Restriction map for
pF1KB9545
(Circular) (Six-base) MAPSORT of: pF1KB9545.seq Check: 9138 from: 1 to: 4742
pF1KB9545 4742 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1147 1147
Size: 4742
AccI GT'mk_AC
Cuts at: 1780 1780
Size: 4742
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 689 704 1621 2567 2999 3352 4014 506
Size: 183 15 917 946 432 353 662 1234
Fragments arranged by size:
1234 946 917 662 432 353 183 15
AflIII A'CryG_T
Cuts at: 216 1448 2094 3894 4175 216
Size: 1232 646 1800 281 783
Fragments arranged by size:
1800 1232 783 646 281
AhdI GACnn_n'nnGTC
Cuts at: 1550 1550
Size: 4742
AleI CACnn'nnGTG
Cuts at: 1488 1488
Size: 4742
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1682 1714 1682
Size: 32 4710
AlwNI CAG_nnn'CTG
Cuts at: 2047 3485 2047
Size: 1438 3304
ApaLI G'TGCA_C
Cuts at: 1481 3580 1481
Size: 2099 2643
ApoI r'AATT_y
Cuts at: 261 699 1096 1398 1424 1718 1746 4095 4351
Size: 438 397 302 26 294 28 2349 256
Cuts at: 4351 261
Size: 652
Fragments arranged by size:
2349 652 438 397 302 294 256 28
26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 303 1762 303
Size: 1459 3283
BamHI G'GATC_C
Cuts at: 1767 1767
Size: 4742
BanI G'GyrC_C
Cuts at: 1758 2172 2420 2455 1758
Size: 414 248 35 4045
Fragments arranged by size:
4045 414 248 35
BanII G_rGCy'C
Cuts at: 197 1220 1756 2786 197
Size: 1023 536 1030 2153
Fragments arranged by size:
2153 1030 1023 536
BbeI G_GCGC'C
Cuts at: 2176 2424 2176
Size: 248 4494
BbsI GAAGACnn'nnnn_
Cuts at: 1018 1018
Size: 4742
BbvCI CC'TCA_GC
Cuts at: 1036 1036
Size: 4742
BciVI GTATCCnnnnn_n'
Cuts at: 2640 3696 4742 2640
Size: 1056 1046 2640
Fragments arranged by size:
2640 1056 1046
BclI T'GATC_A
Cuts at: 333 2262 333
Size: 1929 2813
BglII A'GATC_T
Cuts at: 2257 4294 2257
Size: 2037 2705
BlpI GC'TnA_GC
Cuts at: 191 233 1854 191
Size: 42 1621 3079
Fragments arranged by size:
3079 1621 42
Bme1580I G_kGCm'C
Cuts at: 1485 2367 2460 3584 1485
Size: 882 93 1124 2643
Fragments arranged by size:
2643 1124 882 93
BmrI ACTGGGnnnn_n'
Cuts at: 1479 1542 1954 2035 2370 4514 1479
Size: 63 412 81 335 2144 1707
Fragments arranged by size:
2144 1707 412 335 81 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 4742
Bpu10I CC'TnA_GC
Cuts at: 1036 1574 1036
Size: 538 4204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1918 2797 3511 3809 1918
Size: 879 714 298 2851
Fragments arranged by size:
2851 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 376 4446 376
Size: 4070 672
BsaAI yAC'GTr
Cuts at: 1080 2095 1080
Size: 1015 3727
BsaBI GATnn'nnATC
Cuts at: 6 2256 4293 6
Size: 2250 2037 455
Fragments arranged by size:
2250 2037 455
BsaHI Gr'CG_yC
Cuts at: 2173 2421 3123 4619 2173
Size: 248 702 1496 2296
Fragments arranged by size:
2296 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1931 2452 3130 3541 3688 3962 1931
Size: 521 678 411 147 274 2711
Fragments arranged by size:
2711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1682 1712 1682
Size: 30 4712
BseYI C'CCAG_C
Cuts at: 1121 2167 2925 3590 3998 1121
Size: 1046 758 665 408 1865
Fragments arranged by size:
1865 1046 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2284 2330 3560 104
Size: 2180 46 1230 1286
Fragments arranged by size:
2180 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 197 807 1485 1541 1678 1756 2534 2724 3584
Size: 610 678 56 137 78 778 190 860
Cuts at: 3584 197
Size: 1355
Fragments arranged by size:
1355 860 778 678 610 190 137 78
56
Bsp1286I G_dGCh'C
Cuts at: 197 807 1220 1485 1541 1678 1756 2367 2460
Size: 610 413 265 56 137 78 611 93
Cuts at: 2460 2534 2724 2786 3584 197
Size: 74 190 62 798 1355
Fragments arranged by size:
1355 798 611 610 413 265 190 137
93 78 74 62 56
BspHI T'CATG_A
Cuts at: 378 3174 378
Size: 2796 1946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1147 1792 2006 2308 2689 1147
Size: 645 214 302 381 3200
Fragments arranged by size:
3200 645 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3034 3088 4572 4737 50
Size: 2984 54 1484 165 55
Fragments arranged by size:
2984 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 559 2654 559
Size: 2095 2647
BsrFI r'CCGG_y
Cuts at: 1154 1502 2740 2921 1154
Size: 348 1238 181 2975
Fragments arranged by size:
2975 1238 348 181
BssSI C'ACGA_G
Cuts at: 614 3013 3721 614
Size: 2399 708 1635
Fragments arranged by size:
2399 1635 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2119 2119
Size: 4742
BstBI TT'CG_AA
Cuts at: 641 3103 641
Size: 2462 2280
BstYI r'GATC_y
Cuts at: 1 1767 2234 2257 2592 2838 3144 3156 3242
Size: 1766 467 23 335 246 306 12 86
Cuts at: 3242 4294 1
Size: 1052 449
Fragments arranged by size:
1766 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4742
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2692 2873 3036 2692
Size: 181 163 4398
Fragments arranged by size:
4398 181 163
BtsI GCAGTG_nn'
Cuts at: 407 1146 1205 1985 407
Size: 739 59 780 3164
Fragments arranged by size:
3164 780 739 59
DraI TTT'AAA
Cuts at: 1029 1741 1029
Size: 712 4030
DrdI GACnn_nn'nnGTC
Cuts at: 2448 3792 2448
Size: 1344 3398
EaeI y'GGCC_r
Cuts at: 2327 2501 2892 2919 4264 2327
Size: 174 391 27 1345 2805
Fragments arranged by size:
2805 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2327 2327
Size: 4742
EarI CTCTTCn'nnn_
Cuts at: 122 525 1640 2765 2975 122
Size: 403 1115 1125 210 1889
Fragments arranged by size:
1889 1125 1115 403 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2950 3692 3838 4552 2950
Size: 742 146 714 3140
Fragments arranged by size:
3140 742 714 146
EcoICRI GAG'CTC
Cuts at: 195 1754 195
Size: 1559 3183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 689 704 1621 2567 2999 3352 4014 506
Size: 183 15 917 946 432 353 662 1234
Fragments arranged by size:
1234 946 917 662 432 353 183 15
EcoO109I rG'GnC_Cy
Cuts at: 1266 1881 1266
Size: 615 4127
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4742
FspI TGC'GCA
Cuts at: 2523 2523
Size: 4742
HaeII r_GCGC'y
Cuts at: 2176 2424 3654 4425 2176
Size: 248 1230 771 2493
Fragments arranged by size:
2493 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 915 947 1331 1363 1536 1568 1645
Size: 32 429 32 384 32 173 32 77
Cuts at: 1645 1677 2584 2614 2616 2646 454
Size: 32 907 30 2 30 2550
Fragments arranged by size:
2550 907 429 384 173 77 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1781 1781
Size: 4742
KasI G'GCGC_C
Cuts at: 2172 2420 2172
Size: 248 4494
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3501 3685 3501
Size: 184 4558
MscI TGG'CCA
Cuts at: 2503 2503
Size: 4742
MslI CAynn'nnrTG
Cuts at: 221 1449 1488 1669 2731 2858 221
Size: 1228 39 181 1062 127 2105
Fragments arranged by size:
2105 1228 1062 181 127 39
MspA1I CmG'CkG
Cuts at: 1040 1121 1853 2167 2527 3311 3556 1040
Size: 81 732 314 360 784 245 2226
Fragments arranged by size:
2226 784 732 360 314 245 81
NaeI GCC'GGC
Cuts at: 1504 2923 1504
Size: 1419 3323
NarI GG'CG_CC
Cuts at: 2173 2421 2173
Size: 248 4494
NcoI C'CATG_G
Cuts at: 107 107
Size: 4742
NgoMIV G'CCGG_C
Cuts at: 1502 2921 1502
Size: 1419 3323
NspI r_CATG'y
Cuts at: 220 1452 1795 2295 3898 220
Size: 1232 343 500 1603 1064
Fragments arranged by size:
1603 1232 1064 500 343
PciI A'CATG_T
Cuts at: 216 1448 3894 216
Size: 1232 2446 1064
Fragments arranged by size:
2446 1232 1064
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PmlI CAC'GTG
Cuts at: 1080 1080
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1682 1714 3188 3220 1682
Size: 32 1474 32 3204
Fragments arranged by size:
3204 1474 32 32
PpuMI rG'GwC_Cy
Cuts at: 1266 1266
Size: 4742
PstI C_TGCA'G
Cuts at: 1789 2474 1789
Size: 685 4057
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 1040 1121 2167 2527 1040
Size: 81 1046 360 3255
Fragments arranged by size:
3255 1046 360 81
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 197 1756 197
Size: 1559 3183
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 4259 4259
Size: 4742
SfcI C'TryA_G
Cuts at: 33 1180 1507 1785 2470 3438 3629 33
Size: 1147 327 278 685 968 191 1146
Fragments arranged by size:
1147 1146 968 685 327 278 191
SfoI GGC'GCC
Cuts at: 2174 2422 2174
Size: 248 4494
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 305 1764 305
Size: 1459 3283
SmlI C'TyrA_G
Cuts at: 1897 2812 3526 3788 1897
Size: 915 714 262 2851
Fragments arranged by size:
2851 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 1795 2295 1795
Size: 500 4242
StyI C'CwwG_G
Cuts at: 107 1876 2230 107
Size: 1769 354 2619
Fragments arranged by size:
2619 1769 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1335 2325 2458 2917 3126 1335
Size: 990 133 459 209 2951
Fragments arranged by size:
2951 990 459 209 133
TatI w'GTAC_w
Cuts at: 577 754 4257 577
Size: 177 3503 1062
Fragments arranged by size:
3503 1062 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1477 1509 4259 4291 1477
Size: 32 2750 32 1928
Fragments arranged by size:
2750 1928 32 32
Tth111I GACn'n_nGTC
Cuts at: 2539 2539
Size: 4742
XbaI T'CTAG_A
Cuts at: 1773 1773
Size: 4742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 796 1297 2237 796
Size: 501 940 3301
Fragments arranged by size:
3301 940 501
XmaI C'CCGG_G
Cuts at: 303 1762 303
Size: 1459 3283
XmnI GAAnn'nnTTC
Cuts at: 492 702 1634 492
Size: 210 932 3600
Fragments arranged by size:
3600 932 210
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AlfI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BpmI BseRI
BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI