Restriction map for
pF1KB9504
(Circular) (Six-base) MAPSORT of: pF1KB9504.seq Check: 3308 from: 1 to: 7055
pF1KB9504 7055 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1949 3334 1949
Size: 1385 5670
AccI GT'mk_AC
Cuts at: 2594 4093 2594
Size: 1499 5556
Acc65I G'GTAC_C
Cuts at: 3047 4071 3047
Size: 1024 6031
AclI AA'CG_TT
Cuts at: 6895 6895
Size: 7055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1651 1833 4880 5312 5665 6327 1651
Size: 182 3047 432 353 662 2379
Fragments arranged by size:
3047 2379 662 432 353 182
AfeI AGC'GCT
Cuts at: 3733 3733
Size: 7055
AflIII A'CryG_T
Cuts at: 2894 4407 6207 6488 2894
Size: 1513 1800 281 3461
Fragments arranged by size:
3461 1800 1513 281
AleI CACnn'nnGTG
Cuts at: 493 3379 3625 3991 493
Size: 2886 246 366 3557
Fragments arranged by size:
3557 2886 366 246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 655 1384 1726 3592 3660 3727 3767 655
Size: 729 342 1866 68 67 40 3943
Fragments arranged by size:
3943 1866 729 342 68 67 40
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1604 1636 1604
Size: 32 7023
AlwNI CAG_nnn'CTG
Cuts at: 169 297 1191 1629 1920 2082 2173 2279 2403
Size: 128 894 438 291 162 91 106 124
Cuts at: 2403 2883 3462 3585 3700 3721 3798 3835 3951
Size: 480 579 123 115 21 77 37 116
Cuts at: 3951 4360 5798 169
Size: 409 1438 1426
Fragments arranged by size:
1438 1426 894 579 480 438 409 291
162 128 124 123 116 115 106 91
77 37 21
ApaI G_GGCC'C
Cuts at: 3000 3000
Size: 7055
ApaLI G'TGCA_C
Cuts at: 561 1180 2758 5893 561
Size: 619 1578 3135 1723
Fragments arranged by size:
3135 1723 1578 619
ApoI r'AATT_y
Cuts at: 3235 4059 6408 6664 3235
Size: 824 2349 256 3626
Fragments arranged by size:
3626 2349 824 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7055
AvaI C'yCGr_G
Cuts at: 2311 2695 2935 4075 2311
Size: 384 240 1140 5291
Fragments arranged by size:
5291 1140 384 240
BamHI G'GATC_C
Cuts at: 3011 4080 3011
Size: 1069 5986
BanI G'GyrC_C
Cuts at: 472 2483 2973 3047 3183 3381 4071 4485 4733
Size: 2011 490 74 136 198 690 414 248
Cuts at: 4733 4768 472
Size: 35 2759
Fragments arranged by size:
2759 2011 690 490 414 248 198 136
74 35
BanII G_rGCy'C
Cuts at: 545 1015 1429 1463 2006 2777 3000 3692 4069
Size: 470 414 34 543 771 223 692 377
Cuts at: 4069 5099 545
Size: 1030 2501
Fragments arranged by size:
2501 1030 771 692 543 470 414 377
223 34
BbeI G_GCGC'C
Cuts at: 2977 4489 4737 2977
Size: 1512 248 5295
Fragments arranged by size:
5295 1512 248
BbsI GAAGACnn'nnnn_
Cuts at: 1401 1872 1954 1401
Size: 471 82 6502
Fragments arranged by size:
6502 471 82
BbvCI CC'TCA_GC
Cuts at: 2161 2386 4041 2161
Size: 225 1655 5175
Fragments arranged by size:
5175 1655 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2647 2681 2647
Size: 34 7021
BciVI GTATCCnnnnn_n'
Cuts at: 4953 6009 7055 4953
Size: 1056 1046 4953
Fragments arranged by size:
4953 1056 1046
BclI T'GATC_A
Cuts at: 4575 4575
Size: 7055
BglI GCCn_nnn'nGGC
Cuts at: 1925 2121 1925
Size: 196 6859
BglII A'GATC_T
Cuts at: 4570 6607 4570
Size: 2037 5018
BlpI GC'TnA_GC
Cuts at: 1372 2236 3272 4167 1372
Size: 864 1036 895 4260
Fragments arranged by size:
4260 1036 895 864
Bme1580I G_kGCm'C
Cuts at: 565 766 933 1156 1184 2488 2762 2854 3000
Size: 201 167 223 28 1304 274 92 146
Cuts at: 3000 4680 4773 5897 565
Size: 1680 93 1124 1723
Fragments arranged by size:
1723 1680 1304 1124 274 223 201 167
146 93 92 28
BmgBI CAC'GTC
Cuts at: 1392 1392
Size: 7055
BmrI ACTGGGnnnn_n'
Cuts at: 769 871 3231 4017 4267 4348 4683 6827 769
Size: 102 2360 786 250 81 335 2144 997
Fragments arranged by size:
2360 2144 997 786 335 250 102 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5422 5422
Size: 7055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1476 1990 2020 3048 3097 3301 1476
Size: 514 30 1028 49 204 5230
Fragments arranged by size:
5230 1028 514 204 49 30
Bpu10I CC'TnA_GC
Cuts at: 1585 2161 2321 2386 2560 2707 4041 1585
Size: 576 160 65 174 147 1334 4599
Fragments arranged by size:
4599 1334 576 174 160 147 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2722 3109 3295 4231 5110 5824 6122 1225
Size: 1497 387 186 936 879 714 298 2158
Fragments arranged by size:
2158 1497 936 879 714 387 298 186
BsaI GGTCTCn'nnnn_
Cuts at: 1525 2681 2785 6759 1525
Size: 1156 104 3974 1821
Fragments arranged by size:
3974 1821 1156 104
BsaAI yAC'GTr
Cuts at: 2895 4408 2895
Size: 1513 5542
BsaBI GATnn'nnATC
Cuts at: 6 3370 4569 6606 6
Size: 3364 1199 2037 455
Fragments arranged by size:
3364 2037 1199 455
BsaHI Gr'CG_yC
Cuts at: 2974 3095 3435 4486 4734 5436 6932 2974
Size: 121 340 1051 248 702 1496 3097
Fragments arranged by size:
3097 1496 1051 702 340 248 121
BsaWI w'CCGG_w
Cuts at: 3725 4244 4765 5443 5854 6001 6275 3725
Size: 519 521 678 411 147 274 4505
Fragments arranged by size:
4505 678 521 519 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1988 1993 2018 2023 2893 2923 3021 3051 1988
Size: 5 25 5 870 30 98 30 5992
Fragments arranged by size:
5992 870 98 30 30 25 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 355 522 604 1039 1456 1543 1555 1870
Size: 3 167 82 435 417 87 12 315
Cuts at: 1870 1921 2056 2329 2508 2785 2953 3871 4029
Size: 51 135 273 179 277 168 918 158
Cuts at: 4029 352
Size: 3378
Fragments arranged by size:
3378 918 435 417 315 277 273 179
168 167 158 135 87 82 51 12
3
BseYI C'CCAG_C
Cuts at: 258 444 810 942 1134 2052 2063 2298 2403
Size: 186 366 132 192 918 11 235 105
Cuts at: 2403 3106 3187 3597 3693 4480 5238 5903 6311
Size: 703 81 410 96 787 758 665 408
Cuts at: 6311 258
Size: 1002
Fragments arranged by size:
1002 918 787 758 703 665 410 408
366 235 192 186 132 105 96 81
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 3598 217
Size: 3381 3674
BsiEI CG_ry'CG
Cuts at: 104 4597 4643 5873 104
Size: 4493 46 1230 1286
Fragments arranged by size:
4493 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 565 1015 1121 1184 1397 1463 1516 2006 2209
Size: 450 106 63 213 66 53 490 203
Cuts at: 2209 2660 2736 2762 2777 3632 4069 4847 5037
Size: 451 76 26 15 855 437 778 190
Cuts at: 5037 5897 565
Size: 860 1723
Fragments arranged by size:
1723 860 855 778 490 451 450 437
213 203 190 106 76 66 63 53
26 15
Bsp1286I G_dGCh'C
Cuts at: 545 565 766 933 1015 1121 1156 1184 1397
Size: 20 201 167 82 106 35 28 213
Cuts at: 1397 1429 1463 1516 2006 2209 2488 2660 2736
Size: 32 34 53 490 203 279 172 76
Cuts at: 2736 2762 2777 2854 3000 3632 3692 4069 4680
Size: 26 15 77 146 632 60 377 611
Cuts at: 4680 4773 4847 5037 5099 5897 545
Size: 93 74 190 62 798 1703
Fragments arranged by size:
1703 798 632 611 490 377 279 213
203 201 190 172 167 146 106 93
82 77 76 74 62 60 53 35
34 32 28 26 20 15
BspHI T'CATG_A
Cuts at: 1121 2183 2372 5487 1121
Size: 1062 189 3115 2689
Fragments arranged by size:
3115 2689 1062 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 449 1328 1919 1949 3334 4105 4319 4621
Size: 144 879 591 30 1385 771 214 302
Cuts at: 4621 5002 305
Size: 381 2358
Fragments arranged by size:
2358 1385 879 771 591 381 302 214
144 30
BsrBI CCG'CTC
Cuts at: 50 482 1400 1545 3285 5347 5401 6885 7050
Size: 432 918 145 1740 2062 54 1484 165
Cuts at: 7050 50
Size: 55
Fragments arranged by size:
2062 1740 1484 918 432 165 145 55
54
BsrDI GCAATG_nn'
Cuts at: 1383 4967 1383
Size: 3584 3471
BsrFI r'CCGG_y
Cuts at: 124 890 1447 1784 2480 3302 5053 5234 124
Size: 766 557 337 696 822 1751 181 1945
Fragments arranged by size:
1945 1751 822 766 696 557 337 181
BsrGI T'GTAC_A
Cuts at: 497 2378 497
Size: 1881 5174
BssSI C'ACGA_G
Cuts at: 5326 6034 5326
Size: 708 6347
BstAPI GCAn_nnn'nTGC
Cuts at: 3835 4432 3835
Size: 597 6458
BstBI TT'CG_AA
Cuts at: 5416 5416
Size: 7055
BstEII G'GTnAC_C
Cuts at: 3504 3504
Size: 7055
BstXI CCAn_nnnn'nTGG
Cuts at: 792 3555 3913 792
Size: 2763 358 3934
Fragments arranged by size:
3934 2763 358
BstYI r'GATC_y
Cuts at: 1 1310 1625 3011 3257 3639 4080 4547 4570
Size: 1309 315 1386 246 382 441 467 23
Cuts at: 4570 4905 5151 5457 5469 5555 6607 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1386 1309 1052 467 449 441 382 335
315 306 246 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1342 1342
Size: 7055
BtgI C'CryG_G
Cuts at: 107 839 1175 2018 2191 2954 107
Size: 732 336 843 173 763 4208
Fragments arranged by size:
4208 843 763 732 336 173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5005 5186 5349 5005
Size: 181 163 6711
Fragments arranged by size:
6711 181 163
BtsI GCAGTG_nn'
Cuts at: 764 965 1485 2098 3566 3704 4298 764
Size: 201 520 613 1468 138 594 3521
Fragments arranged by size:
3521 1468 613 594 520 201 138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3312 3347 3312
Size: 35 7020
DraI TTT'AAA
Cuts at: 4054 4054
Size: 7055
DraIII CAC_nnn'GTG
Cuts at: 913 1478 913
Size: 565 6490
DrdI GACnn_nn'nnGTC
Cuts at: 3340 4761 6105 3340
Size: 1421 1344 4290
Fragments arranged by size:
4290 1421 1344
EaeI y'GGCC_r
Cuts at: 1728 2714 2885 3915 4640 4814 5205 5232 6577
Size: 986 171 1030 725 174 391 27 1345
Cuts at: 6577 1728
Size: 2206
Fragments arranged by size:
2206 1345 1030 986 725 391 174 171
27
EagI C'GGCC_G
Cuts at: 4640 4640
Size: 7055
EarI CTCTTCn'nnn_
Cuts at: 246 1213 1390 2783 5078 5288 246
Size: 967 177 1393 2295 210 2013
Fragments arranged by size:
2295 2013 1393 967 210 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2467 3169 5263 6005 6151 6865 2467
Size: 702 2094 742 146 714 2657
Fragments arranged by size:
2657 2094 742 714 702 146
EcoICRI GAG'CTC
Cuts at: 1013 1461 2004 2775 4067 1013
Size: 448 543 771 1292 4001
Fragments arranged by size:
4001 1292 771 543 448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1476 1651 1833 1990 2020 3048 3097 3301 4880
Size: 175 182 157 30 1028 49 204 1579
Cuts at: 4880 5312 5665 6327 1476
Size: 432 353 662 2204
Fragments arranged by size:
2204 1579 1028 662 432 353 204 182
175 157 49 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2032 3166 2032
Size: 1134 5921
EcoO109I rG'GnC_Cy
Cuts at: 522 789 903 1188 1926 2079 2252 2880 3172
Size: 267 114 285 738 153 173 628 292
Cuts at: 3172 3861 4194 522
Size: 689 333 3383
Fragments arranged by size:
3383 738 689 628 333 292 285 267
173 153 114
EcoRI G'AATT_C
Cuts at: 3235 4059 3235
Size: 824 6231
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 388 2763 3987 5074 388
Size: 2375 1224 1087 2369
Fragments arranged by size:
2375 2369 1224 1087
FseI GG_CCGG'CC
Cuts at: 1451 1451
Size: 7055
FspI TGC'GCA
Cuts at: 643 1093 4836 643
Size: 450 3743 2862
Fragments arranged by size:
3743 2862 450
HaeII r_GCGC'y
Cuts at: 1920 2070 2977 3279 3735 4489 4737 5967 6738
Size: 150 907 302 456 754 248 1230 771
Cuts at: 6738 1920
Size: 2237
Fragments arranged by size:
2237 1230 907 771 754 456 302 248
150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 766 798 1242 1274 1993 2025 2553 2585 4897
Size: 32 444 32 719 32 528 32 2312
Cuts at: 4897 4927 4929 4959 766
Size: 30 2 30 2862
Fragments arranged by size:
2862 2312 719 528 444 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2012 2519 4094 2012
Size: 507 1575 4973
Fragments arranged by size:
4973 1575 507
HindIII A'AGCT_T
Cuts at: 1670 1670
Size: 7055
KasI G'GCGC_C
Cuts at: 2973 4485 4733 2973
Size: 1512 248 5295
Fragments arranged by size:
5295 1512 248
KpnI G_GTAC'C
Cuts at: 3051 4075 3051
Size: 1024 6031
MluI A'CGCG_T
Cuts at: 6488 6488
Size: 7055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 579 980 2158 5814 5998 579
Size: 401 1178 3656 184 1636
Fragments arranged by size:
3656 1636 1178 401 184
MscI TGG'CCA
Cuts at: 2716 2887 3917 4816 2716
Size: 171 1030 899 4955
Fragments arranged by size:
4955 1030 899 171
MslI CAynn'nnrTG
Cuts at: 493 1519 1600 1744 2282 3244 3379 3553 3574
Size: 1026 81 144 538 962 135 174 21
Cuts at: 3574 3625 3991 5044 5171 493
Size: 51 366 1053 127 2377
Fragments arranged by size:
2377 1053 1026 962 538 366 174 144
135 127 81 51 21
MspA1I CmG'CkG
Cuts at: 572 688 1581 2170 2403 2988 3447 3478 3835
Size: 116 893 589 233 585 459 31 357
Cuts at: 3835 4045 4166 4480 4840 5624 5869 572
Size: 210 121 314 360 784 245 1758
Fragments arranged by size:
1758 893 784 589 585 459 360 357
314 245 233 210 121 116 31
NaeI GCC'GGC
Cuts at: 126 1449 3304 5236 126
Size: 1323 1855 1932 1945
Fragments arranged by size:
1945 1932 1855 1323
NarI GG'CG_CC
Cuts at: 2974 4486 4734 2974
Size: 1512 248 5295
Fragments arranged by size:
5295 1512 248
NcoI C'CATG_G
Cuts at: 107 2018 2954 107
Size: 1911 936 4208
Fragments arranged by size:
4208 1911 936
NgoMIV G'CCGG_C
Cuts at: 124 1447 3302 5234 124
Size: 1323 1855 1932 1945
Fragments arranged by size:
1945 1932 1855 1323
NspI r_CATG'y
Cuts at: 132 253 494 1743 4108 4608 6211 132
Size: 121 241 1249 2365 500 1603 976
Fragments arranged by size:
2365 1603 1249 976 500 241 121
PasI CC'CwG_GG
Cuts at: 537 2993 537
Size: 2456 4599
PciI A'CATG_T
Cuts at: 6207 6207
Size: 7055
PflMI CCAn_nnn'nTGG
Cuts at: 1191 1475 1478 2403 3700 1191
Size: 284 3 925 1297 4546
Fragments arranged by size:
4546 1297 925 284 3
PfoI T'CCnGG_A
Cuts at: 392 1190 2357 392
Size: 798 1167 5090
Fragments arranged by size:
5090 1167 798
PmeI GTTT'AAAC
Cuts at: 4054 4054
Size: 7055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5501 5533 5501
Size: 32 7023
PpuMI rG'GwC_Cy
Cuts at: 522 903 1188 2252 3172 3861 522
Size: 381 285 1064 920 689 3716
Fragments arranged by size:
3716 1064 920 689 381 285
PspOMI G'GGCC_C
Cuts at: 2996 2996
Size: 7055
PstI C_TGCA'G
Cuts at: 902 1457 1715 1916 3143 3746 4102 4787 902
Size: 555 258 201 1227 603 356 685 3170
Fragments arranged by size:
3170 1227 685 603 555 356 258 201
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7055
PvuII CAG'CTG
Cuts at: 572 2403 3447 3478 3835 4045 4480 4840 572
Size: 1831 1044 31 357 210 435 360 2787
Fragments arranged by size:
2787 1831 1044 435 360 357 210 31
RsrII CG'GwC_CG
Cuts at: 5250 5250
Size: 7055
SacI G_AGCT'C
Cuts at: 1015 1463 2006 2777 4069 1015
Size: 448 543 771 1292 4001
Fragments arranged by size:
4001 1292 771 543 448
SalI G'TCGA_C
Cuts at: 4092 4092
Size: 7055
SapI GCTCTTCn'nnn_
Cuts at: 1213 5078 5288 1213
Size: 3865 210 2980
Fragments arranged by size:
3865 2980 210
SbfI CC_TGCA'GG
Cuts at: 902 1457 4102 902
Size: 555 2645 3855
Fragments arranged by size:
3855 2645 555
ScaI AGT'ACT
Cuts at: 4032 6572 4032
Size: 2540 4515
SexAI A'CCwGG_T
Cuts at: 920 1184 1474 3455 920
Size: 264 290 1981 4520
Fragments arranged by size:
4520 1981 290 264
SfcI C'TryA_G
Cuts at: 33 150 322 898 1306 1453 1615 1711 1912
Size: 117 172 576 408 147 162 96 201
Cuts at: 1912 2727 3139 3163 3742 3998 4098 4783 5751
Size: 815 412 24 579 256 100 685 968
Cuts at: 5751 5942 33
Size: 191 1146
Fragments arranged by size:
1146 968 815 685 579 576 412 408
256 201 191 172 162 147 117 100
96 24
SfoI GGC'GCC
Cuts at: 2975 4487 4735 2975
Size: 1512 248 5295
Fragments arranged by size:
5295 1512 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7055
SgrAI Cr'CCGG_yG
Cuts at: 2480 2480
Size: 7055
SmaI CCC'GGG
Cuts at: 4077 4077
Size: 7055
SmlI C'TyrA_G
Cuts at: 1240 2737 3088 3310 4210 5125 5839 6101 1240
Size: 1497 351 222 900 915 714 262 2194
Fragments arranged by size:
2194 1497 915 900 714 351 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7055
SphI G_CATG'C
Cuts at: 4108 4608 4108
Size: 500 6555
StuI AGG'CCT
Cuts at: 1861 1861
Size: 7055
StyI C'CwwG_G
Cuts at: 107 116 2018 2954 4189 4543 107
Size: 9 1902 936 1235 354 2619
Fragments arranged by size:
2619 1902 1235 936 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1173 1299 2407 3201 3330 4638 4771 5230 5439
Size: 126 1108 794 129 1308 133 459 209
Cuts at: 5439 1173
Size: 2789
Fragments arranged by size:
2789 1308 1108 794 459 209 133 129
126
TatI w'GTAC_w
Cuts at: 497 1763 2378 4030 6570 497
Size: 1266 615 1652 2540 982
Fragments arranged by size:
2540 1652 1266 982 615
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1186 1218 1274 1306 1988 2020 3021 3053 3176
Size: 32 56 32 682 32 1001 32 123
Cuts at: 3176 3208 6572 6604 1186
Size: 32 3364 32 1637
Fragments arranged by size:
3364 1637 1001 682 123 56 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4852 4852
Size: 7055
XbaI T'CTAG_A
Cuts at: 4086 4086
Size: 7055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 390 2079 3012 4550 390
Size: 1689 933 1538 2895
Fragments arranged by size:
2895 1689 1538 933
XmaI C'CCGG_G
Cuts at: 4075 4075
Size: 7055
XmnI GAAnn'nnTTC
Cuts at: 2536 3733 2536
Size: 1197 5858
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BsmBI BspEI BssHII BstZ17I ClaI
EcoRV FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SnaBI SrfI SspI SwaI XhoI ZraI