Restriction map for
pF1KB9494
(Circular) (Six-base) MAPSORT of: pF1KB9494.seq Check: 2476 from: 1 to: 5633
pF1KB9494 5633 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 754 932 2211 754
Size: 178 1279 4176
Fragments arranged by size:
4176 1279 178
AccI GT'mk_AC
Cuts at: 2671 2671
Size: 5633
Acc65I G'GTAC_C
Cuts at: 2649 2649
Size: 5633
AclI AA'CG_TT
Cuts at: 5473 5473
Size: 5633
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 3458 3890 4243 4905 1133
Size: 2325 432 353 662 1861
Fragments arranged by size:
2325 1861 662 432 353
AflIII A'CryG_T
Cuts at: 710 2985 4785 5066 710
Size: 2275 1800 281 1277
Fragments arranged by size:
2275 1800 1277 281
AleI CACnn'nnGTG
Cuts at: 325 325
Size: 5633
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 911 943 911
Size: 32 5601
AlwNI CAG_nnn'CTG
Cuts at: 1560 2397 2436 2463 2553 2556 2938 4376 1560
Size: 837 39 27 90 3 382 1438 2817
Fragments arranged by size:
2817 1438 837 382 90 39 27 3
ApaI G_GGCC'C
Cuts at: 248 1620 248
Size: 1372 4261
ApaLI G'TGCA_C
Cuts at: 2497 4471 2497
Size: 1974 3659
ApoI r'AATT_y
Cuts at: 883 2101 2637 4986 5242 883
Size: 1218 536 2349 256 1274
Fragments arranged by size:
2349 1274 1218 536 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5633
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5633
AvaI C'yCGr_G
Cuts at: 183 1120 1190 2314 2653 183
Size: 937 70 1124 339 3163
Fragments arranged by size:
3163 1124 937 339 70
BamHI G'GATC_C
Cuts at: 2658 2658
Size: 5633
BanI G'GyrC_C
Cuts at: 272 301 1146 1216 2132 2247 2617 2649 3063
Size: 29 845 70 916 115 370 32 414
Cuts at: 3063 3311 3346 272
Size: 248 35 2559
Fragments arranged by size:
2559 916 845 414 370 248 115 70
35 32 29
BanII G_rGCy'C
Cuts at: 248 743 937 1620 2647 3677 248
Size: 495 194 683 1027 1030 2204
Fragments arranged by size:
2204 1030 1027 683 495 194
BbeI G_GCGC'C
Cuts at: 276 2621 3067 3315 276
Size: 2345 446 248 2594
Fragments arranged by size:
2594 2345 446 248
BbsI GAAGACnn'nnnn_
Cuts at: 379 379
Size: 5633
BbvCI CC'TCA_GC
Cuts at: 1224 1224
Size: 5633
BciVI GTATCCnnnnn_n'
Cuts at: 3531 4587 5633 3531
Size: 1056 1046 3531
Fragments arranged by size:
3531 1056 1046
BclI T'GATC_A
Cuts at: 1940 3153 1940
Size: 1213 4420
BglI GCCn_nnn'nGGC
Cuts at: 643 2152 2256 643
Size: 1509 104 4020
Fragments arranged by size:
4020 1509 104
BglII A'GATC_T
Cuts at: 3148 5185 3148
Size: 2037 3596
BlpI GC'TnA_GC
Cuts at: 1879 2542 2745 1879
Size: 663 203 4767
Fragments arranged by size:
4767 663 203
Bme1580I G_kGCm'C
Cuts at: 248 306 1121 1151 1324 1620 2135 2252 2501
Size: 58 815 30 173 296 515 117 249
Cuts at: 2501 3258 3351 4475 248
Size: 757 93 1124 1406
Fragments arranged by size:
1406 1124 815 757 515 296 249 173
117 93 58 30
BmgBI CAC'GTC
Cuts at: 899 1053 1440 899
Size: 154 387 5092
Fragments arranged by size:
5092 387 154
BmrI ACTGGGnnnn_n'
Cuts at: 449 527 1256 1573 2845 2926 3261 5405 449
Size: 78 729 317 1272 81 335 2144 677
Fragments arranged by size:
2144 1272 729 677 335 317 81 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1983 4000 1983
Size: 2017 3616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1582 1813 2104 2157 2419 2458 712
Size: 870 231 291 53 262 39 3887
Fragments arranged by size:
3887 870 291 262 231 53 39
Bpu10I CC'TnA_GC
Cuts at: 1224 1224
Size: 5633
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1873 2809 3688 4402 4700 1873
Size: 936 879 714 298 2806
Fragments arranged by size:
2806 936 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2520 5337 2520
Size: 2817 2816
BsaAI yAC'GTr
Cuts at: 1485 1638 2986 1485
Size: 153 1348 4132
Fragments arranged by size:
4132 1348 153
BsaBI GATnn'nnATC
Cuts at: 6 3147 5184 6
Size: 3141 2037 455
Fragments arranged by size:
3141 2037 455
BsaHI Gr'CG_yC
Cuts at: 273 1301 2618 3064 3312 4014 5510 273
Size: 1028 1317 446 248 702 1496 396
Fragments arranged by size:
1496 1317 1028 702 446 396 248
BsaWI w'CCGG_w
Cuts at: 2195 2822 3343 4021 4432 4579 4853 2195
Size: 627 521 678 411 147 274 2975
Fragments arranged by size:
2975 678 627 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 911 941 1786 1816 911
Size: 30 845 30 4728
Fragments arranged by size:
4728 845 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 769 1156 1180 1480 1959 769
Size: 387 24 300 479 4443
Fragments arranged by size:
4443 479 387 300 24
BseYI C'CCAG_C
Cuts at: 749 1084 1241 1452 2251 2613 3058 3816 4481
Size: 335 157 211 799 362 445 758 665
Cuts at: 4481 4889 749
Size: 408 1493
Fragments arranged by size:
1493 799 758 665 445 408 362 335
211 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 1024 1378 1495 1618 2437 2470 444
Size: 580 354 117 123 819 33 3607
Fragments arranged by size:
3607 819 580 354 123 117 33
BsiEI CG_ry'CG
Cuts at: 104 476 1016 1624 2539 3175 3221 4451 104
Size: 372 540 608 915 636 46 1230 1286
Fragments arranged by size:
1286 1230 915 636 608 540 372 46
BsiHKAI G_wGCw'C
Cuts at: 743 1679 1898 2501 2647 3425 3615 4475 743
Size: 936 219 603 146 778 190 860 1901
Fragments arranged by size:
1901 936 860 778 603 219 190 146
Bsp1286I G_dGCh'C
Cuts at: 248 306 743 937 1121 1151 1324 1620 1679
Size: 58 437 194 184 30 173 296 59
Cuts at: 1679 1898 2135 2252 2501 2647 3258 3351 3425
Size: 219 237 117 249 146 611 93 74
Cuts at: 3425 3615 3677 4475 248
Size: 190 62 798 1406
Fragments arranged by size:
1406 798 611 437 296 249 237 219
194 190 184 173 146 117 93 74
62 59 58 30
BspHI T'CATG_A
Cuts at: 1943 4065 1943
Size: 2122 3511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 754 932 1841 2211 2683 2897 3199 3580 754
Size: 178 909 370 472 214 302 381 2807
Fragments arranged by size:
2807 909 472 381 370 302 214 178
BsrBI CCG'CTC
Cuts at: 50 1170 2092 2171 3925 3979 5463 5628 50
Size: 1120 922 79 1754 54 1484 165 55
Fragments arranged by size:
1754 1484 1120 922 165 79 55 54
BsrDI GCAATG_nn'
Cuts at: 3545 3545
Size: 5633
BsrFI r'CCGG_y
Cuts at: 450 2369 3631 3812 450
Size: 1919 1262 181 2271
Fragments arranged by size:
2271 1919 1262 181
BsrGI T'GTAC_A
Cuts at: 653 1307 1355 1757 653
Size: 654 48 402 4529
Fragments arranged by size:
4529 654 402 48
BssHII G'CGCG_C
Cuts at: 164 164
Size: 5633
BssSI C'ACGA_G
Cuts at: 1396 3904 4612 1396
Size: 2508 708 2417
Fragments arranged by size:
2508 2417 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1842 2424 2484 2556 3010 1842
Size: 582 60 72 454 4465
Fragments arranged by size:
4465 582 454 72 60
BstBI TT'CG_AA
Cuts at: 251 3994 251
Size: 3743 1890
BstYI r'GATC_y
Cuts at: 1 671 776 2432 2658 3125 3148 3483 3729
Size: 670 105 1656 226 467 23 335 246
Cuts at: 3729 4035 4047 4133 5185 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1656 1052 670 467 449 335 306 246
226 105 86 23 12
BtgI C'CryG_G
Cuts at: 107 639 647 803 971 1714 1826 2137 107
Size: 532 8 156 168 743 112 311 3603
Fragments arranged by size:
3603 743 532 311 168 156 112 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 247 3583 3764 3927 247
Size: 3336 181 163 1953
Fragments arranged by size:
3336 1953 181 163
BtsI GCAGTG_nn'
Cuts at: 1041 1896 2478 2608 2876 1041
Size: 855 582 130 268 3798
Fragments arranged by size:
3798 855 582 268 130
DraI TTT'AAA
Cuts at: 2632 2632
Size: 5633
DraIII CAC_nnn'GTG
Cuts at: 324 324
Size: 5633
DrdI GACnn_nn'nnGTC
Cuts at: 3339 4683 3339
Size: 1344 4289
EaeI y'GGCC_r
Cuts at: 473 585 1013 1621 1640 2140 2162 3218 3392
Size: 112 428 608 19 500 22 1056 174
Cuts at: 3392 3783 3810 5155 473
Size: 391 27 1345 951
Fragments arranged by size:
1345 1056 951 608 500 428 391 174
112 27 22 19
EagI C'GGCC_G
Cuts at: 473 1013 1621 3218 473
Size: 540 608 1597 2888
Fragments arranged by size:
2888 1597 608 540
EarI CTCTTCn'nnn_
Cuts at: 877 1279 3656 3866 877
Size: 402 2377 210 2644
Fragments arranged by size:
2644 2377 402 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2013 3841 4583 4729 5443 2013
Size: 1828 742 146 714 2203
Fragments arranged by size:
2203 1828 742 714 146
EcoICRI GAG'CTC
Cuts at: 741 2645 741
Size: 1904 3729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1133 1582 1813 2104 2157 2419 2458 3458
Size: 421 449 231 291 53 262 39 1000
Cuts at: 3458 3890 4243 4905 712
Size: 432 353 662 1440
Fragments arranged by size:
1440 1000 662 449 432 421 353 291
262 231 53 39
EcoNI CCTnn'n_nnAGG
Cuts at: 423 423
Size: 5633
EcoO109I rG'GnC_Cy
Cuts at: 245 428 443 1593 1616 2772 245
Size: 183 15 1150 23 1156 3106
Fragments arranged by size:
3106 1156 1150 183 23 15
EcoRI G'AATT_C
Cuts at: 883 2101 2637 883
Size: 1218 536 3879
Fragments arranged by size:
3879 1218 536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3652 3652
Size: 5633
FspI TGC'GCA
Cuts at: 2607 3414 2607
Size: 807 4826
HaeII r_GCGC'y
Cuts at: 276 960 1965 2621 3067 3315 4545 5316 276
Size: 684 1005 656 446 248 1230 771 593
Fragments arranged by size:
1230 1005 771 684 656 593 446 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3475 3505 3507 3537 3475
Size: 30 2 30 5571
Fragments arranged by size:
5571 30 30 2
HincII GTy'rAC
Cuts at: 2304 2672 2304
Size: 368 5265
KasI G'GCGC_C
Cuts at: 272 2617 3063 3311 272
Size: 2345 446 248 2594
Fragments arranged by size:
2594 2345 446 248
KpnI G_GTAC'C
Cuts at: 2653 2653
Size: 5633
MfeI C'AATT_G
Cuts at: 719 719
Size: 5633
MluI A'CGCG_T
Cuts at: 5066 5066
Size: 5633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1115 4392 4576 1115
Size: 3277 184 2172
Fragments arranged by size:
3277 2172 184
MscI TGG'CCA
Cuts at: 587 1642 3394 587
Size: 1055 1752 2826
Fragments arranged by size:
2826 1752 1055
MslI CAynn'nnrTG
Cuts at: 325 995 1046 1753 2494 3622 3749 325
Size: 670 51 707 741 1128 127 2209
Fragments arranged by size:
2209 1128 741 707 670 127 51
MspA1I CmG'CkG
Cuts at: 954 1346 1511 1800 1878 2446 2553 2744 3058
Size: 392 165 289 78 568 107 191 314
Cuts at: 3058 3418 4202 4447 954
Size: 360 784 245 2140
Fragments arranged by size:
2140 784 568 392 360 314 289 245
191 165 107 78
NaeI GCC'GGC
Cuts at: 3814 3814
Size: 5633
NarI GG'CG_CC
Cuts at: 273 2618 3064 3312 273
Size: 2345 446 248 2594
Fragments arranged by size:
2594 2345 446 248
NcoI C'CATG_G
Cuts at: 107 639 971 107
Size: 532 332 4769
Fragments arranged by size:
4769 532 332
NgoMIV G'CCGG_C
Cuts at: 3812 3812
Size: 5633
NspI r_CATG'y
Cuts at: 498 714 2310 2686 3186 4789 498
Size: 216 1596 376 500 1603 1342
Fragments arranged by size:
1603 1596 1342 500 376 216
PasI CC'CwG_GG
Cuts at: 1586 1586
Size: 5633
PciI A'CATG_T
Cuts at: 710 4785 710
Size: 4075 1558
PflMI CCAn_nnn'nTGG
Cuts at: 1560 2040 1560
Size: 480 5153
PfoI T'CCnGG_A
Cuts at: 728 1646 2063 2435 728
Size: 918 417 372 3926
Fragments arranged by size:
3926 918 417 372
PmeI GTTT'AAAC
Cuts at: 2632 2632
Size: 5633
PmlI CAC'GTG
Cuts at: 1638 1638
Size: 5633
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 911 943 4079 4111 911
Size: 32 3136 32 2433
Fragments arranged by size:
3136 2433 32 32
PpuMI rG'GwC_Cy
Cuts at: 443 443
Size: 5633
PspOMI G'GGCC_C
Cuts at: 244 1616 244
Size: 1372 4261
PstI C_TGCA'G
Cuts at: 1838 1877 2378 2432 2680 3365 1838
Size: 39 501 54 248 685 4106
Fragments arranged by size:
4106 685 501 248 54 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5633
PvuII CAG'CTG
Cuts at: 1800 1878 2446 2553 3058 3418 1800
Size: 78 568 107 505 360 4015
Fragments arranged by size:
4015 568 505 360 107 78
RsrII CG'GwC_CG
Cuts at: 3828 3828
Size: 5633
SacI G_AGCT'C
Cuts at: 743 2647 743
Size: 1904 3729
SalI G'TCGA_C
Cuts at: 2670 2670
Size: 5633
SanDI GG'GwC_CC
Cuts at: 443 443
Size: 5633
SapI GCTCTTCn'nnn_
Cuts at: 1279 3656 3866 1279
Size: 2377 210 3046
Fragments arranged by size:
3046 2377 210
SbfI CC_TGCA'GG
Cuts at: 2680 2680
Size: 5633
ScaI AGT'ACT
Cuts at: 5150 5150
Size: 5633
SexAI A'CCwGG_T
Cuts at: 1934 1934
Size: 5633
SfcI C'TryA_G
Cuts at: 33 1834 1873 2374 2428 2676 3361 4329 4520
Size: 1801 39 501 54 248 685 968 191
Cuts at: 4520 33
Size: 1146
Fragments arranged by size:
1801 1146 968 685 501 248 191 54
39
SfoI GGC'GCC
Cuts at: 274 2619 3065 3313 274
Size: 2345 446 248 2594
Fragments arranged by size:
2594 2345 446 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5633
SmaI CCC'GGG
Cuts at: 2655 2655
Size: 5633
SmlI C'TyrA_G
Cuts at: 1888 2788 3703 4417 4679 1888
Size: 900 915 714 262 2842
Fragments arranged by size:
2842 915 900 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5633
SphI G_CATG'C
Cuts at: 2686 3186 2686
Size: 500 5133
StuI AGG'CCT
Cuts at: 1822 2263 1822
Size: 441 5192
StyI C'CwwG_G
Cuts at: 107 173 639 971 2027 2510 2767 3121 107
Size: 66 466 332 1056 483 257 354 2619
Fragments arranged by size:
2619 1056 483 466 354 332 257 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 289 749 1255 1444 2150 3216 3349 3808 4017
Size: 460 506 189 706 1066 133 459 209
Cuts at: 4017 289
Size: 1905
Fragments arranged by size:
1905 1066 706 506 460 459 209 189
133
TatI w'GTAC_w
Cuts at: 653 1307 1355 1757 1811 5148 653
Size: 654 48 402 54 3337 1138
Fragments arranged by size:
3337 1138 654 402 54 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 883 915 1760 1786 1792 1818 5150 5182 883
Size: 32 845 26 6 26 3332 32 1334
Fragments arranged by size:
3332 1334 845 32 32 26 26 6
Tth111I GACn'n_nGTC
Cuts at: 217 3430 217
Size: 3213 2420
XbaI T'CTAG_A
Cuts at: 2664 2664
Size: 5633
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2241 3128 2241
Size: 887 4746
XmaI C'CCGG_G
Cuts at: 2653 2653
Size: 5633
XmnI GAAnn'nnTTC
Cuts at: 1954 1954
Size: 5633
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BspEI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI