Restriction map for
pF1KB9510
(Circular) (Six-base) MAPSORT of: pF1KB9510.seq Check: 4525 from: 1 to: 4229
pF1KB9510 4229 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1267 1267
Size: 4229
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 2054 2486 3233 3501 218
Size: 1836 432 747 268 946
Fragments arranged by size:
1836 946 747 432 268
AflII C'TTAA_G
Cuts at: 913 913
Size: 4229
AflIII A'CryG_T
Cuts at: 1581 2685 3662 1581
Size: 1104 977 2148
Fragments arranged by size:
2148 1104 977
AgeI A'CCGG_T
Cuts at: 2617 2617
Size: 4229
AleI CACnn'nnGTG
Cuts at: 216 216
Size: 4229
AlwNI CAG_nnn'CTG
Cuts at: 511 940 1534 3101 511
Size: 429 594 1567 1639
Fragments arranged by size:
1639 1567 594 429
ApaLI G'TGCA_C
Cuts at: 2999 2999
Size: 4229
ApoI r'AATT_y
Cuts at: 1233 3582 3838 1233
Size: 2349 256 1624
Fragments arranged by size:
2349 1624 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 1249 1249
Size: 4229
AvrII C'CTAG_G
Cuts at: 241 241
Size: 4229
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4229
BanI G'GyrC_C
Cuts at: 1147 1245 1659 1907 1942 1147
Size: 98 414 248 35 3434
Fragments arranged by size:
3434 414 248 98 35
BanII G_rGCy'C
Cuts at: 1243 2273 1243
Size: 1030 3199
BbeI G_GCGC'C
Cuts at: 1663 1911 1663
Size: 248 3981
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2888 4229 2127
Size: 761 1341 2127
Fragments arranged by size:
2127 1341 761
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4229
BglII A'GATC_T
Cuts at: 1744 3781 1744
Size: 2037 2192
BlpI GC'TnA_GC
Cuts at: 175 449 1341 175
Size: 274 892 3063
Fragments arranged by size:
3063 892 274
Bme1580I G_kGCm'C
Cuts at: 1854 1947 3003 1854
Size: 93 1056 3080
Fragments arranged by size:
3080 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1441 1522 1857 4001 1441
Size: 81 335 2144 1669
Fragments arranged by size:
2144 1669 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 1191 773
Size: 418 3811
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2284 2776 3074 1405
Size: 879 492 298 2560
Fragments arranged by size:
2560 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3933 3933
Size: 4229
BsaAI yAC'GTr
Cuts at: 218 867 1582 218
Size: 649 715 2865
Fragments arranged by size:
2865 715 649
BsaBI GATnn'nnATC
Cuts at: 6 1743 3780 6
Size: 1737 2037 455
Fragments arranged by size:
2037 1737 455
BsaHI Gr'CG_yC
Cuts at: 1660 1908 2610 4106 1660
Size: 248 702 1496 1783
Fragments arranged by size:
1783 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1418 1939 2617 2891 3038 3449 1418
Size: 521 678 274 147 411 2198
Fragments arranged by size:
2198 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 343 1654 2412 2989 3485 343
Size: 1311 758 577 496 1087
Fragments arranged by size:
1311 1087 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3025 104
Size: 1667 46 1208 1308
Fragments arranged by size:
1667 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1243 2021 2211 3003 1243
Size: 778 190 792 2469
Fragments arranged by size:
2469 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1243 1854 1947 2021 2211 2273 3003 1243
Size: 611 93 74 190 62 730 2469
Fragments arranged by size:
2469 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3449 3449
Size: 4229
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 510 1279 1493 1795 2176 510
Size: 769 214 302 381 2563
Fragments arranged by size:
2563 769 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2521 2575 4059 4224 50
Size: 2471 54 1484 165 55
Fragments arranged by size:
2471 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 890 2141 890
Size: 1251 2978
BsrFI r'CCGG_y
Cuts at: 2227 2408 2617 2227
Size: 181 209 3839
Fragments arranged by size:
3839 209 181
BssSI C'ACGA_G
Cuts at: 2500 2858 2500
Size: 358 3871
BstAPI GCAn_nnn'nTGC
Cuts at: 1078 1606 1078
Size: 528 3701
BstBI TT'CG_AA
Cuts at: 2590 2590
Size: 4229
BstXI CCAn_nnnn'nTGG
Cuts at: 287 287
Size: 4229
BstYI r'GATC_y
Cuts at: 1 302 1254 1721 1744 2079 2325 3337 3423
Size: 301 952 467 23 335 246 1012 86
Cuts at: 3423 3435 3781 1
Size: 12 346 449
Fragments arranged by size:
1012 952 467 449 346 335 301 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 560 560
Size: 4229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2179 2360 2523 2179
Size: 181 163 3885
Fragments arranged by size:
3885 181 163
BtsI GCAGTG_nn'
Cuts at: 485 1472 485
Size: 987 3242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 807 842 807
Size: 35 4194
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 1935 2793 1935
Size: 858 3371
EaeI y'GGCC_r
Cuts at: 1814 1988 2379 2406 3751 1814
Size: 174 391 27 1345 2292
Fragments arranged by size:
2292 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4229
EarI CTCTTCn'nnn_
Cuts at: 659 2252 2462 659
Size: 1593 210 2426
Fragments arranged by size:
2426 1593 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2437 2747 2893 4039 2437
Size: 310 146 1146 2627
Fragments arranged by size:
2627 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1241 1241
Size: 4229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 773 1191 2054 2486 3233 3501 218
Size: 555 418 863 432 747 268 946
Fragments arranged by size:
946 863 747 555 432 418 268
EcoNI CCTnn'n_nnAGG
Cuts at: 190 190
Size: 4229
EcoO109I rG'GnC_Cy
Cuts at: 465 1368 465
Size: 903 3326
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 4229
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4229
HaeII r_GCGC'y
Cuts at: 1663 1911 2933 3912 1663
Size: 248 1022 979 1980
Fragments arranged by size:
1980 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2071 2101 2103 2133 2071
Size: 30 2 30 4167
Fragments arranged by size:
4167 30 30 2
HincII GTy'rAC
Cuts at: 113 587 1268 113
Size: 474 681 3074
Fragments arranged by size:
3074 681 474
HindIII A'AGCT_T
Cuts at: 1197 1197
Size: 4229
KasI G'GCGC_C
Cuts at: 1659 1907 1659
Size: 248 3981
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2900 3084 2900
Size: 184 4045
MscI TGG'CCA
Cuts at: 1990 1990
Size: 4229
MslI CAynn'nnrTG
Cuts at: 216 869 971 2218 2345 216
Size: 653 102 1247 127 2100
Fragments arranged by size:
2100 1247 653 127 102
MspA1I CmG'CkG
Cuts at: 118 453 511 1340 1654 2014 3027 3272 118
Size: 335 58 829 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 829 360 335 314 245 58
NaeI GCC'GGC
Cuts at: 2410 2410
Size: 4229
NarI GG'CG_CC
Cuts at: 1660 1908 1660
Size: 248 3981
NgoMIV G'CCGG_C
Cuts at: 2408 2408
Size: 4229
NspI r_CATG'y
Cuts at: 1282 1782 2689 1282
Size: 500 907 2822
Fragments arranged by size:
2822 907 500
PciI A'CATG_T
Cuts at: 2685 2685
Size: 4229
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PmlI CAC'GTG
Cuts at: 218 218
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 4197
PpuMI rG'GwC_Cy
Cuts at: 465 465
Size: 4229
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1082 1114 1082
Size: 32 4197
PstI C_TGCA'G
Cuts at: 194 507 1131 1276 1961 194
Size: 313 624 145 685 2462
Fragments arranged by size:
2462 685 624 313 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 118 453 511 1654 2014 118
Size: 335 58 1143 360 2333
Fragments arranged by size:
2333 1143 360 335 58
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 1243 1243
Size: 4229
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 2252 2462 2252
Size: 210 4019
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 1125 3746 1125
Size: 2621 1608
SexAI A'CCwGG_T
Cuts at: 152 152
Size: 4229
SfcI C'TryA_G
Cuts at: 33 190 503 1127 1272 1957 2950 3141 33
Size: 157 313 624 145 685 993 191 1121
Fragments arranged by size:
1121 993 685 624 313 191 157 145
SfoI GGC'GCC
Cuts at: 1661 1909 1661
Size: 248 3981
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4229
SmlI C'TyrA_G
Cuts at: 913 1384 2299 2791 3053 913
Size: 471 915 492 262 2089
Fragments arranged by size:
2089 915 492 471 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3729
StyI C'CwwG_G
Cuts at: 241 590 1363 1717 241
Size: 349 773 354 2753
Fragments arranged by size:
2753 773 354 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 228 357 1812 1945 2404 2613 228
Size: 129 1455 133 459 209 1844
Fragments arranged by size:
1844 1455 459 209 133 129
TatI w'GTAC_w
Cuts at: 470 1123 3744 470
Size: 653 2621 955
Fragments arranged by size:
2621 955 653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4197
Tth111I GACn'n_nGTC
Cuts at: 1042 2026 1042
Size: 984 3245
XbaI T'CTAG_A
Cuts at: 600 1260 600
Size: 660 3569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 378 1724 378
Size: 1346 2883
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4229
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PmlI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I BtgI ClaI DraIII EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PshAI PsiI PspOMI
PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI