Restriction map for
pF1KB9485
(Circular) (Six-base) MAPSORT of: pF1KB9485.seq Check: 5066 from: 1 to: 9302
pF1KB9485 9302 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4861 5424 4861
Size: 563 8739
AccI GT'mk_AC
Cuts at: 2902 4315 6340 2902
Size: 1413 2025 5864
Fragments arranged by size:
5864 2025 1413
Acc65I G'GTAC_C
Cuts at: 6318 6318
Size: 9302
AclI AA'CG_TT
Cuts at: 1386 9142 1386
Size: 7756 1546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 698 980 1364 1532 1835 2681 2972 3719
Size: 281 282 384 168 303 846 291 747
Cuts at: 3719 3845 4259 4313 4406 5075 5276 5723 5732
Size: 126 414 54 93 669 201 447 9
Cuts at: 5732 7127 7559 7912 8574 417
Size: 1395 432 353 662 1145
Fragments arranged by size:
1395 1145 846 747 669 662 447 432
414 384 353 303 291 282 281 201
168 126 93 54 9
AflIII A'CryG_T
Cuts at: 4195 6654 8454 8735 4195
Size: 2459 1800 281 4762
Fragments arranged by size:
4762 2459 1800 281
AgeI A'CCGG_T
Cuts at: 1578 1578
Size: 9302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1181 4706 1181
Size: 3525 5777
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 327 359 327
Size: 32 9270
AlwNI CAG_nnn'CTG
Cuts at: 1198 1783 2815 3916 4336 5001 6076 6607 8045
Size: 585 1032 1101 420 665 1075 531 1438
Cuts at: 8045 1198
Size: 2455
Fragments arranged by size:
2455 1438 1101 1075 1032 665 585 531
420
ApaLI G'TGCA_C
Cuts at: 2912 8140 2912
Size: 5228 4074
ApoI r'AATT_y
Cuts at: 740 871 1047 1845 2185 2307 2349 2422 2510
Size: 131 176 798 340 122 42 73 88
Cuts at: 2510 3642 3729 4131 4725 5200 5653 6249 6306
Size: 1132 87 402 594 475 453 596 57
Cuts at: 6306 8655 8911 740
Size: 2349 256 1131
Fragments arranged by size:
2349 1132 1131 798 596 594 475 453
402 340 256 176 131 122 88 87
73 57 42
AseI AT'TA_AT
Cuts at: 20 551 4399 5573 20
Size: 531 3848 1174 3749
Fragments arranged by size:
3848 3749 1174 531
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9302
AvaI C'yCGr_G
Cuts at: 6322 6322
Size: 9302
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 360 393 2384 2417 4883 4916 360
Size: 33 1991 33 2466 33 4746
Fragments arranged by size:
4746 2466 1991 33 33 33
BamHI G'GATC_C
Cuts at: 6327 6327
Size: 9302
BanI G'GyrC_C
Cuts at: 211 1575 6318 6732 6980 7015 211
Size: 1364 4743 414 248 35 2498
Fragments arranged by size:
4743 2498 1364 414 248 35
BanII G_rGCy'C
Cuts at: 1110 2007 3194 6288 6316 7346 1110
Size: 897 1187 3094 28 1030 3066
Fragments arranged by size:
3094 3066 1187 1030 897 28
BbeI G_GCGC'C
Cuts at: 6736 6984 6736
Size: 248 9054
BbsI GAAGACnn'nnnn_
Cuts at: 1326 2342 2712 2942 3978 4822 5444 1326
Size: 1016 370 230 1036 844 622 5184
Fragments arranged by size:
5184 1036 1016 844 622 370 230
BbvCI CC'TCA_GC
Cuts at: 1019 2858 1019
Size: 1839 7463
BciVI GTATCCnnnnn_n'
Cuts at: 4524 7200 8256 9302 4524
Size: 2676 1056 1046 4524
Fragments arranged by size:
4524 2676 1056 1046
BclI T'GATC_A
Cuts at: 147 5529 6822 147
Size: 5382 1293 2627
Fragments arranged by size:
5382 2627 1293
BfrBI ATG'CAT
Cuts at: 574 4498 574
Size: 3924 5378
BglI GCCn_nnn'nGGC
Cuts at: 4087 5383 4087
Size: 1296 8006
BglII A'GATC_T
Cuts at: 780 802 1984 2244 5235 5515 6162 6817 8854
Size: 22 1182 260 2991 280 647 655 2037
Cuts at: 8854 780
Size: 1228
Fragments arranged by size:
2991 2037 1228 1182 655 647 280 260
22
BlpI GC'TnA_GC
Cuts at: 6414 6414
Size: 9302
Bme1580I G_kGCm'C
Cuts at: 216 2916 6927 7020 8144 216
Size: 2700 4011 93 1124 1374
Fragments arranged by size:
4011 2700 1374 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2901 3542 3695 4346 6514 6595 6930 9074 2901
Size: 641 153 651 2168 81 335 2144 3129
Fragments arranged by size:
3129 2168 2144 651 641 335 153 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7669 7669
Size: 9302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 1283 1678 2417 2792 3524 5378 5441 5674
Size: 1097 395 739 375 732 1854 63 233
Cuts at: 5674 6029 186
Size: 355 3459
Fragments arranged by size:
3459 1854 1097 739 732 395 375 355
233 63
Bpu10I CC'TnA_GC
Cuts at: 1019 2858 3720 5996 1019
Size: 1839 862 2276 4325
Fragments arranged by size:
4325 2276 1839 862
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 3356 4497 6478 7357 8071 8369 2264
Size: 1092 1141 1981 879 714 298 3197
Fragments arranged by size:
3197 1981 1141 1092 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2623 9006 2623
Size: 6383 2919
BsaAI yAC'GTr
Cuts at: 3201 6655 3201
Size: 3454 5848
BsaBI GATnn'nnATC
Cuts at: 6 443 1493 2168 6816 8853 6
Size: 437 1050 675 4648 2037 455
Fragments arranged by size:
4648 2037 1050 675 455 437
BsaHI Gr'CG_yC
Cuts at: 1217 3975 6733 6981 7683 9179 1217
Size: 2758 2758 248 702 1496 1340
Fragments arranged by size:
2758 2758 1496 1340 702 248
BsaWI w'CCGG_w
Cuts at: 303 1578 6491 7012 7690 8101 8248 8522 303
Size: 1275 4913 521 678 411 147 274 1083
Fragments arranged by size:
4913 1275 1083 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 327 357 1536 1566 2772 2790 2802 2820 4538
Size: 30 1179 30 1206 18 12 18 1718
Cuts at: 4538 4568 6027 6057 327
Size: 30 1459 30 3572
Fragments arranged by size:
3572 1718 1459 1206 1179 30 30 30
30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 134 919 922 2104 2107 2110 2128 2740 2793
Size: 785 3 1182 3 3 18 612 53
Cuts at: 2793 2846 2992 3359 3844 5064 134
Size: 53 146 367 485 1220 4372
Fragments arranged by size:
4372 1220 1182 785 612 485 367 146
53 53 18 3 3 3
BseYI C'CCAG_C
Cuts at: 2325 3352 5120 6727 7485 8150 8558 2325
Size: 1027 1768 1607 758 665 408 3069
Fragments arranged by size:
3069 1768 1607 1027 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1112 4058 4907 1003
Size: 109 2946 849 5398
Fragments arranged by size:
5398 2946 849 109
BsiEI CG_ry'CG
Cuts at: 104 6844 6890 8120 104
Size: 6740 46 1230 1286
Fragments arranged by size:
6740 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 964 1110 2916 3194 5057 6316 7094 7284 8144
Size: 146 1806 278 1863 1259 778 190 860
Cuts at: 8144 964
Size: 2122
Fragments arranged by size:
2122 1863 1806 1259 860 778 278 190
146
Bsp1286I G_dGCh'C
Cuts at: 216 964 1110 2007 2916 3194 5057 6288 6316
Size: 748 146 897 909 278 1863 1231 28
Cuts at: 6316 6927 7020 7094 7284 7346 8144 216
Size: 611 93 74 190 62 798 1374
Fragments arranged by size:
1863 1374 1231 909 897 798 748 611
278 190 146 93 74 62 28
BspEI T'CCGG_A
Cuts at: 303 303
Size: 9302
BspHI T'CATG_A
Cuts at: 4278 7734 4278
Size: 3456 5846
BspMI ACCTGCnnnn'nnnn_
Cuts at: 708 1192 4861 4879 5424 6352 6566 6868 7249
Size: 484 3669 18 545 928 214 302 381
Cuts at: 7249 708
Size: 2761
Fragments arranged by size:
3669 2761 928 545 484 381 302 214
18
BsrBI CCG'CTC
Cuts at: 50 7594 7648 9132 9297 50
Size: 7544 54 1484 165 55
Fragments arranged by size:
7544 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 710 2518 3363 4498 7214 710
Size: 1808 845 1135 2716 2798
Fragments arranged by size:
2798 2716 1808 1135 845
BsrFI r'CCGG_y
Cuts at: 1578 5006 7300 7481 1578
Size: 3428 2294 181 3399
Fragments arranged by size:
3428 3399 2294 181
BssSI C'ACGA_G
Cuts at: 2626 7573 8281 2626
Size: 4947 708 3647
Fragments arranged by size:
4947 3647 708
BstAPI GCAn_nnn'nTGC
Cuts at: 487 1198 5203 6679 487
Size: 711 4005 1476 3110
Fragments arranged by size:
4005 3110 1476 711
BstBI TT'CG_AA
Cuts at: 1713 1869 7663 1713
Size: 156 5794 3352
Fragments arranged by size:
5794 3352 156
BstEII G'GTnAC_C
Cuts at: 2941 2941
Size: 9302
BstXI CCAn_nnnn'nTGG
Cuts at: 6208 6208
Size: 9302
BstYI r'GATC_y
Cuts at: 1 780 802 940 1014 1984 2244 2836 4029
Size: 779 22 138 74 970 260 592 1193
Cuts at: 4029 5235 5515 6162 6327 6794 6817 7152 7398
Size: 1206 280 647 165 467 23 335 246
Cuts at: 7398 7704 7716 7802 8854 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1206 1193 1052 970 779 647 592 467
449 335 306 280 260 246 165 138
86 74 23 22 12
BtgI C'CryG_G
Cuts at: 107 1004 3853 107
Size: 897 2849 5556
Fragments arranged by size:
5556 2849 897
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 950 3515 7252 7433 7596 950
Size: 2565 3737 181 163 2656
Fragments arranged by size:
3737 2656 2565 181 163
BtsI GCAGTG_nn'
Cuts at: 1175 6545 1175
Size: 5370 3932
DraI TTT'AAA
Cuts at: 5754 5873 6301 5754
Size: 119 428 8755
Fragments arranged by size:
8755 428 119
DraIII CAC_nnn'GTG
Cuts at: 1927 2077 3703 1927
Size: 150 1626 7526
Fragments arranged by size:
7526 1626 150
DrdI GACnn_nn'nnGTC
Cuts at: 7008 8352 7008
Size: 1344 7958
EaeI y'GGCC_r
Cuts at: 110 1007 6887 7061 7452 7479 8824 110
Size: 897 5880 174 391 27 1345 588
Fragments arranged by size:
5880 1345 897 588 391 174 27
EagI C'GGCC_G
Cuts at: 6887 6887
Size: 9302
EarI CTCTTCn'nnn_
Cuts at: 1031 1811 2105 2969 2981 3200 3329 3598 3821
Size: 780 294 864 12 219 129 269 223
Cuts at: 3821 4120 4235 4447 7325 7535 1031
Size: 299 115 212 2878 210 2798
Fragments arranged by size:
2878 2798 864 780 299 294 269 223
219 212 210 129 115 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7510 8252 8398 9112 7510
Size: 742 146 714 7700
Fragments arranged by size:
7700 742 714 146
EcoICRI GAG'CTC
Cuts at: 1108 3192 6314 1108
Size: 2084 3122 4096
Fragments arranged by size:
4096 3122 2084
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 417 698 980 1283 1364 1532 1678 1835
Size: 231 281 282 303 81 168 146 157
Cuts at: 1835 2417 2681 2792 2972 3524 3719 3845 4259
Size: 582 264 111 180 552 195 126 414
Cuts at: 4259 4313 4406 5075 5276 5378 5441 5674 5723
Size: 54 93 669 201 102 63 233 49
Cuts at: 5723 5732 6029 7127 7559 7912 8574 186
Size: 9 297 1098 432 353 662 914
Fragments arranged by size:
1098 914 669 662 582 552 432 414
353 303 297 282 281 264 233 231
201 195 180 168 157 146 126 111
102 93 81 63 54 49 9
EcoO109I rG'GnC_Cy
Cuts at: 124 3334 5665 5736 6441 124
Size: 3210 2331 71 705 2985
Fragments arranged by size:
3210 2985 2331 705 71
EcoRI G'AATT_C
Cuts at: 3729 4131 6306 3729
Size: 402 2175 6725
Fragments arranged by size:
6725 2175 402
EcoRV GAT'ATC
Cuts at: 5252 5252
Size: 9302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1426 3190 5257 7321 1426
Size: 1764 2067 2064 3407
Fragments arranged by size:
3407 2067 2064 1764
FspI TGC'GCA
Cuts at: 7083 7083
Size: 9302
HaeII r_GCGC'y
Cuts at: 822 6736 6984 8214 8985 822
Size: 5914 248 1230 771 1139
Fragments arranged by size:
5914 1230 1139 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 949 970 981 1002 1518 1550 2677 2709 2772
Size: 21 11 21 516 32 1127 32 63
Cuts at: 2772 2804 3976 4008 4498 4530 5395 5427 6025
Size: 32 1172 32 490 32 865 32 598
Cuts at: 6025 6057 7144 7174 7176 7206 949
Size: 32 1087 30 2 30 3045
Fragments arranged by size:
3045 1172 1127 1087 865 598 516 490
63 32 32 32 32 32 32 32
30 30 21 21 11 2
HincII GTy'rAC
Cuts at: 6341 6341
Size: 9302
HindIII A'AGCT_T
Cuts at: 4002 4361 5287 4002
Size: 359 926 8017
Fragments arranged by size:
8017 926 359
KasI G'GCGC_C
Cuts at: 6732 6980 6732
Size: 248 9054
KpnI G_GTAC'C
Cuts at: 6322 6322
Size: 9302
MfeI C'AATT_G
Cuts at: 2467 3090 2467
Size: 623 8679
MluI A'CGCG_T
Cuts at: 8735 8735
Size: 9302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 303 3598 4057 8061 8245 303
Size: 3295 459 4004 184 1360
Fragments arranged by size:
4004 3295 1360 459 184
MscI TGG'CCA
Cuts at: 112 7063 112
Size: 6951 2351
MslI CAynn'nnrTG
Cuts at: 979 1751 1916 5060 7291 7418 979
Size: 772 165 3144 2231 127 2863
Fragments arranged by size:
3144 2863 2231 772 165 127
MspA1I CmG'CkG
Cuts at: 423 487 934 1303 2547 2554 2596 4143 5001
Size: 64 447 369 1244 7 42 1547 858
Cuts at: 5001 6413 6727 7087 7871 8116 423
Size: 1412 314 360 784 245 1609
Fragments arranged by size:
1609 1547 1412 1244 858 784 447 369
360 314 245 64 42 7
NaeI GCC'GGC
Cuts at: 7483 7483
Size: 9302
NarI GG'CG_CC
Cuts at: 6733 6981 6733
Size: 248 9054
NcoI C'CATG_G
Cuts at: 107 3853 107
Size: 3746 5556
NgoMIV G'CCGG_C
Cuts at: 7481 7481
Size: 9302
NruI TCG'CGA
Cuts at: 746 746
Size: 9302
NsiI A_TGCA'T
Cuts at: 576 4500 576
Size: 3924 5378
NspI r_CATG'y
Cuts at: 2534 3159 4199 6355 6855 8458 2534
Size: 625 1040 2156 500 1603 3378
Fragments arranged by size:
3378 2156 1603 1040 625 500
PciI A'CATG_T
Cuts at: 4195 8454 4195
Size: 4259 5043
PflMI CCAn_nnn'nTGG
Cuts at: 322 322
Size: 9302
PmeI GTTT'AAAC
Cuts at: 6301 6301
Size: 9302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 327 359 1727 1759 7748 7780 327
Size: 32 1368 32 5989 32 1849
Fragments arranged by size:
5989 1849 1368 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 124 3334 5736 124
Size: 3210 2402 3690
Fragments arranged by size:
3690 3210 2402
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5723 5755 5723
Size: 32 9270
PstI C_TGCA'G
Cuts at: 1203 4457 4858 6349 7034 1203
Size: 3254 401 1491 685 3471
Fragments arranged by size:
3471 3254 1491 685 401
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9302
PvuII CAG'CTG
Cuts at: 487 934 1303 2547 2554 2596 4143 5001 6727
Size: 447 369 1244 7 42 1547 858 1726
Cuts at: 6727 7087 487
Size: 360 2702
Fragments arranged by size:
2702 1726 1547 1244 858 447 369 360
42 7
RsrII CG'GwC_CG
Cuts at: 7497 7497
Size: 9302
SacI G_AGCT'C
Cuts at: 1110 3194 6316 1110
Size: 2084 3122 4096
Fragments arranged by size:
4096 3122 2084
SalI G'TCGA_C
Cuts at: 6339 6339
Size: 9302
SapI GCTCTTCn'nnn_
Cuts at: 2981 4235 7325 7535 2981
Size: 1254 3090 210 4748
Fragments arranged by size:
4748 3090 1254 210
SbfI CC_TGCA'GG
Cuts at: 4858 6349 4858
Size: 1491 7811
ScaI AGT'ACT
Cuts at: 1545 8819 1545
Size: 7274 2028
SexAI A'CCwGG_T
Cuts at: 3633 3633
Size: 9302
SfcI C'TryA_G
Cuts at: 33 923 950 1111 1199 1432 1565 2440 3917
Size: 890 27 161 88 233 133 875 1477
Cuts at: 3917 4316 4453 4854 4895 6345 7030 7998 8189
Size: 399 137 401 41 1450 685 968 191
Cuts at: 8189 33
Size: 1146
Fragments arranged by size:
1477 1450 1146 968 890 875 685 401
399 233 191 161 137 133 88 41
27
SfoI GGC'GCC
Cuts at: 6734 6982 6734
Size: 248 9054
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9302
SmaI CCC'GGG
Cuts at: 6324 6324
Size: 9302
SmlI C'TyrA_G
Cuts at: 2279 3371 4476 6457 7372 8086 8348 2279
Size: 1092 1105 1981 915 714 262 3233
Fragments arranged by size:
3233 1981 1105 1092 915 714 262
SnaBI TAC'GTA
Cuts at: 3201 3201
Size: 9302
SpeI A'CTAG_T
Cuts at: 67 5800 67
Size: 5733 3569
SphI G_CATG'C
Cuts at: 2534 3159 6355 6855 2534
Size: 625 3196 500 4981
Fragments arranged by size:
4981 3196 625 500
StyI C'CwwG_G
Cuts at: 107 471 3805 3853 6436 6790 107
Size: 364 3334 48 2583 354 2619
Fragments arranged by size:
3334 2619 2583 364 354 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3171 3714 6247 6885 7018 7477 7686 3171
Size: 543 2533 638 133 459 209 4787
Fragments arranged by size:
4787 2533 638 543 459 209 133
TatI w'GTAC_w
Cuts at: 1543 2635 2851 3375 8817 1543
Size: 1092 216 524 5442 2028
Fragments arranged by size:
5442 2028 1092 524 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 338 370 3896 3928 5549 5581 8819 8851 338
Size: 32 3526 32 1621 32 3238 32 789
Fragments arranged by size:
3526 3238 1621 789 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7099 7099
Size: 9302
XbaI T'CTAG_A
Cuts at: 6333 6333
Size: 9302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6797 6797
Size: 9302
XmaI C'CCGG_G
Cuts at: 6322 6322
Size: 9302
XmnI GAAnn'nnTTC
Cuts at: 465 1545 1742 3173 5036 5237 465
Size: 1080 197 1431 1863 201 4530
Fragments arranged by size:
4530 1863 1431 1080 201 197
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NsiI NspI PciI PflMI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BcgI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HpaI
NdeI NheI NotI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI