Restriction map for
pF1KB9521
(Circular) (Six-base) MAPSORT of: pF1KB9521.seq Check: 7560 from: 1 to: 4877
pF1KB9521 4877 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 427 427
Size: 4877
AccI GT'mk_AC
Cuts at: 1915 1915
Size: 4877
Acc65I G'GTAC_C
Cuts at: 808 828 1893 808
Size: 20 1065 3792
Fragments arranged by size:
3792 1065 20
AclI AA'CG_TT
Cuts at: 4717 4717
Size: 4877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 1541 2702 3134 3881 4149 639
Size: 902 1161 432 747 268 1367
Fragments arranged by size:
1367 1161 902 747 432 268
AfeI AGC'GCT
Cuts at: 592 1093 592
Size: 501 4376
AflIII A'CryG_T
Cuts at: 2229 3333 4310 2229
Size: 1104 977 2796
Fragments arranged by size:
2796 1104 977
AgeI A'CCGG_T
Cuts at: 3265 3265
Size: 4877
AleI CACnn'nnGTG
Cuts at: 961 961
Size: 4877
AlwNI CAG_nnn'CTG
Cuts at: 510 723 1059 2182 3749 510
Size: 213 336 1123 1567 1638
Fragments arranged by size:
1638 1567 1123 336 213
ApaI G_GGCC'C
Cuts at: 732 1452 1840 732
Size: 720 388 3769
Fragments arranged by size:
3769 720 388
ApaLI G'TGCA_C
Cuts at: 3647 3647
Size: 4877
ApoI r'AATT_y
Cuts at: 1881 4230 4486 1881
Size: 2349 256 2272
Fragments arranged by size:
2349 2272 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4877
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
AvaI C'yCGr_G
Cuts at: 113 854 1376 1453 1897 113
Size: 741 522 77 444 3093
Fragments arranged by size:
3093 741 522 444 77
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 354 387 354
Size: 33 4844
BamHI G'GATC_C
Cuts at: 1828 1902 1828
Size: 74 4803
BanI G'GyrC_C
Cuts at: 808 828 963 1008 1893 2307 2555 2590 808
Size: 20 135 45 885 414 248 35 3095
Fragments arranged by size:
3095 885 414 248 135 45 35 20
BanII G_rGCy'C
Cuts at: 182 732 747 1452 1840 1891 2921 182
Size: 550 15 705 388 51 1030 2138
Fragments arranged by size:
2138 1030 705 550 388 51 15
BbeI G_GCGC'C
Cuts at: 1012 2311 2559 1012
Size: 1299 248 3330
Fragments arranged by size:
3330 1299 248
BbsI GAAGACnn'nnnn_
Cuts at: 375 375
Size: 4877
BbvCI CC'TCA_GC
Cuts at: 214 487 214
Size: 273 4604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 316 350 366 400 1663 1697 316
Size: 34 16 34 1263 34 3496
Fragments arranged by size:
3496 1263 34 34 34 16
BciVI GTATCCnnnnn_n'
Cuts at: 2775 3536 4877 2775
Size: 761 1341 2775
Fragments arranged by size:
2775 1341 761
BclI T'GATC_A
Cuts at: 2397 2397
Size: 4877
BglI GCCn_nnn'nGGC
Cuts at: 1278 1278
Size: 4877
BglII A'GATC_T
Cuts at: 2392 4429 2392
Size: 2037 2840
BlpI GC'TnA_GC
Cuts at: 1989 1989
Size: 4877
Bme1580I G_kGCm'C
Cuts at: 280 305 732 968 1452 1676 1840 2502 2595
Size: 25 427 236 484 224 164 662 93
Cuts at: 2595 3651 280
Size: 1056 1506
Fragments arranged by size:
1506 1056 662 484 427 236 224 164
93 25
BmgBI CAC'GTC
Cuts at: 1107 1107
Size: 4877
BmrI ACTGGGnnnn_n'
Cuts at: 1708 2089 2170 2505 4649 1708
Size: 381 81 335 2144 1936
Fragments arranged by size:
2144 1936 381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 156 3244 156
Size: 3088 1789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 4877
Bpu10I CC'TnA_GC
Cuts at: 214 487 214
Size: 273 4604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 1321 2053 2932 3424 3722 979
Size: 342 732 879 492 298 2134
Fragments arranged by size:
2134 879 732 492 342 298
BsaI GGTCTCn'nnnn_
Cuts at: 4581 4581
Size: 4877
BsaAI yAC'GTr
Cuts at: 2230 2230
Size: 4877
BsaBI GATnn'nnATC
Cuts at: 6 2391 4428 6
Size: 2385 2037 455
Fragments arranged by size:
2385 2037 455
BsaHI Gr'CG_yC
Cuts at: 1009 1338 2308 2556 3258 4754 1009
Size: 329 970 248 702 1496 1132
Fragments arranged by size:
1496 1132 970 702 329 248
BsaWI w'CCGG_w
Cuts at: 2066 2587 3265 3539 3686 4097 2066
Size: 521 678 274 147 411 2846
Fragments arranged by size:
2846 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 1162 1286 1516 1630 1670 148
Size: 1014 124 230 114 40 3355
Fragments arranged by size:
3355 1014 230 124 114 40
BseYI C'CCAG_C
Cuts at: 1434 1859 2302 3060 3637 4133 1434
Size: 425 443 758 577 496 2178
Fragments arranged by size:
2178 758 577 496 443 425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1381 346
Size: 1035 3842
BsiEI CG_ry'CG
Cuts at: 104 431 2419 2465 3673 104
Size: 327 1988 46 1208 1308
Fragments arranged by size:
1988 1308 1208 327 46
BsiHKAI G_wGCw'C
Cuts at: 1045 1891 2669 2859 3651 1045
Size: 846 778 190 792 2271
Fragments arranged by size:
2271 846 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1773 1773
Size: 4877
Bsp1286I G_dGCh'C
Cuts at: 182 280 305 732 747 968 1045 1452 1676
Size: 98 25 427 15 221 77 407 224
Cuts at: 1676 1840 1891 2502 2595 2669 2859 2921 3651
Size: 164 51 611 93 74 190 62 730
Cuts at: 3651 182
Size: 1408
Fragments arranged by size:
1408 730 611 427 407 224 221 190
164 98 93 77 74 62 51 25
15
BspEI T'CCGG_A
Cuts at: 4097 4097
Size: 4877
BspHI T'CATG_A
Cuts at: 4053 4053
Size: 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 427 900 1927 2141 2443 2824 427
Size: 473 1027 214 302 381 2480
Fragments arranged by size:
2480 1027 473 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3169 3223 4707 4872 50
Size: 3119 54 1484 165 55
Fragments arranged by size:
3119 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1132 2789 1132
Size: 1657 3220
BsrFI r'CCGG_y
Cuts at: 2875 3056 3265 2875
Size: 181 209 4487
Fragments arranged by size:
4487 209 181
BssSI C'ACGA_G
Cuts at: 3148 3506 3148
Size: 358 4519
BstAPI GCAn_nnn'nTGC
Cuts at: 510 514 2254 510
Size: 4 1740 3133
Fragments arranged by size:
3133 1740 4
BstBI TT'CG_AA
Cuts at: 3238 3238
Size: 4877
BstEII G'GTnAC_C
Cuts at: 912 912
Size: 4877
BstXI CCAn_nnnn'nTGG
Cuts at: 843 975 843
Size: 132 4745
BstYI r'GATC_y
Cuts at: 1 1828 1902 2369 2392 2727 2973 3985 4071
Size: 1827 74 467 23 335 246 1012 86
Cuts at: 4071 4083 4429 1
Size: 12 346 449
Fragments arranged by size:
1827 1012 467 449 346 335 246 86
74 23 12
BtgI C'CryG_G
Cuts at: 107 320 603 1046 1591 107
Size: 213 283 443 545 3393
Fragments arranged by size:
3393 545 443 283 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 433 2827 3008 3171 433
Size: 2394 181 163 2139
Fragments arranged by size:
2394 2139 181 163
BtsI GCAGTG_nn'
Cuts at: 508 556 2120 508
Size: 48 1564 3265
Fragments arranged by size:
3265 1564 48
ClaI AT'CG_AT
Cuts at: 1634 1634
Size: 4877
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 771 806 771
Size: 35 4842
DraI TTT'AAA
Cuts at: 1876 1876
Size: 4877
DraIII CAC_nnn'GTG
Cuts at: 1118 1118
Size: 4877
DrdI GACnn_nn'nnGTC
Cuts at: 1426 2583 3441 1426
Size: 1157 858 2862
Fragments arranged by size:
2862 1157 858
EaeI y'GGCC_r
Cuts at: 194 224 413 701 814 1133 1535 1693 2462
Size: 30 189 288 113 319 402 158 769
Cuts at: 2462 2636 3027 3054 4399 194
Size: 174 391 27 1345 672
Fragments arranged by size:
1345 769 672 402 391 319 288 189
174 158 113 30 27
EagI C'GGCC_G
Cuts at: 2462 2462
Size: 4877
EarI CTCTTCn'nnn_
Cuts at: 1294 1508 2900 3110 1294
Size: 214 1392 210 3061
Fragments arranged by size:
3061 1392 214 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3085 3395 3541 4687 3085
Size: 310 146 1146 3275
Fragments arranged by size:
3275 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1889 1889
Size: 4877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 639 1541 2702 3134 3881 4149 271
Size: 368 902 1161 432 747 268 999
Fragments arranged by size:
1161 999 902 747 432 368 268
EcoO109I rG'GnC_Cy
Cuts at: 800 1248 1323 1428 1449 1459 1585 1597 1730
Size: 448 75 105 21 10 126 12 133
Cuts at: 1730 1836 2016 800
Size: 106 180 3661
Fragments arranged by size:
3661 448 180 133 126 106 105 75
21 12 10
EcoRI G'AATT_C
Cuts at: 1881 1881
Size: 4877
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4877
FspI TGC'GCA
Cuts at: 1111 2658 1111
Size: 1547 3330
FspAI rTGC'GCAy
Cuts at: 1111 1111
Size: 4877
HaeII r_GCGC'y
Cuts at: 190 412 594 932 1012 1095 2311 2559 3581
Size: 222 182 338 80 83 1216 248 1022
Cuts at: 3581 4560 190
Size: 979 507
Fragments arranged by size:
1216 1022 979 507 338 248 222 182
83 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2719 2749 2751 2781 2719
Size: 30 2 30 4815
Fragments arranged by size:
4815 30 30 2
HincII GTy'rAC
Cuts at: 477 1916 477
Size: 1439 3438
KasI G'GCGC_C
Cuts at: 1008 2307 2555 1008
Size: 1299 248 3330
Fragments arranged by size:
3330 1299 248
KpnI G_GTAC'C
Cuts at: 812 832 1897 812
Size: 20 1065 3792
Fragments arranged by size:
3792 1065 20
MluI A'CGCG_T
Cuts at: 4310 4310
Size: 4877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 176 374 980 1472 1681 1731 3548 3732 176
Size: 198 606 492 209 50 1817 184 1321
Fragments arranged by size:
1817 1321 606 492 209 198 184 50
MscI TGG'CCA
Cuts at: 196 415 703 1135 1537 2638 196
Size: 219 288 432 402 1101 2435
Fragments arranged by size:
2435 1101 432 402 288 219
MslI CAynn'nnrTG
Cuts at: 289 457 532 841 961 973 2866 2993 289
Size: 168 75 309 120 12 1893 127 2173
Fragments arranged by size:
2173 1893 309 168 127 120 75 12
MspA1I CmG'CkG
Cuts at: 298 330 605 1863 1988 2302 2662 3675 3920
Size: 32 275 1258 125 314 360 1013 245
Cuts at: 3920 298
Size: 1255
Fragments arranged by size:
1258 1255 1013 360 314 275 245 125
32
NaeI GCC'GGC
Cuts at: 3058 3058
Size: 4877
NarI GG'CG_CC
Cuts at: 1009 2308 2556 1009
Size: 1299 248 3330
Fragments arranged by size:
3330 1299 248
NcoI C'CATG_G
Cuts at: 107 1046 107
Size: 939 3938
NgoMIV G'CCGG_C
Cuts at: 3056 3056
Size: 4877
NspI r_CATG'y
Cuts at: 456 517 1287 1930 2430 3337 456
Size: 61 770 643 500 907 1996
Fragments arranged by size:
1996 907 770 643 500 61
PciI A'CATG_T
Cuts at: 3333 3333
Size: 4877
PflMI CCAn_nnn'nTGG
Cuts at: 203 572 1554 203
Size: 369 982 3526
Fragments arranged by size:
3526 982 369
PfoI T'CCnGG_A
Cuts at: 1610 1610
Size: 4877
PmeI GTTT'AAAC
Cuts at: 1876 1876
Size: 4877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4845
PpuMI rG'GwC_Cy
Cuts at: 1248 1323 1428 1459 1585 1597 1730 1248
Size: 75 105 31 126 12 133 4395
Fragments arranged by size:
4395 133 126 105 75 31 12
PspOMI G'GGCC_C
Cuts at: 728 1448 1836 728
Size: 720 388 3769
Fragments arranged by size:
3769 720 388
PstI C_TGCA'G
Cuts at: 911 1924 2609 911
Size: 1013 685 3179
Fragments arranged by size:
3179 1013 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4877
PvuII CAG'CTG
Cuts at: 298 330 1863 2302 2662 298
Size: 32 1533 439 360 2513
Fragments arranged by size:
2513 1533 439 360 32
RsrII CG'GwC_CG
Cuts at: 3072 3072
Size: 4877
SacI G_AGCT'C
Cuts at: 1891 1891
Size: 4877
SacII CC_GC'GG
Cuts at: 606 606
Size: 4877
SalI G'TCGA_C
Cuts at: 1914 1914
Size: 4877
SanDI GG'GwC_CC
Cuts at: 1248 1585 1730 1248
Size: 337 145 4395
Fragments arranged by size:
4395 337 145
SapI GCTCTTCn'nnn_
Cuts at: 1294 2900 3110 1294
Size: 1606 210 3061
Fragments arranged by size:
3061 1606 210
SbfI CC_TGCA'GG
Cuts at: 911 1924 911
Size: 1013 3864
ScaI AGT'ACT
Cuts at: 4394 4394
Size: 4877
SfcI C'TryA_G
Cuts at: 33 907 1920 2605 3598 3789 33
Size: 874 1013 685 993 191 1121
Fragments arranged by size:
1121 1013 993 874 685 191
SfoI GGC'GCC
Cuts at: 1010 2309 2557 1010
Size: 1299 248 3330
Fragments arranged by size:
3330 1299 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4877
SmaI CCC'GGG
Cuts at: 1899 1899
Size: 4877
SmlI C'TyrA_G
Cuts at: 994 1300 2032 2947 3439 3701 994
Size: 306 732 915 492 262 2170
Fragments arranged by size:
2170 915 732 492 306 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4877
SphI G_CATG'C
Cuts at: 517 1930 2430 517
Size: 1413 500 2964
Fragments arranged by size:
2964 1413 500
StuI AGG'CCT
Cuts at: 139 139
Size: 4877
StyI C'CwwG_G
Cuts at: 107 803 1046 1725 2011 2365 107
Size: 696 243 679 286 354 2619
Fragments arranged by size:
2619 696 679 354 286 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2460 2593 3052 3261 2460
Size: 133 459 209 4076
Fragments arranged by size:
4076 459 209 133
TatI w'GTAC_w
Cuts at: 4392 4392
Size: 4877
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4394 4426 4394
Size: 32 4845
Tth111I GACn'n_nGTC
Cuts at: 2674 2674
Size: 4877
XbaI T'CTAG_A
Cuts at: 1908 1908
Size: 4877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 318 2372 318
Size: 2054 2823
XmaI C'CCGG_G
Cuts at: 1897 1897
Size: 4877
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BfrBI
BmtI BsaXI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I
EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI