Restriction map for
pF1KB9479
(Circular) (Six-base) MAPSORT of: pF1KB9479.seq Check: 5275 from: 1 to: 5306
pF1KB9479 5306 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2034 2034
Size: 5306
AccI GT'mk_AC
Cuts at: 2344 2344
Size: 5306
Acc65I G'GTAC_C
Cuts at: 2322 2322
Size: 5306
AclI AA'CG_TT
Cuts at: 5146 5146
Size: 5306
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 1270 2157 2221 3131 3563 3916 4578 979
Size: 291 887 64 910 432 353 662 1707
Fragments arranged by size:
1707 910 887 662 432 353 291 64
AflIII A'CryG_T
Cuts at: 695 2658 4458 4739 695
Size: 1963 1800 281 1262
Fragments arranged by size:
1963 1800 1262 281
AleI CACnn'nnGTG
Cuts at: 436 565 436
Size: 129 5177
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1229 1261 1229
Size: 32 5274
AlwNI CAG_nnn'CTG
Cuts at: 127 208 468 495 766 1002 1107 1128 1341
Size: 81 260 27 271 236 105 21 213
Cuts at: 1341 1425 1434 1950 2611 4049 127
Size: 84 9 516 661 1438 1384
Fragments arranged by size:
1438 1384 661 516 271 260 236 213
105 84 81 27 21 9
ApaI G_GGCC'C
Cuts at: 184 714 779 1116 1153 1720 2258 184
Size: 530 65 337 37 567 538 3232
Fragments arranged by size:
3232 567 538 530 337 65 37
ApaLI G'TGCA_C
Cuts at: 4144 4144
Size: 5306
ApoI r'AATT_y
Cuts at: 1509 2310 4659 4915 1509
Size: 801 2349 256 1900
Fragments arranged by size:
2349 1900 801 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5306
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5306
AvaI C'yCGr_G
Cuts at: 814 2326 814
Size: 1512 3794
BamHI G'GATC_C
Cuts at: 2331 2331
Size: 5306
BanI G'GyrC_C
Cuts at: 352 627 1487 1857 2322 2736 2984 3019 352
Size: 275 860 370 465 414 248 35 2639
Fragments arranged by size:
2639 860 465 414 370 275 248 35
BanII G_rGCy'C
Cuts at: 184 219 484 714 779 1056 1086 1116 1153
Size: 35 265 230 65 277 30 30 37
Cuts at: 1153 1720 2242 2258 2320 3350 184
Size: 567 522 16 62 1030 2140
Fragments arranged by size:
2140 1030 567 522 277 265 230 65
62 37 35 30 30 16
BbeI G_GCGC'C
Cuts at: 2740 2988 2740
Size: 248 5058
BbsI GAAGACnn'nnnn_
Cuts at: 907 907
Size: 5306
BbvCI CC'TCA_GC
Cuts at: 617 1274 617
Size: 657 4649
BciVI GTATCCnnnnn_n'
Cuts at: 3204 4260 5306 3204
Size: 1056 1046 3204
Fragments arranged by size:
3204 1056 1046
BclI T'GATC_A
Cuts at: 2826 2826
Size: 5306
BglI GCCn_nnn'nGGC
Cuts at: 223 1020 1480 223
Size: 797 460 4049
Fragments arranged by size:
4049 797 460
BglII A'GATC_T
Cuts at: 2821 4858 2821
Size: 2037 3269
BlpI GC'TnA_GC
Cuts at: 1397 1568 2418 1397
Size: 171 850 4285
Fragments arranged by size:
4285 850 171
Bme1580I G_kGCm'C
Cuts at: 184 355 632 714 779 1116 1153 1720 1860
Size: 171 277 82 65 337 37 567 140
Cuts at: 1860 1893 2258 2270 2931 3024 4148 184
Size: 33 365 12 661 93 1124 1342
Fragments arranged by size:
1342 1124 661 567 365 337 277 171
140 93 82 65 37 33 12
BmgBI CAC'GTC
Cuts at: 567 567
Size: 5306
BmrI ACTGGGnnnn_n'
Cuts at: 187 782 868 1452 1629 1927 2518 2599 2934
Size: 595 86 584 177 298 591 81 335
Cuts at: 2934 5078 187
Size: 2144 415
Fragments arranged by size:
2144 595 591 584 415 335 298 177
86 81
BmtI G_CTAG'C
Cuts at: 1930 1930
Size: 5306
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1971 3673 1971
Size: 1702 3604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 1105 1108 1150 1288 1363 1456 1781 2116
Size: 577 3 42 138 75 93 325 335
Cuts at: 2116 528
Size: 3718
Fragments arranged by size:
3718 577 335 325 138 93 75 42
3
Bpu10I CC'TnA_GC
Cuts at: 617 853 1274 1872 617
Size: 236 421 598 4051
Fragments arranged by size:
4051 598 421 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2482 3361 4075 4373 2482
Size: 879 714 298 3415
Fragments arranged by size:
3415 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 410 421 1467 1550 5010 410
Size: 11 1046 83 3460 706
Fragments arranged by size:
3460 1046 706 83 11
BsaAI yAC'GTr
Cuts at: 2659 2659
Size: 5306
BsaBI GATnn'nnATC
Cuts at: 6 2820 4857 6
Size: 2814 2037 455
Fragments arranged by size:
2814 2037 455
BsaHI Gr'CG_yC
Cuts at: 2737 2985 3687 5183 2737
Size: 248 702 1496 2860
Fragments arranged by size:
2860 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1559 2495 3016 3694 4105 4252 4526 1559
Size: 936 521 678 411 147 274 2339
Fragments arranged by size:
2339 936 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 389 419 223
Size: 30 136 30 5110
Fragments arranged by size:
5110 136 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 349 544 730 1042 1045 1362 1696 1746 2125
Size: 195 186 312 3 317 334 50 379
Cuts at: 2125 349
Size: 3530
Fragments arranged by size:
3530 379 334 317 312 195 186 50
3
BseYI C'CCAG_C
Cuts at: 137 265 652 678 895 968 1040 1320 1377
Size: 128 387 26 217 73 72 280 57
Cuts at: 1377 1401 1993 2731 3489 4154 4562 137
Size: 24 592 738 758 665 408 881
Fragments arranged by size:
881 758 738 665 592 408 387 280
217 128 73 72 57 26 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 686
Size: 5306
BsiEI CG_ry'CG
Cuts at: 104 2848 2894 4124 104
Size: 2744 46 1230 1286
Fragments arranged by size:
2744 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1574 2320 3098 3288 4148 1574
Size: 746 778 190 860 2732
Fragments arranged by size:
2732 860 778 746 190
BsmI GAATG_Cn'
Cuts at: 323 1041 323
Size: 718 4588
BsmBI CGTCTCn'nnnn_
Cuts at: 582 582
Size: 5306
Bsp1286I G_dGCh'C
Cuts at: 184 219 355 484 632 714 779 1056 1086
Size: 35 136 129 148 82 65 277 30
Cuts at: 1086 1116 1153 1574 1720 1860 1893 2242 2258
Size: 30 37 421 146 140 33 349 16
Cuts at: 2258 2270 2320 2931 3024 3098 3288 3350 4148
Size: 12 50 611 93 74 190 62 798
Cuts at: 4148 184
Size: 1342
Fragments arranged by size:
1342 798 611 421 349 277 190 148
146 140 136 129 93 82 74 65
62 50 37 35 33 30 30 16
12
BspHI T'CATG_A
Cuts at: 2058 3738 2058
Size: 1680 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 428 789 2034 2356 2570 2872 3253 428
Size: 361 1245 322 214 302 381 2481
Fragments arranged by size:
2481 1245 381 361 322 302 214
BsrBI CCG'CTC
Cuts at: 50 795 3598 3652 5136 5301 50
Size: 745 2803 54 1484 165 55
Fragments arranged by size:
2803 1484 745 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3218 3218
Size: 5306
BsrFI r'CCGG_y
Cuts at: 607 3304 3485 607
Size: 2697 181 2428
Fragments arranged by size:
2697 2428 181
BsrGI T'GTAC_A
Cuts at: 1329 1329
Size: 5306
BssSI C'ACGA_G
Cuts at: 3577 4285 3577
Size: 708 4598
BstAPI GCAn_nnn'nTGC
Cuts at: 1107 2683 1107
Size: 1576 3730
BstBI TT'CG_AA
Cuts at: 3667 3667
Size: 5306
BstXI CCAn_nnnn'nTGG
Cuts at: 1456 1759 2185 1456
Size: 303 426 4577
Fragments arranged by size:
4577 426 303
BstYI r'GATC_y
Cuts at: 1 1338 2331 2798 2821 3156 3402 3708 3720
Size: 1337 993 467 23 335 246 306 12
Cuts at: 3720 3806 4858 1
Size: 86 1052 449
Fragments arranged by size:
1337 1052 993 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 272 434 623 779 1368 107
Size: 165 162 189 156 589 4045
Fragments arranged by size:
4045 589 189 165 162 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3256 3437 3600 3256
Size: 181 163 4962
Fragments arranged by size:
4962 181 163
BtsI GCAGTG_nn'
Cuts at: 555 2549 555
Size: 1994 3312
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1765 1800 1765
Size: 35 5271
DraI TTT'AAA
Cuts at: 2305 2305
Size: 5306
DraIII CAC_nnn'GTG
Cuts at: 1192 1192
Size: 5306
DrdI GACnn_nn'nnGTC
Cuts at: 406 1459 3012 4356 406
Size: 1053 1553 1344 1356
Fragments arranged by size:
1553 1356 1344 1053
EaeI y'GGCC_r
Cuts at: 932 1599 2891 3065 3456 3483 4828 932
Size: 667 1292 174 391 27 1345 1410
Fragments arranged by size:
1410 1345 1292 667 391 174 27
EagI C'GGCC_G
Cuts at: 2891 2891
Size: 5306
EarI CTCTTCn'nnn_
Cuts at: 298 748 983 2200 3329 3539 298
Size: 450 235 1217 1129 210 2065
Fragments arranged by size:
2065 1217 1129 450 235 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3514 4256 4402 5116 3514
Size: 742 146 714 3704
Fragments arranged by size:
3704 742 714 146
EcoICRI GAG'CTC
Cuts at: 2318 2318
Size: 5306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 979 1105 1108 1150 1270 1288 1363 1456
Size: 451 126 3 42 120 18 75 93
Cuts at: 1456 1781 2116 2157 2221 3131 3563 3916 4578
Size: 325 335 41 64 910 432 353 662
Cuts at: 4578 528
Size: 1256
Fragments arranged by size:
1256 910 662 451 432 353 335 325
126 120 93 75 64 42 41 18
3
EcoNI CCTnn'n_nnAGG
Cuts at: 392 392
Size: 5306
EcoO109I rG'GnC_Cy
Cuts at: 225 465 710 711 735 801 870 1149 1216
Size: 240 245 1 24 66 69 279 67
Cuts at: 1216 1374 1431 1452 1716 1902 2254 2445 225
Size: 158 57 21 264 186 352 191 3086
Fragments arranged by size:
3086 352 279 264 245 240 191 186
158 69 67 66 57 24 21 1
EcoRI G'AATT_C
Cuts at: 1509 2310 1509
Size: 801 4505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1518 3325 1518
Size: 1807 3499
FseI GG_CCGG'CC
Cuts at: 611 611
Size: 5306
FspI TGC'GCA
Cuts at: 3087 3087
Size: 5306
HaeII r_GCGC'y
Cuts at: 2740 2988 4218 4989 2740
Size: 248 1230 771 3057
Fragments arranged by size:
3057 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 2215 2247 3148 3178 3180 3210 190
Size: 32 1993 32 901 30 2 30 2286
Fragments arranged by size:
2286 1993 901 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2345 2345
Size: 5306
KasI G'GCGC_C
Cuts at: 2736 2984 2736
Size: 248 5058
KpnI G_GTAC'C
Cuts at: 2326 2326
Size: 5306
MluI A'CGCG_T
Cuts at: 4739 4739
Size: 5306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4065 4249 4065
Size: 184 5122
MscI TGG'CCA
Cuts at: 934 3067 934
Size: 2133 3173
MslI CAynn'nnrTG
Cuts at: 436 565 3295 3422 436
Size: 129 2730 127 2320
Fragments arranged by size:
2730 2320 129 127
MspA1I CmG'CkG
Cuts at: 539 864 972 1080 1107 1266 1370 2001 2417
Size: 325 108 108 27 159 104 631 416
Cuts at: 2417 2731 3091 3875 4120 539
Size: 314 360 784 245 1725
Fragments arranged by size:
1725 784 631 416 360 325 314 245
159 108 108 104 27
NaeI GCC'GGC
Cuts at: 609 3487 609
Size: 2878 2428
NarI GG'CG_CC
Cuts at: 2737 2985 2737
Size: 248 5058
NcoI C'CATG_G
Cuts at: 107 272 623 779 107
Size: 165 351 156 4634
Fragments arranged by size:
4634 351 165 156
NgoMIV G'CCGG_C
Cuts at: 607 3485 607
Size: 2878 2428
NheI G'CTAG_C
Cuts at: 1926 1926
Size: 5306
NspI r_CATG'y
Cuts at: 699 2359 2859 4462 699
Size: 1660 500 1603 1543
Fragments arranged by size:
1660 1603 1543 500
PasI CC'CwG_GG
Cuts at: 1220 1456 1863 1988 2258 1220
Size: 236 407 125 270 4268
Fragments arranged by size:
4268 407 270 236 125
PciI A'CATG_T
Cuts at: 695 4458 695
Size: 3763 1543
PflMI CCAn_nnn'nTGG
Cuts at: 1128 1413 1128
Size: 285 5021
PfoI T'CCnGG_A
Cuts at: 190 2075 190
Size: 1885 3421
PmeI GTTT'AAAC
Cuts at: 2305 2305
Size: 5306
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 5274
PpuMI rG'GwC_Cy
Cuts at: 465 735 801 1374 1452 465
Size: 270 66 573 78 4319
Fragments arranged by size:
4319 573 270 78 66
PshAI GACnn'nnGTC
Cuts at: 343 343
Size: 5306
PspOMI G'GGCC_C
Cuts at: 180 710 775 1112 1149 1716 2254 180
Size: 530 65 337 37 567 538 3232
Fragments arranged by size:
3232 567 538 530 337 65 37
PstI C_TGCA'G
Cuts at: 224 425 869 965 977 1061 1103 1430 2353
Size: 201 444 96 12 84 42 327 923
Cuts at: 2353 3038 224
Size: 685 2492
Fragments arranged by size:
2492 923 685 444 327 201 96 84
42 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5306
PvuII CAG'CTG
Cuts at: 864 972 1107 1266 2001 2731 3091 864
Size: 108 135 159 735 730 360 3079
Fragments arranged by size:
3079 735 730 360 159 135 108
RsrII CG'GwC_CG
Cuts at: 3501 3501
Size: 5306
SacI G_AGCT'C
Cuts at: 2320 2320
Size: 5306
SacII CC_GC'GG
Cuts at: 1371 1371
Size: 5306
SalI G'TCGA_C
Cuts at: 2343 2343
Size: 5306
SanDI GG'GwC_CC
Cuts at: 1374 1374
Size: 5306
SapI GCTCTTCn'nnn_
Cuts at: 298 983 3329 3539 298
Size: 685 2346 210 2065
Fragments arranged by size:
2346 2065 685 210
SbfI CC_TGCA'GG
Cuts at: 224 425 2353 224
Size: 201 1928 3177
Fragments arranged by size:
3177 1928 201
ScaI AGT'ACT
Cuts at: 4823 4823
Size: 5306
SfcI C'TryA_G
Cuts at: 33 220 421 865 961 973 1057 1099 1426
Size: 187 201 444 96 12 84 42 327
Cuts at: 1426 2349 3034 4002 4193 33
Size: 923 685 968 191 1146
Fragments arranged by size:
1146 968 923 685 444 327 201 191
187 96 84 42 12
SfoI GGC'GCC
Cuts at: 2738 2986 2738
Size: 248 5058
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5306
SmaI CCC'GGG
Cuts at: 2328 2328
Size: 5306
SmlI C'TyrA_G
Cuts at: 2461 3376 4090 4352 2461
Size: 915 714 262 3415
Fragments arranged by size:
3415 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5306
SphI G_CATG'C
Cuts at: 2359 2859 2359
Size: 500 4806
StuI AGG'CCT
Cuts at: 446 1483 446
Size: 1037 4269
StyI C'CwwG_G
Cuts at: 107 272 623 705 779 935 1672 1732 2006
Size: 165 351 82 74 156 737 60 274
Cuts at: 2006 2440 2794 107
Size: 434 354 2619
Fragments arranged by size:
2619 737 434 354 351 274 165 156
82 74 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 2889 3022 3481 3690 129
Size: 2760 133 459 209 1745
Fragments arranged by size:
2760 1745 459 209 133
TatI w'GTAC_w
Cuts at: 1329 4821 1329
Size: 3492 1814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 223 255 1480 1512 1525 1557 4823 4855 223
Size: 32 1225 32 13 32 3266 32 674
Fragments arranged by size:
3266 1225 674 32 32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 889 1141 3103 889
Size: 252 1962 3092
Fragments arranged by size:
3092 1962 252
XbaI T'CTAG_A
Cuts at: 2337 2337
Size: 5306
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 630 786 1652 2801 630
Size: 156 866 1149 3135
Fragments arranged by size:
3135 1149 866 156
XmaI C'CCGG_G
Cuts at: 2326 2326
Size: 5306
XmnI GAAnn'nnTTC
Cuts at: 992 992
Size: 5306
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AscI AvrII
BaeI BcgI BfrBI BsiWI BspEI BssHII BstEII BstZ17I
Bsu36I ClaI EcoRV FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PmlI PsiI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI