Restriction map for
pF1KB9540
(Circular) (Six-base) MAPSORT of: pF1KB9540.seq Check: 4850 from: 1 to: 4166
pF1KB9540 4166 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 1204 1204
Size: 4166
Acc65I G'GTAC_C
Cuts at: 989 1182 989
Size: 193 3973
AclI AA'CG_TT
Cuts at: 4006 4006
Size: 4166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 695 734 1991 2423 3170 3438 209
Size: 486 39 1257 432 747 268 937
Fragments arranged by size:
1257 937 747 486 432 268 39
AflIII A'CryG_T
Cuts at: 1518 2622 3599 1518
Size: 1104 977 2085
Fragments arranged by size:
2085 1104 977
AgeI A'CCGG_T
Cuts at: 2554 2554
Size: 4166
AhdI GACnn_n'nnGTC
Cuts at: 156 156
Size: 4166
AlwNI CAG_nnn'CTG
Cuts at: 1107 1471 3038 1107
Size: 364 1567 2235
Fragments arranged by size:
2235 1567 364
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 1170 3519 3775 1170
Size: 2349 256 1561
Fragments arranged by size:
2349 1561 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 1186 1186
Size: 4166
AvrII C'CTAG_G
Cuts at: 198 198
Size: 4166
BamHI G'GATC_C
Cuts at: 1191 1191
Size: 4166
BanI G'GyrC_C
Cuts at: 989 1182 1596 1844 1879 989
Size: 193 414 248 35 3276
Fragments arranged by size:
3276 414 248 193 35
BanII G_rGCy'C
Cuts at: 280 781 829 1180 2210 280
Size: 501 48 351 1030 2236
Fragments arranged by size:
2236 1030 501 351 48
BbeI G_GCGC'C
Cuts at: 1600 1848 1600
Size: 248 3918
BbsI GAAGACnn'nnnn_
Cuts at: 746 746
Size: 4166
BbvCI CC'TCA_GC
Cuts at: 803 803
Size: 4166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 379 413 550 584 379
Size: 34 137 34 3961
Fragments arranged by size:
3961 137 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2064 2825 4166 2064
Size: 761 1341 2064
Fragments arranged by size:
2064 1341 761
BclI T'GATC_A
Cuts at: 1686 1686
Size: 4166
BglII A'GATC_T
Cuts at: 1681 3718 1681
Size: 2037 2129
BlpI GC'TnA_GC
Cuts at: 1278 1278
Size: 4166
Bme1580I G_kGCm'C
Cuts at: 1791 1884 2940 1791
Size: 93 1056 3017
Fragments arranged by size:
3017 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1378 1459 1794 3938 1378
Size: 81 335 2144 1606
Fragments arranged by size:
2144 1606 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 241 611 836 920 240
Size: 1 370 225 84 3486
Fragments arranged by size:
3486 370 225 84 1
Bpu10I CC'TnA_GC
Cuts at: 127 269 803 127
Size: 142 534 3490
Fragments arranged by size:
3490 534 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 2221 2713 3011 1342
Size: 879 492 298 2497
Fragments arranged by size:
2497 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 208 803 3870 208
Size: 595 3067 504
Fragments arranged by size:
3067 595 504
BsaAI yAC'GTr
Cuts at: 1519 1519
Size: 4166
BsaBI GATnn'nnATC
Cuts at: 6 1680 3717 6
Size: 1674 2037 455
Fragments arranged by size:
2037 1674 455
BsaHI Gr'CG_yC
Cuts at: 1597 1845 2547 4043 1597
Size: 248 702 1496 1720
Fragments arranged by size:
1720 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1355 1876 2554 2828 2975 3386 1355
Size: 521 678 274 147 411 2135
Fragments arranged by size:
2135 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1087 1117 1087
Size: 30 4136
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 270 791 270
Size: 521 3645
BseYI C'CCAG_C
Cuts at: 651 1591 2349 2926 3422 651
Size: 940 758 577 496 1395
Fragments arranged by size:
1395 940 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1708 1754 2962 104
Size: 1604 46 1208 1308
Fragments arranged by size:
1604 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1180 1958 2148 2940 1180
Size: 778 190 792 2406
Fragments arranged by size:
2406 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 280 781 829 1180 1791 1884 1958 2148 2210
Size: 501 48 351 611 93 74 190 62
Cuts at: 2210 2940 280
Size: 730 1506
Fragments arranged by size:
1506 730 611 501 351 190 93 74
62 48
BspEI T'CCGG_A
Cuts at: 3386 3386
Size: 4166
BspHI T'CATG_A
Cuts at: 3342 3342
Size: 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1216 1430 1732 2113 1216
Size: 214 302 381 3269
Fragments arranged by size:
3269 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2458 2512 3996 4161 50
Size: 2408 54 1484 165 55
Fragments arranged by size:
2408 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2078 2078
Size: 4166
BsrFI r'CCGG_y
Cuts at: 2164 2345 2554 2164
Size: 181 209 3776
Fragments arranged by size:
3776 209 181
BssSI C'ACGA_G
Cuts at: 2437 2795 2437
Size: 358 3808
BstAPI GCAn_nnn'nTGC
Cuts at: 1543 1543
Size: 4166
BstBI TT'CG_AA
Cuts at: 2527 2527
Size: 4166
BstYI r'GATC_y
Cuts at: 1 1097 1191 1658 1681 2016 2262 3274 3360
Size: 1096 94 467 23 335 246 1012 86
Cuts at: 3360 3372 3718 1
Size: 12 346 449
Fragments arranged by size:
1096 1012 467 449 346 335 246 94
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2116 2297 2460 2116
Size: 181 163 3822
Fragments arranged by size:
3822 181 163
BtsI GCAGTG_nn'
Cuts at: 663 698 1409 663
Size: 35 711 3420
Fragments arranged by size:
3420 711 35
DraI TTT'AAA
Cuts at: 1165 1165
Size: 4166
DrdI GACnn_nn'nnGTC
Cuts at: 1872 2730 1872
Size: 858 3308
EaeI y'GGCC_r
Cuts at: 227 531 1088 1751 1925 2316 2343 3688 227
Size: 304 557 663 174 391 27 1345 705
Fragments arranged by size:
1345 705 663 557 391 304 174 27
EagI C'GGCC_G
Cuts at: 1751 1751
Size: 4166
EarI CTCTTCn'nnn_
Cuts at: 599 997 1118 2189 2399 599
Size: 398 121 1071 210 2366
Fragments arranged by size:
2366 1071 398 210 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1024 1093 2374 2684 2830 3976 1024
Size: 69 1281 310 146 1146 1214
Fragments arranged by size:
1281 1214 1146 310 146 69
EcoICRI GAG'CTC
Cuts at: 1178 1178
Size: 4166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 240 241 611 695 734 836 920 1991
Size: 31 1 370 84 39 102 84 1071
Cuts at: 1991 2423 3170 3438 209
Size: 432 747 268 937
Fragments arranged by size:
1071 937 747 432 370 268 102 84
84 39 31 1
EcoNI CCTnn'n_nnAGG
Cuts at: 487 819 1090 487
Size: 332 271 3563
Fragments arranged by size:
3563 332 271
EcoO109I rG'GnC_Cy
Cuts at: 612 1155 1305 612
Size: 543 150 3473
Fragments arranged by size:
3473 543 150
EcoRI G'AATT_C
Cuts at: 1170 1170
Size: 4166
EcoRV GAT'ATC
Cuts at: 669 669
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 4166
FspI TGC'GCA
Cuts at: 1947 1947
Size: 4166
HaeII r_GCGC'y
Cuts at: 1600 1848 2870 3849 1600
Size: 248 1022 979 1917
Fragments arranged by size:
1917 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 328 360 2008 2038 2040 2070 142
Size: 32 154 32 1648 30 2 30 2238
Fragments arranged by size:
2238 1648 154 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1205 1205
Size: 4166
KasI G'GCGC_C
Cuts at: 1596 1844 1596
Size: 248 3918
KpnI G_GTAC'C
Cuts at: 993 1186 993
Size: 193 3973
MfeI C'AATT_G
Cuts at: 527 527
Size: 4166
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 434 2837 3021 344
Size: 90 2403 184 1489
Fragments arranged by size:
2403 1489 184 90
MscI TGG'CCA
Cuts at: 229 533 1090 1927 229
Size: 304 557 837 2468
Fragments arranged by size:
2468 837 557 304
MslI CAynn'nnrTG
Cuts at: 772 2155 2282 772
Size: 1383 127 2656
Fragments arranged by size:
2656 1383 127
MspA1I CmG'CkG
Cuts at: 1277 1591 1951 2964 3209 1277
Size: 314 360 1013 245 2234
Fragments arranged by size:
2234 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 4166
NarI GG'CG_CC
Cuts at: 1597 1845 1597
Size: 248 3918
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 4166
NspI r_CATG'y
Cuts at: 1219 1719 2626 1219
Size: 500 907 2759
Fragments arranged by size:
2759 907 500
PasI CC'CwG_GG
Cuts at: 607 607
Size: 4166
PciI A'CATG_T
Cuts at: 2622 2622
Size: 4166
PfoI T'CCnGG_A
Cuts at: 346 488 897 346
Size: 142 409 3615
Fragments arranged by size:
3615 409 142
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 4134
PpuMI rG'GwC_Cy
Cuts at: 612 1155 612
Size: 543 3623
PstI C_TGCA'G
Cuts at: 1213 1898 1213
Size: 685 3481
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4166
PvuII CAG'CTG
Cuts at: 1591 1951 1591
Size: 360 3806
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 1180 1180
Size: 4166
SalI G'TCGA_C
Cuts at: 1203 1203
Size: 4166
SanDI GG'GwC_CC
Cuts at: 1155 1155
Size: 4166
SapI GCTCTTCn'nnn_
Cuts at: 2189 2399 2189
Size: 210 3956
SbfI CC_TGCA'GG
Cuts at: 1213 1213
Size: 4166
ScaI AGT'ACT
Cuts at: 3683 3683
Size: 4166
SexAI A'CCwGG_T
Cuts at: 563 563
Size: 4166
SfcI C'TryA_G
Cuts at: 33 1209 1894 2887 3078 33
Size: 1176 685 993 191 1121
Fragments arranged by size:
1176 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1598 1846 1598
Size: 248 3918
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SmaI CCC'GGG
Cuts at: 1188 1188
Size: 4166
SmlI C'TyrA_G
Cuts at: 1321 2236 2728 2990 1321
Size: 915 492 262 2497
Fragments arranged by size:
2497 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4166
SphI G_CATG'C
Cuts at: 1219 1719 1219
Size: 500 3666
StyI C'CwwG_G
Cuts at: 198 1091 1300 1654 198
Size: 893 209 354 2710
Fragments arranged by size:
2710 893 354 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 1749 1882 2341 2550 127
Size: 1622 133 459 209 1743
Fragments arranged by size:
1743 1622 459 209 133
TatI w'GTAC_w
Cuts at: 3681 3681
Size: 4166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4134
Tth111I GACn'n_nGTC
Cuts at: 1963 1963
Size: 4166
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1661 1661
Size: 4166
XmaI C'CCGG_G
Cuts at: 1186 1186
Size: 4166
XmnI GAAnn'nnTTC
Cuts at: 385 385
Size: 4166
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI BaeI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI