Restriction map for
pF1KB9476
(Circular) (Six-base) MAPSORT of: pF1KB9476.seq Check: 6787 from: 1 to: 4160
pF1KB9476 4160 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 546 546
Size: 4160
AccI GT'mk_AC
Cuts at: 1198 1198
Size: 4160
Acc65I G'GTAC_C
Cuts at: 506 1176 506
Size: 670 3490
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 418 1985 2417 2770 3432 166
Size: 252 1567 432 353 662 894
Fragments arranged by size:
1567 894 662 432 353 252
AflIII A'CryG_T
Cuts at: 1512 3312 3593 1512
Size: 1800 281 2079
Fragments arranged by size:
2079 1800 281
AleI CACnn'nnGTG
Cuts at: 769 769
Size: 4160
AlwNI CAG_nnn'CTG
Cuts at: 630 1465 2903 630
Size: 835 1438 1887
Fragments arranged by size:
1887 1438 835
ApaI G_GGCC'C
Cuts at: 1093 1093
Size: 4160
ApaLI G'TGCA_C
Cuts at: 751 1042 2998 751
Size: 291 1956 1913
Fragments arranged by size:
1956 1913 291
ApoI r'AATT_y
Cuts at: 151 1164 3513 3769 151
Size: 1013 2349 256 542
Fragments arranged by size:
2349 1013 542 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 788 797 1131 1180 788
Size: 9 334 49 3768
Fragments arranged by size:
3768 334 49 9
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 226 506 534 792 1176 1590 1838 1873 226
Size: 280 28 258 384 414 248 35 2513
Fragments arranged by size:
2513 414 384 280 258 248 35 28
BanII G_rGCy'C
Cuts at: 470 569 764 1093 1174 2204 470
Size: 99 195 329 81 1030 2426
Fragments arranged by size:
2426 1030 329 195 99 81
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 601 601
Size: 4160
BciVI GTATCCnnnnn_n'
Cuts at: 2058 3114 4160 2058
Size: 1056 1046 2058
Fragments arranged by size:
2058 1056 1046
BclI T'GATC_A
Cuts at: 1680 1680
Size: 4160
BglI GCCn_nnn'nGGC
Cuts at: 421 670 1097 421
Size: 249 427 3484
Fragments arranged by size:
3484 427 249
BglII A'GATC_T
Cuts at: 1675 3712 1675
Size: 2037 2123
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 675 755 797 1046 1093 1785 1878 3002 675
Size: 80 42 249 47 692 93 1124 1833
Fragments arranged by size:
1833 1124 692 249 93 80 47 42
BmrI ACTGGGnnnn_n'
Cuts at: 1372 1453 1788 3932 1372
Size: 81 335 2144 1600
Fragments arranged by size:
2144 1600 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 661 909 994 553
Size: 108 248 85 3719
Fragments arranged by size:
3719 248 108 85
Bpu10I CC'TnA_GC
Cuts at: 341 341
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 877 1336 2215 2929 3227 670
Size: 207 459 879 714 298 1603
Fragments arranged by size:
1603 879 714 459 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 3864 3864
Size: 4160
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 1674 3711 6
Size: 1668 2037 455
Fragments arranged by size:
2037 1668 455
BsaHI Gr'CG_yC
Cuts at: 293 1591 1839 2541 4037 293
Size: 1298 248 702 1496 416
Fragments arranged by size:
1496 1298 702 416 248
BsaWI w'CCGG_w
Cuts at: 1349 1870 2548 2959 3106 3380 1349
Size: 521 678 411 147 274 2129
Fragments arranged by size:
2129 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 934 934
Size: 4160
BseYI C'CCAG_C
Cuts at: 222 251 296 502 665 776 1585 2343 3008
Size: 29 45 206 163 111 809 758 665
Cuts at: 3008 3416 222
Size: 408 966
Fragments arranged by size:
966 809 758 665 408 206 163 111
45 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 918 918
Size: 4160
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2978 104
Size: 1598 46 1230 1286
Fragments arranged by size:
1598 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 470 569 755 1046 1174 1952 2142 3002 470
Size: 99 186 291 128 778 190 860 1628
Fragments arranged by size:
1628 860 778 291 190 186 128 99
BsmI GAATG_Cn'
Cuts at: 342 920 342
Size: 578 3582
Bsp1286I G_dGCh'C
Cuts at: 470 569 675 755 764 797 1046 1093 1174
Size: 99 106 80 9 33 249 47 81
Cuts at: 1174 1785 1878 1952 2142 2204 3002 470
Size: 611 93 74 190 62 798 1628
Fragments arranged by size:
1628 798 611 249 190 106 99 93
81 80 74 62 47 33 9
BspHI T'CATG_A
Cuts at: 2592 2592
Size: 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 320 546 1210 1424 1726 2107 320
Size: 226 664 214 302 381 2373
Fragments arranged by size:
2373 664 381 302 226 214
BsrBI CCG'CTC
Cuts at: 50 732 2452 2506 3990 4155 50
Size: 682 1720 54 1484 165 55
Fragments arranged by size:
1720 1484 682 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2072 2072
Size: 4160
BsrFI r'CCGG_y
Cuts at: 2158 2339 2158
Size: 181 3979
BssSI C'ACGA_G
Cuts at: 217 2431 3139 217
Size: 2214 708 1238
Fragments arranged by size:
2214 1238 708
BstAPI GCAn_nnn'nTGC
Cuts at: 219 1537 219
Size: 1318 2842
BstBI TT'CG_AA
Cuts at: 350 2521 350
Size: 2171 1989
BstXI CCAn_nnnn'nTGG
Cuts at: 747 771 747
Size: 24 4136
BstYI r'GATC_y
Cuts at: 1 575 1185 1652 1675 2010 2256 2562 2574
Size: 574 610 467 23 335 246 306 12
Cuts at: 2574 2660 3712 1
Size: 86 1052 449
Fragments arranged by size:
1052 610 574 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 659 659
Size: 4160
BtgI C'CryG_G
Cuts at: 107 134 764 953 1125 107
Size: 27 630 189 172 3142
Fragments arranged by size:
3142 630 189 172 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1016 2110 2291 2454 1016
Size: 1094 181 163 2722
Fragments arranged by size:
2722 1094 181 163
BtsI GCAGTG_nn'
Cuts at: 283 1403 283
Size: 1120 3040
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1866 3210 1866
Size: 1344 2816
EaeI y'GGCC_r
Cuts at: 413 450 1745 1919 2310 2337 3682 413
Size: 37 1295 174 391 27 1345 891
Fragments arranged by size:
1345 1295 891 391 174 37 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 134 364 475 2183 2393 134
Size: 230 111 1708 210 1901
Fragments arranged by size:
1901 1708 230 210 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2368 3110 3256 3970 2368
Size: 742 146 714 2558
Fragments arranged by size:
2558 742 714 146
EcoICRI GAG'CTC
Cuts at: 468 567 1172 468
Size: 99 605 3456
Fragments arranged by size:
3456 605 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 418 553 661 909 994 1985 2417 2770
Size: 252 135 108 248 85 991 432 353
Cuts at: 2770 3432 166
Size: 662 894
Fragments arranged by size:
991 894 662 432 353 252 248 135
108 85
EcoO109I rG'GnC_Cy
Cuts at: 636 1089 1299 636
Size: 453 210 3497
Fragments arranged by size:
3497 453 210
EcoRI G'AATT_C
Cuts at: 151 1164 151
Size: 1013 3147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 171 1089 2179 171
Size: 918 1090 2152
Fragments arranged by size:
2152 1090 918
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 1119 1594 1842 3072 3843 1119
Size: 475 248 1230 771 1436
Fragments arranged by size:
1436 1230 771 475 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 2002 2032 2034 2064 478
Size: 32 1492 30 2 30 2574
Fragments arranged by size:
2574 1492 32 30 30 2
HincII GTy'rAC
Cuts at: 702 1199 702
Size: 497 3663
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 510 1180 510
Size: 670 3490
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2919 3103 2919
Size: 184 3976
MscI TGG'CCA
Cuts at: 452 1921 452
Size: 1469 2691
MslI CAynn'nnrTG
Cuts at: 712 769 839 2149 2276 712
Size: 57 70 1310 127 2596
Fragments arranged by size:
2596 1310 127 70 57
MspA1I CmG'CkG
Cuts at: 251 546 669 780 929 1127 1271 1585 1945
Size: 295 123 111 149 198 144 314 360
Cuts at: 1945 2729 2974 251
Size: 784 245 1437
Fragments arranged by size:
1437 784 360 314 295 245 198 149
144 123 111
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NcoI C'CATG_G
Cuts at: 107 107
Size: 4160
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NspI r_CATG'y
Cuts at: 1213 1713 3316 1213
Size: 500 1603 2057
Fragments arranged by size:
2057 1603 500
PasI CC'CwG_GG
Cuts at: 1093 1093
Size: 4160
PciI A'CATG_T
Cuts at: 3312 3312
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 4128
PspOMI G'GGCC_C
Cuts at: 1089 1089
Size: 4160
PstI C_TGCA'G
Cuts at: 635 656 1207 1892 635
Size: 21 551 685 2903
Fragments arranged by size:
2903 685 551 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 780 929 1585 1945 780
Size: 149 656 360 2995
Fragments arranged by size:
2995 656 360 149
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 470 569 1174 470
Size: 99 605 3456
Fragments arranged by size:
3456 605 99
SacII CC_GC'GG
Cuts at: 1128 1128
Size: 4160
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 134 364 475 2183 2393 134
Size: 230 111 1708 210 1901
Fragments arranged by size:
1901 1708 230 210 111
SbfI CC_TGCA'GG
Cuts at: 635 1207 635
Size: 572 3588
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SfcI C'TryA_G
Cuts at: 33 631 652 863 1203 1888 2856 3047 33
Size: 598 21 211 340 685 968 191 1146
Fragments arranged by size:
1146 968 685 598 340 211 191 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 421 421
Size: 4160
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 790 1133 1182 790
Size: 343 49 3768
Fragments arranged by size:
3768 343 49
SmlI C'TyrA_G
Cuts at: 685 856 1315 2230 2944 3206 685
Size: 171 459 915 714 262 1639
Fragments arranged by size:
1639 915 714 459 262 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 1213 1713 1213
Size: 500 3660
StuI AGG'CCT
Cuts at: 1072 1072
Size: 4160
StyI C'CwwG_G
Cuts at: 107 161 1085 1294 1648 107
Size: 54 924 209 354 2619
Fragments arranged by size:
2619 924 354 209 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 214 853 1743 1876 2335 2544 214
Size: 639 890 133 459 209 1830
Fragments arranged by size:
1830 890 639 459 209 133
TatI w'GTAC_w
Cuts at: 3675 3675
Size: 4160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 508 540 3677 3709 508
Size: 32 3137 32 959
Fragments arranged by size:
3137 959 32 32
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 248 1655 248
Size: 1407 2753
XmaI C'CCGG_G
Cuts at: 788 1131 1180 788
Size: 343 49 3768
Fragments arranged by size:
3768 343 49
XmnI GAAnn'nnTTC
Cuts at: 151 151
Size: 4160
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SanDI
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI