Restriction map for
pF1KB9506
(Circular) (Six-base) MAPSORT of: pF1KB9506.seq Check: 3189 from: 1 to: 4439
pF1KB9506 4439 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 447 447
Size: 4439
AccI GT'mk_AC
Cuts at: 1477 1477
Size: 4439
Acc65I G'GTAC_C
Cuts at: 1455 1455
Size: 4439
AclI AA'CG_TT
Cuts at: 4279 4279
Size: 4439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 2264 2696 3049 3711 387
Size: 1877 432 353 662 1115
Fragments arranged by size:
1877 1115 662 432 353
AfeI AGC'GCT
Cuts at: 253 253
Size: 4439
AflIII A'CryG_T
Cuts at: 1791 3591 3872 1791
Size: 1800 281 2358
Fragments arranged by size:
2358 1800 281
AleI CACnn'nnGTG
Cuts at: 967 967
Size: 4439
AlwNI CAG_nnn'CTG
Cuts at: 646 1744 3182 646
Size: 1098 1438 1903
Fragments arranged by size:
1903 1438 1098
ApaI G_GGCC'C
Cuts at: 182 189 249 1046 1180 182
Size: 7 60 797 134 3441
Fragments arranged by size:
3441 797 134 60 7
ApaLI G'TGCA_C
Cuts at: 3277 3277
Size: 4439
ApoI r'AATT_y
Cuts at: 1443 3792 4048 1443
Size: 2349 256 1834
Fragments arranged by size:
2349 1834 256
AscI GG'CGCG_CC
Cuts at: 234 234
Size: 4439
AseI AT'TA_AT
Cuts at: 20 20
Size: 4439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
AvaI C'yCGr_G
Cuts at: 181 345 357 787 1173 1206 1459 181
Size: 164 12 430 386 33 253 3161
Fragments arranged by size:
3161 430 386 253 164 33 12
BamHI G'GATC_C
Cuts at: 1464 1464
Size: 4439
BanI G'GyrC_C
Cuts at: 154 792 1165 1294 1455 1869 2117 2152 154
Size: 638 373 129 161 414 248 35 2441
Fragments arranged by size:
2441 638 414 373 248 161 129 35
BanII G_rGCy'C
Cuts at: 182 189 249 298 358 494 563 882 1046
Size: 7 60 49 60 136 69 319 164
Cuts at: 1046 1180 1453 2483 182
Size: 134 273 1030 2138
Fragments arranged by size:
2138 1030 319 273 164 136 134 69
60 60 49 7
BbeI G_GCGC'C
Cuts at: 158 796 1298 1873 2121 158
Size: 638 502 575 248 2476
Fragments arranged by size:
2476 638 575 502 248
BciVI GTATCCnnnnn_n'
Cuts at: 1252 2337 3393 4439 1252
Size: 1085 1056 1046 1252
Fragments arranged by size:
1252 1085 1056 1046
BclI T'GATC_A
Cuts at: 1959 1959
Size: 4439
BglI GCCn_nnn'nGGC
Cuts at: 148 244 655 148
Size: 96 411 3932
Fragments arranged by size:
3932 411 96
BglII A'GATC_T
Cuts at: 1954 3991 1954
Size: 2037 2402
BlpI GC'TnA_GC
Cuts at: 1215 1223 1551 1215
Size: 8 328 4103
Fragments arranged by size:
4103 328 8
Bme1580I G_kGCm'C
Cuts at: 182 189 249 478 1046 1170 1180 2064 2157
Size: 7 60 229 568 124 10 884 93
Cuts at: 2157 3281 182
Size: 1124 1340
Fragments arranged by size:
1340 1124 884 568 229 124 93 60
10 7
BmrI ACTGGGnnnn_n'
Cuts at: 1040 1651 1732 2067 4211 1040
Size: 611 81 335 2144 1268
Fragments arranged by size:
2144 1268 611 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 513 813 2806 513
Size: 300 1993 2146
Fragments arranged by size:
2146 1993 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 535 971 1267 1390 310
Size: 225 436 296 123 3359
Fragments arranged by size:
3359 436 296 225 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 2494 3208 3506 1615
Size: 879 714 298 2548
Fragments arranged by size:
2548 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 270 985 1069 4143 270
Size: 715 84 3074 566
Fragments arranged by size:
3074 715 566 84
BsaAI yAC'GTr
Cuts at: 1792 1792
Size: 4439
BsaBI GATnn'nnATC
Cuts at: 6 1953 3990 6
Size: 1947 2037 455
Fragments arranged by size:
2037 1947 455
BsaHI Gr'CG_yC
Cuts at: 155 793 1295 1870 2118 2820 4316 155
Size: 638 502 575 248 702 1496 278
Fragments arranged by size:
1496 702 638 575 502 278 248
BsaWI w'CCGG_w
Cuts at: 1029 1628 2149 2827 3238 3385 3659 1029
Size: 599 521 678 411 147 274 1809
Fragments arranged by size:
1809 678 599 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 969 999 969
Size: 30 4409
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 939 1435 939
Size: 496 3943
BseYI C'CCAG_C
Cuts at: 248 562 670 1864 2622 3287 3695 248
Size: 314 108 1194 758 665 408 992
Fragments arranged by size:
1194 992 758 665 408 314 108
BsiEI CG_ry'CG
Cuts at: 104 176 1981 2027 3257 104
Size: 72 1805 46 1230 1286
Fragments arranged by size:
1805 1286 1230 72 46
BsiHKAI G_wGCw'C
Cuts at: 494 931 1265 1453 2231 2421 3281 494
Size: 437 334 188 778 190 860 1652
Fragments arranged by size:
1652 860 778 437 334 190 188
BsiWI C'GTAC_G
Cuts at: 729 729
Size: 4439
BsmBI CGTCTCn'nnnn_
Cuts at: 588 588
Size: 4439
Bsp1286I G_dGCh'C
Cuts at: 182 189 249 298 358 478 494 563 882
Size: 7 60 49 60 120 16 69 319
Cuts at: 882 931 1046 1170 1180 1265 1453 2064 2157
Size: 49 115 124 10 85 188 611 93
Cuts at: 2157 2231 2421 2483 3281 182
Size: 74 190 62 798 1340
Fragments arranged by size:
1340 798 611 319 190 188 124 120
115 93 85 74 69 62 60 60
49 49 16 10 7
BspHI T'CATG_A
Cuts at: 2871 2871
Size: 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 447 1489 1703 2005 2386 447
Size: 1042 214 302 381 2500
Fragments arranged by size:
2500 1042 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1251 2731 2785 4269 4434 50
Size: 1201 1480 54 1484 165 55
Fragments arranged by size:
1484 1480 1201 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2351 2351
Size: 4439
BsrFI r'CCGG_y
Cuts at: 151 891 2437 2618 151
Size: 740 1546 181 1972
Fragments arranged by size:
1972 1546 740 181
BssHII G'CGCG_C
Cuts at: 234 234
Size: 4439
BssSI C'ACGA_G
Cuts at: 2710 3418 2710
Size: 708 3731
BstAPI GCAn_nnn'nTGC
Cuts at: 1816 1816
Size: 4439
BstBI TT'CG_AA
Cuts at: 2800 2800
Size: 4439
BstXI CCAn_nnnn'nTGG
Cuts at: 534 594 969 1104 534
Size: 60 375 135 3869
Fragments arranged by size:
3869 375 135 60
BstYI r'GATC_y
Cuts at: 1 1464 1931 1954 2289 2535 2841 2853 2939
Size: 1463 467 23 335 246 306 12 86
Cuts at: 2939 3991 1
Size: 1052 449
Fragments arranged by size:
1463 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 144 170 189 239 281 593 809 144
Size: 26 19 50 42 312 216 3774
Fragments arranged by size:
3774 312 216 50 42 26 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2389 2570 2733 2389
Size: 181 163 4095
Fragments arranged by size:
4095 181 163
BtsI GCAGTG_nn'
Cuts at: 267 602 704 947 1153 1321 1682 267
Size: 335 102 243 206 168 361 3024
Fragments arranged by size:
3024 361 335 243 206 168 102
DraI TTT'AAA
Cuts at: 1438 1438
Size: 4439
DrdI GACnn_nn'nnGTC
Cuts at: 2145 3489 2145
Size: 1344 3095
EaeI y'GGCC_r
Cuts at: 173 635 656 698 871 893 1127 2024 2198
Size: 462 21 42 173 22 234 897 174
Cuts at: 2198 2589 2616 3961 173
Size: 391 27 1345 651
Fragments arranged by size:
1345 897 651 462 391 234 174 173
42 27 22 21
EagI C'GGCC_G
Cuts at: 173 2024 173
Size: 1851 2588
EarI CTCTTCn'nnn_
Cuts at: 482 551 1067 1231 2462 2672 482
Size: 69 516 164 1231 210 2249
Fragments arranged by size:
2249 1231 516 210 164 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2647 3389 3535 4249 2647
Size: 742 146 714 2837
Fragments arranged by size:
2837 742 714 146
EcoICRI GAG'CTC
Cuts at: 492 1451 492
Size: 959 3480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 387 535 971 1267 1390 2264 2696 3049
Size: 77 148 436 296 123 874 432 353
Cuts at: 3049 3711 310
Size: 662 1038
Fragments arranged by size:
1038 874 662 436 432 353 296 148
123 77
EcoO109I rG'GnC_Cy
Cuts at: 185 245 624 834 1042 1177 1578 185
Size: 60 379 210 208 135 401 3046
Fragments arranged by size:
3046 401 379 210 208 135 60
EcoRI G'AATT_C
Cuts at: 1443 1443
Size: 4439
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4439
FseI GG_CCGG'CC
Cuts at: 895 895
Size: 4439
FspI TGC'GCA
Cuts at: 2220 2220
Size: 4439
HaeII r_GCGC'y
Cuts at: 158 255 507 796 1298 1873 2121 3351 4122
Size: 97 252 289 502 575 248 1230 771
Cuts at: 4122 158
Size: 475
Fragments arranged by size:
1230 771 575 502 475 289 252 248
97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 328 360 361 393 969 1001 2281
Size: 32 1 32 1 32 576 32 1280
Cuts at: 2281 2311 2313 2343 295
Size: 30 2 30 2391
Fragments arranged by size:
2391 1280 576 32 32 32 32 30
30 2 1 1
HincII GTy'rAC
Cuts at: 1478 1478
Size: 4439
KasI G'GCGC_C
Cuts at: 154 792 1294 1869 2117 154
Size: 638 502 575 248 2476
Fragments arranged by size:
2476 638 575 502 248
KpnI G_GTAC'C
Cuts at: 1459 1459
Size: 4439
MluI A'CGCG_T
Cuts at: 3872 3872
Size: 4439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3198 3382 3198
Size: 184 4255
MscI TGG'CCA
Cuts at: 637 658 2200 637
Size: 21 1542 2876
Fragments arranged by size:
2876 1542 21
MslI CAynn'nnrTG
Cuts at: 592 967 2428 2555 592
Size: 375 1461 127 2476
Fragments arranged by size:
2476 1461 375 127
MspA1I CmG'CkG
Cuts at: 172 191 210 241 1219 1227 1550 1864 2224
Size: 19 19 31 978 8 323 314 360
Cuts at: 2224 3008 3253 172
Size: 784 245 1358
Fragments arranged by size:
1358 978 784 360 323 314 245 31
19 19 8
NaeI GCC'GGC
Cuts at: 153 893 2620 153
Size: 740 1727 1972
Fragments arranged by size:
1972 1727 740
NarI GG'CG_CC
Cuts at: 155 793 1295 1870 2118 155
Size: 638 502 575 248 2476
Fragments arranged by size:
2476 638 575 502 248
NcoI C'CATG_G
Cuts at: 593 593
Size: 4439
NgoMIV G'CCGG_C
Cuts at: 151 891 2618 151
Size: 740 1727 1972
Fragments arranged by size:
1972 1727 740
NspI r_CATG'y
Cuts at: 1492 1992 3595 1492
Size: 500 1603 2336
Fragments arranged by size:
2336 1603 500
PasI CC'CwG_GG
Cuts at: 939 939
Size: 4439
PciI A'CATG_T
Cuts at: 3591 3591
Size: 4439
PflMI CCAn_nnn'nTGG
Cuts at: 471 471
Size: 4439
PfoI T'CCnGG_A
Cuts at: 551 1010 1187 1244 551
Size: 459 177 57 3746
Fragments arranged by size:
3746 459 177 57
PmeI GTTT'AAAC
Cuts at: 1438 1438
Size: 4439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 4407
PpuMI rG'GwC_Cy
Cuts at: 624 624
Size: 4439
PshAI GACnn'nnGTC
Cuts at: 547 547
Size: 4439
PsiI TTA'TAA
Cuts at: 715 715
Size: 4439
PspOMI G'GGCC_C
Cuts at: 178 185 245 1042 1176 178
Size: 7 60 797 134 3441
Fragments arranged by size:
3441 797 134 60 7
PstI C_TGCA'G
Cuts at: 684 1486 2171 684
Size: 802 685 2952
Fragments arranged by size:
2952 802 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4439
PvuII CAG'CTG
Cuts at: 1219 1227 1864 2224 1219
Size: 8 637 360 3434
Fragments arranged by size:
3434 637 360 8
RsrII CG'GwC_CG
Cuts at: 2634 2634
Size: 4439
SacI G_AGCT'C
Cuts at: 494 1453 494
Size: 959 3480
SacII CC_GC'GG
Cuts at: 173 192 242 173
Size: 19 50 4370
Fragments arranged by size:
4370 50 19
SalI G'TCGA_C
Cuts at: 1476 1476
Size: 4439
SanDI GG'GwC_CC
Cuts at: 624 624
Size: 4439
SapI GCTCTTCn'nnn_
Cuts at: 482 551 2462 2672 482
Size: 69 1911 210 2249
Fragments arranged by size:
2249 1911 210 69
SbfI CC_TGCA'GG
Cuts at: 1486 1486
Size: 4439
ScaI AGT'ACT
Cuts at: 3956 3956
Size: 4439
SfcI C'TryA_G
Cuts at: 33 680 1482 2167 3135 3326 33
Size: 647 802 685 968 191 1146
Fragments arranged by size:
1146 968 802 685 647 191
SfoI GGC'GCC
Cuts at: 156 794 1296 1871 2119 156
Size: 638 502 575 248 2476
Fragments arranged by size:
2476 638 575 502 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
SmaI CCC'GGG
Cuts at: 183 1175 1461 183
Size: 992 286 3161
Fragments arranged by size:
3161 992 286
SmlI C'TyrA_G
Cuts at: 1594 2509 3223 3485 1594
Size: 915 714 262 2548
Fragments arranged by size:
2548 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4439
SphI G_CATG'C
Cuts at: 1492 1992 1492
Size: 500 3939
SrfI GCCC'GGGC
Cuts at: 1175 1175
Size: 4439
StyI C'CwwG_G
Cuts at: 593 1130 1160 1573 1927 593
Size: 537 30 413 354 3105
Fragments arranged by size:
3105 537 413 354 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 858 916 2022 2155 2614 2823 858
Size: 58 1106 133 459 209 2474
Fragments arranged by size:
2474 1106 459 209 133 58
TatI w'GTAC_w
Cuts at: 3954 3954
Size: 4439
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3956 3988 3956
Size: 32 4407
Tth111I GACn'n_nGTC
Cuts at: 2236 2236
Size: 4439
XbaI T'CTAG_A
Cuts at: 1470 1470
Size: 4439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 531 534 1934 531
Size: 3 1400 3036
Fragments arranged by size:
3036 1400 3
XmaI C'CCGG_G
Cuts at: 181 1173 1459 181
Size: 992 286 3161
Fragments arranged by size:
3161 992 286
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AloI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI
BsmI BspEI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PspXI PsrI
SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI
XmnI ZraI