Restriction map for
pF1KB9520
(Circular) (Six-base) MAPSORT of: pF1KB9520.seq Check: 1958 from: 1 to: 4448
pF1KB9520 4448 bp
With 182 enzymes: SgfI *
November 2, 2010 17:11 ..
AccI GT'mk_AC
Cuts at: 1486 1486
Size: 4448
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 2705 3452 3720 2273
Size: 432 747 268 3001
Fragments arranged by size:
3001 747 432 268
AfeI AGC'GCT
Cuts at: 1147 1147
Size: 4448
AflIII A'CryG_T
Cuts at: 1800 2904 3881 1800
Size: 1104 977 2367
Fragments arranged by size:
2367 1104 977
AgeI A'CCGG_T
Cuts at: 2836 2836
Size: 4448
AlwNI CAG_nnn'CTG
Cuts at: 1753 3320 1753
Size: 1567 2881
ApaI G_GGCC'C
Cuts at: 540 1224 540
Size: 684 3764
ApaLI G'TGCA_C
Cuts at: 459 3218 459
Size: 2759 1689
ApoI r'AATT_y
Cuts at: 1452 3801 4057 1452
Size: 2349 256 1843
Fragments arranged by size:
2349 1843 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 294 518 658 815 832 844 979 1038 1160
Size: 224 140 157 17 12 135 59 122
Cuts at: 1160 1468 294
Size: 308 3274
Fragments arranged by size:
3274 308 224 157 140 135 122 59
17 12
BamHI G'GATC_C
Cuts at: 1473 1473
Size: 4448
BanI G'GyrC_C
Cuts at: 358 1464 1878 2126 2161 358
Size: 1106 414 248 35 2645
Fragments arranged by size:
2645 1106 414 248 35
BanII G_rGCy'C
Cuts at: 540 816 839 1078 1214 1224 1462 2492 540
Size: 276 23 239 136 10 238 1030 2496
Fragments arranged by size:
2496 1030 276 239 238 136 23 10
BbeI G_GCGC'C
Cuts at: 362 1882 2130 362
Size: 1520 248 2680
Fragments arranged by size:
2680 1520 248
BbvCI CC'TCA_GC
Cuts at: 742 763 959 742
Size: 21 196 4231
Fragments arranged by size:
4231 196 21
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3107 4448 2346
Size: 761 1341 2346
Fragments arranged by size:
2346 1341 761
BclI T'GATC_A
Cuts at: 1241 1968 1241
Size: 727 3721
BglI GCCn_nnn'nGGC
Cuts at: 129 286 970 1321 129
Size: 157 684 351 3256
Fragments arranged by size:
3256 684 351 157
BglII A'GATC_T
Cuts at: 1963 4000 1963
Size: 2037 2411
BlpI GC'TnA_GC
Cuts at: 1560 1560
Size: 4448
Bme1580I G_kGCm'C
Cuts at: 463 540 905 1048 1224 2073 2166 3222 463
Size: 77 365 143 176 849 93 1056 1689
Fragments arranged by size:
1689 1056 849 365 176 143 93 77
BmrI ACTGGGnnnn_n'
Cuts at: 1660 1741 2076 4220 1660
Size: 81 335 2144 1888
Fragments arranged by size:
2144 1888 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1005 1126 1447 742
Size: 263 121 321 3743
Fragments arranged by size:
3743 321 263 121
Bpu10I CC'TnA_GC
Cuts at: 622 742 763 959 622
Size: 120 21 196 4111
Fragments arranged by size:
4111 196 120 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1624 2503 2995 3293 1051
Size: 573 879 492 298 2206
Fragments arranged by size:
2206 879 573 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 531 4152 531
Size: 3621 827
BsaAI yAC'GTr
Cuts at: 390 1801 390
Size: 1411 3037
BsaBI GATnn'nnATC
Cuts at: 6 1243 1962 3999 6
Size: 1237 719 2037 455
Fragments arranged by size:
2037 1237 719 455
BsaHI Gr'CG_yC
Cuts at: 359 1090 1879 2127 2829 4325 359
Size: 731 789 248 702 1496 482
Fragments arranged by size:
1496 789 731 702 482 248
BsaWI w'CCGG_w
Cuts at: 1637 2158 2836 3110 3257 3668 1637
Size: 521 678 274 147 411 2417
Fragments arranged by size:
2417 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 622 652 1279 1309 622
Size: 30 627 30 3761
Fragments arranged by size:
3761 627 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 456 1026 456
Size: 570 3878
BseYI C'CCAG_C
Cuts at: 255 539 626 851 881 1142 1217 1873 2631
Size: 284 87 225 30 261 75 656 758
Cuts at: 2631 3208 3704 255
Size: 577 496 999
Fragments arranged by size:
999 758 656 577 496 284 261 225
87 75 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 4448
BsiEI CG_ry'CG
Cuts at: 104 417 501 950 1990 2036 3244 104
Size: 313 84 449 1040 46 1208 1308
Fragments arranged by size:
1308 1208 1040 449 313 84 46
BsiHKAI G_wGCw'C
Cuts at: 463 488 1433 1462 2240 2430 3222 463
Size: 25 945 29 778 190 792 1689
Fragments arranged by size:
1689 945 792 778 190 29 25
Bsp1286I G_dGCh'C
Cuts at: 463 488 540 816 839 905 1048 1078 1214
Size: 25 52 276 23 66 143 30 136
Cuts at: 1214 1224 1433 1462 2073 2166 2240 2430 2492
Size: 10 209 29 611 93 74 190 62
Cuts at: 2492 3222 463
Size: 730 1689
Fragments arranged by size:
1689 730 611 276 209 190 143 136
93 74 66 62 52 30 29 25
23 10
BspEI T'CCGG_A
Cuts at: 3668 3668
Size: 4448
BspHI T'CATG_A
Cuts at: 3624 3624
Size: 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 461 1498 1712 2014 2395 461
Size: 1037 214 302 381 2514
Fragments arranged by size:
2514 1037 381 302 214
BsrBI CCG'CTC
Cuts at: 50 523 2740 2794 4278 4443 50
Size: 473 2217 54 1484 165 55
Fragments arranged by size:
2217 1484 473 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2360 2360
Size: 4448
BsrFI r'CCGG_y
Cuts at: 826 2446 2627 2836 826
Size: 1620 181 209 2438
Fragments arranged by size:
2438 1620 209 181
BssHII G'CGCG_C
Cuts at: 114 116 184 114
Size: 2 68 4378
Fragments arranged by size:
4378 68 2
BssSI C'ACGA_G
Cuts at: 2719 3077 2719
Size: 358 4090
BstAPI GCAn_nnn'nTGC
Cuts at: 1825 1825
Size: 4448
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstEII G'GTnAC_C
Cuts at: 513 513
Size: 4448
BstXI CCAn_nnnn'nTGG
Cuts at: 255 1185 255
Size: 930 3518
BstYI r'GATC_y
Cuts at: 1 1473 1940 1963 2298 2544 3556 3642 3654
Size: 1472 467 23 335 246 1012 86 12
Cuts at: 3654 4000 1
Size: 346 449
Fragments arranged by size:
1472 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 209 248 1004 1260 107
Size: 102 39 756 256 3295
Fragments arranged by size:
3295 756 256 102 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2398 2579 2742 2398
Size: 181 163 4104
Fragments arranged by size:
4104 181 163
BtsI GCAGTG_nn'
Cuts at: 569 666 1266 1431 1691 569
Size: 97 600 165 260 3326
Fragments arranged by size:
3326 600 260 165 97
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 352 2154 3012 352
Size: 1802 858 1788
Fragments arranged by size:
1802 1788 858
EaeI y'GGCC_r
Cuts at: 218 245 287 414 947 1175 1187 1263 2033
Size: 27 42 127 533 228 12 76 770
Cuts at: 2033 2207 2598 2625 3970 218
Size: 174 391 27 1345 696
Fragments arranged by size:
1345 770 696 533 391 228 174 127
76 42 27 27 12
EagI C'GGCC_G
Cuts at: 414 947 2033 414
Size: 533 1086 2829
Fragments arranged by size:
2829 1086 533
EarI CTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1354 2656 2966 3112 4258 1354
Size: 1302 310 146 1146 1544
Fragments arranged by size:
1544 1302 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1460 1460
Size: 4448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1005 1126 1447 2273 2705 3452 3720 742
Size: 263 121 321 826 432 747 268 1470
Fragments arranged by size:
1470 826 747 432 321 268 263 121
EcoO109I rG'GnC_Cy
Cuts at: 420 1221 1587 420
Size: 801 366 3281
Fragments arranged by size:
3281 801 366
EcoRI G'AATT_C
Cuts at: 1452 1452
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 2229 2229
Size: 4448
HaeII r_GCGC'y
Cuts at: 154 235 362 959 1149 1882 2130 3152 4131
Size: 81 127 597 190 733 248 1022 979
Cuts at: 4131 154
Size: 471
Fragments arranged by size:
1022 979 733 597 471 248 190 127
81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 602 622 634 654 2290 2320 2322 2352 602
Size: 20 12 20 1636 30 2 30 2698
Fragments arranged by size:
2698 1636 30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 1329 1487 1329
Size: 158 4290
KasI G'GCGC_C
Cuts at: 358 1878 2126 358
Size: 1520 248 2680
Fragments arranged by size:
2680 1520 248
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 368 3119 3303 368
Size: 2751 184 1513
Fragments arranged by size:
2751 1513 184
MscI TGG'CCA
Cuts at: 247 1177 1265 2209 247
Size: 930 88 944 2486
Fragments arranged by size:
2486 944 930 88
MslI CAynn'nnrTG
Cuts at: 671 929 1120 2437 2564 671
Size: 258 191 1317 127 2555
Fragments arranged by size:
2555 1317 258 191 127
MspA1I CmG'CkG
Cuts at: 135 211 404 921 1559 1873 2233 3246 3491
Size: 76 193 517 638 314 360 1013 245
Cuts at: 3491 135
Size: 1092
Fragments arranged by size:
1092 1013 638 517 360 314 245 193
76
NaeI GCC'GGC
Cuts at: 828 2629 828
Size: 1801 2647
NarI GG'CG_CC
Cuts at: 359 1879 2127 359
Size: 1520 248 2680
Fragments arranged by size:
2680 1520 248
NcoI C'CATG_G
Cuts at: 107 1260 107
Size: 1153 3295
NgoMIV G'CCGG_C
Cuts at: 826 2627 826
Size: 1801 2647
NspI r_CATG'y
Cuts at: 1501 2001 2908 1501
Size: 500 907 3041
Fragments arranged by size:
3041 907 500
PciI A'CATG_T
Cuts at: 2904 2904
Size: 4448
PflMI CCAn_nnn'nTGG
Cuts at: 874 874
Size: 4448
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4416
PspOMI G'GGCC_C
Cuts at: 536 1220 536
Size: 684 3764
PspXI vC'TCGA_Gb
Cuts at: 294 1038 294
Size: 744 3704
PstI C_TGCA'G
Cuts at: 1495 2180 1495
Size: 685 3763
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 1873 2233 1873
Size: 360 4088
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 1462 1462
Size: 4448
SacII CC_GC'GG
Cuts at: 212 212
Size: 4448
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
SbfI CC_TGCA'GG
Cuts at: 1495 1495
Size: 4448
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SfcI C'TryA_G
Cuts at: 33 1491 2176 3169 3360 33
Size: 1458 685 993 191 1121
Fragments arranged by size:
1458 1121 993 685 191
SfoI GGC'GCC
Cuts at: 360 1880 2128 360
Size: 1520 248 2680
Fragments arranged by size:
2680 1520 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 1470 1470
Size: 4448
SmlI C'TyrA_G
Cuts at: 294 815 1038 1066 1603 2518 3010 3272 294
Size: 521 223 28 537 915 492 262 1470
Fragments arranged by size:
1470 915 537 521 492 262 223 28
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1501 2001 1501
Size: 500 3948
StyI C'CwwG_G
Cuts at: 107 1260 1582 1936 107
Size: 1153 322 354 2619
Fragments arranged by size:
2619 1153 354 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 322 501 576 928 1029 1156 2031 2164 2623
Size: 179 75 352 101 127 875 133 459
Cuts at: 2623 2832 322
Size: 209 1938
Fragments arranged by size:
1938 875 459 352 209 179 133 127
101 75
TatI w'GTAC_w
Cuts at: 3963 3963
Size: 4448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 4416
Tth111I GACn'n_nGTC
Cuts at: 2245 2245
Size: 4448
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1943 1943
Size: 4448
XhoI C'TCGA_G
Cuts at: 294 815 1038 294
Size: 521 223 3704
Fragments arranged by size:
3704 521 223
XmaI C'CCGG_G
Cuts at: 1468 1468
Size: 4448
XmnI GAAnn'nnTTC
Cuts at: 316 316
Size: 4448
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI