Restriction map for
pF1KB9483
(Circular) (Six-base) MAPSORT of: pF1KB9483.seq Check: 6732 from: 1 to: 5684
pF1KB9483 5684 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 2662 2722 2662
Size: 60 5624
Acc65I G'GTAC_C
Cuts at: 2700 2700
Size: 5684
AclI AA'CG_TT
Cuts at: 969 5524 969
Size: 4555 1129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 260 281 1175 1379 1412 1501 1639 3509
Size: 61 21 894 204 33 89 138 1870
Cuts at: 3509 3941 4294 4956 199
Size: 432 353 662 927
Fragments arranged by size:
1870 927 894 662 432 353 204 138
89 61 33 21
AflIII A'CryG_T
Cuts at: 3036 4836 5117 3036
Size: 1800 281 3603
Fragments arranged by size:
3603 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1827 1827
Size: 5684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 473 473
Size: 5684
AlwNI CAG_nnn'CTG
Cuts at: 1527 1617 2989 4427 1527
Size: 90 1372 1438 2784
Fragments arranged by size:
2784 1438 1372 90
ApaI G_GGCC'C
Cuts at: 2447 2447
Size: 5684
ApaLI G'TGCA_C
Cuts at: 4522 4522
Size: 5684
ApoI r'AATT_y
Cuts at: 2061 2688 5037 5293 2061
Size: 627 2349 256 2452
Fragments arranged by size:
2452 2349 627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5684
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5684
AvaI C'yCGr_G
Cuts at: 590 2704 590
Size: 2114 3570
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2418 2451 2418
Size: 33 5651
BamHI G'GATC_C
Cuts at: 2709 2709
Size: 5684
BanI G'GyrC_C
Cuts at: 1201 2700 3114 3362 3397 1201
Size: 1499 414 248 35 3488
Fragments arranged by size:
3488 1499 414 248 35
BanII G_rGCy'C
Cuts at: 552 1734 2447 2540 2698 3728 552
Size: 1182 713 93 158 1030 2508
Fragments arranged by size:
2508 1182 1030 713 158 93
BbeI G_GCGC'C
Cuts at: 3118 3366 3118
Size: 248 5436
BbsI GAAGACnn'nnnn_
Cuts at: 205 205
Size: 5684
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2281 2315 2281
Size: 34 5650
BciVI GTATCCnnnnn_n'
Cuts at: 1674 3582 4638 5684 1674
Size: 1908 1056 1046 1674
Fragments arranged by size:
1908 1674 1056 1046
BclI T'GATC_A
Cuts at: 903 3204 903
Size: 2301 3383
BfrBI ATG'CAT
Cuts at: 2248 2248
Size: 5684
BglII A'GATC_T
Cuts at: 3199 5236 3199
Size: 2037 3647
BlpI GC'TnA_GC
Cuts at: 2796 2796
Size: 5684
Bme1580I G_kGCm'C
Cuts at: 2447 3309 3402 4526 2447
Size: 862 93 1124 3605
Fragments arranged by size:
3605 1124 862 93
BmrI ACTGGGnnnn_n'
Cuts at: 743 2896 2977 3312 5456 743
Size: 2153 81 335 2144 971
Fragments arranged by size:
2153 2144 971 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 681 2408 4051 681
Size: 1727 1643 2314
Fragments arranged by size:
2314 1727 1643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1784 2554 1784
Size: 770 4914
Bpu10I CC'TnA_GC
Cuts at: 446 446
Size: 5684
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2860 3739 4453 4751 2860
Size: 879 714 298 3793
Fragments arranged by size:
3793 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 565 1364 5388 565
Size: 799 4024 861
Fragments arranged by size:
4024 861 799
BsaAI yAC'GTr
Cuts at: 2440 3037 2440
Size: 597 5087
BsaBI GATnn'nnATC
Cuts at: 6 3198 5235 6
Size: 3192 2037 455
Fragments arranged by size:
3192 2037 455
BsaHI Gr'CG_yC
Cuts at: 3115 3363 4065 5561 3115
Size: 248 702 1496 3238
Fragments arranged by size:
3238 1496 702 248
BsaWI w'CCGG_w
Cuts at: 123 2638 2873 3394 4072 4483 4630 4904 123
Size: 2515 235 521 678 411 147 274 903
Fragments arranged by size:
2515 903 678 521 411 274 235 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 412 430 433 436 439 442 445 608
Size: 234 18 3 3 3 3 3 163
Cuts at: 608 635 178
Size: 27 5227
Fragments arranged by size:
5227 234 163 27 18 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 1527 2120 3109 3867 4532 4940 1527
Size: 593 989 758 665 408 2271
Fragments arranged by size:
2271 989 758 665 593 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1224 1589 1224
Size: 365 5319
BsiEI CG_ry'CG
Cuts at: 104 3226 3272 4502 104
Size: 3122 46 1230 1286
Fragments arranged by size:
3122 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 552 1868 2540 2698 3476 3666 4526 552
Size: 1316 672 158 778 190 860 1710
Fragments arranged by size:
1710 1316 860 778 672 190 158
Bsp1286I G_dGCh'C
Cuts at: 552 1734 1868 2447 2540 2698 3309 3402 3476
Size: 1182 134 579 93 158 611 93 74
Cuts at: 3476 3666 3728 4526 552
Size: 190 62 798 1710
Fragments arranged by size:
1710 1182 798 611 579 190 158 134
93 93 74 62
BspEI T'CCGG_A
Cuts at: 123 123
Size: 5684
BspHI T'CATG_A
Cuts at: 4116 4116
Size: 5684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1246 2734 2948 3250 3631 1246
Size: 1488 214 302 381 3299
Fragments arranged by size:
3299 1488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3976 4030 5514 5679 50
Size: 3926 54 1484 165 55
Fragments arranged by size:
3926 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 712 1727 1824 3596 712
Size: 1015 97 1772 2800
Fragments arranged by size:
2800 1772 1015 97
BsrFI r'CCGG_y
Cuts at: 3682 3863 3682
Size: 181 5503
BsrGI T'GTAC_A
Cuts at: 1296 2400 1296
Size: 1104 4580
BssSI C'ACGA_G
Cuts at: 3955 4663 3955
Size: 708 4976
BstAPI GCAn_nnn'nTGC
Cuts at: 1594 1603 1858 3061 1594
Size: 9 255 1203 4217
Fragments arranged by size:
4217 1203 255 9
BstBI TT'CG_AA
Cuts at: 170 4045 170
Size: 3875 1809
BstYI r'GATC_y
Cuts at: 1 2709 3176 3199 3534 3780 4086 4098 4184
Size: 2708 467 23 335 246 306 12 86
Cuts at: 4184 5236 1
Size: 1052 449
Fragments arranged by size:
2708 1052 467 449 335 306 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 2663 2663
Size: 5684
Bsu36I CC'TnA_GG
Cuts at: 1425 1425
Size: 5684
BtgI C'CryG_G
Cuts at: 917 1082 2475 917
Size: 165 1393 4126
Fragments arranged by size:
4126 1393 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 479 2161 3634 3815 3978 479
Size: 1682 1473 181 163 2185
Fragments arranged by size:
2185 1682 1473 181 163
BtsI GCAGTG_nn'
Cuts at: 455 2634 2927 455
Size: 2179 293 3212
Fragments arranged by size:
3212 2179 293
ClaI AT'CG_AT
Cuts at: 2424 2424
Size: 5684
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1469 1504 1469
Size: 35 5649
DraI TTT'AAA
Cuts at: 743 941 1418 1712 2683 743
Size: 198 477 294 971 3744
Fragments arranged by size:
3744 971 477 294 198
DrdI GACnn_nn'nnGTC
Cuts at: 310 3390 4734 310
Size: 3080 1344 1260
Fragments arranged by size:
3080 1344 1260
EaeI y'GGCC_r
Cuts at: 914 3269 3443 3834 3861 5206 914
Size: 2355 174 391 27 1345 1392
Fragments arranged by size:
2355 1392 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3269 3269
Size: 5684
EarI CTCTTCn'nnn_
Cuts at: 179 398 407 599 1205 2583 3707 3917 179
Size: 219 9 192 606 1378 1124 210 1946
Fragments arranged by size:
1946 1378 1124 606 219 210 192 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 192 3892 4634 4780 5494 192
Size: 3700 742 146 714 382
Fragments arranged by size:
3700 742 714 382 146
EcoICRI GAG'CTC
Cuts at: 550 2538 2696 550
Size: 1988 158 3538
Fragments arranged by size:
3538 1988 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 260 281 1175 1379 1412 1501 1639 1784
Size: 61 21 894 204 33 89 138 145
Cuts at: 1784 2554 3509 3941 4294 4956 199
Size: 770 955 432 353 662 927
Fragments arranged by size:
955 927 894 770 662 432 353 204
145 138 89 61 33 21
EcoO109I rG'GnC_Cy
Cuts at: 1071 2444 2529 2823 1071
Size: 1373 85 294 3932
Fragments arranged by size:
3932 1373 294 85
EcoRI G'AATT_C
Cuts at: 2688 2688
Size: 5684
EcoRV GAT'ATC
Cuts at: 1269 1748 1269
Size: 479 5205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3703 3703
Size: 5684
FspI TGC'GCA
Cuts at: 737 3465 737
Size: 2728 2956
HaeII r_GCGC'y
Cuts at: 3118 3366 4596 5367 3118
Size: 248 1230 771 3435
Fragments arranged by size:
3435 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 888 920 1792 1813 1824 1845 3526 3556 3558
Size: 32 872 21 11 21 1681 30 2
Cuts at: 3558 3588 888
Size: 30 2984
Fragments arranged by size:
2984 1681 872 32 30 30 21 21
11 2
HincII GTy'rAC
Cuts at: 1095 2723 1095
Size: 1628 4056
HindIII A'AGCT_T
Cuts at: 1052 1139 1394 1052
Size: 87 255 5342
Fragments arranged by size:
5342 255 87
HpaI GTT'AAC
Cuts at: 1095 1095
Size: 5684
KasI G'GCGC_C
Cuts at: 3114 3362 3114
Size: 248 5436
KpnI G_GTAC'C
Cuts at: 2704 2704
Size: 5684
MluI A'CGCG_T
Cuts at: 5117 5117
Size: 5684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4443 4627 4443
Size: 184 5500
MscI TGG'CCA
Cuts at: 916 3445 916
Size: 2529 3155
MslI CAynn'nnrTG
Cuts at: 1490 3673 3800 1490
Size: 2183 127 3374
Fragments arranged by size:
3374 2183 127
MspA1I CmG'CkG
Cuts at: 238 981 1105 1527 2795 3109 3469 4253 4498
Size: 743 124 422 1268 314 360 784 245
Cuts at: 4498 238
Size: 1424
Fragments arranged by size:
1424 1268 784 743 422 360 314 245
124
NaeI GCC'GGC
Cuts at: 3865 3865
Size: 5684
NarI GG'CG_CC
Cuts at: 3115 3363 3115
Size: 248 5436
NcoI C'CATG_G
Cuts at: 917 1082 917
Size: 165 5519
NdeI CA'TA_TG
Cuts at: 2357 2357
Size: 5684
NgoMIV G'CCGG_C
Cuts at: 3863 3863
Size: 5684
NsiI A_TGCA'T
Cuts at: 2250 2250
Size: 5684
NspI r_CATG'y
Cuts at: 2275 2392 2737 3237 4840 2275
Size: 117 345 500 1603 3119
Fragments arranged by size:
3119 1603 500 345 117
PciI A'CATG_T
Cuts at: 4836 4836
Size: 5684
PflMI CCAn_nnn'nTGG
Cuts at: 961 961
Size: 5684
PmeI GTTT'AAAC
Cuts at: 2683 2683
Size: 5684
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 4130
Size: 32 5652
PspOMI G'GGCC_C
Cuts at: 2443 2443
Size: 5684
PspXI vC'TCGA_Gb
Cuts at: 590 590
Size: 5684
PstI C_TGCA'G
Cuts at: 2731 3416 2731
Size: 685 4999
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5684
PvuII CAG'CTG
Cuts at: 238 981 1105 1527 3109 3469 238
Size: 743 124 422 1582 360 2453
Fragments arranged by size:
2453 1582 743 422 360 124
RsrII CG'GwC_CG
Cuts at: 3879 3879
Size: 5684
SacI G_AGCT'C
Cuts at: 552 2540 2698 552
Size: 1988 158 3538
Fragments arranged by size:
3538 1988 158
SalI G'TCGA_C
Cuts at: 2721 2721
Size: 5684
SapI GCTCTTCn'nnn_
Cuts at: 3707 3917 3707
Size: 210 5474
SbfI CC_TGCA'GG
Cuts at: 2731 2731
Size: 5684
ScaI AGT'ACT
Cuts at: 5201 5201
Size: 5684
SfcI C'TryA_G
Cuts at: 33 2325 2675 2727 3412 4380 4571 33
Size: 2292 350 52 685 968 191 1146
Fragments arranged by size:
2292 1146 968 685 350 191 52
SfoI GGC'GCC
Cuts at: 3116 3364 3116
Size: 248 5436
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5684
SmaI CCC'GGG
Cuts at: 2706 2706
Size: 5684
SmlI C'TyrA_G
Cuts at: 590 2839 3754 4468 4730 590
Size: 2249 915 714 262 1544
Fragments arranged by size:
2249 1544 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1341 67
Size: 1274 4410
SphI G_CATG'C
Cuts at: 2275 2737 3237 2275
Size: 462 500 4722
Fragments arranged by size:
4722 500 462
SspI AAT'ATT
Cuts at: 804 1856 1984 804
Size: 1052 128 4504
Fragments arranged by size:
4504 1052 128
StuI AGG'CCT
Cuts at: 528 1423 2342 528
Size: 895 919 3870
Fragments arranged by size:
3870 919 895
StyI C'CwwG_G
Cuts at: 693 917 1082 2818 3172 693
Size: 224 165 1736 354 3205
Fragments arranged by size:
3205 1736 354 224 165
SwaI ATTT'AAAT
Cuts at: 743 743
Size: 5684
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 556 889 3267 3400 3859 4068 556
Size: 333 2378 133 459 209 2172
Fragments arranged by size:
2378 2172 459 333 209 133
TatI w'GTAC_w
Cuts at: 1296 2400 5199 1296
Size: 1104 2799 1781
Fragments arranged by size:
2799 1781 1104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1879 1911 2273 2305 5201 5233 1879
Size: 32 362 32 2896 32 2330
Fragments arranged by size:
2896 2330 362 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3481 3481
Size: 5684
XbaI T'CTAG_A
Cuts at: 2715 2715
Size: 5684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3179 3179
Size: 5684
XhoI C'TCGA_G
Cuts at: 590 590
Size: 5684
XmaI C'CCGG_G
Cuts at: 2704 2704
Size: 5684
XmnI GAAnn'nnTTC
Cuts at: 1397 2134 1397
Size: 737 4947
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI AscI
AvrII BbvCI BglI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BssHII BstEII BstXI DraIII EcoNI FseI FspAI
MfeI NheI NotI NruI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI ZraI