Restriction map for
pF1KB9484
(Circular) (Six-base) MAPSORT of: pF1KB9484.seq Check: 1559 from: 1 to: 9248
pF1KB9484 9248 bp
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4177 5517 5897 5997 6200 4177
Size: 1340 380 100 203 7225
Fragments arranged by size:
7225 1340 380 203 100
AatII G_ACGT'C
Cuts at: 3653 4071 4682 5537 3653
Size: 418 611 855 7364
Fragments arranged by size:
7364 855 611 418
AccI GT'mk_AC
Cuts at: 6286 6286
Size: 9248
Acc65I G'GTAC_C
Cuts at: 6264 6264
Size: 9248
AclI AA'CG_TT
Cuts at: 9088 9088
Size: 9248
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2211 2320 2977 3732 5403 5404 7073 7505 8252
Size: 109 657 755 1671 1 1669 432 747
Cuts at: 8252 8520 2211
Size: 268 2939
Fragments arranged by size:
2939 1671 1669 755 747 657 432 268
109 1
AflIII A'CryG_T
Cuts at: 293 1540 1823 4027 6600 7704 8681 293
Size: 1247 283 2204 2573 1104 977 860
Fragments arranged by size:
2573 2204 1247 1104 977 860 283
AgeI A'CCGG_T
Cuts at: 7636 7636
Size: 9248
AhdI GACnn_n'nnGTC
Cuts at: 3579 5178 5538 3579
Size: 1599 360 7289
Fragments arranged by size:
7289 1599 360
AleI CACnn'nnGTG
Cuts at: 595 3502 595
Size: 2907 6341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 829 1475 2112 4687 5084 829
Size: 646 637 2575 397 4993
Fragments arranged by size:
4993 2575 646 637 397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 541 573 5092 5124 541
Size: 32 4519 32 4665
Fragments arranged by size:
4665 4519 32 32
AlwNI CAG_nnn'CTG
Cuts at: 881 1002 2033 2163 2483 2982 3003 3096 3549
Size: 121 1031 130 320 499 21 93 453
Cuts at: 3549 4329 4695 4806 5502 5820 6553 8120 881
Size: 780 366 111 696 318 733 1567 2009
Fragments arranged by size:
2009 1567 1031 780 733 696 499 453
366 320 318 130 121 111 93 21
ApaI G_GGCC'C
Cuts at: 133 1486 2770 3010 3695 3774 3972 4794 5012
Size: 1353 1284 240 685 79 198 822 218
Cuts at: 5012 6115 133
Size: 1103 3266
Fragments arranged by size:
3266 1353 1284 1103 822 685 240 218
198 79
ApaLI G'TGCA_C
Cuts at: 1852 3724 8018 1852
Size: 1872 4294 3082
Fragments arranged by size:
4294 3082 1872
ApoI r'AATT_y
Cuts at: 3642 4303 6252 8601 8857 3642
Size: 661 1949 2349 256 4033
Fragments arranged by size:
4033 2349 1949 661 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9248
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9248
AvaI C'yCGr_G
Cuts at: 126 350 577 946 1086 1144 1235 1513 1590
Size: 224 227 369 140 58 91 278 77
Cuts at: 1590 1672 2084 2450 2716 3361 3562 3767 3965
Size: 82 412 366 266 645 201 205 198
Cuts at: 3965 4352 4603 4727 4927 4989 5564 5607 5638
Size: 387 251 124 200 62 575 43 31
Cuts at: 5638 6268 126
Size: 630 3106
Fragments arranged by size:
3106 645 630 575 412 387 369 366
278 266 251 227 224 205 201 200
198 140 124 91 82 77 62 58
43 31
BamHI G'GATC_C
Cuts at: 6273 6273
Size: 9248
BanI G'GyrC_C
Cuts at: 646 726 940 973 1072 1121 1167 2515 2665
Size: 80 214 33 99 49 46 1348 150
Cuts at: 2665 3112 3228 3240 3753 3800 4195 4219 4469
Size: 447 116 12 513 47 395 24 250
Cuts at: 4469 4496 4504 4555 4645 4908 4969 5314 5464
Size: 27 8 51 90 263 61 345 150
Cuts at: 5464 5885 6049 6179 6264 6678 6926 6961 646
Size: 421 164 130 85 414 248 35 2933
Fragments arranged by size:
2933 1348 513 447 421 414 395 345
263 250 248 214 164 150 150 130
116 99 90 85 80 61 51 49
47 46 35 33 27 24 12 8
BanII G_rGCy'C
Cuts at: 133 662 709 1301 1486 1740 2333 2770 3010
Size: 529 47 592 185 254 593 437 240
Cuts at: 3010 3080 3362 3368 3484 3695 3774 3972 4794
Size: 70 282 6 116 211 79 198 822
Cuts at: 4794 4879 5012 5914 6078 6115 6262 7292 133
Size: 85 133 902 164 37 147 1030 2089
Fragments arranged by size:
2089 1030 902 822 593 592 529 437
282 254 240 211 198 185 164 147
133 116 85 79 70 47 37 6
BbeI G_GCGC'C
Cuts at: 650 730 944 977 1076 1125 1171 2669 3116
Size: 80 214 33 99 49 46 1498 447
Cuts at: 3116 3804 4199 4223 4500 4649 4912 6053 6682
Size: 688 395 24 277 149 263 1141 629
Cuts at: 6682 6930 650
Size: 248 2968
Fragments arranged by size:
2968 1498 1141 688 629 447 395 277
263 248 214 149 99 80 49 46
33 24
BbsI GAAGACnn'nnnn_
Cuts at: 1026 2965 3463 5161 1026
Size: 1939 498 1698 5113
Fragments arranged by size:
5113 1939 1698 498
BbvCI CC'TCA_GC
Cuts at: 1890 1890
Size: 9248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 225 259 1271 1305 4918 4952 225
Size: 34 1012 34 3613 34 4521
Fragments arranged by size:
4521 3613 1012 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5694 7146 7907 9248 5694
Size: 1452 761 1341 5694
Fragments arranged by size:
5694 1452 1341 761
BclI T'GATC_A
Cuts at: 386 6768 386
Size: 6382 2866
BglI GCCn_nnn'nGGC
Cuts at: 1165 2468 2633 2774 2978 3751 4184 4326 4841
Size: 1303 165 141 204 773 433 142 515
Cuts at: 4841 5129 1165
Size: 288 5284
Fragments arranged by size:
5284 1303 773 515 433 288 204 165
142 141
BglII A'GATC_T
Cuts at: 6763 8800 6763
Size: 2037 7211
BlpI GC'TnA_GC
Cuts at: 817 2859 6360 817
Size: 2042 3501 3705
Fragments arranged by size:
3705 3501 2042
Bme1580I G_kGCm'C
Cuts at: 133 715 925 1294 1398 1486 1774 1823 1856
Size: 582 210 369 104 88 288 49 33
Cuts at: 1856 2409 2763 2770 3010 3233 3695 3728 3756
Size: 553 354 7 240 223 462 33 28
Cuts at: 3756 3774 3972 4064 4507 4794 4837 4954 4972
Size: 18 198 92 443 287 43 117 18
Cuts at: 4972 5012 5319 5888 5945 6115 6873 6966 8022
Size: 40 307 569 57 170 758 93 1056
Cuts at: 8022 133
Size: 1359
Fragments arranged by size:
1359 1056 758 582 569 553 462 443
369 354 307 288 287 240 223 210
198 170 117 104 93 92 88 57
49 43 40 33 33 28 18 18
7
BmgBI CAC'GTC
Cuts at: 1541 3762 3890 4030 5540 5697 1541
Size: 2221 128 140 1510 157 5092
Fragments arranged by size:
5092 2221 1510 157 140 128
BmrI ACTGGGnnnn_n'
Cuts at: 443 2471 3974 4155 4544 6235 6460 6541 6876
Size: 2028 1503 181 389 1691 225 81 335
Cuts at: 6876 9020 443
Size: 2144 671
Fragments arranged by size:
2144 2028 1691 1503 671 389 335 225
181 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 424 2874 4263 7615 424
Size: 2450 1389 3352 2057
Fragments arranged by size:
3352 2450 2057 1389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 498 565 578 1672 2044 3541 3547 4336
Size: 263 67 13 1094 372 1497 6 789
Cuts at: 4336 4498 4648 4717 4828 5065 5119 5227 5314
Size: 162 150 69 111 237 54 108 87
Cuts at: 5314 5850 6217 235
Size: 536 367 3266
Fragments arranged by size:
3266 1497 1094 789 536 372 367 263
237 162 150 111 108 87 69 67
54 13 6
Bpu10I CC'TnA_GC
Cuts at: 1890 3085 4402 1890
Size: 1195 1317 6736
Fragments arranged by size:
6736 1317 1195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6424 7303 7795 8093 6424
Size: 879 492 298 7579
Fragments arranged by size:
7579 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 395 1246 1277 1747 1900 4519 5292 5484 8952
Size: 851 31 470 153 2619 773 192 3468
Cuts at: 8952 395
Size: 691
Fragments arranged by size:
3468 2619 851 773 691 470 192 153
31
BsaAI yAC'GTr
Cuts at: 296 1412 1826 1851 1884 2337 3840 5400 5517
Size: 1116 414 25 33 453 1503 1560 117
Cuts at: 5517 6601 296
Size: 1084 2943
Fragments arranged by size:
2943 1560 1503 1116 1084 453 414 117
33 25
BsaBI GATnn'nnATC
Cuts at: 6 6762 8799 6
Size: 6756 2037 455
Fragments arranged by size:
6756 2037 455
BsaHI Gr'CG_yC
Cuts at: 607 647 685 727 746 941 974 1073 1122
Size: 40 38 42 19 195 33 99 49
Cuts at: 1122 1168 1625 2413 2666 3113 3268 3460 3515
Size: 46 457 788 253 447 155 192 55
Cuts at: 3515 3533 3650 3801 4068 4196 4220 4497 4646
Size: 18 117 151 267 128 24 277 149
Cuts at: 4646 4679 4909 5534 6050 6203 6679 6927 7629
Size: 33 230 625 516 153 476 248 702
Cuts at: 7629 9125 607
Size: 1496 730
Fragments arranged by size:
1496 788 730 702 625 516 476 457
447 277 267 253 248 230 195 192
155 153 151 149 128 117 99 55
49 46 42 40 38 33 33 24
19 18
BsaWI w'CCGG_w
Cuts at: 2119 3638 3665 6437 6958 7636 7910 8057 8468
Size: 1519 27 2772 521 678 274 147 411
Cuts at: 8468 2119
Size: 2899
Fragments arranged by size:
2899 2772 1519 678 521 411 274 147
27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 283 313 541 571 835 865 1907 1937 2622
Size: 30 228 30 264 30 1042 30 685
Cuts at: 2622 2652 3144 3174 4184 4214 5020 5050 5092
Size: 30 492 30 1010 30 806 30 42
Cuts at: 5092 5122 5896 5926 283
Size: 30 774 30 3605
Fragments arranged by size:
3605 1042 1010 806 774 685 492 264
228 42 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 244 277 562 1234 2092 2863 3070 6027
Size: 3 33 285 672 858 771 207 2957
Cuts at: 6027 241
Size: 3462
Fragments arranged by size:
3462 2957 858 771 672 285 207 33
3
BseYI C'CCAG_C
Cuts at: 715 1468 1796 1831 2526 3031 3130 3256 4051
Size: 753 328 35 695 505 99 126 795
Cuts at: 4051 4085 5125 5915 6045 6114 6673 7431 8008
Size: 34 1040 790 130 69 559 758 577
Cuts at: 8008 8504 715
Size: 496 1459
Fragments arranged by size:
1459 1040 795 790 758 753 695 577
559 505 496 328 130 126 99 69
35 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 449 810 1540 1831 1845 1864 3236 4417
Size: 142 361 730 291 14 19 1372 1181
Cuts at: 4417 5044 5544 6172 307
Size: 627 500 628 3383
Fragments arranged by size:
3383 1372 1181 730 628 627 500 361
291 142 19 14
BsiEI CG_ry'CG
Cuts at: 104 117 142 896 1225 1359 2757 3826 4845
Size: 13 25 754 329 134 1398 1069 1019
Cuts at: 4845 5307 6790 6836 8044 104
Size: 462 1483 46 1208 1308
Fragments arranged by size:
1483 1398 1308 1208 1069 1019 754 462
329 134 46 25 13
BsiHKAI G_wGCw'C
Cuts at: 248 1591 1856 1948 1982 2545 2707 3622 3728
Size: 1343 265 92 34 563 162 915 106
Cuts at: 3728 3839 4941 5516 5828 6262 7040 7230 8022
Size: 111 1102 575 312 434 778 190 792
Cuts at: 8022 248
Size: 1474
Fragments arranged by size:
1474 1343 1102 915 792 778 575 563
434 312 265 190 162 111 106 92
34
BsmI GAATG_Cn'
Cuts at: 635 772 1579 1786 635
Size: 137 807 207 8097
Fragments arranged by size:
8097 807 207 137
Bsp1286I G_dGCh'C
Cuts at: 133 248 662 709 715 925 1294 1301 1398
Size: 115 414 47 6 210 369 7 97
Cuts at: 1398 1486 1591 1740 1774 1823 1856 1948 1982
Size: 88 105 149 34 49 33 92 34
Cuts at: 1982 2333 2409 2545 2707 2763 2770 3010 3080
Size: 351 76 136 162 56 7 240 70
Cuts at: 3080 3233 3362 3368 3484 3622 3695 3728 3756
Size: 153 129 6 116 138 73 33 28
Cuts at: 3756 3774 3839 3972 4064 4507 4794 4837 4879
Size: 18 65 133 92 443 287 43 42
Cuts at: 4879 4941 4954 4972 5012 5319 5516 5828 5888
Size: 62 13 18 40 307 197 312 60
Cuts at: 5888 5914 5945 6078 6115 6262 6873 6966 7040
Size: 26 31 133 37 147 611 93 74
Cuts at: 7040 7230 7292 8022 133
Size: 190 62 730 1359
Fragments arranged by size:
1359 730 611 443 414 369 351 312
307 287 240 210 197 190 162 153
149 147 138 136 133 133 129 116
115 105 97 93 92 92 88 76
74 73 70 65 62 62 60 56
49 47 43 42 40 37 34 34
33 33 31 28 26 18 18 13
7 7 6 6
BspEI T'CCGG_A
Cuts at: 2119 3638 3665 8468 2119
Size: 1519 27 4803 2899
Fragments arranged by size:
4803 2899 1519 27
BspHI T'CATG_A
Cuts at: 8424 8424
Size: 9248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 871 940 4144 4177 5517 5539 5897 5997 6200
Size: 69 3204 33 1340 22 358 100 203
Cuts at: 6200 6298 6512 6814 7195 871
Size: 98 214 302 381 2924
Fragments arranged by size:
3204 2924 1340 381 358 302 214 203
100 98 69 33 22
BsrBI CCG'CTC
Cuts at: 50 153 219 3323 4247 4632 5394 5850 7540
Size: 103 66 3104 924 385 762 456 1690
Cuts at: 7540 7594 9078 9243 50
Size: 54 1484 165 55
Fragments arranged by size:
3104 1690 1484 924 762 456 385 165
103 66 55 54
BsrDI GCAATG_nn'
Cuts at: 3899 4824 6021 7160 3899
Size: 925 1197 1139 5987
Fragments arranged by size:
5987 1197 1139 925
BsrFI r'CCGG_y
Cuts at: 112 116 187 1304 1448 2168 3099 3803 4230
Size: 4 71 1117 144 720 931 704 427
Cuts at: 4230 4425 4449 4648 6031 6176 7246 7427 7636
Size: 195 24 199 1383 145 1070 181 209
Cuts at: 7636 112
Size: 1724
Fragments arranged by size:
1724 1383 1117 1070 931 720 704 427
209 199 195 181 145 144 71 24
4
BsrGI T'GTAC_A
Cuts at: 2638 4337 2638
Size: 1699 7549
BssHII G'CGCG_C
Cuts at: 207 209 886 1693 207
Size: 2 677 807 7762
Fragments arranged by size:
7762 807 677 2
BssSI C'ACGA_G
Cuts at: 912 2706 5117 7519 7877 912
Size: 1794 2411 2402 358 2283
Fragments arranged by size:
2411 2402 2283 1794 358
BstAPI GCAn_nnn'nTGC
Cuts at: 767 1257 1643 2033 4976 5520 6186 6625 767
Size: 490 386 390 2943 544 666 439 3390
Fragments arranged by size:
3390 2943 666 544 490 439 390 386
BstBI TT'CG_AA
Cuts at: 7609 7609
Size: 9248
BstEII G'GTnAC_C
Cuts at: 2274 5551 2274
Size: 3277 5971
BstXI CCAn_nnnn'nTGG
Cuts at: 479 723 3264 5475 6060 479
Size: 244 2541 2211 585 3667
Fragments arranged by size:
3667 2541 2211 585 244
BstYI r'GATC_y
Cuts at: 1 266 446 1682 3785 6273 6740 6763 7098
Size: 265 180 1236 2103 2488 467 23 335
Cuts at: 7098 7344 8356 8442 8454 8800 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2488 2103 1236 1012 467 449 346 335
265 246 180 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2569 4322 4654 5571 2569
Size: 1753 332 917 6246
Fragments arranged by size:
6246 1753 917 332
BtgI C'CryG_G
Cuts at: 107 822 936 1332 1658 2499 2771 3236 3914
Size: 715 114 396 326 841 272 465 678
Cuts at: 3914 4010 4125 4619 4782 4899 5843 5921 6107
Size: 96 115 494 163 117 944 78 186
Cuts at: 6107 107
Size: 3248
Fragments arranged by size:
3248 944 841 715 678 494 465 396
326 272 186 163 117 115 114 96
78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2949 3155 3323 3920 7198 7379 7542 2949
Size: 206 168 597 3278 181 163 4655
Fragments arranged by size:
4655 3278 597 206 181 168 163
BtsI GCAGTG_nn'
Cuts at: 793 1528 1729 1963 2034 2749 3094 3185 3929
Size: 735 201 234 71 715 345 91 744
Cuts at: 3929 4043 5188 5658 5767 6118 6491 793
Size: 114 1145 470 109 351 373 3550
Fragments arranged by size:
3550 1145 744 735 715 470 373 351
345 234 201 114 109 91 71
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2898 2933 2898
Size: 35 9213
DraI TTT'AAA
Cuts at: 6247 6247
Size: 9248
DraIII CAC_nnn'GTG
Cuts at: 2291 3212 2291
Size: 921 8327
DrdI GACnn_nn'nnGTC
Cuts at: 1189 6954 7812 1189
Size: 5765 858 2625
Fragments arranged by size:
5765 2625 858
EaeI y'GGCC_r
Cuts at: 110 114 139 164 481 893 1266 1335 2625
Size: 4 25 25 317 412 373 69 1290
Cuts at: 2625 2754 3386 3823 3917 4389 4842 5304 5870
Size: 129 632 437 94 472 453 462 566
Cuts at: 5870 6833 7007 7398 7425 8770 110
Size: 963 174 391 27 1345 588
Fragments arranged by size:
1345 1290 963 632 588 566 472 462
453 437 412 391 373 317 174 129
94 69 27 25 25 4
EagI C'GGCC_G
Cuts at: 114 139 893 2754 3823 4842 5304 6833 114
Size: 25 754 1861 1069 1019 462 1529 2529
Fragments arranged by size:
2529 1861 1529 1069 1019 754 462 25
EarI CTCTTCn'nnn_
Cuts at: 697 3640 4867 7271 7481 697
Size: 2943 1227 2404 210 2464
Fragments arranged by size:
2943 2464 2404 1227 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 799 3152 3261 5736 7456 7766 7912 9058 799
Size: 2353 109 2475 1720 310 146 1146 989
Fragments arranged by size:
2475 2353 1720 1146 989 310 146 109
EcoICRI GAG'CTC
Cuts at: 6260 6260
Size: 9248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 498 565 578 1672 2044 2211 2320 2977
Size: 263 67 13 1094 372 167 109 657
Cuts at: 2977 3541 3547 3732 4336 4498 4648 4717 4828
Size: 564 6 185 604 162 150 69 111
Cuts at: 4828 5065 5119 5227 5314 5403 5404 5850 6217
Size: 237 54 108 87 89 1 446 367
Cuts at: 6217 7073 7505 8252 8520 235
Size: 856 432 747 268 963
Fragments arranged by size:
1094 963 856 747 657 604 564 446
432 372 367 268 263 237 185 167
162 150 111 109 108 89 87 69
67 54 13 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 547 1744 3088 5227 5280 6196 547
Size: 1197 1344 2139 53 916 3599
Fragments arranged by size:
3599 2139 1344 1197 916 53
EcoO109I rG'GnC_Cy
Cuts at: 581 843 1376 1482 1497 1702 2362 2565 2767
Size: 262 533 106 15 205 660 203 202
Cuts at: 2767 2979 3684 3691 3771 3969 4133 4790 4791
Size: 212 705 7 80 198 164 657 1
Cuts at: 4791 5009 5076 5122 5594 6021 6090 6387 581
Size: 218 67 46 472 427 69 297 3442
Fragments arranged by size:
3442 705 660 657 533 472 427 297
262 218 212 205 203 202 198 164
106 80 69 67 46 15 7 1
EcoRI G'AATT_C
Cuts at: 3642 4303 6252 3642
Size: 661 1949 6638
Fragments arranged by size:
6638 1949 661
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5190 7267 5190
Size: 2077 7171
FseI GG_CCGG'CC
Cuts at: 116 116
Size: 9248
FspI TGC'GCA
Cuts at: 5800 7029 5800
Size: 1229 8019
HaeII r_GCGC'y
Cuts at: 650 730 804 944 977 1076 1125 1171 1764
Size: 80 74 140 33 99 49 46 593
Cuts at: 1764 2669 3116 3804 4199 4223 4315 4381 4500
Size: 905 447 688 395 24 92 66 119
Cuts at: 4500 4627 4649 4695 4912 5098 5411 6053 6682
Size: 127 22 46 217 186 313 642 629
Cuts at: 6682 6930 7952 8931 650
Size: 248 1022 979 967
Fragments arranged by size:
1022 979 967 905 688 642 629 593
447 395 313 248 217 186 140 127
119 99 92 80 74 66 49 46
46 33 24 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 835 867 2182 2214 2600 2632 3144 3176 3565
Size: 32 1315 32 386 32 512 32 389
Cuts at: 3565 3597 4184 4216 5164 5196 5524 5556 7090
Size: 32 587 32 948 32 328 32 1534
Cuts at: 7090 7120 7122 7152 835
Size: 30 2 30 2931
Fragments arranged by size:
2931 1534 1315 948 587 512 389 386
328 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 4683 5949 6287 4683
Size: 1266 338 7644
Fragments arranged by size:
7644 1266 338
KasI G'GCGC_C
Cuts at: 646 726 940 973 1072 1121 1167 2665 3112
Size: 80 214 33 99 49 46 1498 447
Cuts at: 3112 3800 4195 4219 4496 4645 4908 6049 6678
Size: 688 395 24 277 149 263 1141 629
Cuts at: 6678 6926 646
Size: 248 2968
Fragments arranged by size:
2968 1498 1141 688 629 447 395 277
263 248 214 149 99 80 49 46
33 24
KpnI G_GTAC'C
Cuts at: 6268 6268
Size: 9248
MluI A'CGCG_T
Cuts at: 8681 8681
Size: 9248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 628 722 878 1838 3509 4830 7919 8103 628
Size: 94 156 960 1671 1321 3089 184 1773
Fragments arranged by size:
3089 1773 1671 1321 960 184 156 94
MscI TGG'CCA
Cuts at: 483 3388 5872 7009 483
Size: 2905 2484 1137 2722
Fragments arranged by size:
2905 2722 2484 1137
MslI CAynn'nnrTG
Cuts at: 477 595 3502 7237 7364 477
Size: 118 2907 3735 127 2361
Fragments arranged by size:
3735 2907 2361 127 118
MspA1I CmG'CkG
Cuts at: 135 144 392 780 824 1097 1346 1715 1894
Size: 9 248 388 44 273 249 369 179
Cuts at: 1894 2231 2379 2582 2651 2773 2955 3828 4175
Size: 337 148 203 69 122 182 873 347
Cuts at: 4175 5129 5239 5475 5499 5689 5960 6045 6060
Size: 954 110 236 24 190 271 85 15
Cuts at: 6060 6186 6359 6673 7033 8046 8291 135
Size: 126 173 314 360 1013 245 1092
Fragments arranged by size:
1092 1013 954 873 388 369 360 347
337 314 273 271 249 248 245 236
203 190 182 179 173 148 126 122
110 85 69 44 24 15 9
NaeI GCC'GGC
Cuts at: 114 189 1450 2170 3101 4232 4427 4650 6178
Size: 75 1261 720 931 1131 195 223 1528
Cuts at: 6178 7429 114
Size: 1251 1933
Fragments arranged by size:
1933 1528 1261 1251 1131 931 720 223
195 75
NarI GG'CG_CC
Cuts at: 647 727 941 974 1073 1122 1168 2666 3113
Size: 80 214 33 99 49 46 1498 447
Cuts at: 3113 3801 4196 4220 4497 4646 4909 6050 6679
Size: 688 395 24 277 149 263 1141 629
Cuts at: 6679 6927 647
Size: 248 2968
Fragments arranged by size:
2968 1498 1141 688 629 447 395 277
263 248 214 149 99 80 49 46
33 24
NcoI C'CATG_G
Cuts at: 107 2499 4782 4899 107
Size: 2392 2283 117 4456
Fragments arranged by size:
4456 2392 2283 117
NgoMIV G'CCGG_C
Cuts at: 112 187 1448 2168 3099 4230 4425 4648 6176
Size: 75 1261 720 931 1131 195 223 1528
Cuts at: 6176 7427 112
Size: 1251 1933
Fragments arranged by size:
1933 1528 1261 1251 1131 931 720 223
195 75
NotI GC'GGCC_GC
Cuts at: 139 3823 139
Size: 3684 5564
NruI TCG'CGA
Cuts at: 3174 3174
Size: 9248
NspI r_CATG'y
Cuts at: 200 1845 6301 6801 7708 200
Size: 1645 4456 500 907 1740
Fragments arranged by size:
4456 1740 1645 907 500
PasI CC'CwG_GG
Cuts at: 2346 2763 3073 3394 4956 5268 2346
Size: 417 310 321 1562 312 6326
Fragments arranged by size:
6326 1562 417 321 312 310
PciI A'CATG_T
Cuts at: 7704 7704
Size: 9248
PflMI CCAn_nnn'nTGG
Cuts at: 1177 3003 3212 4695 4806 5922 5960 1177
Size: 1826 209 1483 111 1116 38 4465
Fragments arranged by size:
4465 1826 1483 1116 209 111 38
PfoI T'CCnGG_A
Cuts at: 3518 3518
Size: 9248
PmeI GTTT'AAAC
Cuts at: 6247 6247
Size: 9248
PmlI CAC'GTG
Cuts at: 1851 1884 3840 5517 1851
Size: 33 1956 1677 5582
Fragments arranged by size:
5582 1956 1677 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 541 573 5092 5124 5982 6014 8387 8419 541
Size: 32 4519 32 858 32 2373 32 1370
Fragments arranged by size:
4519 2373 1370 858 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 581 843 1702 3684 4133 5076 581
Size: 262 859 1982 449 943 4753
Fragments arranged by size:
4753 1982 943 859 449 262
PshAI GACnn'nnGTC
Cuts at: 1064 5284 5728 1064
Size: 4220 444 4584
Fragments arranged by size:
4584 4220 444
PspOMI G'GGCC_C
Cuts at: 129 1482 2766 3006 3691 3770 3968 4790 5008
Size: 1353 1284 240 685 79 198 822 218
Cuts at: 5008 6111 129
Size: 1103 3266
Fragments arranged by size:
3266 1353 1284 1103 822 685 240 218
198 79
PspXI vC'TCGA_Gb
Cuts at: 1590 3361 1590
Size: 1771 7477
PstI C_TGCA'G
Cuts at: 779 868 1253 1583 2650 3833 4174 5774 6008
Size: 89 385 330 1067 1183 341 1600 234
Cuts at: 6008 6295 6980 779
Size: 287 685 3047
Fragments arranged by size:
3047 1600 1183 1067 685 385 341 330
287 234 89
PvuI CG_AT'CG
Cuts at: 104 1225 104
Size: 1121 8127
PvuII CAG'CTG
Cuts at: 1097 1894 2231 2651 2955 4175 5475 5499 6060
Size: 797 337 420 304 1220 1300 24 561
Cuts at: 6060 6673 7033 1097
Size: 613 360 3312
Fragments arranged by size:
3312 1300 1220 797 613 561 420 360
337 304 24
RsrII CG'GwC_CG
Cuts at: 7443 7443
Size: 9248
SacI G_AGCT'C
Cuts at: 6262 6262
Size: 9248
SacII CC_GC'GG
Cuts at: 825 2774 825
Size: 1949 7299
SalI G'TCGA_C
Cuts at: 6285 6285
Size: 9248
SanDI GG'GwC_CC
Cuts at: 581 843 581
Size: 262 8986
SapI GCTCTTCn'nnn_
Cuts at: 697 4867 7271 7481 697
Size: 4170 2404 210 2464
Fragments arranged by size:
4170 2464 2404 210
SbfI CC_TGCA'GG
Cuts at: 1583 6008 6295 1583
Size: 4425 287 4536
Fragments arranged by size:
4536 4425 287
ScaI AGT'ACT
Cuts at: 8765 8765
Size: 9248
SexAI A'CCwGG_T
Cuts at: 341 422 341
Size: 81 9167
SfcI C'TryA_G
Cuts at: 33 756 775 864 1249 1380 1579 2280 2646
Size: 723 19 89 385 131 199 701 366
Cuts at: 2646 3829 4170 5770 6004 6291 6976 7969 8160
Size: 1183 341 1600 234 287 685 993 191
Cuts at: 8160 33
Size: 1121
Fragments arranged by size:
1600 1183 1121 993 723 701 685 385
366 341 287 234 199 191 131 89
19
SfiI GGCCn_nnn'nGGCC
Cuts at: 5129 5129
Size: 9248
SfoI GGC'GCC
Cuts at: 648 728 942 975 1074 1123 1169 2667 3114
Size: 80 214 33 99 49 46 1498 447
Cuts at: 3114 3802 4197 4221 4498 4647 4910 6051 6680
Size: 688 395 24 277 149 263 1141 629
Cuts at: 6680 6928 648
Size: 248 2968
Fragments arranged by size:
2968 1498 1141 688 629 447 395 277
263 248 214 149 99 80 49 46
33 24
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9248
SmaI CCC'GGG
Cuts at: 128 352 579 1237 2086 2452 2718 3769 4354
Size: 224 227 658 849 366 266 1051 585
Cuts at: 4354 5566 5640 6270 128
Size: 1212 74 630 3106
Fragments arranged by size:
3106 1212 1051 849 658 630 585 366
266 227 224 74
SmlI C'TyrA_G
Cuts at: 1590 3361 6403 7318 7810 8072 1590
Size: 1771 3042 915 492 262 2766
Fragments arranged by size:
3042 2766 1771 915 492 262
SnaBI TAC'GTA
Cuts at: 2337 5400 2337
Size: 3063 6185
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9248
SphI G_CATG'C
Cuts at: 1845 6301 6801 1845
Size: 4456 500 4292
Fragments arranged by size:
4456 4292 500
SrfI GCCC'GGGC
Cuts at: 2086 3769 2086
Size: 1683 7565
StuI AGG'CCT
Cuts at: 5057 6130 5057
Size: 1073 8175
StyI C'CwwG_G
Cuts at: 107 2499 2661 2909 3368 3500 3809 4782 4899
Size: 2392 162 248 459 132 309 973 117
Cuts at: 4899 5705 6125 6382 6736 107
Size: 806 420 257 354 2619
Fragments arranged by size:
2619 2392 973 806 459 420 354 309
257 248 162 132 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3270 5767 6831 6964 7423 7632 3270
Size: 2497 1064 133 459 209 4886
Fragments arranged by size:
4886 2497 1064 459 209 133
TatI w'GTAC_w
Cuts at: 2638 4337 8763 2638
Size: 1699 4426 3123
Fragments arranged by size:
4426 3123 1699
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 283 315 1907 1939 2197 2229 4289 4321 8765
Size: 32 1592 32 258 32 2060 32 4444
Cuts at: 8765 8797 283
Size: 32 734
Fragments arranged by size:
4444 2060 1592 734 258 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1189 1210 1360 1624 2014 6205 7045 1189
Size: 21 150 264 390 4191 840 3392
Fragments arranged by size:
4191 3392 840 390 264 150 21
XbaI T'CTAG_A
Cuts at: 6279 6279
Size: 9248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 430 1804 3337 5289 5817 6743 430
Size: 1374 1533 1952 528 926 2935
Fragments arranged by size:
2935 1952 1533 1374 926 528
XhoI C'TCGA_G
Cuts at: 1590 3361 1590
Size: 1771 7477
XmaI C'CCGG_G
Cuts at: 126 350 577 1235 2084 2450 2716 3767 4352
Size: 224 227 658 849 366 266 1051 585
Cuts at: 4352 5564 5638 6268 126
Size: 1212 74 630 3106
Fragments arranged by size:
3106 1212 1051 849 658 630 585 366
266 227 224 74
XmnI GAAnn'nnTTC
Cuts at: 1033 3661 1033
Size: 2628 6620
ZraI GAC'GTC
Cuts at: 3651 4069 4680 5535 3651
Size: 418 611 855 7364
Fragments arranged by size:
7364 855 611 418
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI AvrII BaeI BfrBI BmtI BsiWI
BsmBI BstZ17I ClaI EcoRV FspAI HindIII HpaI MfeI
NdeI NheI NsiI PacI PsiI PsrI SgrAI SspI
SwaI