Restriction map for
pF1KB9482
(Circular) (Six-base) MAPSORT of: pF1KB9482.seq Check: 7069 from: 1 to: 4664
pF1KB9482 4664 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1455 1455
Size: 4664
AccI GT'mk_AC
Cuts at: 1414 1702 1414
Size: 288 4376
Acc65I G'GTAC_C
Cuts at: 1680 1680
Size: 4664
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 2489 2921 3274 3936 612
Size: 1877 432 353 662 1340
Fragments arranged by size:
1877 1340 662 432 353
AflIII A'CryG_T
Cuts at: 470 926 1580 2016 3816 4097 470
Size: 456 654 436 1800 281 1037
Fragments arranged by size:
1800 1037 654 456 436 281
AhdI GACnn_n'nnGTC
Cuts at: 927 927
Size: 4664
AlwNI CAG_nnn'CTG
Cuts at: 1401 1969 3407 1401
Size: 568 1438 2658
Fragments arranged by size:
2658 1438 568
ApaI G_GGCC'C
Cuts at: 1001 1001
Size: 4664
ApaLI G'TGCA_C
Cuts at: 526 3502 526
Size: 2976 1688
ApoI r'AATT_y
Cuts at: 1668 4017 4273 1668
Size: 2349 256 2059
Fragments arranged by size:
2349 2059 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 385 1330 1612 1684 385
Size: 945 282 72 3365
Fragments arranged by size:
3365 945 282 72
AvrII C'CTAG_G
Cuts at: 117 117
Size: 4664
BamHI G'GATC_C
Cuts at: 1689 1689
Size: 4664
BanI G'GyrC_C
Cuts at: 819 978 1092 1680 2094 2342 2377 819
Size: 159 114 588 414 248 35 3106
Fragments arranged by size:
3106 588 414 248 159 114 35
BanII G_rGCy'C
Cuts at: 1001 1249 1678 2708 1001
Size: 248 429 1030 2957
Fragments arranged by size:
2957 1030 429 248
BbeI G_GCGC'C
Cuts at: 823 982 2098 2346 823
Size: 159 1116 248 3141
Fragments arranged by size:
3141 1116 248 159
BbsI GAAGACnn'nnnn_
Cuts at: 1213 1555 1213
Size: 342 4322
BbvCI CC'TCA_GC
Cuts at: 1182 1182
Size: 4664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 702 736 1342 1376 702
Size: 34 606 34 3990
Fragments arranged by size:
3990 606 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2562 3618 4664 2562
Size: 1056 1046 2562
Fragments arranged by size:
2562 1056 1046
BclI T'GATC_A
Cuts at: 1625 2184 1625
Size: 559 4105
BglI GCCn_nnn'nGGC
Cuts at: 112 514 583 1268 112
Size: 402 69 685 3508
Fragments arranged by size:
3508 685 402 69
BglII A'GATC_T
Cuts at: 2179 4216 2179
Size: 2037 2627
BlpI GC'TnA_GC
Cuts at: 1558 1776 1558
Size: 218 4446
Bme1580I G_kGCm'C
Cuts at: 499 530 1001 2289 2382 3506 499
Size: 31 471 1288 93 1124 1657
Fragments arranged by size:
1657 1288 1124 471 93 31
BmgBI CAC'GTC
Cuts at: 473 1461 473
Size: 988 3676
BmrI ACTGGGnnnn_n'
Cuts at: 486 1876 1957 2292 4436 486
Size: 1390 81 335 2144 714
Fragments arranged by size:
2144 1390 714 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1197 3031 1197
Size: 1834 2830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 757 769 924 268
Size: 489 12 155 4008
Fragments arranged by size:
4008 489 155 12
Bpu10I CC'TnA_GC
Cuts at: 1182 1182
Size: 4664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 355 637 1233 1582 1636 1840 2719 3433
Size: 76 282 596 349 54 204 879 714
Cuts at: 3433 3731 279
Size: 298 1212
Fragments arranged by size:
1212 879 714 596 349 298 282 204
76 54
BsaI GGTCTCn'nnnn_
Cuts at: 4368 4368
Size: 4664
BsaAI yAC'GTr
Cuts at: 230 2017 230
Size: 1787 2877
BsaBI GATnn'nnATC
Cuts at: 6 2178 4215 6
Size: 2172 2037 455
Fragments arranged by size:
2172 2037 455
BsaHI Gr'CG_yC
Cuts at: 820 979 1210 2095 2343 3045 4541 820
Size: 159 231 885 248 702 1496 943
Fragments arranged by size:
1496 943 885 702 248 231 159
BsaWI w'CCGG_w
Cuts at: 1853 2374 3052 3463 3610 3884 1853
Size: 521 678 411 147 274 2633
Fragments arranged by size:
2633 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 742 772 742
Size: 30 4634
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 301 352 595 604 607 618 694 697
Size: 3 51 243 9 3 11 76 3
Cuts at: 697 799 1006 1048 1057 1060 1075 1087 1090
Size: 102 207 42 9 3 15 12 3
Cuts at: 1090 1093 1096 1150 1168 1170 1290 298
Size: 3 3 54 18 2 120 3672
Fragments arranged by size:
3672 243 207 120 102 76 54 51
42 18 15 12 11 9 9 3
3 3 3 3 3 3 2
BseYI C'CCAG_C
Cuts at: 1276 1421 2089 2847 3512 3920 1276
Size: 145 668 758 665 408 2020
Fragments arranged by size:
2020 758 668 665 408 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1483 1483
Size: 4664
BsiEI CG_ry'CG
Cuts at: 104 2206 2252 3482 104
Size: 2102 46 1230 1286
Fragments arranged by size:
2102 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 530 1249 1678 2456 2646 3506 530
Size: 719 429 778 190 860 1688
Fragments arranged by size:
1688 860 778 719 429 190
Bsp1286I G_dGCh'C
Cuts at: 499 530 1001 1249 1678 2289 2382 2456 2646
Size: 31 471 248 429 611 93 74 190
Cuts at: 2646 2708 3506 499
Size: 62 798 1657
Fragments arranged by size:
1657 798 611 471 429 248 190 93
74 62 31
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1455 1532 1714 1928 2230 2611 1455
Size: 77 182 214 302 381 3508
Fragments arranged by size:
3508 381 302 214 182 77
BsrBI CCG'CTC
Cuts at: 50 252 1227 2956 3010 4494 4659 50
Size: 202 975 1729 54 1484 165 55
Fragments arranged by size:
1729 1484 975 202 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2576 2576
Size: 4664
BsrFI r'CCGG_y
Cuts at: 2662 2843 2662
Size: 181 4483
BssSI C'ACGA_G
Cuts at: 2935 3643 2935
Size: 708 3956
BstAPI GCAn_nnn'nTGC
Cuts at: 2041 2041
Size: 4664
BstBI TT'CG_AA
Cuts at: 3025 3025
Size: 4664
BstYI r'GATC_y
Cuts at: 1 1592 1689 2156 2179 2514 2760 3066 3078
Size: 1591 97 467 23 335 246 306 12
Cuts at: 3078 3164 4216 1
Size: 86 1052 449
Fragments arranged by size:
1591 1052 467 449 335 306 246 97
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1058 1058
Size: 4664
BtgI C'CryG_G
Cuts at: 107 107
Size: 4664
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 547 905 2614 2795 2958 547
Size: 358 1709 181 163 2253
Fragments arranged by size:
2253 1709 358 181 163
BtsI GCAGTG_nn'
Cuts at: 318 572 1907 318
Size: 254 1335 3075
Fragments arranged by size:
3075 1335 254
DraI TTT'AAA
Cuts at: 1663 1663
Size: 4664
DraIII CAC_nnn'GTG
Cuts at: 494 494
Size: 4664
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3714 2370
Size: 1344 3320
EaeI y'GGCC_r
Cuts at: 699 827 2249 2423 2814 2841 4186 699
Size: 128 1422 174 391 27 1345 1177
Fragments arranged by size:
1422 1345 1177 391 174 128 27
EagI C'GGCC_G
Cuts at: 2249 2249
Size: 4664
EarI CTCTTCn'nnn_
Cuts at: 950 1195 2687 2897 950
Size: 245 1492 210 2717
Fragments arranged by size:
2717 1492 245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2872 3614 3760 4474 2872
Size: 742 146 714 3062
Fragments arranged by size:
3062 742 714 146
EcoICRI GAG'CTC
Cuts at: 1247 1676 1247
Size: 429 4235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 612 757 769 924 2489 2921 3274 3936
Size: 344 145 12 155 1565 432 353 662
Cuts at: 3936 268
Size: 996
Fragments arranged by size:
1565 996 662 432 353 344 155 145
12
EcoNI CCTnn'n_nnAGG
Cuts at: 1654 1654
Size: 4664
EcoO109I rG'GnC_Cy
Cuts at: 121 506 997 1390 1521 1803 121
Size: 385 491 393 131 282 2982
Fragments arranged by size:
2982 491 393 385 282 131
EcoRI G'AATT_C
Cuts at: 1668 1668
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4664
FspI TGC'GCA
Cuts at: 724 2445 724
Size: 1721 2943
HaeII r_GCGC'y
Cuts at: 823 982 2098 2346 3576 4347 823
Size: 159 1116 248 1230 771 1140
Fragments arranged by size:
1230 1140 1116 771 248 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 457 489 742 774 913 945 2506 2536 2538
Size: 32 253 32 139 32 1561 30 2
Cuts at: 2538 2568 457
Size: 30 2553
Fragments arranged by size:
2553 1561 253 139 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 219 414 1415 1703 219
Size: 195 1001 288 3180
Fragments arranged by size:
3180 1001 288 195
HindIII A'AGCT_T
Cuts at: 328 328
Size: 4664
KasI G'GCGC_C
Cuts at: 819 978 2094 2342 819
Size: 159 1116 248 3141
Fragments arranged by size:
3141 1116 248 159
KpnI G_GTAC'C
Cuts at: 1684 1684
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1121 3423 3607 1121
Size: 2302 184 2178
Fragments arranged by size:
2302 2178 184
MscI TGG'CCA
Cuts at: 829 2425 829
Size: 1596 3068
MslI CAynn'nnrTG
Cuts at: 2653 2780 2653
Size: 127 4537
MspA1I CmG'CkG
Cuts at: 552 645 825 1454 1775 2089 2449 3233 3478
Size: 93 180 629 321 314 360 784 245
Cuts at: 3478 552
Size: 1738
Fragments arranged by size:
1738 784 629 360 321 314 245 180
93
NaeI GCC'GGC
Cuts at: 2845 2845
Size: 4664
NarI GG'CG_CC
Cuts at: 820 979 2095 2343 820
Size: 159 1116 248 3141
Fragments arranged by size:
3141 1116 248 159
NcoI C'CATG_G
Cuts at: 107 107
Size: 4664
NgoMIV G'CCGG_C
Cuts at: 2843 2843
Size: 4664
NspI r_CATG'y
Cuts at: 930 1717 2217 3820 930
Size: 787 500 1603 1774
Fragments arranged by size:
1774 1603 787 500
PciI A'CATG_T
Cuts at: 926 3816 926
Size: 2890 1774
PflMI CCAn_nnn'nTGG
Cuts at: 735 918 735
Size: 183 4481
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 4632
PpuMI rG'GwC_Cy
Cuts at: 1390 1521 1390
Size: 131 4533
PspOMI G'GGCC_C
Cuts at: 997 997
Size: 4664
PspXI vC'TCGA_Gb
Cuts at: 1330 1330
Size: 4664
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 460 492 460
Size: 32 4632
PstI C_TGCA'G
Cuts at: 1365 1711 2396 1365
Size: 346 685 3633
Fragments arranged by size:
3633 685 346
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 2089 2449 2089
Size: 360 4304
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 1249 1678 1249
Size: 429 4235
SalI G'TCGA_C
Cuts at: 1413 1701 1413
Size: 288 4376
SanDI GG'GwC_CC
Cuts at: 1390 1390
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 950 2687 2897 950
Size: 1737 210 2717
Fragments arranged by size:
2717 1737 210
SbfI CC_TGCA'GG
Cuts at: 1711 1711
Size: 4664
ScaI AGT'ACT
Cuts at: 4181 4181
Size: 4664
SfcI C'TryA_G
Cuts at: 33 1361 1707 2392 3360 3551 33
Size: 1328 346 685 968 191 1146
Fragments arranged by size:
1328 1146 968 685 346 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 514 514
Size: 4664
SfoI GGC'GCC
Cuts at: 821 980 2096 2344 821
Size: 159 1116 248 3141
Fragments arranged by size:
3141 1116 248 159
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 1686 1686
Size: 4664
SmlI C'TyrA_G
Cuts at: 258 370 652 1248 1330 1597 1651 1819 2734
Size: 112 282 596 82 267 54 168 915
Cuts at: 2734 3448 3710 258
Size: 714 262 1212
Fragments arranged by size:
1212 915 714 596 282 267 262 168
112 82 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 1717 2217 1717
Size: 500 4164
StuI AGG'CCT
Cuts at: 517 877 1177 517
Size: 360 300 4004
Fragments arranged by size:
4004 360 300
StyI C'CwwG_G
Cuts at: 107 117 803 830 974 1001 1798 2152 107
Size: 10 686 27 144 27 797 354 2619
Fragments arranged by size:
2619 797 686 354 144 27 27 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 505 1110 1230 1461 2247 2380 2839 3048 505
Size: 605 120 231 786 133 459 209 2121
Fragments arranged by size:
2121 786 605 459 231 209 133 120
TatI w'GTAC_w
Cuts at: 4179 4179
Size: 4664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4181 4213 4181
Size: 32 4632
Tth111I GACn'n_nGTC
Cuts at: 214 2461 214
Size: 2247 2417
XbaI T'CTAG_A
Cuts at: 1695 1695
Size: 4664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1402 2159 1402
Size: 757 3907
XhoI C'TCGA_G
Cuts at: 1330 1330
Size: 4664
XmaI C'CCGG_G
Cuts at: 1684 1684
Size: 4664
XmnI GAAnn'nnTTC
Cuts at: 625 1534 625
Size: 909 3755
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI AscI
BaeI BfrBI BmtI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI SacII SexAI
SgrAI SnaBI SrfI SspI SwaI ZraI