Restriction map for
pF1KB9508
(Circular) (Six-base) MAPSORT of: pF1KB9508.seq Check: 3344 from: 1 to: 4142
pF1KB9508 4142 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 540 961 540
Size: 421 3721
AccI GT'mk_AC
Cuts at: 329 503 1180 329
Size: 174 677 3291
Fragments arranged by size:
3291 677 174
Acc65I G'GTAC_C
Cuts at: 1158 1158
Size: 4142
AclI AA'CG_TT
Cuts at: 3982 3982
Size: 4142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 392 447 1967 2399 3146 3414 151
Size: 241 55 1520 432 747 268 879
Fragments arranged by size:
1520 879 747 432 268 241 55
AflIII A'CryG_T
Cuts at: 1494 2598 3575 1494
Size: 1104 977 2061
Fragments arranged by size:
2061 1104 977
AgeI A'CCGG_T
Cuts at: 2530 2530
Size: 4142
AleI CACnn'nnGTG
Cuts at: 439 439
Size: 4142
AlwNI CAG_nnn'CTG
Cuts at: 295 1447 3014 295
Size: 1152 1567 1423
Fragments arranged by size:
1567 1423 1152
ApaI G_GGCC'C
Cuts at: 353 353
Size: 4142
ApaLI G'TGCA_C
Cuts at: 238 2912 238
Size: 2674 1468
ApoI r'AATT_y
Cuts at: 1146 3495 3751 1146
Size: 2349 256 1537
Fragments arranged by size:
2349 1537 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
AvaI C'yCGr_G
Cuts at: 1059 1162 1059
Size: 103 4039
BamHI G'GATC_C
Cuts at: 1167 1167
Size: 4142
BanI G'GyrC_C
Cuts at: 673 1158 1572 1820 1855 673
Size: 485 414 248 35 2960
Fragments arranged by size:
2960 485 414 248 35
BanII G_rGCy'C
Cuts at: 353 805 1156 2186 353
Size: 452 351 1030 2309
Fragments arranged by size:
2309 1030 452 351
BbeI G_GCGC'C
Cuts at: 1576 1824 1576
Size: 248 3894
BbsI GAAGACnn'nnnn_
Cuts at: 156 156
Size: 4142
BciVI GTATCCnnnnn_n'
Cuts at: 2040 2801 4142 2040
Size: 761 1341 2040
Fragments arranged by size:
2040 1341 761
BclI T'GATC_A
Cuts at: 1662 1662
Size: 4142
BglII A'GATC_T
Cuts at: 1657 3694 1657
Size: 2037 2105
BlpI GC'TnA_GC
Cuts at: 1254 1254
Size: 4142
Bme1580I G_kGCm'C
Cuts at: 242 353 847 1767 1860 2916 242
Size: 111 494 920 93 1056 1468
Fragments arranged by size:
1468 1056 920 494 111 93
BmgBI CAC'GTC
Cuts at: 903 903
Size: 4142
BmrI ACTGGGnnnn_n'
Cuts at: 412 466 1354 1435 1770 3914 412
Size: 54 888 81 335 2144 640
Fragments arranged by size:
2144 888 640 335 81 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 4142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 235
Size: 4142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 2197 2689 2987 1318
Size: 879 492 298 2473
Fragments arranged by size:
2473 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 114 3846 114
Size: 3732 410
BsaAI yAC'GTr
Cuts at: 777 1495 777
Size: 718 3424
BsaBI GATnn'nnATC
Cuts at: 6 1656 3693 6
Size: 1650 2037 455
Fragments arranged by size:
2037 1650 455
BsaHI Gr'CG_yC
Cuts at: 1573 1821 2523 4019 1573
Size: 248 702 1496 1696
Fragments arranged by size:
1696 1496 702 248
BsaWI w'CCGG_w
Cuts at: 633 1331 1852 2530 2804 2951 3362 633
Size: 698 521 678 274 147 411 1413
Fragments arranged by size:
1413 698 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 150 150
Size: 4142
BseYI C'CCAG_C
Cuts at: 183 369 691 1567 2325 2902 3398 183
Size: 186 322 876 758 577 496 927
Fragments arranged by size:
927 876 758 577 496 322 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 383
Size: 4142
BsiEI CG_ry'CG
Cuts at: 104 654 1684 1730 2938 104
Size: 550 1030 46 1208 1308
Fragments arranged by size:
1308 1208 1030 550 46
BsiHKAI G_wGCw'C
Cuts at: 242 436 1156 1934 2124 2916 242
Size: 194 720 778 190 792 1468
Fragments arranged by size:
1468 792 778 720 194 190
BsmI GAATG_Cn'
Cuts at: 1076 1076
Size: 4142
Bsp1286I G_dGCh'C
Cuts at: 242 353 436 805 847 1156 1767 1860 1934
Size: 111 83 369 42 309 611 93 74
Cuts at: 1934 2124 2186 2916 242
Size: 190 62 730 1468
Fragments arranged by size:
1468 730 611 369 309 190 111 93
83 74 62 42
BspEI T'CCGG_A
Cuts at: 633 3362 633
Size: 2729 1413
BspHI T'CATG_A
Cuts at: 3318 3318
Size: 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 540 961 1192 1406 1708 2089 540
Size: 421 231 214 302 381 2593
Fragments arranged by size:
2593 421 381 302 231 214
BsrBI CCG'CTC
Cuts at: 50 2434 2488 3972 4137 50
Size: 2384 54 1484 165 55
Fragments arranged by size:
2384 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2054 2054
Size: 4142
BsrFI r'CCGG_y
Cuts at: 559 977 1085 2140 2321 2530 559
Size: 418 108 1055 181 209 2171
Fragments arranged by size:
2171 1055 418 209 181 108
BssHII G'CGCG_C
Cuts at: 709 709
Size: 4142
BssSI C'ACGA_G
Cuts at: 697 784 2413 2771 697
Size: 87 1629 358 2068
Fragments arranged by size:
2068 1629 358 87
BstAPI GCAn_nnn'nTGC
Cuts at: 1519 1519
Size: 4142
BstBI TT'CG_AA
Cuts at: 2503 2503
Size: 4142
BstEII G'GTnAC_C
Cuts at: 555 756 948 555
Size: 201 192 3749
Fragments arranged by size:
3749 201 192
BstXI CCAn_nnnn'nTGG
Cuts at: 699 945 699
Size: 246 3896
BstYI r'GATC_y
Cuts at: 1 1167 1634 1657 1992 2238 3250 3336 3348
Size: 1166 467 23 335 246 1012 86 12
Cuts at: 3348 3694 1
Size: 346 449
Fragments arranged by size:
1166 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 296 296
Size: 4142
BtgI C'CryG_G
Cuts at: 107 1034 1127 107
Size: 927 93 3122
Fragments arranged by size:
3122 927 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 784 918 2092 2273 2436 784
Size: 134 1174 181 163 2490
Fragments arranged by size:
2490 1174 181 163 134
BtsI GCAGTG_nn'
Cuts at: 341 1385 341
Size: 1044 3098
DraI TTT'AAA
Cuts at: 1141 1141
Size: 4142
DrdI GACnn_nn'nnGTC
Cuts at: 658 1848 2706 658
Size: 1190 858 2094
Fragments arranged by size:
2094 1190 858
EaeI y'GGCC_r
Cuts at: 640 701 875 929 979 990 1130 1727 1901
Size: 61 174 54 50 11 140 597 174
Cuts at: 1901 2292 2319 3664 640
Size: 391 27 1345 1118
Fragments arranged by size:
1345 1118 597 391 174 174 140 61
54 50 27 11
EagI C'GGCC_G
Cuts at: 1727 1727
Size: 4142
EarI CTCTTCn'nnn_
Cuts at: 127 523 2165 2375 127
Size: 396 1642 210 1894
Fragments arranged by size:
1894 1642 396 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2350 2660 2806 3952 2350
Size: 310 146 1146 2540
Fragments arranged by size:
2540 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1154 1154
Size: 4142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 235 392 447 1967 2399 3146 3414 151
Size: 84 157 55 1520 432 747 268 879
Fragments arranged by size:
1520 879 747 432 268 157 84 55
EcoO109I rG'GnC_Cy
Cuts at: 292 449 646 1014 1281 292
Size: 157 197 368 267 3153
Fragments arranged by size:
3153 368 267 197 157
EcoRI G'AATT_C
Cuts at: 1146 1146
Size: 4142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 4142
FspI TGC'GCA
Cuts at: 1923 1923
Size: 4142
HaeII r_GCGC'y
Cuts at: 1576 1824 2846 3825 1576
Size: 248 1022 979 1893
Fragments arranged by size:
1893 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 549 581 1984 2014 2016 2046 111
Size: 32 406 32 1403 30 2 30 2207
Fragments arranged by size:
2207 1403 406 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1181 1181
Size: 4142
KasI G'GCGC_C
Cuts at: 1572 1820 1572
Size: 248 3894
KpnI G_GTAC'C
Cuts at: 1162 1162
Size: 4142
MluI A'CGCG_T
Cuts at: 3575 3575
Size: 4142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 878 2813 2997 878
Size: 1935 184 2023
Fragments arranged by size:
2023 1935 184
MscI TGG'CCA
Cuts at: 1132 1903 1132
Size: 771 3371
MslI CAynn'nnrTG
Cuts at: 439 769 2131 2258 439
Size: 330 1362 127 2323
Fragments arranged by size:
2323 1362 330 127
MspA1I CmG'CkG
Cuts at: 1253 1567 1927 2940 3185 1253
Size: 314 360 1013 245 2210
Fragments arranged by size:
2210 1013 360 314 245
NaeI GCC'GGC
Cuts at: 979 2323 979
Size: 1344 2798
NarI GG'CG_CC
Cuts at: 1573 1821 1573
Size: 248 3894
NcoI C'CATG_G
Cuts at: 107 1034 107
Size: 927 3215
NgoMIV G'CCGG_C
Cuts at: 977 2321 977
Size: 1344 2798
NspI r_CATG'y
Cuts at: 1195 1695 2602 1195
Size: 500 907 2735
Fragments arranged by size:
2735 907 500
PasI CC'CwG_GG
Cuts at: 246 289 612 1007 246
Size: 43 323 395 3381
Fragments arranged by size:
3381 395 323 43
PciI A'CATG_T
Cuts at: 2598 2598
Size: 4142
PflMI CCAn_nnn'nTGG
Cuts at: 988 988
Size: 4142
PmeI GTTT'AAAC
Cuts at: 1141 1141
Size: 4142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 4110
PpuMI rG'GwC_Cy
Cuts at: 646 1014 646
Size: 368 3774
PspOMI G'GGCC_C
Cuts at: 349 349
Size: 4142
PstI C_TGCA'G
Cuts at: 1071 1189 1874 1071
Size: 118 685 3339
Fragments arranged by size:
3339 685 118
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4142
PvuII CAG'CTG
Cuts at: 1567 1927 1567
Size: 360 3782
RsrII CG'GwC_CG
Cuts at: 2337 2337
Size: 4142
SacI G_AGCT'C
Cuts at: 1156 1156
Size: 4142
SalI G'TCGA_C
Cuts at: 1179 1179
Size: 4142
SapI GCTCTTCn'nnn_
Cuts at: 2165 2375 2165
Size: 210 3932
SbfI CC_TGCA'GG
Cuts at: 1189 1189
Size: 4142
ScaI AGT'ACT
Cuts at: 3659 3659
Size: 4142
SfcI C'TryA_G
Cuts at: 33 1067 1185 1870 2863 3054 33
Size: 1034 118 685 993 191 1121
Fragments arranged by size:
1121 1034 993 685 191 118
SfoI GGC'GCC
Cuts at: 1574 1822 1574
Size: 248 3894
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
SmaI CCC'GGG
Cuts at: 1164 1164
Size: 4142
SmlI C'TyrA_G
Cuts at: 1297 2212 2704 2966 1297
Size: 915 492 262 2473
Fragments arranged by size:
2473 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4142
SphI G_CATG'C
Cuts at: 1195 1695 1195
Size: 500 3642
StyI C'CwwG_G
Cuts at: 107 1034 1276 1630 107
Size: 927 242 354 2619
Fragments arranged by size:
2619 927 354 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 259 1725 1858 2317 2526 259
Size: 1466 133 459 209 1875
Fragments arranged by size:
1875 1466 459 209 133
TatI w'GTAC_w
Cuts at: 3657 3657
Size: 4142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 568 600 3659 3691 568
Size: 32 3059 32 1019
Fragments arranged by size:
3059 1019 32 32
Tth111I GACn'n_nGTC
Cuts at: 1939 1939
Size: 4142
XbaI T'CTAG_A
Cuts at: 1173 1173
Size: 4142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1637 1637
Size: 4142
XmaI C'CCGG_G
Cuts at: 1162 1162
Size: 4142
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI
BsiWI BsmBI BsrGI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI