Restriction map for
pF1KB9547
(Circular) (Six-base) MAPSORT of: pF1KB9547.seq Check: 5373 from: 1 to: 3443
pF1KB9547 3443 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 481 481
Size: 3443
Acc65I G'GTAC_C
Cuts at: 459 459
Size: 3443
AclI AA'CG_TT
Cuts at: 3283 3283
Size: 3443
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 1700 2447 2715 1268
Size: 432 747 268 1996
Fragments arranged by size:
1996 747 432 268
AflIII A'CryG_T
Cuts at: 795 1899 2876 795
Size: 1104 977 1362
Fragments arranged by size:
1362 1104 977
AgeI A'CCGG_T
Cuts at: 1831 1831
Size: 3443
AhdI GACnn_n'nnGTC
Cuts at: 301 301
Size: 3443
AleI CACnn'nnGTG
Cuts at: 232 232
Size: 3443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 250 250
Size: 3443
AlwNI CAG_nnn'CTG
Cuts at: 748 2315 748
Size: 1567 1876
ApaLI G'TGCA_C
Cuts at: 330 2213 330
Size: 1883 1560
ApoI r'AATT_y
Cuts at: 447 2796 3052 447
Size: 2349 256 838
Fragments arranged by size:
2349 838 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3443
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3443
AvaI C'yCGr_G
Cuts at: 463 463
Size: 3443
BamHI G'GATC_C
Cuts at: 468 468
Size: 3443
BanI G'GyrC_C
Cuts at: 459 873 1121 1156 459
Size: 414 248 35 2746
Fragments arranged by size:
2746 414 248 35
BanII G_rGCy'C
Cuts at: 388 457 1487 388
Size: 69 1030 2344
Fragments arranged by size:
2344 1030 69
BbeI G_GCGC'C
Cuts at: 877 1125 877
Size: 248 3195
BbsI GAAGACnn'nnnn_
Cuts at: 174 174
Size: 3443
BciVI GTATCCnnnnn_n'
Cuts at: 1341 2102 3443 1341
Size: 761 1341 1341
Fragments arranged by size:
1341 1341 761
BclI T'GATC_A
Cuts at: 963 963
Size: 3443
BglII A'GATC_T
Cuts at: 958 2995 958
Size: 2037 1406
BlpI GC'TnA_GC
Cuts at: 555 555
Size: 3443
Bme1580I G_kGCm'C
Cuts at: 334 1068 1161 2217 334
Size: 734 93 1056 1560
Fragments arranged by size:
1560 1056 734 93
BmrI ACTGGGnnnn_n'
Cuts at: 655 736 1071 3215 655
Size: 81 335 2144 883
Fragments arranged by size:
2144 883 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1810 1810
Size: 3443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1498 1990 2288 619
Size: 879 492 298 1774
Fragments arranged by size:
1774 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 309 3147 309
Size: 2838 605
BsaAI yAC'GTr
Cuts at: 796 796
Size: 3443
BsaBI GATnn'nnATC
Cuts at: 6 957 2994 6
Size: 951 2037 455
Fragments arranged by size:
2037 951 455
BsaHI Gr'CG_yC
Cuts at: 874 1122 1824 3320 874
Size: 248 702 1496 997
Fragments arranged by size:
1496 997 702 248
BsaWI w'CCGG_w
Cuts at: 632 1153 1831 2105 2252 2663 632
Size: 521 678 274 147 411 1412
Fragments arranged by size:
1412 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 868 1626 2203 2699 868
Size: 758 577 496 1612
Fragments arranged by size:
1612 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 210
Size: 3443
BsiEI CG_ry'CG
Cuts at: 104 985 1031 2239 104
Size: 881 46 1208 1308
Fragments arranged by size:
1308 1208 881 46
BsiHKAI G_wGCw'C
Cuts at: 334 388 457 1235 1425 2217 334
Size: 54 69 778 190 792 1560
Fragments arranged by size:
1560 792 778 190 69 54
Bsp1286I G_dGCh'C
Cuts at: 334 388 457 1068 1161 1235 1425 1487 2217
Size: 54 69 611 93 74 190 62 730
Cuts at: 2217 334
Size: 1560
Fragments arranged by size:
1560 730 611 190 93 74 69 62
54
BspEI T'CCGG_A
Cuts at: 2663 2663
Size: 3443
BspHI T'CATG_A
Cuts at: 2619 2619
Size: 3443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 493 707 1009 1390 493
Size: 214 302 381 2546
Fragments arranged by size:
2546 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1735 1789 3273 3438 50
Size: 1685 54 1484 165 55
Fragments arranged by size:
1685 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1355 1355
Size: 3443
BsrFI r'CCGG_y
Cuts at: 1441 1622 1831 1441
Size: 181 209 3053
Fragments arranged by size:
3053 209 181
BssSI C'ACGA_G
Cuts at: 1714 2072 1714
Size: 358 3085
BstAPI GCAn_nnn'nTGC
Cuts at: 820 820
Size: 3443
BstBI TT'CG_AA
Cuts at: 1804 1804
Size: 3443
BstYI r'GATC_y
Cuts at: 1 262 468 935 958 1293 1539 2551 2637
Size: 261 206 467 23 335 246 1012 86
Cuts at: 2637 2649 2995 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 261 246 206
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1393 1574 1737 1393
Size: 181 163 3099
Fragments arranged by size:
3099 181 163
BtsI GCAGTG_nn'
Cuts at: 686 686
Size: 3443
DraI TTT'AAA
Cuts at: 442 442
Size: 3443
DrdI GACnn_nn'nnGTC
Cuts at: 1149 2007 1149
Size: 858 2585
EaeI y'GGCC_r
Cuts at: 1028 1202 1593 1620 2965 1028
Size: 174 391 27 1345 1506
Fragments arranged by size:
1506 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1028 1028
Size: 3443
EarI CTCTTCn'nnn_
Cuts at: 406 427 1466 1676 406
Size: 21 1039 210 2173
Fragments arranged by size:
2173 1039 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1651 1961 2107 3253 1651
Size: 310 146 1146 1841
Fragments arranged by size:
1841 1146 310 146
EcoICRI GAG'CTC
Cuts at: 386 455 386
Size: 69 3374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 1700 2447 2715 1268
Size: 432 747 268 1996
Fragments arranged by size:
1996 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 295 582 295
Size: 287 3156
EcoRI G'AATT_C
Cuts at: 447 447
Size: 3443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1462 1462
Size: 3443
FspI TGC'GCA
Cuts at: 238 1224 238
Size: 986 2457
FspAI rTGC'GCAy
Cuts at: 238 238
Size: 3443
HaeII r_GCGC'y
Cuts at: 279 877 1125 2147 3126 279
Size: 598 248 1022 979 596
Fragments arranged by size:
1022 979 598 596 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 287 319 360 392 1285 1315 1317 1347 287
Size: 32 41 32 893 30 2 30 2383
Fragments arranged by size:
2383 893 41 32 32 30 30 2
HincII GTy'rAC
Cuts at: 482 482
Size: 3443
KasI G'GCGC_C
Cuts at: 873 1121 873
Size: 248 3195
KpnI G_GTAC'C
Cuts at: 463 463
Size: 3443
MluI A'CGCG_T
Cuts at: 2876 2876
Size: 3443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2114 2298 2114
Size: 184 3259
MscI TGG'CCA
Cuts at: 1204 1204
Size: 3443
MslI CAynn'nnrTG
Cuts at: 232 1432 1559 232
Size: 1200 127 2116
Fragments arranged by size:
2116 1200 127
MspA1I CmG'CkG
Cuts at: 554 868 1228 2241 2486 554
Size: 314 360 1013 245 1511
Fragments arranged by size:
1511 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1624 1624
Size: 3443
NarI GG'CG_CC
Cuts at: 874 1122 874
Size: 248 3195
NcoI C'CATG_G
Cuts at: 107 107
Size: 3443
NdeI CA'TA_TG
Cuts at: 241 241
Size: 3443
NgoMIV G'CCGG_C
Cuts at: 1622 1622
Size: 3443
NspI r_CATG'y
Cuts at: 496 996 1903 496
Size: 500 907 2036
Fragments arranged by size:
2036 907 500
PciI A'CATG_T
Cuts at: 1899 1899
Size: 3443
PfoI T'CCnGG_A
Cuts at: 338 338
Size: 3443
PmeI GTTT'AAAC
Cuts at: 442 442
Size: 3443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2582 2614 2582
Size: 32 3411
PpuMI rG'GwC_Cy
Cuts at: 295 295
Size: 3443
PstI C_TGCA'G
Cuts at: 490 1175 490
Size: 685 2758
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3443
PvuII CAG'CTG
Cuts at: 868 1228 868
Size: 360 3083
RsrII CG'GwC_CG
Cuts at: 1638 1638
Size: 3443
SacI G_AGCT'C
Cuts at: 388 457 388
Size: 69 3374
SalI G'TCGA_C
Cuts at: 480 480
Size: 3443
SapI GCTCTTCn'nnn_
Cuts at: 1466 1676 1466
Size: 210 3233
SbfI CC_TGCA'GG
Cuts at: 490 490
Size: 3443
ScaI AGT'ACT
Cuts at: 2960 2960
Size: 3443
SfcI C'TryA_G
Cuts at: 33 486 1171 2164 2355 33
Size: 453 685 993 191 1121
Fragments arranged by size:
1121 993 685 453 191
SfoI GGC'GCC
Cuts at: 875 1123 875
Size: 248 3195
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3443
SmaI CCC'GGG
Cuts at: 465 465
Size: 3443
SmlI C'TyrA_G
Cuts at: 598 1513 2005 2267 598
Size: 915 492 262 1774
Fragments arranged by size:
1774 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3443
SphI G_CATG'C
Cuts at: 496 996 496
Size: 500 2943
StyI C'CwwG_G
Cuts at: 107 577 931 107
Size: 470 354 2619
Fragments arranged by size:
2619 470 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1026 1159 1618 1827 1026
Size: 133 459 209 2642
Fragments arranged by size:
2642 459 209 133
TatI w'GTAC_w
Cuts at: 2958 2958
Size: 3443
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 3411
Tth111I GACn'n_nGTC
Cuts at: 1240 1240
Size: 3443
XbaI T'CTAG_A
Cuts at: 474 474
Size: 3443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 938 938
Size: 3443
XmaI C'CCGG_G
Cuts at: 463 463
Size: 3443
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI