Restriction map for
pF1KB9471
(Circular) (Six-base) MAPSORT of: pF1KB9471.seq Check: 7534 from: 1 to: 6410
pF1KB9471 6410 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 369 1428 2015 2727 369
Size: 1059 587 712 4052
Fragments arranged by size:
4052 1059 712 587
AccI GT'mk_AC
Cuts at: 1976 3448 1976
Size: 1472 4938
Acc65I G'GTAC_C
Cuts at: 1674 3426 1674
Size: 1752 4658
AclI AA'CG_TT
Cuts at: 6250 6250
Size: 6410
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 1313 1592 2231 2560 3343 4235 4667 5020
Size: 691 279 639 329 783 892 432 353
Cuts at: 5020 5682 622
Size: 662 1350
Fragments arranged by size:
1350 892 783 691 662 639 432 353
329 279
AfeI AGC'GCT
Cuts at: 1109 3314 1109
Size: 2205 4205
AflIII A'CryG_T
Cuts at: 3762 5562 5843 3762
Size: 1800 281 4329
Fragments arranged by size:
4329 1800 281
AleI CACnn'nnGTG
Cuts at: 2254 2254
Size: 6410
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 916 948 916
Size: 32 6378
AlwNI CAG_nnn'CTG
Cuts at: 467 741 1335 1696 2475 2596 2679 3372 3715
Size: 274 594 361 779 121 83 693 343
Cuts at: 3715 5153 467
Size: 1438 1724
Fragments arranged by size:
1724 1438 779 693 594 361 343 274
121 83
ApaI G_GGCC'C
Cuts at: 430 625 808 430
Size: 195 183 6032
Fragments arranged by size:
6032 195 183
ApaLI G'TGCA_C
Cuts at: 5248 5248
Size: 6410
ApoI r'AATT_y
Cuts at: 702 3414 5763 6019 702
Size: 2712 2349 256 1093
Fragments arranged by size:
2712 2349 1093 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6410
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6410
AvaI C'yCGr_G
Cuts at: 247 1834 1924 3430 247
Size: 1587 90 1506 3227
Fragments arranged by size:
3227 1587 1506 90
BamHI G'GATC_C
Cuts at: 3435 3435
Size: 6410
BanI G'GyrC_C
Cuts at: 693 1026 1198 1478 1674 2461 2853 2943 3426
Size: 333 172 280 196 787 392 90 483
Cuts at: 3426 3840 4088 4123 693
Size: 414 248 35 2980
Fragments arranged by size:
2980 787 483 414 392 333 280 248
196 172 90 35
BanII G_rGCy'C
Cuts at: 430 553 625 808 1931 2287 2900 2921 3424
Size: 123 72 183 1123 356 613 21 503
Cuts at: 3424 4454 430
Size: 1030 2386
Fragments arranged by size:
2386 1123 1030 613 503 356 183 123
72 21
BbeI G_GCGC'C
Cuts at: 1482 3844 4092 1482
Size: 2362 248 3800
Fragments arranged by size:
3800 2362 248
BbsI GAAGACnn'nnnn_
Cuts at: 786 1667 2423 786
Size: 881 756 4773
Fragments arranged by size:
4773 881 756
BbvCI CC'TCA_GC
Cuts at: 357 1318 2086 3316 357
Size: 961 768 1230 3451
Fragments arranged by size:
3451 1230 961 768
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1944 1978 1944
Size: 34 6376
BciVI GTATCCnnnnn_n'
Cuts at: 1177 2775 4308 5364 6410 1177
Size: 1598 1533 1056 1046 1177
Fragments arranged by size:
1598 1533 1177 1056 1046
BclI T'GATC_A
Cuts at: 3930 3930
Size: 6410
BglI GCCn_nnn'nGGC
Cuts at: 987 1522 2553 987
Size: 535 1031 4844
Fragments arranged by size:
4844 1031 535
BglII A'GATC_T
Cuts at: 3925 5962 3925
Size: 2037 4373
BlpI GC'TnA_GC
Cuts at: 1784 3522 1784
Size: 1738 4672
Bme1580I G_kGCm'C
Cuts at: 254 430 625 808 1957 2181 2328 3153 4035
Size: 176 195 183 1149 224 147 825 882
Cuts at: 4035 4128 5252 254
Size: 93 1124 1412
Fragments arranged by size:
1412 1149 1124 882 825 224 195 183
176 147 93
BmrI ACTGGGnnnn_n'
Cuts at: 1003 1684 2248 2697 3622 3703 4038 6182 1003
Size: 681 564 449 925 81 335 2144 1231
Fragments arranged by size:
2144 1231 925 681 564 449 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 372 1554 4777 372
Size: 1182 3223 2005
Fragments arranged by size:
3223 2005 1182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 401 581 694 1333 1760 2424 2914 100
Size: 301 180 113 639 427 664 490 3596
Fragments arranged by size:
3596 664 639 490 427 301 180 113
Bpu10I CC'TnA_GC
Cuts at: 347 357 1318 1638 1853 2086 3316 347
Size: 10 961 320 215 233 1230 3441
Fragments arranged by size:
3441 1230 961 320 233 215 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1832 3586 4465 5179 5477 1832
Size: 1754 879 714 298 2765
Fragments arranged by size:
2765 1754 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 6114 6114
Size: 6410
BsaAI yAC'GTr
Cuts at: 3763 3763
Size: 6410
BsaBI GATnn'nnATC
Cuts at: 6 3924 5961 6
Size: 3918 2037 455
Fragments arranged by size:
3918 2037 455
BsaHI Gr'CG_yC
Cuts at: 1479 3841 4089 4791 6287 1479
Size: 2362 248 702 1496 1602
Fragments arranged by size:
2362 1602 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2195 3065 3599 4120 4798 5209 5356 5630 2195
Size: 870 534 521 678 411 147 274 2975
Fragments arranged by size:
2975 870 678 534 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 226 256 257 287 554 584 2269 2299 226
Size: 30 1 30 267 30 1685 30 4337
Fragments arranged by size:
4337 1685 267 30 30 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 455 1414 2147 2519 2950 2953 2980 3394
Size: 205 959 733 372 431 3 27 414
Cuts at: 3394 250
Size: 3266
Fragments arranged by size:
3266 959 733 431 414 372 205 27
3
BseYI C'CCAG_C
Cuts at: 122 212 374 985 1177 1202 3835 4593 5258
Size: 90 162 611 192 25 2633 758 665
Cuts at: 5258 5666 122
Size: 408 866
Fragments arranged by size:
2633 866 758 665 611 408 192 162
90 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 2467 2476 2692 688
Size: 1779 9 216 4406
Fragments arranged by size:
4406 1779 216 9
BsiEI CG_ry'CG
Cuts at: 104 2751 3952 3998 5228 104
Size: 2647 1201 46 1230 1286
Fragments arranged by size:
2647 1286 1230 1201 46
BsiHKAI G_wGCw'C
Cuts at: 1423 1468 1656 1790 3424 4202 4392 5252 1423
Size: 45 188 134 1634 778 190 860 2581
Fragments arranged by size:
2581 1634 860 778 190 188 134 45
BsmI GAATG_Cn'
Cuts at: 766 1554 766
Size: 788 5622
Bsp1286I G_dGCh'C
Cuts at: 254 430 553 625 808 1423 1468 1656 1790
Size: 176 123 72 183 615 45 188 134
Cuts at: 1790 1931 1957 2181 2287 2328 2900 2921 3153
Size: 141 26 224 106 41 572 21 232
Cuts at: 3153 3424 4035 4128 4202 4392 4454 5252 254
Size: 271 611 93 74 190 62 798 1412
Fragments arranged by size:
1412 798 615 611 572 271 232 224
190 188 183 176 141 134 123 106
93 74 72 62 45 41 26 21
BspEI T'CCGG_A
Cuts at: 2195 3065 2195
Size: 870 5540
BspHI T'CATG_A
Cuts at: 4842 4842
Size: 6410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 369 780 1428 2015 2429 2727 3460 3674 3976
Size: 411 648 587 414 298 733 214 302
Cuts at: 3976 4357 369
Size: 381 2422
Fragments arranged by size:
2422 733 648 587 414 411 381 302
298 214
BsrBI CCG'CTC
Cuts at: 50 351 915 1142 2046 3386 4702 4756 6240
Size: 301 564 227 904 1340 1316 54 1484
Cuts at: 6240 6405 50
Size: 165 55
Fragments arranged by size:
1484 1340 1316 904 564 301 227 165
55 54
BsrDI GCAATG_nn'
Cuts at: 2639 2760 4322 2639
Size: 121 1562 4727
Fragments arranged by size:
4727 1562 121
BsrFI r'CCGG_y
Cuts at: 785 3097 4408 4589 785
Size: 2312 1311 181 2606
Fragments arranged by size:
2606 2312 1311 181
BssSI C'ACGA_G
Cuts at: 253 1655 3073 4681 5389 253
Size: 1402 1418 1608 708 1274
Fragments arranged by size:
1608 1418 1402 1274 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1335 2490 3372 3787 1335
Size: 1155 882 415 3958
Fragments arranged by size:
3958 1155 882 415
BstBI TT'CG_AA
Cuts at: 4771 4771
Size: 6410
BstXI CCAn_nnnn'nTGG
Cuts at: 970 1185 2697 970
Size: 215 1512 4683
Fragments arranged by size:
4683 1512 215
BstYI r'GATC_y
Cuts at: 1 1124 2340 2951 3435 3902 3925 4260 4506
Size: 1123 1216 611 484 467 23 335 246
Cuts at: 4506 4812 4824 4910 5962 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1216 1123 1052 611 484 467 449 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 593 1115 593
Size: 522 5888
BtgI C'CryG_G
Cuts at: 1388 2269 2371 2696 1388
Size: 881 102 325 5102
Fragments arranged by size:
5102 881 325 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1947 2167 2804 4360 4541 4704 1947
Size: 220 637 1556 181 163 3653
Fragments arranged by size:
3653 1556 637 220 181 163
BtsI GCAGTG_nn'
Cuts at: 739 1421 1540 1569 3273 3653 739
Size: 682 119 29 1704 380 3496
Fragments arranged by size:
3496 1704 682 380 119 29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 806 841 806
Size: 35 6375
DraI TTT'AAA
Cuts at: 3409 3409
Size: 6410
DraIII CAC_nnn'GTG
Cuts at: 780 780
Size: 6410
DrdI GACnn_nn'nnGTC
Cuts at: 1870 3138 4116 5460 1870
Size: 1268 978 1344 2820
Fragments arranged by size:
2820 1344 1268 978
EaeI y'GGCC_r
Cuts at: 771 1365 2612 2748 3044 3102 3995 4169 4560
Size: 594 1247 136 296 58 893 174 391
Cuts at: 4560 4587 5932 771
Size: 27 1345 1249
Fragments arranged by size:
1345 1249 1247 893 594 391 296 174
136 58 27
EagI C'GGCC_G
Cuts at: 2748 3995 2748
Size: 1247 5163
EarI CTCTTCn'nnn_
Cuts at: 163 1568 2804 2862 2864 2879 2969 3253 3274
Size: 1405 1236 58 2 15 90 284 21
Cuts at: 3274 4433 4643 163
Size: 1159 210 1930
Fragments arranged by size:
1930 1405 1236 1159 284 210 90 58
21 15 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1466 4618 5360 5506 6220 1466
Size: 3152 742 146 714 1656
Fragments arranged by size:
3152 1656 742 714 146
EcoICRI GAG'CTC
Cuts at: 3422 3422
Size: 6410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 401 581 622 694 1313 1333 1592 1760
Size: 301 180 41 72 619 20 259 168
Cuts at: 1760 2231 2424 2560 2914 3343 4235 4667 5020
Size: 471 193 136 354 429 892 432 353
Cuts at: 5020 5682 100
Size: 662 828
Fragments arranged by size:
892 828 662 619 471 432 429 354
353 301 259 193 180 168 136 72
41 20
EcoNI CCTnn'n_nnAGG
Cuts at: 1084 1156 2313 3376 1084
Size: 72 1157 1063 4118
Fragments arranged by size:
4118 1157 1063 72
EcoO109I rG'GnC_Cy
Cuts at: 426 621 804 1077 1765 2379 3369 3549 426
Size: 195 183 273 688 614 990 180 3287
Fragments arranged by size:
3287 990 688 614 273 195 183 180
EcoRI G'AATT_C
Cuts at: 702 3414 702
Size: 2712 3698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2373 2679 4429 2373
Size: 306 1750 4354
Fragments arranged by size:
4354 1750 306
FspI TGC'GCA
Cuts at: 1072 3272 4191 1072
Size: 2200 919 3291
Fragments arranged by size:
3291 2200 919
FspAI rTGC'GCAy
Cuts at: 3272 3272
Size: 6410
HaeII r_GCGC'y
Cuts at: 1111 1184 1482 3316 3844 4092 5322 6093 1111
Size: 73 298 1834 528 248 1230 771 1428
Fragments arranged by size:
1834 1428 1230 771 528 298 248 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 257 289 540 554 572 586 1830 1862 2023
Size: 32 251 14 18 14 1244 32 161
Cuts at: 2023 2055 2166 2198 2495 2527 2782 2814 3189
Size: 32 111 32 297 32 255 32 375
Cuts at: 3189 3221 4252 4282 4284 4314 257
Size: 32 1031 30 2 30 2353
Fragments arranged by size:
2353 1244 1031 375 297 255 251 161
111 32 32 32 32 32 32 32
30 30 18 14 14 2
HincII GTy'rAC
Cuts at: 3449 3449
Size: 6410
KasI G'GCGC_C
Cuts at: 1478 3840 4088 1478
Size: 2362 248 3800
Fragments arranged by size:
3800 2362 248
KpnI G_GTAC'C
Cuts at: 1678 3430 1678
Size: 1752 4658
MluI A'CGCG_T
Cuts at: 5843 5843
Size: 6410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1859 2006 3104 5169 5353 1859
Size: 147 1098 2065 184 2916
Fragments arranged by size:
2916 2065 1098 184 147
MscI TGG'CCA
Cuts at: 3104 4171 3104
Size: 1067 5343
MslI CAynn'nnrTG
Cuts at: 226 1690 2254 4399 4526 226
Size: 1464 564 2145 127 2110
Fragments arranged by size:
2145 2110 1464 564 127
MspA1I CmG'CkG
Cuts at: 467 630 672 762 985 1311 1914 2335 2571
Size: 163 42 90 223 326 603 421 236
Cuts at: 2571 3004 3340 3521 3835 4195 4979 5224 467
Size: 433 336 181 314 360 784 245 1653
Fragments arranged by size:
1653 784 603 433 421 360 336 326
314 245 236 223 181 163 90 42
NaeI GCC'GGC
Cuts at: 4591 4591
Size: 6410
NarI GG'CG_CC
Cuts at: 1479 3841 4089 1479
Size: 2362 248 3800
Fragments arranged by size:
3800 2362 248
NcoI C'CATG_G
Cuts at: 1388 2371 1388
Size: 983 5427
NdeI CA'TA_TG
Cuts at: 3275 3275
Size: 6410
NgoMIV G'CCGG_C
Cuts at: 4589 4589
Size: 6410
NspI r_CATG'y
Cuts at: 3463 3963 5566 3463
Size: 500 1603 4307
Fragments arranged by size:
4307 1603 500
PasI CC'CwG_GG
Cuts at: 1275 3088 1275
Size: 1813 4597
PciI A'CATG_T
Cuts at: 5562 5562
Size: 6410
PflMI CCAn_nnn'nTGG
Cuts at: 381 1023 2475 2571 2709 381
Size: 642 1452 96 138 4082
Fragments arranged by size:
4082 1452 642 138 96
PfoI T'CCnGG_A
Cuts at: 261 558 2513 261
Size: 297 1955 4158
Fragments arranged by size:
4158 1955 297
PmeI GTTT'AAAC
Cuts at: 3409 3409
Size: 6410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4856 4888 4856
Size: 32 6378
PpuMI rG'GwC_Cy
Cuts at: 1765 2379 1765
Size: 614 5796
PspOMI G'GGCC_C
Cuts at: 426 621 804 426
Size: 195 183 6032
Fragments arranged by size:
6032 195 183
PstI C_TGCA'G
Cuts at: 206 980 1088 1256 1547 2340 3312 3457 4142
Size: 774 108 168 291 793 972 145 685
Cuts at: 4142 206
Size: 2474
Fragments arranged by size:
2474 972 793 774 685 291 168 145
108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6410
PvuII CAG'CTG
Cuts at: 467 630 672 762 985 2335 3835 4195 467
Size: 163 42 90 223 1350 1500 360 2682
Fragments arranged by size:
2682 1500 1350 360 223 163 90 42
RsrII CG'GwC_CG
Cuts at: 4605 4605
Size: 6410
SacI G_AGCT'C
Cuts at: 3424 3424
Size: 6410
SalI G'TCGA_C
Cuts at: 3447 3447
Size: 6410
SapI GCTCTTCn'nnn_
Cuts at: 3253 3274 4433 4643 3253
Size: 21 1159 210 5020
Fragments arranged by size:
5020 1159 210 21
SbfI CC_TGCA'GG
Cuts at: 3457 3457
Size: 6410
ScaI AGT'ACT
Cuts at: 5927 5927
Size: 6410
SexAI A'CCwGG_T
Cuts at: 776 776
Size: 6410
SfcI C'TryA_G
Cuts at: 33 202 531 976 1084 1252 1543 2336 3028
Size: 169 329 445 108 168 291 793 692
Cuts at: 3028 3308 3453 4138 5106 5297 33
Size: 280 145 685 968 191 1146
Fragments arranged by size:
1146 968 793 692 685 445 329 291
280 191 169 168 145 108
SfoI GGC'GCC
Cuts at: 1480 3842 4090 1480
Size: 2362 248 3800
Fragments arranged by size:
3800 2362 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6410
SmaI CCC'GGG
Cuts at: 1836 3432 1836
Size: 1596 4814
SmlI C'TyrA_G
Cuts at: 1847 3565 4480 5194 5456 1847
Size: 1718 915 714 262 2801
Fragments arranged by size:
2801 1718 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6410
SphI G_CATG'C
Cuts at: 3463 3963 3463
Size: 500 5910
StuI AGG'CCT
Cuts at: 3109 3109
Size: 6410
StyI C'CwwG_G
Cuts at: 128 182 542 1388 1946 2371 2848 3121 3544
Size: 54 360 846 558 425 477 273 423
Cuts at: 3544 3898 128
Size: 354 2640
Fragments arranged by size:
2640 846 558 477 425 423 360 354
273 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1216 1374 3993 4126 4585 4794 1216
Size: 158 2619 133 459 209 2832
Fragments arranged by size:
2832 2619 459 209 158 133
TatI w'GTAC_w
Cuts at: 5925 5925
Size: 6410
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 508 540 2190 2222 5927 5959 508
Size: 32 1650 32 3705 32 959
Fragments arranged by size:
3705 1650 959 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4207 4207
Size: 6410
XbaI T'CTAG_A
Cuts at: 3441 3441
Size: 6410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 408 1614 2472 3905 408
Size: 1206 858 1433 2913
Fragments arranged by size:
2913 1433 1206 858
XmaI C'CCGG_G
Cuts at: 1834 3430 1834
Size: 1596 4814
XmnI GAAnn'nnTTC
Cuts at: 706 2626 2659 3217 706
Size: 1920 33 558 3899
Fragments arranged by size:
3899 1920 558 33
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI EcoRV FseI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI