(Circular) (Six-base) MAPSORT of: pF1KB9485.seq Check: 5066 from: 1 to: 9302 pF1KB9485 9302 bp With 182 enzymes: SgfI * March 2, 2010 14:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4861 5424 4861 Size: 563 8739 AccI GT'mk_AC Cuts at: 2902 4315 6340 2902 Size: 1413 2025 5864 Fragments arranged by size: 5864 2025 1413 Acc65I G'GTAC_C Cuts at: 6318 6318 Size: 9302 AclI AA'CG_TT Cuts at: 1386 9142 1386 Size: 7756 1546 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 417 698 980 1364 1532 1835 2681 2972 3719 Size: 281 282 384 168 303 846 291 747 Cuts at: 3719 3845 4259 4313 4406 5075 5276 5723 5732 Size: 126 414 54 93 669 201 447 9 Cuts at: 5732 7127 7559 7912 8574 417 Size: 1395 432 353 662 1145 Fragments arranged by size: 1395 1145 846 747 669 662 447 432 414 384 353 303 291 282 281 201 168 126 93 54 9 AflIII A'CryG_T Cuts at: 4195 6654 8454 8735 4195 Size: 2459 1800 281 4762 Fragments arranged by size: 4762 2459 1800 281 AgeI A'CCGG_T Cuts at: 1578 1578 Size: 9302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1181 4706 1181 Size: 3525 5777 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 327 359 327 Size: 32 9270 AlwNI CAG_nnn'CTG Cuts at: 1198 1783 2815 3916 4336 5001 6076 6607 8045 Size: 585 1032 1101 420 665 1075 531 1438 Cuts at: 8045 1198 Size: 2455 Fragments arranged by size: 2455 1438 1101 1075 1032 665 585 531 420 ApaLI G'TGCA_C Cuts at: 2912 8140 2912 Size: 5228 4074 ApoI r'AATT_y Cuts at: 740 871 1047 1845 2185 2307 2349 2422 2510 Size: 131 176 798 340 122 42 73 88 Cuts at: 2510 3642 3729 4131 4725 5200 5653 6249 6306 Size: 1132 87 402 594 475 453 596 57 Cuts at: 6306 8655 8911 740 Size: 2349 256 1131 Fragments arranged by size: 2349 1132 1131 798 596 594 475 453 402 340 256 176 131 122 88 87 73 57 42 AseI AT'TA_AT Cuts at: 20 551 4399 5573 20 Size: 531 3848 1174 3749 Fragments arranged by size: 3848 3749 1174 531 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 9302 AvaI C'yCGr_G Cuts at: 6322 6322 Size: 9302 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 360 393 2384 2417 4883 4916 360 Size: 33 1991 33 2466 33 4746 Fragments arranged by size: 4746 2466 1991 33 33 33 BamHI G'GATC_C Cuts at: 6327 6327 Size: 9302 BanI G'GyrC_C Cuts at: 211 1575 6318 6732 6980 7015 211 Size: 1364 4743 414 248 35 2498 Fragments arranged by size: 4743 2498 1364 414 248 35 BanII G_rGCy'C Cuts at: 1110 2007 3194 6288 6316 7346 1110 Size: 897 1187 3094 28 1030 3066 Fragments arranged by size: 3094 3066 1187 1030 897 28 BbeI G_GCGC'C Cuts at: 6736 6984 6736 Size: 248 9054 BbsI GAAGACnn'nnnn_ Cuts at: 1326 2342 2712 2942 3978 4822 5444 1326 Size: 1016 370 230 1036 844 622 5184 Fragments arranged by size: 5184 1036 1016 844 622 370 230 BbvCI CC'TCA_GC Cuts at: 1019 2858 1019 Size: 1839 7463 BciVI GTATCCnnnnn_n' Cuts at: 4524 7200 8256 9302 4524 Size: 2676 1056 1046 4524 Fragments arranged by size: 4524 2676 1056 1046 BclI T'GATC_A Cuts at: 147 5529 6822 147 Size: 5382 1293 2627 Fragments arranged by size: 5382 2627 1293 BfrBI ATG'CAT Cuts at: 574 4498 574 Size: 3924 5378 BglI GCCn_nnn'nGGC Cuts at: 4087 5383 4087 Size: 1296 8006 BglII A'GATC_T Cuts at: 780 802 1984 2244 5235 5515 6162 6817 8854 Size: 22 1182 260 2991 280 647 655 2037 Cuts at: 8854 780 Size: 1228 Fragments arranged by size: 2991 2037 1228 1182 655 647 280 260 22 BlpI GC'TnA_GC Cuts at: 6414 6414 Size: 9302 Bme1580I G_kGCm'C Cuts at: 216 2916 6927 7020 8144 216 Size: 2700 4011 93 1124 1374 Fragments arranged by size: 4011 2700 1374 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 2901 3542 3695 4346 6514 6595 6930 9074 2901 Size: 641 153 651 2168 81 335 2144 3129 Fragments arranged by size: 3129 2168 2144 651 641 335 153 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7669 7669 Size: 9302 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 186 1283 1678 2417 2792 3524 5378 5441 5674 Size: 1097 395 739 375 732 1854 63 233 Cuts at: 5674 6029 186 Size: 355 3459 Fragments arranged by size: 3459 1854 1097 739 732 395 375 355 233 63 Bpu10I CC'TnA_GC Cuts at: 1019 2858 3720 5996 1019 Size: 1839 862 2276 4325 Fragments arranged by size: 4325 2276 1839 862 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2264 3356 4497 6478 7357 8071 8369 2264 Size: 1092 1141 1981 879 714 298 3197 Fragments arranged by size: 3197 1981 1141 1092 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 2623 9006 2623 Size: 6383 2919 BsaAI yAC'GTr Cuts at: 3201 6655 3201 Size: 3454 5848 BsaBI GATnn'nnATC Cuts at: 6 443 1493 2168 6816 8853 6 Size: 437 1050 675 4648 2037 455 Fragments arranged by size: 4648 2037 1050 675 455 437 BsaHI Gr'CG_yC Cuts at: 1217 3975 6733 6981 7683 9179 1217 Size: 2758 2758 248 702 1496 1340 Fragments arranged by size: 2758 2758 1496 1340 702 248 BsaWI w'CCGG_w Cuts at: 303 1578 6491 7012 7690 8101 8248 8522 303 Size: 1275 4913 521 678 411 147 274 1083 Fragments arranged by size: 4913 1275 1083 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 327 357 1536 1566 2772 2790 2802 2820 4538 Size: 30 1179 30 1206 18 12 18 1718 Cuts at: 4538 4568 6027 6057 327 Size: 30 1459 30 3572 Fragments arranged by size: 3572 1718 1459 1206 1179 30 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 134 919 922 2104 2107 2110 2128 2740 2793 Size: 785 3 1182 3 3 18 612 53 Cuts at: 2793 2846 2992 3359 3844 5064 134 Size: 53 146 367 485 1220 4372 Fragments arranged by size: 4372 1220 1182 785 612 485 367 146 53 53 18 3 3 3 BseYI C'CCAG_C Cuts at: 2325 3352 5120 6727 7485 8150 8558 2325 Size: 1027 1768 1607 758 665 408 3069 Fragments arranged by size: 3069 1768 1607 1027 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1003 1112 4058 4907 1003 Size: 109 2946 849 5398 Fragments arranged by size: 5398 2946 849 109 BsiEI CG_ry'CG Cuts at: 104 6844 6890 8120 104 Size: 6740 46 1230 1286 Fragments arranged by size: 6740 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 964 1110 2916 3194 5057 6316 7094 7284 8144 Size: 146 1806 278 1863 1259 778 190 860 Cuts at: 8144 964 Size: 2122 Fragments arranged by size: 2122 1863 1806 1259 860 778 278 190 146 Bsp1286I G_dGCh'C Cuts at: 216 964 1110 2007 2916 3194 5057 6288 6316 Size: 748 146 897 909 278 1863 1231 28 Cuts at: 6316 6927 7020 7094 7284 7346 8144 216 Size: 611 93 74 190 62 798 1374 Fragments arranged by size: 1863 1374 1231 909 897 798 748 611 278 190 146 93 74 62 28 BspEI T'CCGG_A Cuts at: 303 303 Size: 9302 BspHI T'CATG_A Cuts at: 4278 7734 4278 Size: 3456 5846 BspMI ACCTGCnnnn'nnnn_ Cuts at: 708 1192 4861 4879 5424 6352 6566 6868 7249 Size: 484 3669 18 545 928 214 302 381 Cuts at: 7249 708 Size: 2761 Fragments arranged by size: 3669 2761 928 545 484 381 302 214 18 BsrBI CCG'CTC Cuts at: 50 7594 7648 9132 9297 50 Size: 7544 54 1484 165 55 Fragments arranged by size: 7544 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 710 2518 3363 4498 7214 710 Size: 1808 845 1135 2716 2798 Fragments arranged by size: 2798 2716 1808 1135 845 BsrFI r'CCGG_y Cuts at: 1578 5006 7300 7481 1578 Size: 3428 2294 181 3399 Fragments arranged by size: 3428 3399 2294 181 BssSI C'ACGA_G Cuts at: 2626 7573 8281 2626 Size: 4947 708 3647 Fragments arranged by size: 4947 3647 708 BstAPI GCAn_nnn'nTGC Cuts at: 487 1198 5203 6679 487 Size: 711 4005 1476 3110 Fragments arranged by size: 4005 3110 1476 711 BstBI TT'CG_AA Cuts at: 1713 1869 7663 1713 Size: 156 5794 3352 Fragments arranged by size: 5794 3352 156 BstEII G'GTnAC_C Cuts at: 2941 2941 Size: 9302 BstXI CCAn_nnnn'nTGG Cuts at: 6208 6208 Size: 9302 BstYI r'GATC_y Cuts at: 1 780 802 940 1014 1984 2244 2836 4029 Size: 779 22 138 74 970 260 592 1193 Cuts at: 4029 5235 5515 6162 6327 6794 6817 7152 7398 Size: 1206 280 647 165 467 23 335 246 Cuts at: 7398 7704 7716 7802 8854 1 Size: 306 12 86 1052 449 Fragments arranged by size: 1206 1193 1052 970 779 647 592 467 449 335 306 280 260 246 165 138 86 74 23 22 12 BtgI C'CryG_G Cuts at: 107 1004 3853 107 Size: 897 2849 5556 Fragments arranged by size: 5556 2849 897 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 950 3515 7252 7433 7596 950 Size: 2565 3737 181 163 2656 Fragments arranged by size: 3737 2656 2565 181 163 BtsI GCAGTG_nn' Cuts at: 1175 6545 1175 Size: 5370 3932 DraI TTT'AAA Cuts at: 5754 5873 6301 5754 Size: 119 428 8755 Fragments arranged by size: 8755 428 119 DraIII CAC_nnn'GTG Cuts at: 1927 2077 3703 1927 Size: 150 1626 7526 Fragments arranged by size: 7526 1626 150 DrdI GACnn_nn'nnGTC Cuts at: 7008 8352 7008 Size: 1344 7958 EaeI y'GGCC_r Cuts at: 110 1007 6887 7061 7452 7479 8824 110 Size: 897 5880 174 391 27 1345 588 Fragments arranged by size: 5880 1345 897 588 391 174 27 EagI C'GGCC_G Cuts at: 6887 6887 Size: 9302 EarI CTCTTCn'nnn_ Cuts at: 1031 1811 2105 2969 2981 3200 3329 3598 3821 Size: 780 294 864 12 219 129 269 223 Cuts at: 3821 4120 4235 4447 7325 7535 1031 Size: 299 115 212 2878 210 2798 Fragments arranged by size: 2878 2798 864 780 299 294 269 223 219 212 210 129 115 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 7510 8252 8398 9112 7510 Size: 742 146 714 7700 Fragments arranged by size: 7700 742 714 146 EcoICRI GAG'CTC Cuts at: 1108 3192 6314 1108 Size: 2084 3122 4096 Fragments arranged by size: 4096 3122 2084 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 186 417 698 980 1283 1364 1532 1678 1835 Size: 231 281 282 303 81 168 146 157 Cuts at: 1835 2417 2681 2792 2972 3524 3719 3845 4259 Size: 582 264 111 180 552 195 126 414 Cuts at: 4259 4313 4406 5075 5276 5378 5441 5674 5723 Size: 54 93 669 201 102 63 233 49 Cuts at: 5723 5732 6029 7127 7559 7912 8574 186 Size: 9 297 1098 432 353 662 914 Fragments arranged by size: 1098 914 669 662 582 552 432 414 353 303 297 282 281 264 233 231 201 195 180 168 157 146 126 111 102 93 81 63 54 49 9 EcoO109I rG'GnC_Cy Cuts at: 124 3334 5665 5736 6441 124 Size: 3210 2331 71 705 2985 Fragments arranged by size: 3210 2985 2331 705 71 EcoRI G'AATT_C Cuts at: 3729 4131 6306 3729 Size: 402 2175 6725 Fragments arranged by size: 6725 2175 402 EcoRV GAT'ATC Cuts at: 5252 5252 Size: 9302 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1426 3190 5257 7321 1426 Size: 1764 2067 2064 3407 Fragments arranged by size: 3407 2067 2064 1764 FspI TGC'GCA Cuts at: 7083 7083 Size: 9302 HaeII r_GCGC'y Cuts at: 822 6736 6984 8214 8985 822 Size: 5914 248 1230 771 1139 Fragments arranged by size: 5914 1230 1139 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 949 970 981 1002 1518 1550 2677 2709 2772 Size: 21 11 21 516 32 1127 32 63 Cuts at: 2772 2804 3976 4008 4498 4530 5395 5427 6025 Size: 32 1172 32 490 32 865 32 598 Cuts at: 6025 6057 7144 7174 7176 7206 949 Size: 32 1087 30 2 30 3045 Fragments arranged by size: 3045 1172 1127 1087 865 598 516 490 63 32 32 32 32 32 32 32 30 30 21 21 11 2 HincII GTy'rAC Cuts at: 6341 6341 Size: 9302 HindIII A'AGCT_T Cuts at: 4002 4361 5287 4002 Size: 359 926 8017 Fragments arranged by size: 8017 926 359 KasI G'GCGC_C Cuts at: 6732 6980 6732 Size: 248 9054 KpnI G_GTAC'C Cuts at: 6322 6322 Size: 9302 MfeI C'AATT_G Cuts at: 2467 3090 2467 Size: 623 8679 MluI A'CGCG_T Cuts at: 8735 8735 Size: 9302 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 303 3598 4057 8061 8245 303 Size: 3295 459 4004 184 1360 Fragments arranged by size: 4004 3295 1360 459 184 MscI TGG'CCA Cuts at: 112 7063 112 Size: 6951 2351 MslI CAynn'nnrTG Cuts at: 979 1751 1916 5060 7291 7418 979 Size: 772 165 3144 2231 127 2863 Fragments arranged by size: 3144 2863 2231 772 165 127 MspA1I CmG'CkG Cuts at: 423 487 934 1303 2547 2554 2596 4143 5001 Size: 64 447 369 1244 7 42 1547 858 Cuts at: 5001 6413 6727 7087 7871 8116 423 Size: 1412 314 360 784 245 1609 Fragments arranged by size: 1609 1547 1412 1244 858 784 447 369 360 314 245 64 42 7 NaeI GCC'GGC Cuts at: 7483 7483 Size: 9302 NarI GG'CG_CC Cuts at: 6733 6981 6733 Size: 248 9054 NcoI C'CATG_G Cuts at: 107 3853 107 Size: 3746 5556 NgoMIV G'CCGG_C Cuts at: 7481 7481 Size: 9302 NruI TCG'CGA Cuts at: 746 746 Size: 9302 NsiI A_TGCA'T Cuts at: 576 4500 576 Size: 3924 5378 NspI r_CATG'y Cuts at: 2534 3159 4199 6355 6855 8458 2534 Size: 625 1040 2156 500 1603 3378 Fragments arranged by size: 3378 2156 1603 1040 625 500 PciI A'CATG_T Cuts at: 4195 8454 4195 Size: 4259 5043 PflMI CCAn_nnn'nTGG Cuts at: 322 322 Size: 9302 PmeI GTTT'AAAC Cuts at: 6301 6301 Size: 9302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 327 359 1727 1759 7748 7780 327 Size: 32 1368 32 5989 32 1849 Fragments arranged by size: 5989 1849 1368 32 32 32 PpuMI rG'GwC_Cy Cuts at: 124 3334 5736 124 Size: 3210 2402 3690 Fragments arranged by size: 3690 3210 2402 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5723 5755 5723 Size: 32 9270 PstI C_TGCA'G Cuts at: 1203 4457 4858 6349 7034 1203 Size: 3254 401 1491 685 3471 Fragments arranged by size: 3471 3254 1491 685 401 PvuI CG_AT'CG Cuts at: 104 104 Size: 9302 PvuII CAG'CTG Cuts at: 487 934 1303 2547 2554 2596 4143 5001 6727 Size: 447 369 1244 7 42 1547 858 1726 Cuts at: 6727 7087 487 Size: 360 2702 Fragments arranged by size: 2702 1726 1547 1244 858 447 369 360 42 7 RsrII CG'GwC_CG Cuts at: 7497 7497 Size: 9302 SacI G_AGCT'C Cuts at: 1110 3194 6316 1110 Size: 2084 3122 4096 Fragments arranged by size: 4096 3122 2084 SalI G'TCGA_C Cuts at: 6339 6339 Size: 9302 SapI GCTCTTCn'nnn_ Cuts at: 2981 4235 7325 7535 2981 Size: 1254 3090 210 4748 Fragments arranged by size: 4748 3090 1254 210 SbfI CC_TGCA'GG Cuts at: 4858 6349 4858 Size: 1491 7811 ScaI AGT'ACT Cuts at: 1545 8819 1545 Size: 7274 2028 SexAI A'CCwGG_T Cuts at: 3633 3633 Size: 9302 SfcI C'TryA_G Cuts at: 33 923 950 1111 1199 1432 1565 2440 3917 Size: 890 27 161 88 233 133 875 1477 Cuts at: 3917 4316 4453 4854 4895 6345 7030 7998 8189 Size: 399 137 401 41 1450 685 968 191 Cuts at: 8189 33 Size: 1146 Fragments arranged by size: 1477 1450 1146 968 890 875 685 401 399 233 191 161 137 133 88 41 27 SfoI GGC'GCC Cuts at: 6734 6982 6734 Size: 248 9054 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 9302 SmaI CCC'GGG Cuts at: 6324 6324 Size: 9302 SmlI C'TyrA_G Cuts at: 2279 3371 4476 6457 7372 8086 8348 2279 Size: 1092 1105 1981 915 714 262 3233 Fragments arranged by size: 3233 1981 1105 1092 915 714 262 SnaBI TAC'GTA Cuts at: 3201 3201 Size: 9302 SpeI A'CTAG_T Cuts at: 67 5800 67 Size: 5733 3569 SphI G_CATG'C Cuts at: 2534 3159 6355 6855 2534 Size: 625 3196 500 4981 Fragments arranged by size: 4981 3196 625 500 StyI C'CwwG_G Cuts at: 107 471 3805 3853 6436 6790 107 Size: 364 3334 48 2583 354 2619 Fragments arranged by size: 3334 2619 2583 364 354 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3171 3714 6247 6885 7018 7477 7686 3171 Size: 543 2533 638 133 459 209 4787 Fragments arranged by size: 4787 2533 638 543 459 209 133 TatI w'GTAC_w Cuts at: 1543 2635 2851 3375 8817 1543 Size: 1092 216 524 5442 2028 Fragments arranged by size: 5442 2028 1092 524 216 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 338 370 3896 3928 5549 5581 8819 8851 338 Size: 32 3526 32 1621 32 3238 32 789 Fragments arranged by size: 3526 3238 1621 789 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 7099 7099 Size: 9302 XbaI T'CTAG_A Cuts at: 6333 6333 Size: 9302 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6797 6797 Size: 9302 XmaI C'CCGG_G Cuts at: 6322 6322 Size: 9302 XmnI GAAnn'nnTTC Cuts at: 465 1545 1742 3173 5036 5237 465 Size: 1080 197 1431 1863 201 4530 Fragments arranged by size: 4530 1863 1431 1080 201 197 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI PciI PflMI PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI ApaI AscI AvrII BcgI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HpaI NdeI NheI NotI PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI