Cuts at: 926 2453 3278 4799 5813 6272 926 Size: 1527 825 1521 1014 459 4199 Fragments arranged by size: 4199 1527 1521 1014 825 459 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 807 6237 7495 7676 7839 807 Size: 5430 1258 181 163 2513 Fragments arranged by size: 5430 2513 1258 181 163 BtsI GCAGTG_nn' Cuts at: 648 2351 2763 3558 4565 5189 6788 648 Size: 1703 412 795 1007 624 1599 3405 Fragments arranged by size: 3405 1703 1599 1007 795 624 412 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1883 1918 1883 Size: 35 9510 DraI TTT'AAA Cuts at: 2261 3332 3561 4647 4736 6544 2261 Size: 1071 229 1086 89 1808 5262 Fragments arranged by size: 5262 1808 1086 1071 229 89 DraIII CAC_nnn'GTG Cuts at: 3535 4327 5982 3535 Size: 792 1655 7098 Fragments arranged by size: 7098 1655 792 DrdI GACnn_nn'nnGTC Cuts at: 7251 8595 7251 Size: 1344 8201 EaeI y'GGCC_r Cuts at: 215 1598 2450 5054 5291 5973 6269 7130 7304 Size: 1383 852 2604 237 682 296 861 174 Cuts at: 7304 7695 7722 9067 215 Size: 391 27 1345 693 Fragments arranged by size: 2604 1383 1345 861 852 693 682 391 296 237 174 27 EagI C'GGCC_G Cuts at: 7130 7130 Size: 9545 EarI CTCTTCn'nnn_ Cuts at: 260 1148 1307 1859 2420 3340 4220 4742 5675 Size: 888 159 552 561 920 880 522 933 Cuts at: 5675 6203 7568 7778 260 Size: 528 1365 210 2027 Fragments arranged by size: 2027 1365 933 920 888 880 561 552 528 522 210 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3973 6292 7753 8495 8641 9355 3973 Size: 2319 1461 742 146 714 4163 Fragments arranged by size: 4163 2319 1461 742 714 146 EcoICRI GAG'CTC Cuts at: 2430 5685 6557 2430 Size: 3255 872 5418 Fragments arranged by size: 5418 3255 872 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 469 635 1229 1291 1734 2221 2280 2843 2917 Size: 166 594 62 443 487 59 563 74 Cuts at: 2917 2993 3157 3622 3893 4492 4780 5195 5302 Size: 76 164 465 271 599 288 415 107 Cuts at: 5302 5346 5494 5743 5785 6511 7370 7802 8155 Size: 44 148 249 42 726 859 432 353 Cuts at: 8155 8817 469 Size: 662 1197 Fragments arranged by size: 1197 859 726 662 599 594 563 487 465 443 432 415 353 288 271 249 166 164 148 107 76 74 62 59 44 42 EcoO109I rG'GnC_Cy Cuts at: 2937 5727 5846 6684 2937 Size: 2790 119 838 5798 Fragments arranged by size: 5798 2790 838 119 EcoRI G'AATT_C Cuts at: 945 1592 6549 945 Size: 647 4957 3941 Fragments arranged by size: 4957 3941 647 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 289 1257 1270 1296 2053 6090 7564 289 Size: 968 13 26 757 4037 1474 2270 Fragments arranged by size: 4037 2270 1474 968 757 26 13 FspI TGC'GCA Cuts at: 7326 7326 Size: 9545 HaeII r_GCGC'y Cuts at: 790 4293 4966 5290 6979 7227 8457 9228 790 Size: 3503 673 324 1689 248 1230 771 1107 Fragments arranged by size: 3503 1689 1230 1107 771 673 324 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 128 160 793 825 888 920 1758 1790 1876 Size: 32 633 32 63 32 838 32 86 Cuts at: 1876 1908 1936 1968 2173 2205 2769 2801 2961 Size: 32 28 32 205 32 564 32 160 Cuts at: 2961 2993 3673 3705 4091 4123 4707 4739 6156 Size: 32 680 32 386 32 584 32 1417 Cuts at: 6156 6188 7387 7417 7419 7449 128 Size: 32 1199 30 2 30 2224 Fragments arranged by size: 2224 1417 1199 838 680 633 584 564 386 205 160 86 63 32 32 32 32 32 32 32 32 32 32 32 32 32 30 30 28 2 HincII GTy'rAC Cuts at: 3183 5909 6584 3183 Size: 2726 675 6144 Fragments arranged by size: 6144 2726 675 HindIII A'AGCT_T Cuts at: 2323 3484 4491 4856 5131 2323 Size: 1161 1007 365 275 6737 Fragments arranged by size: 6737 1161 1007 365 275 KasI G'GCGC_C Cuts at: 4289 6975 7223 4289 Size: 2686 248 6611 Fragments arranged by size: 6611 2686 248 KpnI G_GTAC'C Cuts at: 6565 6565 Size: 9545 MluI A'CGCG_T Cuts at: 8978 8978 Size: 9545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 356 3684 5754 8304 8488 356 Size: 3328 2070 2550 184 1413 Fragments arranged by size: 3328 2550 2070 1413 184 MscI TGG'CCA Cuts at: 1600 5056 5293 6271 7306 1600 Size: 3456 237 978 1035 3839 Fragments arranged by size: 3839 3456 1035 978 237 MslI CAynn'nnrTG Cuts at: 441 2668 3259 4013 4912 5194 7534 7661 441 Size: 2227 591 754 899 282 2340 127 2325 Fragments arranged by size: 2340 2325 2227 899 754 591 282 127 MspA1I CmG'CkG Cuts at: 129 1846 3259 6186 6656 6970 7330 8114 8359 Size: 1717 1413 2927 470 314 360 784 245 Cuts at: 8359 129 Size: 1315 Fragments arranged by size: 2927 1717 1413 1315 784 470 360 314 245 NaeI GCC'GGC Cuts at: 5973 7726 5973 Size: 1753 7792 NarI GG'CG_CC Cuts at: 4290 6976 7224 4290 Size: 2686 248 6611 Fragments arranged by size: 6611 2686 248 NcoI C'CATG_G Cuts at: 926 3278 4799 5813 6272 926 Size: 2352 1521 1014 459 4199 Fragments arranged by size: 4199 2352 1521 1014 459 NgoMIV G'CCGG_C Cuts at: 5971 7724 5971 Size: 1753 7792 NheI G'CTAG_C Cuts at: 324 1297 324 Size: 973 8572 NsiI A_TGCA'T Cuts at: 6253 6253 Size: 9545 NspI r_CATG'y Cuts at: 1158 6598 7098 8701 1158 Size: 5440 500 1603 2002 Fragments arranged by size: 5440 2002 1603 500 PacI TTA_AT'TAA Cuts at: 1859 1859 Size: 9545 PciI A'CATG_T Cuts at: 8697 8697 Size: 9545 PflMI CCAn_nnn'nTGG Cuts at: 1293 3916 1293 Size: 2623 6922 PfoI T'CCnGG_A Cuts at: 3737 4277 5002 5720 6218 3737 Size: 540 725 718 498 7064 Fragments arranged by size: 7064 725 718 540 498 PmeI GTTT'AAAC Cuts at: 6544 6544 Size: 9545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3937 3969 7991 8023 3937 Size: 32 4022 32 5459 Fragments arranged by size: 5459 4022 32 32 PsiI TTA'TAA Cuts at: 3364 6139 3364 Size: 2775 6770 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 627 659 2891 2923 6088 6120 627 Size: 32 2232 32 3165 32 4052 Fragments arranged by size: 4052 3165 2232 32 32 32 PstI C_TGCA'G Cuts at: 801 1034 2346 2758 2777 3752 4991 5402 6592 Size: 233 1312 412 19 975 1239 411 1190 Cuts at: 6592 7277 801 Size: 685 3069 Fragments arranged by size: 3069 1312 1239 1190 975 685 412 411 233 19 PvuI CG_AT'CG Cuts at: 104 104 Size: 9545 PvuII CAG'CTG Cuts at: 129 3259 6186 6970 7330 129 Size: 3130 2927 784 360 2344 Fragments arranged by size: 3130 2927 2344 784 360 RsrII CG'GwC_CG Cuts at: 7740 7740 Size: 9545 SacI G_AGCT'C Cuts at: 2432 5687 6559 2432 Size: 3255 872 5418 Fragments arranged by size: 5418 3255 872 SalI G'TCGA_C Cuts at: 6582 6582 Size: 9545 SapI GCTCTTCn'nnn_ Cuts at: 2420 5675 7568 7778 2420 Size: 3255 1893 210 4187 Fragments arranged by size: 4187 3255 1893 210 SbfI CC_TGCA'GG Cuts at: 6592 6592 Size: 9545 ScaI AGT'ACT Cuts at: 3170 4513 9062 3170 Size: 1343 4549 3653 Fragments arranged by size: 4549 3653 1343 SexAI A'CCwGG_T Cuts at: 6008 6008 Size: 9545 SfcI C'TryA_G Cuts at: 33 797 1030 2342 2754 2773 3058 3748 4202 Size: 764 233 1312 412 19 285 690 454 Cuts at: 4202 4597 4987 5398 6588 7273 8241 8432 33 Size: 395 390 411 1190 685 968 191 1146 Fragments arranged by size: 1312 1190 1146 968 764 690 685 454 412 411 395 390 285 233 191 19 SfoI GGC'GCC Cuts at: 4291 6977 7225 4291 Size: 2686 248 6611 Fragments arranged by size: 6611 2686 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 9545 SmaI CCC'GGG Cuts at: 728 6567 728 Size: 5839 3706 SmlI C'TyrA_G Cuts at: 427 1834 2780 6700 7615 8329 8591 427 Size: 1407 946 3920 915 714 262 1381 Fragments arranged by size: 3920 1407 1381 946 915 714 262 SpeI A'CTAG_T Cuts at: 67 5485 67 Size: 5418 4127 SphI G_CATG'C Cuts at: 6598 7098 6598 Size: 500 9045 SspI AAT'ATT Cuts at: 536 4049 536 Size: 3513 6032 StuI AGG'CCT Cuts at: 4742 4742 Size: 9545 StyI C'CwwG_G Cuts at: 335 926 1053 3278 3626 4799 5387 5813 6272 Size: 591 127 2225 348 1173 588 426 459 Cuts at: 6272 6679 7033 335 Size: 407 354 2847 Fragments arranged by size: 2847 2225 1173 591 588 459 426 407 354 348 127 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2167 4315 7128 7261 7720 7929 2167 Size: 2148 2813 133 459 209 3783 Fragments arranged by size: 3783 2813 2148 459 209 133 TatI w'GTAC_w Cuts at: 253 3168 4511 9060 253 Size: 2915 1343 4549 738 Fragments arranged by size: 4549 2915 1343 738 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5552 5584 9062 9094 5552 Size: 32 3478 32 6003 Fragments arranged by size: 6003 3478 32 32 Tth111I GACn'n_nGTC Cuts at: 7342 7342 Size: 9545 XbaI T'CTAG_A Cuts at: 172 2676 3351 3684 6576 172 Size: 2504 675 333 2892 3141 Fragments arranged by size: 3141 2892 2504 675 333 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2405 2941 7040 2405 Size: 536 4099 4910 Fragments arranged by size: 4910 4099 536 XmaI C'CCGG_G Cuts at: 726 6565 726 Size: 5839 3706 XmnI GAAnn'nnTTC Cuts at: 1379 2659 3473 3692 4547 5450 6425 1379 Size: 1280 814 219 855 903 975 4499 Fragments arranged by size: 4499 1280 975 903 855 814 219 ZraI GAC'GTC Cuts at: 113 113 Size: 9545 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PacI PciI PflMI PfoI PmeI PpiI PsiI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AgeI AhdI ApaI AscI AvrII BaeI BbvCI BglI BsiWI BsrGI ClaI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NotI NruI PasI PmlI PpuMI PshAI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI