Restriction map for pF1KB9468
 (Circular) (Six-base) MAPSORT of: pF1KB9468.seq  Check: 4394  from: 1  to: 9545



pF1KB9468  9545 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AatII G_ACGT'C

Cuts at:    115     115 
   Size:       9545
 
AccI GT'mk_AC

Cuts at:   5122    5372    6583    5122 
   Size:        250    1211    8084

  Fragments arranged by size: 

                8084    1211     250
 
Acc65I G'GTAC_C

Cuts at:   6561    6561 
   Size:       9545
 
AclI AA'CG_TT

Cuts at:   2215    4220    9385    2215 
   Size:       2005    5165    2375

  Fragments arranged by size: 

                5165    2375    2005
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1229    1291    1734    2221    2280    2993    3157    7370    7802 
   Size:         62     443     487      59     713     164    4213     432

Cuts at:   7802    8155    8817    1229 
   Size:        353     662    1957

  Fragments arranged by size: 

                4213    1957     713     662     487     443     432     353
                 164      62      59
 
AfeI AGC'GCT

Cuts at:   4964    4964 
   Size:       9545
 
AflII C'TTAA_G

Cuts at:    427    1834     427 
   Size:       1407    8138
 
AflIII A'CryG_T

Cuts at:   4272    6897    8697    8978    4272 
   Size:       2625    1800     281    4839

  Fragments arranged by size: 

                4839    2625    1800     281
 
AleI CACnn'nnGTG

Cuts at:    441    3259    5194     441 
   Size:       2818    1935    4792

  Fragments arranged by size: 

                4792    2818    1935
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   4651    4957    4651 
   Size:        306    9239
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2712    2744    2712 
   Size:         32    9513
 
AlwNI CAG_nnn'CTG

Cuts at:    129    1543    3016    4438    4680    6315    6501    6850    8288 
   Size:       1414    1473    1422     242    1635     186     349    1438

Cuts at:   8288     129 
   Size:       1386

  Fragments arranged by size: 

                1635    1473    1438    1422    1414    1386     349     242
                 186
 
ApaLI G'TGCA_C

Cuts at:   3373    8383    3373 
   Size:       5010    4535
 
ApoI r'AATT_y

Cuts at:    945    1592    2124    2491    3313    4336    4441    4648    5145 
   Size:        647     532     367     822    1023     105     207     497

Cuts at:   5145    6214    6549    8898    9154     945 
   Size:       1069     335    2349     256    1336

  Fragments arranged by size: 

                2349    1336    1069    1023     822     647     532     497
                 367     335     256     207     105
 
AseI AT'TA_AT

Cuts at:     20     566    1855    3868      20 
   Size:        546    1289    2013    5697

  Fragments arranged by size: 

                5697    2013    1289     546
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9545
 
AvaI C'yCGr_G

Cuts at:    726    5564    6565     726 
   Size:       4838    1001    3706

  Fragments arranged by size: 

                4838    3706    1001
 
BamHI G'GATC_C

Cuts at:   2393    3339    6570    2393 
   Size:        946    3231    5368

  Fragments arranged by size: 

                5368    3231     946
 
BanI G'GyrC_C

Cuts at:   2623    3786    4289    6561    6975    7223    7258    2623 
   Size:       1163     503    2272     414     248      35    4910

  Fragments arranged by size: 

                4910    2272    1163     503     414     248      35
 
BanII G_rGCy'C

Cuts at:   2432    3254    5687    5745    6559    7589    2432 
   Size:        822    2433      58     814    1030    4388

  Fragments arranged by size: 

                4388    2433    1030     822     814      58
 
BbeI G_GCGC'C

Cuts at:   4293    6979    7227    4293 
   Size:       2686     248    6611

  Fragments arranged by size: 

                6611    2686     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   2286    2573    2827    3874    4676    5017    2286 
   Size:        287     254    1047     802     341    6814

  Fragments arranged by size: 

                6814    1047     802     341     287     254
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   4399    4433    6245    6279    4399 
   Size:         34    1812      34    7665

  Fragments arranged by size: 

                7665    1812      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   7443    8499    9545    7443 
   Size:       1056    1046    7443

  Fragments arranged by size: 

                7443    1056    1046
 
BclI T'GATC_A

Cuts at:    995    1884    4916    7065     995 
   Size:        889    3032    2149    3475

  Fragments arranged by size: 

                3475    3032    2149     889
 
BfrBI ATG'CAT

Cuts at:   6251    6251 
   Size:       9545
 
BglII A'GATC_T

Cuts at:    660    1464    3681    7060    9097     660 
   Size:        804    2217    3379    2037    1108

  Fragments arranged by size: 

                3379    2217    2037    1108     804
 
BlpI GC'TnA_GC

Cuts at:    735    1561    6657     735 
   Size:        826    5096    3623

  Fragments arranged by size: 

                5096    3623     826
 
Bme1580I G_kGCm'C

Cuts at:   2415    3377    3634    4036    7170    7263    8387    2415 
   Size:        962     257     402    3134      93    1124    3573

  Fragments arranged by size: 

                3573    3134    1124     962     402     257      93
 
BmgBI CAC'GTC

Cuts at:   4275    4275 
   Size:       9545
 
BmrI ACTGGGnnnn_n'

Cuts at:   2392    4333    5173    6757    6838    7173    9317    2392 
   Size:       1941     840    1584      81     335    2144    2620

  Fragments arranged by size: 

                2620    2144    1941    1584     840     335      81
 
BmtI G_CTAG'C

Cuts at:    328    1301     328 
   Size:        973    8572
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3748    5289    5376    5706    6541    7912    3748 
   Size:       1541      87     330     835    1371    5381

  Fragments arranged by size: 

                5381    1541    1371     835     330      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    469     635    2843    2917    3622    3893    4492    4780    5195 
   Size:        166    2208      74     705     271     599     288     415

Cuts at:   5195    5302    5346    5494    5743    5785    6511     469 
   Size:        107      44     148     249      42     726    3503

  Fragments arranged by size: 

                3503    2208     726     705     599     415     288     271
                 249     166     148     107      74      44      42
 
Bpu10I CC'TnA_GC

Cuts at:    117     117 
   Size:       9545
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2765    6721    7600    8314    8612    2765 
   Size:       3956     879     714     298    3698

  Fragments arranged by size: 

                3956    3698     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4756    5364    9249    4756 
   Size:        608    3885    5052

  Fragments arranged by size: 

                5052    3885     608
 
BsaAI yAC'GTr

Cuts at:   6898    6898 
   Size:       9545
 
BsaBI GATnn'nnATC

Cuts at:      6     858    4501    7059    9096       6 
   Size:        852    3643    2558    2037     455

  Fragments arranged by size: 

                3643    2558    2037     852     455
 
BsaHI Gr'CG_yC

Cuts at:    112    4290    6976    7224    7926    9422     112 
   Size:       4178    2686     248     702    1496     235

  Fragments arranged by size: 

                4178    2686    1496     702     248     235
 
BsaWI w'CCGG_w

Cuts at:   4053    6734    7255    7933    8344    8491    8765    4053 
   Size:       2681     521     678     411     147     274    4833

  Fragments arranged by size: 

                4833    2681     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    128     158    4091    4121     128 
   Size:         30    3933      30    5552

  Fragments arranged by size: 

                5552    3933      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   2197    2200    3013    3961    4342    5239    6324    2197 
   Size:          3     813     948     381     897    1085    5418

  Fragments arranged by size: 

                5418    1085     948     897     813     381       3
 
BseYI C'CCAG_C

Cuts at:    122    2024    5300    5308    5744    6128    6970    7728    8393 
   Size:       1902    3276       8     436     384     842     758     665

Cuts at:   8393    8801     122 
   Size:        408     866

  Fragments arranged by size: 

                3276    1902     866     842     758     665     436     408
                 384       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1990    2569    4349    4805    5083    5174    1990 
   Size:        579    1780     456     278      91    6361

  Fragments arranged by size: 

                6361    1780     579     456     278      91
 
BsiEI CG_ry'CG

Cuts at:    104    7087    7133    8363     104 
   Size:       6983      46    1230    1286

  Fragments arranged by size: 

                6983    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    755    1028    2240    2432    3377    3540    4286    5687    6559 
   Size:        273    1212     192     945     163     746    1401     872

Cuts at:   6559    7337    7527    8387     755 
   Size:        778     190     860    1913

  Fragments arranged by size: 

                1913    1401    1212     945     872     860     778     746
                 273     192     190     163
 
BsmI GAATG_Cn'

Cuts at:   3407    5450    3407 
   Size:       2043    7502
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1179    2247    5527    1179 
   Size:       1068    3280    5197

  Fragments arranged by size: 

                5197    3280    1068
 
Bsp1286I G_dGCh'C

Cuts at:    755    1028    2240    2415    2432    3254    3377    3540    3634 
   Size:        273    1212     175      17     822     123     163      94

Cuts at:   3634    4036    4286    5687    5745    6559    7170    7263    7337 
   Size:        402     250    1401      58     814     611      93      74

Cuts at:   7337    7527    7589    8387     755 
   Size:        190      62     798    1913

  Fragments arranged by size: 

                1913    1401    1212     822     814     798     611     402
                 273     250     190     175     163     123      94      93
                  74      62      58      17
 
BspEI T'CCGG_A

Cuts at:   4053    4053 
   Size:       9545
 
BspHI T'CATG_A

Cuts at:   1622    7977    1622 
   Size:       6355    3190
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   5384    6595    6809    7111    7492    5384 
   Size:       1211     214     302     381    7437

  Fragments arranged by size: 

                7437    1211     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    7837    7891    9375    9540      50 
   Size:       7787      54    1484     165      55

  Fragments arranged by size: 

                7787    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    500    2810    5933    7457     500 
   Size:       2310    3123    1524    2588

  Fragments arranged by size: 

                3123    2588    2310    1524
 
BsrFI r'CCGG_y

Cuts at:   5971    7543    7724    5971 
   Size:       1572     181    7792

  Fragments arranged by size: 

                7792    1572     181
 
BssHII G'CGCG_C

Cuts at:   4839    4841    5246    4839 
   Size:          2     405    9138

  Fragments arranged by size: 

                9138     405       2
 
BssSI C'ACGA_G

Cuts at:    523    7816    8524     523 
   Size:       7293     708    1544

  Fragments arranged by size: 

                7293    1544     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1155    3660    6922    1155 
   Size:       2505    3262    3778

  Fragments arranged by size: 

                3778    3262    2505
 
BstBI TT'CG_AA

Cuts at:   7906    7906 
   Size:       9545
 
BstEII G'GTnAC_C

Cuts at:   5268    5268 
   Size:       9545
 
BstXI CCAn_nnnn'nTGG

Cuts at:   5355    5355 
   Size:       9545
 
BstYI r'GATC_y

Cuts at:      1     660    1464    2133    2393    3239    3339    3681    5166 
   Size:        659     804     669     260     846     100     342    1485

Cuts at:   5166    6570    7037    7060    7395    7641    7947    7959    8045 
   Size:       1404     467      23     335     246     306      12      86

Cuts at:   8045    9097       1 
   Size:       1052     449

  Fragments arranged by size: 

                1485    1404    1052     846     804     669     659     467
                 449     342     335     306     260     246     100      86
                  23      12
 
BstZ17I GTA'TAC

Cuts at:   5123    5123 
   Size:       9545
 
Bsu36I CC'TnA_GG

Cuts at:    206    6341     206 
   Size:       6135    3410
 
BtgI C'CryG_G

Cuts at:    926    2453    3278    4799    5813    6272     926 
   Size:       1527     825    1521    1014     459    4199

  Fragments arranged by size: 

                4199    1527    1521    1014     825     459
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    807    6237    7495    7676    7839     807 
   Size:       5430    1258     181     163    2513

  Fragments arranged by size: 

                5430    2513    1258     181     163
 
BtsI GCAGTG_nn'

Cuts at:    648    2351    2763    3558    4565    5189    6788     648 
   Size:       1703     412     795    1007     624    1599    3405

  Fragments arranged by size: 

                3405    1703    1599    1007     795     624     412
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1883    1918    1883 
   Size:         35    9510
 
DraI TTT'AAA

Cuts at:   2261    3332    3561    4647    4736    6544    2261 
   Size:       1071     229    1086      89    1808    5262

  Fragments arranged by size: 

                5262    1808    1086    1071     229      89
 
DraIII CAC_nnn'GTG

Cuts at:   3535    4327    5982    3535 
   Size:        792    1655    7098

  Fragments arranged by size: 

                7098    1655     792
 
DrdI GACnn_nn'nnGTC

Cuts at:   7251    8595    7251 
   Size:       1344    8201
 
EaeI y'GGCC_r

Cuts at:    215    1598    2450    5054    5291    5973    6269    7130    7304 
   Size:       1383     852    2604     237     682     296     861     174

Cuts at:   7304    7695    7722    9067     215 
   Size:        391      27    1345     693

  Fragments arranged by size: 

                2604    1383    1345     861     852     693     682     391
                 296     237     174      27
 
EagI C'GGCC_G

Cuts at:   7130    7130 
   Size:       9545
 
EarI CTCTTCn'nnn_

Cuts at:    260    1148    1307    1859    2420    3340    4220    4742    5675 
   Size:        888     159     552     561     920     880     522     933

Cuts at:   5675    6203    7568    7778     260 
   Size:        528    1365     210    2027

  Fragments arranged by size: 

                2027    1365     933     920     888     880     561     552
                 528     522     210     159
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3973    6292    7753    8495    8641    9355    3973 
   Size:       2319    1461     742     146     714    4163

  Fragments arranged by size: 

                4163    2319    1461     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2430    5685    6557    2430 
   Size:       3255     872    5418

  Fragments arranged by size: 

                5418    3255     872
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    469     635    1229    1291    1734    2221    2280    2843    2917 
   Size:        166     594      62     443     487      59     563      74

Cuts at:   2917    2993    3157    3622    3893    4492    4780    5195    5302 
   Size:         76     164     465     271     599     288     415     107

Cuts at:   5302    5346    5494    5743    5785    6511    7370    7802    8155 
   Size:         44     148     249      42     726     859     432     353

Cuts at:   8155    8817     469 
   Size:        662    1197

  Fragments arranged by size: 

                1197     859     726     662     599     594     563     487
                 465     443     432     415     353     288     271     249
                 166     164     148     107      76      74      62      59
                  44      42
 
EcoO109I rG'GnC_Cy

Cuts at:   2937    5727    5846    6684    2937 
   Size:       2790     119     838    5798

  Fragments arranged by size: 

                5798    2790     838     119
 
EcoRI G'AATT_C

Cuts at:    945    1592    6549     945 
   Size:        647    4957    3941

  Fragments arranged by size: 

                4957    3941     647
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    289    1257    1270    1296    2053    6090    7564     289 
   Size:        968      13      26     757    4037    1474    2270

  Fragments arranged by size: 

                4037    2270    1474     968     757      26      13
 
FspI TGC'GCA

Cuts at:   7326    7326 
   Size:       9545
 
HaeII r_GCGC'y

Cuts at:    790    4293    4966    5290    6979    7227    8457    9228     790 
   Size:       3503     673     324    1689     248    1230     771    1107

  Fragments arranged by size: 

                3503    1689    1230    1107     771     673     324     248
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    128     160     793     825     888     920    1758    1790    1876 
   Size:         32     633      32      63      32     838      32      86

Cuts at:   1876    1908    1936    1968    2173    2205    2769    2801    2961 
   Size:         32      28      32     205      32     564      32     160

Cuts at:   2961    2993    3673    3705    4091    4123    4707    4739    6156 
   Size:         32     680      32     386      32     584      32    1417

Cuts at:   6156    6188    7387    7417    7419    7449     128 
   Size:         32    1199      30       2      30    2224

  Fragments arranged by size: 

                2224    1417    1199     838     680     633     584     564
                 386     205     160      86      63      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      30      30      28       2
 
HincII GTy'rAC

Cuts at:   3183    5909    6584    3183 
   Size:       2726     675    6144

  Fragments arranged by size: 

                6144    2726     675
 
HindIII A'AGCT_T

Cuts at:   2323    3484    4491    4856    5131    2323 
   Size:       1161    1007     365     275    6737

  Fragments arranged by size: 

                6737    1161    1007     365     275
 
KasI G'GCGC_C

Cuts at:   4289    6975    7223    4289 
   Size:       2686     248    6611

  Fragments arranged by size: 

                6611    2686     248
 
KpnI G_GTAC'C

Cuts at:   6565    6565 
   Size:       9545
 
MluI A'CGCG_T

Cuts at:   8978    8978 
   Size:       9545
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    356    3684    5754    8304    8488     356 
   Size:       3328    2070    2550     184    1413

  Fragments arranged by size: 

                3328    2550    2070    1413     184
 
MscI TGG'CCA

Cuts at:   1600    5056    5293    6271    7306    1600 
   Size:       3456     237     978    1035    3839

  Fragments arranged by size: 

                3839    3456    1035     978     237
 
MslI CAynn'nnrTG

Cuts at:    441    2668    3259    4013    4912    5194    7534    7661     441 
   Size:       2227     591     754     899     282    2340     127    2325

  Fragments arranged by size: 

                2340    2325    2227     899     754     591     282     127
            
 
MspA1I CmG'CkG

Cuts at:    129    1846    3259    6186    6656    6970    7330    8114    8359 
   Size:       1717    1413    2927     470     314     360     784     245

Cuts at:   8359     129 
   Size:       1315

  Fragments arranged by size: 

                2927    1717    1413    1315     784     470     360     314
                 245
 
NaeI GCC'GGC

Cuts at:   5973    7726    5973 
   Size:       1753    7792
 
NarI GG'CG_CC

Cuts at:   4290    6976    7224    4290 
   Size:       2686     248    6611

  Fragments arranged by size: 

                6611    2686     248
 
NcoI C'CATG_G

Cuts at:    926    3278    4799    5813    6272     926 
   Size:       2352    1521    1014     459    4199

  Fragments arranged by size: 

                4199    2352    1521    1014     459
 
NgoMIV G'CCGG_C

Cuts at:   5971    7724    5971 
   Size:       1753    7792
 
NheI G'CTAG_C

Cuts at:    324    1297     324 
   Size:        973    8572
 
NsiI A_TGCA'T

Cuts at:   6253    6253 
   Size:       9545
 
NspI r_CATG'y

Cuts at:   1158    6598    7098    8701    1158 
   Size:       5440     500    1603    2002

  Fragments arranged by size: 

                5440    2002    1603     500
 
PacI TTA_AT'TAA

Cuts at:   1859    1859 
   Size:       9545
 
PciI A'CATG_T

Cuts at:   8697    8697 
   Size:       9545
 
PflMI CCAn_nnn'nTGG

Cuts at:   1293    3916    1293 
   Size:       2623    6922
 
PfoI T'CCnGG_A

Cuts at:   3737    4277    5002    5720    6218    3737 
   Size:        540     725     718     498    7064

  Fragments arranged by size: 

                7064     725     718     540     498
 
PmeI GTTT'AAAC

Cuts at:   6544    6544 
   Size:       9545
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3937    3969    7991    8023    3937 
   Size:         32    4022      32    5459

  Fragments arranged by size: 

                5459    4022      32      32
 
PsiI TTA'TAA

Cuts at:   3364    6139    3364 
   Size:       2775    6770
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    627     659    2891    2923    6088    6120     627 
   Size:         32    2232      32    3165      32    4052

  Fragments arranged by size: 

                4052    3165    2232      32      32      32
 
PstI C_TGCA'G

Cuts at:    801    1034    2346    2758    2777    3752    4991    5402    6592 
   Size:        233    1312     412      19     975    1239     411    1190

Cuts at:   6592    7277     801 
   Size:        685    3069

  Fragments arranged by size: 

                3069    1312    1239    1190     975     685     412     411
                 233      19
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       9545
 
PvuII CAG'CTG

Cuts at:    129    3259    6186    6970    7330     129 
   Size:       3130    2927     784     360    2344

  Fragments arranged by size: 

                3130    2927    2344     784     360
 
RsrII CG'GwC_CG

Cuts at:   7740    7740 
   Size:       9545
 
SacI G_AGCT'C

Cuts at:   2432    5687    6559    2432 
   Size:       3255     872    5418

  Fragments arranged by size: 

                5418    3255     872
 
SalI G'TCGA_C

Cuts at:   6582    6582 
   Size:       9545
 
SapI GCTCTTCn'nnn_

Cuts at:   2420    5675    7568    7778    2420 
   Size:       3255    1893     210    4187

  Fragments arranged by size: 

                4187    3255    1893     210
 
SbfI CC_TGCA'GG

Cuts at:   6592    6592 
   Size:       9545
 
ScaI AGT'ACT

Cuts at:   3170    4513    9062    3170 
   Size:       1343    4549    3653

  Fragments arranged by size: 

                4549    3653    1343
 
SexAI A'CCwGG_T

Cuts at:   6008    6008 
   Size:       9545
 
SfcI C'TryA_G

Cuts at:     33     797    1030    2342    2754    2773    3058    3748    4202 
   Size:        764     233    1312     412      19     285     690     454

Cuts at:   4202    4597    4987    5398    6588    7273    8241    8432      33 
   Size:        395     390     411    1190     685     968     191    1146

  Fragments arranged by size: 

                1312    1190    1146     968     764     690     685     454
                 412     411     395     390     285     233     191      19
            
 
SfoI GGC'GCC

Cuts at:   4291    6977    7225    4291 
   Size:       2686     248    6611

  Fragments arranged by size: 

                6611    2686     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9545
 
SmaI CCC'GGG

Cuts at:    728    6567     728 
   Size:       5839    3706
 
SmlI C'TyrA_G

Cuts at:    427    1834    2780    6700    7615    8329    8591     427 
   Size:       1407     946    3920     915     714     262    1381

  Fragments arranged by size: 

                3920    1407    1381     946     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67    5485      67 
   Size:       5418    4127
 
SphI G_CATG'C

Cuts at:   6598    7098    6598 
   Size:        500    9045
 
SspI AAT'ATT

Cuts at:    536    4049     536 
   Size:       3513    6032
 
StuI AGG'CCT

Cuts at:   4742    4742 
   Size:       9545
 
StyI C'CwwG_G

Cuts at:    335     926    1053    3278    3626    4799    5387    5813    6272 
   Size:        591     127    2225     348    1173     588     426     459

Cuts at:   6272    6679    7033     335 
   Size:        407     354    2847

  Fragments arranged by size: 

                2847    2225    1173     591     588     459     426     407
                 354     348     127
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2167    4315    7128    7261    7720    7929    2167 
   Size:       2148    2813     133     459     209    3783

  Fragments arranged by size: 

                3783    2813    2148     459     209     133
 
TatI w'GTAC_w

Cuts at:    253    3168    4511    9060     253 
   Size:       2915    1343    4549     738

  Fragments arranged by size: 

                4549    2915    1343     738
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5552    5584    9062    9094    5552 
   Size:         32    3478      32    6003

  Fragments arranged by size: 

                6003    3478      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   7342    7342 
   Size:       9545
 
XbaI T'CTAG_A

Cuts at:    172    2676    3351    3684    6576     172 
   Size:       2504     675     333    2892    3141

  Fragments arranged by size: 

                3141    2892    2504     675     333
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2405    2941    7040    2405 
   Size:        536    4099    4910

  Fragments arranged by size: 

                4910    4099     536
 
XmaI C'CCGG_G

Cuts at:    726    6565     726 
   Size:       5839    3706
 
XmnI GAAnn'nnTTC

Cuts at:   1379    2659    3473    3692    4547    5450    6425    1379 
   Size:       1280     814     219     855     903     975    4499

  Fragments arranged by size: 

                4499    1280     975     903     855     814     219
 
ZraI GAC'GTC

Cuts at:    113     113 
   Size:       9545
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PpiI     PsiI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AgeI     AhdI     ApaI     AscI    AvrII     BaeI    BbvCI
     BglI    BsiWI    BsrGI     ClaI    EcoNI    EcoRV     FseI    FspAI
     HpaI     MfeI     NdeI     NotI     NruI     PasI     PmlI    PpuMI
    PshAI   PspOMI    PspXI    SacII    SanDI     SfiI    SgrAI    SnaBI
     SrfI     SwaI     XhoI
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