Restriction map for
pF1KB9472
(Circular) (Six-base) MAPSORT of: pF1KB9472.seq Check: 935 from: 1 to: 4625
pF1KB9472 4625 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 213 360 775 213
Size: 147 415 4063
Fragments arranged by size:
4063 415 147
AccI GT'mk_AC
Cuts at: 1663 1663
Size: 4625
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 751 970 2450 2882 3235 3897 379
Size: 372 219 1480 432 353 662 1107
Fragments arranged by size:
1480 1107 662 432 372 353 219
AfeI AGC'GCT
Cuts at: 1425 1425
Size: 4625
AflIII A'CryG_T
Cuts at: 398 1037 1578 1977 3777 4058 398
Size: 639 541 399 1800 281 965
Fragments arranged by size:
1800 965 639 541 399 281
AhdI GACnn_n'nnGTC
Cuts at: 1041 1041
Size: 4625
AleI CACnn'nnGTG
Cuts at: 877 877
Size: 4625
AlwNI CAG_nnn'CTG
Cuts at: 326 357 948 1504 1930 3368 326
Size: 31 591 556 426 1438 1583
Fragments arranged by size:
1583 1438 591 556 426 31
ApaI G_GGCC'C
Cuts at: 494 1492 494
Size: 998 3627
ApaLI G'TGCA_C
Cuts at: 1323 3463 1323
Size: 2140 2485
ApoI r'AATT_y
Cuts at: 1629 3978 4234 1629
Size: 2349 256 2020
Fragments arranged by size:
2349 2020 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 182 1645 182
Size: 1463 3162
BamHI G'GATC_C
Cuts at: 1650 1650
Size: 4625
BanI G'GyrC_C
Cuts at: 575 635 790 967 1126 1282 1355 1641 2055
Size: 60 155 177 159 156 73 286 414
Cuts at: 2055 2303 2338 575
Size: 248 35 2862
Fragments arranged by size:
2862 414 286 248 177 159 156 155
73 60 35
BanII G_rGCy'C
Cuts at: 121 494 704 1492 1639 2669 121
Size: 373 210 788 147 1030 2077
Fragments arranged by size:
2077 1030 788 373 210 147
BbeI G_GCGC'C
Cuts at: 579 794 971 1130 2059 2307 579
Size: 215 177 159 929 248 2897
Fragments arranged by size:
2897 929 248 215 177 159
BbsI GAAGACnn'nnnn_
Cuts at: 1263 1263
Size: 4625
BbvCI CC'TCA_GC
Cuts at: 1420 1420
Size: 4625
BciVI GTATCCnnnnn_n'
Cuts at: 2523 3579 4625 2523
Size: 1056 1046 2523
Fragments arranged by size:
2523 1056 1046
BclI T'GATC_A
Cuts at: 2145 2145
Size: 4625
BglI GCCn_nnn'nGGC
Cuts at: 354 354
Size: 4625
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 698 1737 698
Size: 1039 3586
Bme1580I G_kGCm'C
Cuts at: 262 494 543 638 1092 1285 1327 1358 1492
Size: 232 49 95 454 193 42 31 134
Cuts at: 1492 2250 2343 3467 262
Size: 758 93 1124 1420
Fragments arranged by size:
1420 1124 758 454 232 193 134 95
93 49 42 31
BmgBI CAC'GTC
Cuts at: 899 927 1038 899
Size: 28 111 4486
Fragments arranged by size:
4486 111 28
BmrI ACTGGGnnnn_n'
Cuts at: 256 1492 1495 1837 1918 2253 4397 256
Size: 1236 3 342 81 335 2144 484
Fragments arranged by size:
2144 1236 484 342 335 81 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 348
Size: 4625
Bpu10I CC'TnA_GC
Cuts at: 430 1420 430
Size: 990 3635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1801 2680 3394 3692 601
Size: 1200 879 714 298 1534
Fragments arranged by size:
1534 1200 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1466 4329 1466
Size: 2863 1762
BsaAI yAC'GTr
Cuts at: 399 656 1581 1978 399
Size: 257 925 397 3046
Fragments arranged by size:
3046 925 397 257
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 576 791 968 1127 2056 2304 3006 4502
Size: 464 215 177 159 929 248 702 1496
Cuts at: 4502 112
Size: 235
Fragments arranged by size:
1496 929 702 464 248 235 215 177
159
BsaWI w'CCGG_w
Cuts at: 1814 2335 3013 3424 3571 3845 1814
Size: 521 678 411 147 274 2594
Fragments arranged by size:
2594 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1444 1474 1444
Size: 30 4595
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 492 1200 1465 492
Size: 708 265 3652
Fragments arranged by size:
3652 708 265
BseYI C'CCAG_C
Cuts at: 153 234 1257 1475 2050 2808 3473 3881 153
Size: 81 1023 218 575 758 665 408 897
Fragments arranged by size:
1023 897 758 665 575 408 218 81
BsiEI CG_ry'CG
Cuts at: 104 173 547 745 1383 2167 2213 3443 104
Size: 69 374 198 638 784 46 1230 1286
Fragments arranged by size:
1286 1230 784 638 374 198 69 46
BsiHKAI G_wGCw'C
Cuts at: 851 1327 1639 2417 2607 3467 851
Size: 476 312 778 190 860 2009
Fragments arranged by size:
2009 860 778 476 312 190
Bsp1286I G_dGCh'C
Cuts at: 121 262 494 543 638 704 851 1092 1285
Size: 141 232 49 95 66 147 241 193
Cuts at: 1285 1327 1358 1492 1639 2250 2343 2417 2607
Size: 42 31 134 147 611 93 74 190
Cuts at: 2607 2669 3467 121
Size: 62 798 1279
Fragments arranged by size:
1279 798 611 241 232 193 190 147
147 141 134 95 93 74 66 62
49 42 31
BspHI T'CATG_A
Cuts at: 3057 3057
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 213 360 746 775 1675 1889 2191 2572 213
Size: 147 386 29 900 214 302 381 2266
Fragments arranged by size:
2266 900 386 381 302 214 147 29
BsrBI CCG'CTC
Cuts at: 50 378 2917 2971 4455 4620 50
Size: 328 2539 54 1484 165 55
Fragments arranged by size:
2539 1484 328 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2537 2537
Size: 4625
BsrFI r'CCGG_y
Cuts at: 168 388 933 1261 2623 2804 168
Size: 220 545 328 1362 181 1989
Fragments arranged by size:
1989 1362 545 328 220 181
BsrGI T'GTAC_A
Cuts at: 1112 1112
Size: 4625
BssSI C'ACGA_G
Cuts at: 2896 3604 2896
Size: 708 3917
BstAPI GCAn_nnn'nTGC
Cuts at: 2002 2002
Size: 4625
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 999 1137 999
Size: 138 4487
BstYI r'GATC_y
Cuts at: 1 1650 2117 2140 2475 2721 3027 3039 3125
Size: 1649 467 23 335 246 306 12 86
Cuts at: 3125 4177 1
Size: 1052 449
Fragments arranged by size:
1649 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 217 596 1028 1136 1234 1241 217
Size: 379 432 108 98 7 3601
Fragments arranged by size:
3601 432 379 108 98 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 262 541 1868 262
Size: 279 1327 3019
Fragments arranged by size:
3019 1327 279
ClaI AT'CG_AT
Cuts at: 995 995
Size: 4625
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DraIII CAC_nnn'GTG
Cuts at: 867 867
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 211 2331 3675 211
Size: 2120 1344 1161
Fragments arranged by size:
2120 1344 1161
EaeI y'GGCC_r
Cuts at: 170 306 626 742 1005 1220 1412 2210 2384
Size: 136 320 116 263 215 192 798 174
Cuts at: 2384 2775 2802 4147 170
Size: 391 27 1345 648
Fragments arranged by size:
1345 798 648 391 320 263 215 192
174 136 116 27
EagI C'GGCC_G
Cuts at: 170 742 2210 170
Size: 572 1468 2585
Fragments arranged by size:
2585 1468 572
EarI CTCTTCn'nnn_
Cuts at: 2648 2858 2648
Size: 210 4415
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 217 2833 3575 3721 4435 217
Size: 2616 742 146 714 407
Fragments arranged by size:
2616 742 714 407 146
EcoICRI GAG'CTC
Cuts at: 1637 1637
Size: 4625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 379 751 970 2450 2882 3235 3897 348
Size: 31 372 219 1480 432 353 662 1076
Fragments arranged by size:
1480 1076 662 432 372 353 219 31
EcoO109I rG'GnC_Cy
Cuts at: 158 187 273 490 519 1398 1764 158
Size: 29 86 217 29 879 366 3019
Fragments arranged by size:
3019 879 366 217 86 29 29
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 4625
FspI TGC'GCA
Cuts at: 919 2406 919
Size: 1487 3138
HaeII r_GCGC'y
Cuts at: 579 794 971 1130 1160 1268 1427 2059 2307
Size: 215 177 159 30 108 159 632 248
Cuts at: 2307 3537 4308 579
Size: 1230 771 896
Fragments arranged by size:
1230 896 771 632 248 215 177 159
159 108 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 1027 1059 1390 1422 1444 1476 2467
Size: 32 508 32 331 32 22 32 991
Cuts at: 2467 2497 2499 2529 487
Size: 30 2 30 2583
Fragments arranged by size:
2583 991 508 331 32 32 32 32
30 30 22 2
HincII GTy'rAC
Cuts at: 438 1664 438
Size: 1226 3399
KasI G'GCGC_C
Cuts at: 575 790 967 1126 2055 2303 575
Size: 215 177 159 929 248 2897
Fragments arranged by size:
2897 929 248 215 177 159
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3384 3568 3384
Size: 184 4441
MscI TGG'CCA
Cuts at: 628 2386 628
Size: 1758 2867
MslI CAynn'nnrTG
Cuts at: 658 877 997 2614 2741 658
Size: 219 120 1617 127 2542
Fragments arranged by size:
2542 1617 219 127 120
MspA1I CmG'CkG
Cuts at: 147 285 323 632 697 755 828 1388 1736
Size: 138 38 309 65 58 73 560 348
Cuts at: 1736 2050 2410 3194 3439 147
Size: 314 360 784 245 1333
Fragments arranged by size:
1333 784 560 360 348 314 309 245
138 73 65 58 38
NaeI GCC'GGC
Cuts at: 390 1263 2806 390
Size: 873 1543 2209
Fragments arranged by size:
2209 1543 873
NarI GG'CG_CC
Cuts at: 576 791 968 1127 2056 2304 576
Size: 215 177 159 929 248 2897
Fragments arranged by size:
2897 929 248 215 177 159
NcoI C'CATG_G
Cuts at: 1136 1136
Size: 4625
NgoMIV G'CCGG_C
Cuts at: 388 1261 2804 388
Size: 873 1543 2209
Fragments arranged by size:
2209 1543 873
NspI r_CATG'y
Cuts at: 1113 1678 2178 3781 1113
Size: 565 500 1603 1957
Fragments arranged by size:
1957 1603 565 500
PasI CC'CwG_GG
Cuts at: 472 472
Size: 4625
PciI A'CATG_T
Cuts at: 3777 3777
Size: 4625
PflMI CCAn_nnn'nTGG
Cuts at: 1504 1504
Size: 4625
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PmlI CAC'GTG
Cuts at: 656 1581 656
Size: 925 3700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3071 3103 3071
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 273 519 1398 273
Size: 246 879 3500
Fragments arranged by size:
3500 879 246
PspOMI G'GGCC_C
Cuts at: 490 1488 490
Size: 998 3627
PstI C_TGCA'G
Cuts at: 257 1672 2357 257
Size: 1415 685 2525
Fragments arranged by size:
2525 1415 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 2050 2410 2050
Size: 360 4265
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 1639 1639
Size: 4625
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SanDI GG'GwC_CC
Cuts at: 273 273
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 2648 2858 2648
Size: 210 4415
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 4142 4142
Size: 4625
SfcI C'TryA_G
Cuts at: 33 253 1143 1668 2353 3321 3512 33
Size: 220 890 525 685 968 191 1146
Fragments arranged by size:
1146 968 890 685 525 220 191
SfoI GGC'GCC
Cuts at: 577 792 969 1128 2057 2305 577
Size: 215 177 159 929 248 2897
Fragments arranged by size:
2897 929 248 215 177 159
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 616 1780 2695 3409 3671 616
Size: 1164 915 714 262 1570
Fragments arranged by size:
1570 1164 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 1678 2178 1678
Size: 500 4125
StuI AGG'CCT
Cuts at: 676 676
Size: 4625
StyI C'CwwG_G
Cuts at: 299 1122 1136 1759 2113 299
Size: 823 14 623 354 2811
Fragments arranged by size:
2811 823 623 354 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2208 2341 2800 3009 2208
Size: 133 459 209 3824
Fragments arranged by size:
3824 459 209 133
TatI w'GTAC_w
Cuts at: 1112 4140 1112
Size: 3028 1597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4142 4174 4142
Size: 32 4593
Tth111I GACn'n_nGTC
Cuts at: 897 2422 897
Size: 1525 3100
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 810 996 2120 810
Size: 186 1124 3315
Fragments arranged by size:
3315 1124 186
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmtI BsgI BsiWI BsmI BsmBI BspEI
BssHII BstEII BstZ17I Bsu36I CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI