Restriction map for
pF1KB9519
(Circular) (Six-base) MAPSORT of: pF1KB9519.seq Check: 8287 from: 1 to: 4232
pF1KB9519 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 716 1270 716
Size: 554 3678
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 2057 2489 3236 3504 363
Size: 1694 432 747 268 1091
Fragments arranged by size:
1694 1091 747 432 268
AflIII A'CryG_T
Cuts at: 1584 2688 3665 1584
Size: 1104 977 2151
Fragments arranged by size:
2151 1104 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 408 408
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 1537 3104 1537
Size: 1567 2665
ApaI G_GGCC'C
Cuts at: 866 866
Size: 4232
ApaLI G'TGCA_C
Cuts at: 383 3002 383
Size: 2619 1613
ApoI r'AATT_y
Cuts at: 1236 3585 3841 1236
Size: 2349 256 1627
Fragments arranged by size:
2349 1627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 811 1252 811
Size: 441 3791
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 595 774 1192 1248 1662 1910 1945 595
Size: 179 418 56 414 248 35 2882
Fragments arranged by size:
2882 418 414 248 179 56 35
BanII G_rGCy'C
Cuts at: 866 1246 2276 866
Size: 380 1030 2822
Fragments arranged by size:
2822 1030 380
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 966 1000 966
Size: 34 4198
BciVI GTATCCnnnnn_n'
Cuts at: 898 2130 2891 4232 898
Size: 1232 761 1341 898
Fragments arranged by size:
1341 1232 898 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BglI GCCn_nnn'nGGC
Cuts at: 412 412
Size: 4232
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 264 387 598 866 1061 1186 1197 1857 1950
Size: 123 211 268 195 125 11 660 93
Cuts at: 1950 3006 264
Size: 1056 1490
Fragments arranged by size:
1490 1056 660 268 211 195 125 123
93 11
BmgBI CAC'GTC
Cuts at: 591 591
Size: 4232
BmrI ACTGGGnnnn_n'
Cuts at: 934 1444 1525 1860 4004 934
Size: 510 81 335 2144 1162
Fragments arranged by size:
2144 1162 510 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 164 2599 164
Size: 2435 1797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1132 1408 2287 2779 3077 235
Size: 897 276 879 492 298 1390
Fragments arranged by size:
1390 897 879 492 298 276
BsaI GGTCTCn'nnnn_
Cuts at: 3936 3936
Size: 4232
BsaAI yAC'GTr
Cuts at: 1585 1585
Size: 4232
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 1663 1911 2613 4109 1663
Size: 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 248
BsaWI w'CCGG_w
Cuts at: 121 1421 1942 2620 2894 3041 3452 121
Size: 1300 521 678 274 147 411 901
Fragments arranged by size:
1300 901 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 640 610
Size: 30 4202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 631 721 988 148
Size: 483 90 267 3392
Fragments arranged by size:
3392 483 267 90
BseYI C'CCAG_C
Cuts at: 192 666 882 957 1178 1657 2415 2992 3488
Size: 474 216 75 221 479 758 577 496
Cuts at: 3488 192
Size: 936
Fragments arranged by size:
936 758 577 496 479 474 221 216
75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 787 250
Size: 537 3695
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 251 387 422 695 1246 2024 2214 3006 251
Size: 136 35 273 551 778 190 792 1477
Fragments arranged by size:
1477 792 778 551 273 190 136 35
Bsp1286I G_dGCh'C
Cuts at: 251 264 387 422 598 695 866 1061 1186
Size: 13 123 35 176 97 171 195 125
Cuts at: 1186 1197 1246 1857 1950 2024 2214 2276 3006
Size: 11 49 611 93 74 190 62 730
Cuts at: 3006 251
Size: 1477
Fragments arranged by size:
1477 730 611 195 190 176 171 125
123 97 93 74 62 49 35 13
11
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1282 1496 1798 2179 1282
Size: 214 302 381 3335
Fragments arranged by size:
3335 381 302 214
BsrBI CCG'CTC
Cuts at: 50 143 2524 2578 4062 4227 50
Size: 93 2381 54 1484 165 55
Fragments arranged by size:
2381 1484 165 93 55 54
BsrDI GCAATG_nn'
Cuts at: 2144 2144
Size: 4232
BsrFI r'CCGG_y
Cuts at: 820 847 914 2230 2411 2620 820
Size: 27 67 1316 181 209 2432
Fragments arranged by size:
2432 1316 209 181 67 27
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 774 1609 774
Size: 835 3397
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstEII G'GTnAC_C
Cuts at: 1004 1004
Size: 4232
BstYI r'GATC_y
Cuts at: 1 1257 1724 1747 2082 2328 3340 3426 3438
Size: 1256 467 23 335 246 1012 86 12
Cuts at: 3438 3784 1
Size: 346 449
Fragments arranged by size:
1256 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 131 1159 131
Size: 1028 3204
BtgI C'CryG_G
Cuts at: 107 360 566 804 858 107
Size: 253 206 238 54 3481
Fragments arranged by size:
3481 253 238 206 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 465 625 2182 2363 2526 465
Size: 160 1557 181 163 2171
Fragments arranged by size:
2171 1557 181 163 160
BtsI GCAGTG_nn'
Cuts at: 982 1076 1475 982
Size: 94 399 3739
Fragments arranged by size:
3739 399 94
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 110 305 413 476 563 822 855 1073 1817
Size: 195 108 63 87 259 33 218 744
Cuts at: 1817 1991 2382 2409 3754 110
Size: 174 391 27 1345 588
Fragments arranged by size:
1345 744 588 391 259 218 195 174
108 87 63 33 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 372 739 2255 2465 372
Size: 367 1516 210 2139
Fragments arranged by size:
2139 1516 367 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1244
Size: 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 2057 2489 3236 3504 363
Size: 1694 432 747 268 1091
Fragments arranged by size:
1694 1091 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 268 268
Size: 4232
EcoO109I rG'GnC_Cy
Cuts at: 1143 1371 1143
Size: 228 4004
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 149 1666 1914 2936 3915 149
Size: 1517 248 1022 979 466
Fragments arranged by size:
1517 1022 979 466 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2074 2104 2106 2136 2074
Size: 30 2 30 4170
Fragments arranged by size:
4170 30 30 2
HincII GTy'rAC
Cuts at: 1113 1271 1113
Size: 158 4074
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MfeI C'AATT_G
Cuts at: 1118 1118
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3087 2903
Size: 184 4048
MscI TGG'CCA
Cuts at: 112 478 565 1075 1993 112
Size: 366 87 510 918 2351
Fragments arranged by size:
2351 918 510 366 87
MslI CAynn'nnrTG
Cuts at: 541 679 2221 2348 541
Size: 138 1542 127 2425
Fragments arranged by size:
2425 1542 138 127
MspA1I CmG'CkG
Cuts at: 392 957 1343 1657 2017 3030 3275 392
Size: 565 386 314 360 1013 245 1349
Fragments arranged by size:
1349 1013 565 386 360 314 245
NaeI GCC'GGC
Cuts at: 849 916 2413 849
Size: 67 1497 2668
Fragments arranged by size:
2668 1497 67
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NcoI C'CATG_G
Cuts at: 107 566 804 107
Size: 459 238 3535
Fragments arranged by size:
3535 459 238
NgoMIV G'CCGG_C
Cuts at: 847 914 2411 847
Size: 67 1497 2668
Fragments arranged by size:
2668 1497 67
NspI r_CATG'y
Cuts at: 527 907 969 1285 1785 2692 527
Size: 380 62 316 500 907 2067
Fragments arranged by size:
2067 907 500 380 316 62
PciI A'CATG_T
Cuts at: 2688 2688
Size: 4232
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PpuMI rG'GwC_Cy
Cuts at: 1143 1143
Size: 4232
PspOMI G'GGCC_C
Cuts at: 862 862
Size: 4232
PstI C_TGCA'G
Cuts at: 1279 1964 1279
Size: 685 3547
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 392 957 1657 2017 392
Size: 565 700 360 2607
Fragments arranged by size:
2607 700 565 360
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 1246 1246
Size: 4232
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 372 2255 2465 372
Size: 1883 210 2139
Fragments arranged by size:
2139 1883 210
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SfcI C'TryA_G
Cuts at: 33 1275 1960 2953 3144 33
Size: 1242 685 993 191 1121
Fragments arranged by size:
1242 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 412 412
Size: 4232
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 813 1254 813
Size: 441 3791
SmlI C'TyrA_G
Cuts at: 250 1147 1387 2302 2794 3056 250
Size: 897 240 915 492 262 1426
Fragments arranged by size:
1426 915 897 492 262 240
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 907 1285 1785 907
Size: 378 500 3354
Fragments arranged by size:
3354 500 378
StyI C'CwwG_G
Cuts at: 107 566 804 1366 1720 107
Size: 459 238 562 354 2619
Fragments arranged by size:
2619 562 459 354 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1815 1948 2407 2616 1815
Size: 133 459 209 3431
Fragments arranged by size:
3431 459 209 133
TatI w'GTAC_w
Cuts at: 3747 3747
Size: 4232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 366 398 610 642 3749 3781 366
Size: 32 212 32 3107 32 817
Fragments arranged by size:
3107 817 212 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 555 937 2029 555
Size: 382 1092 2758
Fragments arranged by size:
2758 1092 382
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 663 1727 663
Size: 1064 3168
XmaI C'CCGG_G
Cuts at: 811 1252 811
Size: 441 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI BpmI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI