Restriction map for
pF1KB9480
(Circular) (Six-base) MAPSORT of: pF1KB9480.seq Check: 3149 from: 1 to: 5609
pF1KB9480 5609 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1083 1279 2035 1083
Size: 196 756 4657
Fragments arranged by size:
4657 756 196
AccI GT'mk_AC
Cuts at: 767 780 1592 2081 2647 767
Size: 13 812 489 566 3729
Fragments arranged by size:
3729 812 566 489 13
Acc65I G'GTAC_C
Cuts at: 2625 2625
Size: 5609
AclI AA'CG_TT
Cuts at: 5449 5449
Size: 5609
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 489 1156 1255 2089 3434 3866 4613 4881
Size: 112 667 99 834 1345 432 747 268
Cuts at: 4881 377
Size: 1105
Fragments arranged by size:
1345 1105 834 747 667 432 268 112
99
AflIII A'CryG_T
Cuts at: 1247 2961 4065 5042 1247
Size: 1714 1104 977 1814
Fragments arranged by size:
1814 1714 1104 977
AgeI A'CCGG_T
Cuts at: 419 3997 419
Size: 3578 2031
AhdI GACnn_n'nnGTC
Cuts at: 2562 2562
Size: 5609
AleI CACnn'nnGTG
Cuts at: 2248 2248
Size: 5609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 220 643 220
Size: 423 5186
AlwNI CAG_nnn'CTG
Cuts at: 213 1134 2356 2433 2914 4481 213
Size: 921 1222 77 481 1567 1341
Fragments arranged by size:
1567 1341 1222 921 481 77
ApaLI G'TGCA_C
Cuts at: 553 4379 553
Size: 3826 1783
ApoI r'AATT_y
Cuts at: 1750 2613 4962 5218 1750
Size: 863 2349 256 2141
Fragments arranged by size:
2349 2141 863 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5609
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5609
AvaI C'yCGr_G
Cuts at: 2139 2629 2139
Size: 490 5119
BamHI G'GATC_C
Cuts at: 2634 2634
Size: 5609
BanI G'GyrC_C
Cuts at: 136 1804 2625 3039 3287 3322 136
Size: 1668 821 414 248 35 2423
Fragments arranged by size:
2423 1668 821 414 248 35
BanII G_rGCy'C
Cuts at: 2054 2472 2482 2495 2623 3653 2054
Size: 418 10 13 128 1030 4010
Fragments arranged by size:
4010 1030 418 128 13 10
BbeI G_GCGC'C
Cuts at: 1808 3043 3291 1808
Size: 1235 248 4126
Fragments arranged by size:
4126 1235 248
BbsI GAAGACnn'nnnn_
Cuts at: 1148 1989 1148
Size: 841 4768
BbvCI CC'TCA_GC
Cuts at: 1760 1833 1760
Size: 73 5536
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 897 931 90
Size: 34 773 34 4768
Fragments arranged by size:
4768 773 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3507 4268 5609 3507
Size: 761 1341 3507
Fragments arranged by size:
3507 1341 761
BclI T'GATC_A
Cuts at: 1037 3129 1037
Size: 2092 3517
BglI GCCn_nnn'nGGC
Cuts at: 2049 2049
Size: 5609
BglII A'GATC_T
Cuts at: 3124 5161 3124
Size: 2037 3572
BlpI GC'TnA_GC
Cuts at: 2721 2721
Size: 5609
Bme1580I G_kGCm'C
Cuts at: 139 557 2045 2322 3234 3327 4383 139
Size: 418 1488 277 912 93 1056 1365
Fragments arranged by size:
1488 1365 1056 912 418 277 93
BmgBI CAC'GTC
Cuts at: 2234 2289 2234
Size: 55 5554
BmrI ACTGGGnnnn_n'
Cuts at: 142 1374 1530 2821 2902 3237 5381 142
Size: 1232 156 1291 81 335 2144 370
Fragments arranged by size:
2144 1291 1232 370 335 156 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1392 3976 1392
Size: 2584 3025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 676 994 1144 1442 429
Size: 247 318 150 298 4596
Fragments arranged by size:
4596 318 298 247 150
Bpu10I CC'TnA_GC
Cuts at: 1760 1833 2426 2481 1760
Size: 73 593 55 4888
Fragments arranged by size:
4888 593 73 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2785 3664 4156 4454 2785
Size: 879 492 298 3940
Fragments arranged by size:
3940 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5313 5313
Size: 5609
BsaAI yAC'GTr
Cuts at: 2962 2962
Size: 5609
BsaBI GATnn'nnATC
Cuts at: 6 3123 5160 6
Size: 3117 2037 455
Fragments arranged by size:
3117 2037 455
BsaHI Gr'CG_yC
Cuts at: 227 605 1805 2540 3040 3288 3990 5486 227
Size: 378 1200 735 500 248 702 1496 350
Fragments arranged by size:
1496 1200 735 702 500 378 350 248
BsaWI w'CCGG_w
Cuts at: 293 419 2798 3319 3997 4271 4418 4829 293
Size: 126 2379 521 678 274 147 411 1073
Fragments arranged by size:
2379 1073 678 521 411 274 147 126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1429 2533 1429
Size: 1104 4505
BseYI C'CCAG_C
Cuts at: 1701 3034 3792 4369 4865 1701
Size: 1333 758 577 496 2445
Fragments arranged by size:
2445 1333 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 891 1251 1297 712
Size: 179 360 46 5024
Fragments arranged by size:
5024 360 179 46
BsiEI CG_ry'CG
Cuts at: 104 1919 3151 3197 4405 104
Size: 1815 1232 46 1208 1308
Fragments arranged by size:
1815 1308 1232 1208 46
BsiHKAI G_wGCw'C
Cuts at: 557 1373 2342 2482 2623 3401 3591 4383 557
Size: 816 969 140 141 778 190 792 1783
Fragments arranged by size:
1783 969 816 792 778 190 141 140
BsmBI CGTCTCn'nnnn_
Cuts at: 436 436
Size: 5609
Bsp1286I G_dGCh'C
Cuts at: 139 557 1373 2045 2054 2322 2342 2472 2482
Size: 418 816 672 9 268 20 130 10
Cuts at: 2482 2495 2623 3234 3327 3401 3591 3653 4383
Size: 13 128 611 93 74 190 62 730
Cuts at: 4383 139
Size: 1365
Fragments arranged by size:
1365 816 730 672 611 418 268 190
130 128 93 74 62 20 13 10
9
BspEI T'CCGG_A
Cuts at: 293 4829 293
Size: 4536 1073
BspHI T'CATG_A
Cuts at: 2009 4785 2009
Size: 2776 2833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 659 725 1083 1279 1868 2035 2117 2659 2873
Size: 66 358 196 589 167 82 542 214
Cuts at: 2873 3175 3556 659
Size: 302 381 2712
Fragments arranged by size:
2712 589 542 381 358 302 214 196
167 82 66
BsrBI CCG'CTC
Cuts at: 50 984 3901 3955 5439 5604 50
Size: 934 2917 54 1484 165 55
Fragments arranged by size:
2917 1484 934 165 55 54
BsrDI GCAATG_nn'
Cuts at: 689 1826 2379 3521 689
Size: 1137 553 1142 2777
Fragments arranged by size:
2777 1142 1137 553
BsrFI r'CCGG_y
Cuts at: 419 2112 3607 3788 3997 419
Size: 1693 1495 181 209 2031
Fragments arranged by size:
2031 1693 1495 209 181
BsrGI T'GTAC_A
Cuts at: 1886 2057 1886
Size: 171 5438
BssHII G'CGCG_C
Cuts at: 1909 1909
Size: 5609
BssSI C'ACGA_G
Cuts at: 3880 4238 3880
Size: 358 5251
BstAPI GCAn_nnn'nTGC
Cuts at: 213 2986 213
Size: 2773 2836
BstBI TT'CG_AA
Cuts at: 3970 3970
Size: 5609
BstXI CCAn_nnnn'nTGG
Cuts at: 240 1506 240
Size: 1266 4343
BstYI r'GATC_y
Cuts at: 1 524 2132 2634 3101 3124 3459 3705 4717
Size: 523 1608 502 467 23 335 246 1012
Cuts at: 4717 4803 4815 5161 1
Size: 86 12 346 449
Fragments arranged by size:
1608 1012 523 502 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 1275 1478 1853 1275
Size: 203 375 5031
Fragments arranged by size:
5031 375 203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 560 2406 3559 3740 3903 560
Size: 1846 1153 181 163 2266
Fragments arranged by size:
2266 1846 1153 181 163
BtsI GCAGTG_nn'
Cuts at: 268 2387 2852 268
Size: 2119 465 3025
Fragments arranged by size:
3025 2119 465
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1035 1070 1035
Size: 35 5574
DraI TTT'AAA
Cuts at: 2608 2608
Size: 5609
DrdI GACnn_nn'nnGTC
Cuts at: 3315 4173 3315
Size: 858 4751
EaeI y'GGCC_r
Cuts at: 125 1056 1916 2114 2162 3194 3368 3759 3786
Size: 931 860 198 48 1032 174 391 27
Cuts at: 3786 5131 125
Size: 1345 603
Fragments arranged by size:
1345 1032 931 860 603 391 198 174
48 27
EagI C'GGCC_G
Cuts at: 1916 3194 1916
Size: 1278 4331
EarI CTCTTCn'nnn_
Cuts at: 838 1435 1447 1729 2188 2200 3632 3842 838
Size: 597 12 282 459 12 1432 210 2605
Fragments arranged by size:
2605 1432 597 459 282 210 12 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3817 4127 4273 5419 3817
Size: 310 146 1146 4007
Fragments arranged by size:
4007 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2480 2621 2480
Size: 141 5468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 429 489 676 994 1144 1156 1255 1442
Size: 52 60 187 318 150 12 99 187
Cuts at: 1442 2089 3434 3866 4613 4881 377
Size: 647 1345 432 747 268 1105
Fragments arranged by size:
1345 1105 747 647 432 318 268 187
187 150 99 60 52 12
EcoNI CCTnn'n_nnAGG
Cuts at: 295 295
Size: 5609
EcoO109I rG'GnC_Cy
Cuts at: 210 648 2748 210
Size: 438 2100 3071
Fragments arranged by size:
3071 2100 438
EcoRI G'AATT_C
Cuts at: 2613 2613
Size: 5609
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1014 1770 3628 1014
Size: 756 1858 2995
Fragments arranged by size:
2995 1858 756
FspI TGC'GCA
Cuts at: 1867 2212 3390 1867
Size: 345 1178 4086
Fragments arranged by size:
4086 1178 345
FspAI rTGC'GCAy
Cuts at: 2212 2212
Size: 5609
HaeII r_GCGC'y
Cuts at: 185 1808 3043 3291 4313 5292 185
Size: 1623 1235 248 1022 979 502
Fragments arranged by size:
1623 1235 1022 979 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1376 1408 2482 2514 2548 2580 3451 3481 3483
Size: 32 1074 32 34 32 871 30 2
Cuts at: 3483 3513 1376
Size: 30 3472
Fragments arranged by size:
3472 1074 871 34 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 456 636 2648 456
Size: 180 2012 3417
Fragments arranged by size:
3417 2012 180
KasI G'GCGC_C
Cuts at: 1804 3039 3287 1804
Size: 1235 248 4126
Fragments arranged by size:
4126 1235 248
KpnI G_GTAC'C
Cuts at: 2629 2629
Size: 5609
MluI A'CGCG_T
Cuts at: 5042 5042
Size: 5609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1346 4280 4464 1346
Size: 2934 184 2491
Fragments arranged by size:
2934 2491 184
MscI TGG'CCA
Cuts at: 127 1058 3370 127
Size: 931 2312 2366
Fragments arranged by size:
2366 2312 931
MslI CAynn'nnrTG
Cuts at: 1477 1678 1888 2038 2059 2236 2248 3598 3725
Size: 201 210 150 21 177 12 1350 127
Cuts at: 3725 1477
Size: 3361
Fragments arranged by size:
3361 1350 210 201 177 150 127 21
12
MspA1I CmG'CkG
Cuts at: 573 1733 2720 3034 3394 4407 4652 573
Size: 1160 987 314 360 1013 245 1530
Fragments arranged by size:
1530 1160 1013 987 360 314 245
NaeI GCC'GGC
Cuts at: 2114 3790 2114
Size: 1676 3933
NarI GG'CG_CC
Cuts at: 1805 3040 3288 1805
Size: 1235 248 4126
Fragments arranged by size:
4126 1235 248
NcoI C'CATG_G
Cuts at: 1478 1853 1478
Size: 375 5234
NgoMIV G'CCGG_C
Cuts at: 2112 3788 2112
Size: 1676 3933
NspI r_CATG'y
Cuts at: 777 2064 2662 3162 4069 777
Size: 1287 598 500 907 2317
Fragments arranged by size:
2317 1287 907 598 500
PciI A'CATG_T
Cuts at: 4065 4065
Size: 5609
PflMI CCAn_nnn'nTGG
Cuts at: 2160 2160
Size: 5609
PfoI T'CCnGG_A
Cuts at: 527 527
Size: 5609
PmeI GTTT'AAAC
Cuts at: 2608 2608
Size: 5609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1771 1803 4748 4780 1771
Size: 32 2945 32 2600
Fragments arranged by size:
2945 2600 32 32
PpuMI rG'GwC_Cy
Cuts at: 648 648
Size: 5609
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 652 684 652
Size: 32 5577
PstI C_TGCA'G
Cuts at: 209 1303 2438 2656 3341 209
Size: 1094 1135 218 685 2477
Fragments arranged by size:
2477 1135 1094 685 218
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5609
PvuII CAG'CTG
Cuts at: 3034 3394 3034
Size: 360 5249
RsrII CG'GwC_CG
Cuts at: 3804 3804
Size: 5609
SacI G_AGCT'C
Cuts at: 2482 2623 2482
Size: 141 5468
SalI G'TCGA_C
Cuts at: 2646 2646
Size: 5609
SapI GCTCTTCn'nnn_
Cuts at: 2200 3632 3842 2200
Size: 1432 210 3967
Fragments arranged by size:
3967 1432 210
SbfI CC_TGCA'GG
Cuts at: 209 2656 209
Size: 2447 3162
ScaI AGT'ACT
Cuts at: 1111 5126 1111
Size: 4015 1594
SexAI A'CCwGG_T
Cuts at: 2075 2153 2600 2075
Size: 78 447 5084
Fragments arranged by size:
5084 447 78
SfcI C'TryA_G
Cuts at: 33 177 205 669 768 1299 2016 2434 2652
Size: 144 28 464 99 531 717 418 218
Cuts at: 2652 3337 4330 4521 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 717 685 531 464 418 218
191 144 99 28
SfoI GGC'GCC
Cuts at: 1806 3041 3289 1806
Size: 1235 248 4126
Fragments arranged by size:
4126 1235 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5609
SmaI CCC'GGG
Cuts at: 2631 2631
Size: 5609
SmlI C'TyrA_G
Cuts at: 2764 3679 4171 4433 2764
Size: 915 492 262 3940
Fragments arranged by size:
3940 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5609
SphI G_CATG'C
Cuts at: 777 2662 3162 777
Size: 1885 500 3224
Fragments arranged by size:
3224 1885 500
StuI AGG'CCT
Cuts at: 1877 1951 1877
Size: 74 5535
StyI C'CwwG_G
Cuts at: 164 326 1478 1853 2743 3097 164
Size: 162 1152 375 890 354 2676
Fragments arranged by size:
2676 1152 890 375 354 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 383 1549 3192 3325 3784 3993 383
Size: 1166 1643 133 459 209 1999
Fragments arranged by size:
1999 1643 1166 459 209 133
TatI w'GTAC_w
Cuts at: 995 1109 1886 2057 5124 995
Size: 114 777 171 3067 1480
Fragments arranged by size:
3067 1480 777 171 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 547 579 5126 5158 547
Size: 32 4547 32 998
Fragments arranged by size:
4547 998 32 32
Tth111I GACn'n_nGTC
Cuts at: 1332 3406 1332
Size: 2074 3535
XbaI T'CTAG_A
Cuts at: 2640 2640
Size: 5609
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 472 2293 3104 472
Size: 1821 811 2977
Fragments arranged by size:
2977 1821 811
XmaI C'CCGG_G
Cuts at: 2629 2629
Size: 5609
XmnI GAAnn'nnTTC
Cuts at: 1240 1240
Size: 5609
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI ApaI AscI AvrII BaeI
BfrBI BmtI BsaXI BsiWI BsmI BstEII BstZ17I Bsu36I
ClaI DraIII EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI